| Definition | Frankia sp. EAN1pec chromosome, complete genome. |
|---|---|
| Accession | NC_009921 |
| Length | 8,982,042 |
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The map label for this gene is braF [H]
Identifier: 158313875
GI number: 158313875
Start: 2452901
End: 2453737
Strand: Direct
Name: braF [H]
Synonym: Franean1_2040
Alternate gene names: 158313875
Gene position: 2452901-2453737 (Clockwise)
Preceding gene: 158313874
Following gene: 158313876
Centisome position: 27.31
GC content: 75.27
Gene sequence:
>837_bases GTGACCGGCCGGCCCATCCCCGGCCCGCGGCCGTCGACGACCCTCGGGCCGGTCCTGGACGTCCGCGACGTCACCCTGCG CTTCGGCGGGCTGACCAGCCTGGACGGCGTCTCGCTGCGCCAGGACCGGGGAAGCGTGCTGGCTGTCATCGGCCCGAACG GGGCCGGGAAGACGTCCCTGTTCAACTGCCTGACCGGTGCCTACCGGCCGCAGCGGGGCAGCGCGCGCTTCTGGCCGGCG GATGACGGCGTCGAGCTGGTCGGGCGGTCCCCGCACCGGGTCGCGCGGCTCGGTGTGGCCCGGACGTTCCAGAACATCCG CCTGTTTCCCGAGATGTCGGCGGTGGAGAACGTGCAGGTCGGCGTCGAGGTGCGGGCCCGGACGGGGGCGCTGCGCGGGC TGCTGCCGACCAGGGCCGTGCGGCGGGAGGAACGGGAGACCCTCGACCGCTGCCATGAGCTGCTCGACTTCGTCGGGCTG GGCCACCGTGCCGAGCGGCCGGCGGCCGCCCTGGCCTACGGGGAGCAGCGACGGCTGGAGATCGCCCGCGCGCTCGGCAC GTCCCCCTCGCTGCTGCTGCTCGACGAGCCGGCCGCCGGTGCCACCGCCGCGGAGCGCACGGACCTCGTCACGCTCATCC GCGCCATCGCGGGGCGGGGCATCTCGGTGCTCCTCATCGAGCACGACATCCGGCTGGTGGCCGCGGTCGCCCGCGCGATC GTCGTGCTCAACTTCGGCCGGGTGATCGCCGCGGGACGTCCCGACGAGGTTCGGGCCGACCCCGCCGTCGTCGAGGCCTA CCTGGGCGAACCGGACGAGAGGCGGGACGGGCCGTGA
Upstream 100 bases:
>100_bases GCCCCGGCCCGGCCGCCGCCCCGGAGCACCCGGCGGTTCTCACCGAGCGCGCCGGAGCCTCGGGACCAGCCGGGCCCGCA GCGTCGGCCGAGGCGGCGCG
Downstream 100 bases:
>100_bases CACCGGACGGGAACCCCCTCGACGGATCGCCACTCGAGAACATCGCGCCACTCGAGAACATCGCGCCACTCGAGAACATC GCGCCGCTGGAGAACATCTC
Product: ABC transporter-like protein
Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]
Alternate protein names: NA
Number of amino acids: Translated: 278; Mature: 277
Protein sequence:
>278_residues MTGRPIPGPRPSTTLGPVLDVRDVTLRFGGLTSLDGVSLRQDRGSVLAVIGPNGAGKTSLFNCLTGAYRPQRGSARFWPA DDGVELVGRSPHRVARLGVARTFQNIRLFPEMSAVENVQVGVEVRARTGALRGLLPTRAVRREERETLDRCHELLDFVGL GHRAERPAAALAYGEQRRLEIARALGTSPSLLLLDEPAAGATAAERTDLVTLIRAIAGRGISVLLIEHDIRLVAAVARAI VVLNFGRVIAAGRPDEVRADPAVVEAYLGEPDERRDGP
Sequences:
>Translated_278_residues MTGRPIPGPRPSTTLGPVLDVRDVTLRFGGLTSLDGVSLRQDRGSVLAVIGPNGAGKTSLFNCLTGAYRPQRGSARFWPA DDGVELVGRSPHRVARLGVARTFQNIRLFPEMSAVENVQVGVEVRARTGALRGLLPTRAVRREERETLDRCHELLDFVGL GHRAERPAAALAYGEQRRLEIARALGTSPSLLLLDEPAAGATAAERTDLVTLIRAIAGRGISVLLIEHDIRLVAAVARAI VVLNFGRVIAAGRPDEVRADPAVVEAYLGEPDERRDGP >Mature_277_residues TGRPIPGPRPSTTLGPVLDVRDVTLRFGGLTSLDGVSLRQDRGSVLAVIGPNGAGKTSLFNCLTGAYRPQRGSARFWPAD DGVELVGRSPHRVARLGVARTFQNIRLFPEMSAVENVQVGVEVRARTGALRGLLPTRAVRREERETLDRCHELLDFVGLG HRAERPAAALAYGEQRRLEIARALGTSPSLLLLDEPAAGATAAERTDLVTLIRAIAGRGISVLLIEHDIRLVAAVARAIV VLNFGRVIAAGRPDEVRADPAVVEAYLGEPDERRDGP
Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]
COG id: COG0411
COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component
Gene ontology:
Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 ABC transporter domain [H]
Homologues:
Organism=Homo sapiens, GI150417984, Length=247, Percent_Identity=29.5546558704453, Blast_Score=75, Evalue=5e-14, Organism=Homo sapiens, GI31657092, Length=232, Percent_Identity=25.4310344827586, Blast_Score=70, Evalue=2e-12, Organism=Homo sapiens, GI27881501, Length=236, Percent_Identity=22.4576271186441, Blast_Score=67, Evalue=3e-11, Organism=Homo sapiens, GI30795238, Length=236, Percent_Identity=22.4576271186441, Blast_Score=67, Evalue=3e-11, Organism=Homo sapiens, GI46592964, Length=203, Percent_Identity=27.0935960591133, Blast_Score=66, Evalue=3e-11, Organism=Homo sapiens, GI46592978, Length=203, Percent_Identity=27.0935960591133, Blast_Score=66, Evalue=4e-11, Organism=Homo sapiens, GI46592956, Length=202, Percent_Identity=27.2277227722772, Blast_Score=66, Evalue=4e-11, Organism=Homo sapiens, GI46592915, Length=203, Percent_Identity=27.0935960591133, Blast_Score=66, Evalue=4e-11, Organism=Homo sapiens, GI46592898, Length=203, Percent_Identity=27.0935960591133, Blast_Score=66, Evalue=4e-11, Organism=Homo sapiens, GI46592971, Length=203, Percent_Identity=27.0935960591133, Blast_Score=66, Evalue=4e-11, Organism=Escherichia coli, GI1789864, Length=255, Percent_Identity=44.7058823529412, Blast_Score=216, Evalue=2e-57, Organism=Escherichia coli, GI1787029, Length=245, Percent_Identity=28.9795918367347, Blast_Score=115, Evalue=3e-27, Organism=Escherichia coli, GI87081791, Length=259, Percent_Identity=28.957528957529, Blast_Score=110, Evalue=1e-25, Organism=Escherichia coli, GI1789593, Length=252, Percent_Identity=30.1587301587302, Blast_Score=109, Evalue=2e-25, Organism=Escherichia coli, GI1789991, Length=243, Percent_Identity=30.8641975308642, Blast_Score=107, Evalue=6e-25, Organism=Escherichia coli, GI48995001, Length=224, Percent_Identity=31.25, Blast_Score=104, Evalue=6e-24, Organism=Escherichia coli, GI87081709, Length=262, Percent_Identity=29.0076335877863, Blast_Score=104, Evalue=7e-24, Organism=Escherichia coli, GI1788761, Length=255, Percent_Identity=30.1960784313725, Blast_Score=103, Evalue=2e-23, Organism=Escherichia coli, GI1786563, Length=264, Percent_Identity=30.6818181818182, Blast_Score=100, Evalue=1e-22, Organism=Escherichia coli, GI1788225, Length=246, Percent_Identity=28.4552845528455, Blast_Score=98, Evalue=5e-22, Organism=Escherichia coli, GI1788210, Length=254, Percent_Identity=28.3464566929134, Blast_Score=97, Evalue=1e-21, Organism=Escherichia coli, GI1786398, Length=234, Percent_Identity=30.3418803418803, Blast_Score=97, Evalue=1e-21, Organism=Escherichia coli, GI87082268, Length=252, Percent_Identity=30.1587301587302, Blast_Score=95, Evalue=5e-21, Organism=Escherichia coli, GI1788472, Length=234, Percent_Identity=24.3589743589744, Blast_Score=92, Evalue=3e-20, Organism=Escherichia coli, GI1790190, Length=233, Percent_Identity=25.3218884120172, Blast_Score=92, Evalue=4e-20, Organism=Escherichia coli, GI87081782, Length=259, Percent_Identity=26.2548262548263, Blast_Score=91, Evalue=9e-20, Organism=Escherichia coli, GI1789672, Length=246, Percent_Identity=23.9837398373984, Blast_Score=91, Evalue=1e-19, Organism=Escherichia coli, GI1790467, Length=257, Percent_Identity=27.6264591439689, Blast_Score=89, Evalue=2e-19, Organism=Escherichia coli, GI1786872, Length=246, Percent_Identity=25.2032520325203, Blast_Score=89, Evalue=4e-19, Organism=Escherichia coli, GI1788897, Length=233, Percent_Identity=27.4678111587983, Blast_Score=88, Evalue=7e-19, Organism=Escherichia coli, GI1787370, Length=264, Percent_Identity=26.5151515151515, Blast_Score=86, Evalue=2e-18, Organism=Escherichia coli, GI1787089, Length=243, Percent_Identity=25.1028806584362, Blast_Score=86, Evalue=3e-18, Organism=Escherichia coli, GI1787712, Length=246, Percent_Identity=25.609756097561, Blast_Score=86, Evalue=3e-18, Organism=Escherichia coli, GI1787792, Length=247, Percent_Identity=24.6963562753036, Blast_Score=85, Evalue=5e-18, Organism=Escherichia coli, GI1788644, Length=252, Percent_Identity=25, Blast_Score=85, Evalue=5e-18, Organism=Escherichia coli, GI1786253, Length=253, Percent_Identity=26.4822134387352, Blast_Score=85, Evalue=6e-18, Organism=Escherichia coli, GI1789962, Length=259, Percent_Identity=26.6409266409266, Blast_Score=84, Evalue=1e-17, Organism=Escherichia coli, GI1789032, Length=229, Percent_Identity=27.0742358078603, Blast_Score=83, Evalue=2e-17, Organism=Escherichia coli, GI1790525, Length=248, Percent_Identity=22.9838709677419, Blast_Score=82, Evalue=3e-17, Organism=Escherichia coli, GI1787105, Length=230, Percent_Identity=26.5217391304348, Blast_Score=81, Evalue=8e-17, Organism=Escherichia coli, GI87081834, Length=210, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-16, Organism=Escherichia coli, GI1790739, Length=240, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=3e-16, Organism=Escherichia coli, GI1787500, Length=235, Percent_Identity=28.936170212766, Blast_Score=79, Evalue=3e-16, Organism=Escherichia coli, GI1789586, Length=245, Percent_Identity=27.7551020408163, Blast_Score=79, Evalue=3e-16, Organism=Escherichia coli, GI1786703, Length=227, Percent_Identity=28.6343612334802, Blast_Score=78, Evalue=6e-16, Organism=Escherichia coli, GI48994943, Length=262, Percent_Identity=25.5725190839695, Blast_Score=78, Evalue=7e-16, Organism=Escherichia coli, GI87082267, Length=257, Percent_Identity=26.0700389105058, Blast_Score=77, Evalue=2e-15, Organism=Escherichia coli, GI1788450, Length=265, Percent_Identity=26.4150943396226, Blast_Score=76, Evalue=2e-15, Organism=Escherichia coli, GI1788506, Length=277, Percent_Identity=26.7148014440433, Blast_Score=76, Evalue=3e-15, Organism=Escherichia coli, GI1789873, Length=221, Percent_Identity=26.6968325791855, Blast_Score=75, Evalue=4e-15, Organism=Escherichia coli, GI1787164, Length=216, Percent_Identity=30.0925925925926, Blast_Score=75, Evalue=4e-15, Organism=Escherichia coli, GI1786803, Length=240, Percent_Identity=24.1666666666667, Blast_Score=75, Evalue=5e-15, Organism=Escherichia coli, GI1786345, Length=238, Percent_Identity=30.672268907563, Blast_Score=75, Evalue=6e-15, Organism=Escherichia coli, GI1786319, Length=222, Percent_Identity=27.4774774774775, Blast_Score=71, Evalue=1e-13, Organism=Escherichia coli, GI1790544, Length=252, Percent_Identity=25.7936507936508, Blast_Score=70, Evalue=1e-13, Organism=Escherichia coli, GI1787112, Length=248, Percent_Identity=30.241935483871, Blast_Score=69, Evalue=3e-13, Organism=Escherichia coli, GI48994997, Length=251, Percent_Identity=27.0916334661355, Blast_Score=68, Evalue=5e-13, Organism=Escherichia coli, GI1788053, Length=223, Percent_Identity=27.3542600896861, Blast_Score=67, Evalue=1e-12, Organism=Escherichia coli, GI1789891, Length=219, Percent_Identity=27.8538812785388, Blast_Score=67, Evalue=1e-12, Organism=Escherichia coli, GI1790534, Length=203, Percent_Identity=29.5566502463054, Blast_Score=66, Evalue=2e-12, Organism=Escherichia coli, GI1787759, Length=257, Percent_Identity=26.8482490272374, Blast_Score=64, Evalue=1e-11, Organism=Caenorhabditis elegans, GI115533608, Length=230, Percent_Identity=27.3913043478261, Blast_Score=83, Evalue=2e-16, Organism=Caenorhabditis elegans, GI71983523, Length=262, Percent_Identity=24.8091603053435, Blast_Score=74, Evalue=7e-14, Organism=Caenorhabditis elegans, GI71983516, Length=262, Percent_Identity=24.8091603053435, Blast_Score=74, Evalue=7e-14, Organism=Caenorhabditis elegans, GI71983510, Length=262, Percent_Identity=24.8091603053435, Blast_Score=74, Evalue=7e-14, Organism=Caenorhabditis elegans, GI17565586, Length=222, Percent_Identity=27.027027027027, Blast_Score=69, Evalue=3e-12, Organism=Drosophila melanogaster, GI221500365, Length=236, Percent_Identity=28.8135593220339, Blast_Score=82, Evalue=5e-16, Organism=Drosophila melanogaster, GI116007184, Length=236, Percent_Identity=28.8135593220339, Blast_Score=82, Evalue=5e-16, Organism=Drosophila melanogaster, GI221512771, Length=225, Percent_Identity=27.5555555555556, Blast_Score=76, Evalue=3e-14, Organism=Drosophila melanogaster, GI24666092, Length=244, Percent_Identity=26.6393442622951, Blast_Score=76, Evalue=3e-14, Organism=Drosophila melanogaster, GI24581191, Length=224, Percent_Identity=25.8928571428571, Blast_Score=72, Evalue=3e-13, Organism=Drosophila melanogaster, GI24581189, Length=224, Percent_Identity=25.8928571428571, Blast_Score=72, Evalue=3e-13, Organism=Drosophila melanogaster, GI24581193, Length=224, Percent_Identity=25.8928571428571, Blast_Score=72, Evalue=3e-13, Organism=Drosophila melanogaster, GI24581195, Length=224, Percent_Identity=25.8928571428571, Blast_Score=72, Evalue=3e-13, Organism=Drosophila melanogaster, GI24643674, Length=210, Percent_Identity=27.6190476190476, Blast_Score=69, Evalue=4e-12, Organism=Drosophila melanogaster, GI85726357, Length=231, Percent_Identity=29.004329004329, Blast_Score=65, Evalue=6e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003439 - InterPro: IPR017871 - InterPro: IPR003593 [H]
Pfam domain/function: PF00005 ABC_tran [H]
EC number: NA
Molecular weight: Translated: 29860; Mature: 29728
Theoretical pI: Translated: 10.20; Mature: 10.20
Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 0.7 %Met (Translated Protein) 1.4 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 0.4 %Met (Mature Protein) 1.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTGRPIPGPRPSTTLGPVLDVRDVTLRFGGLTSLDGVSLRQDRGSVLAVIGPNGAGKTSL CCCCCCCCCCCCCCCCCEECHHHHEEEECCCCCCCCCEEECCCCCEEEEECCCCCCHHHH FNCLTGAYRPQRGSARFWPADDGVELVGRSPHRVARLGVARTFQNIRLFPEMSAVENVQV HHHHHHCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCEEE GVEVRARTGALRGLLPTRAVRREERETLDRCHELLDFVGLGHRAERPAAALAYGEQRRLE CEEEEEHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCHHHHHH IARALGTSPSLLLLDEPAAGATAAERTDLVTLIRAIAGRGISVLLIEHDIRLVAAVARAI HHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHH VVLNFGRVIAAGRPDEVRADPAVVEAYLGEPDERRDGP HHHHCCCEEECCCCCCCCCCHHHHHHHHCCCHHCCCCC >Mature Secondary Structure TGRPIPGPRPSTTLGPVLDVRDVTLRFGGLTSLDGVSLRQDRGSVLAVIGPNGAGKTSL CCCCCCCCCCCCCCCCEECHHHHEEEECCCCCCCCCEEECCCCCEEEEECCCCCCHHHH FNCLTGAYRPQRGSARFWPADDGVELVGRSPHRVARLGVARTFQNIRLFPEMSAVENVQV HHHHHHCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCEEE GVEVRARTGALRGLLPTRAVRREERETLDRCHELLDFVGLGHRAERPAAALAYGEQRRLE CEEEEEHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCHHHHHH IARALGTSPSLLLLDEPAAGATAAERTDLVTLIRAIAGRGISVLLIEHDIRLVAAVARAI HHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHH VVLNFGRVIAAGRPDEVRADPAVVEAYLGEPDERRDGP HHHHCCCEEECCCCCCCCCCHHHHHHHHCCCHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]
Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]
General reaction: NA
Inhibitor: NA
Structure determination priority: 7.0
TargetDB status: NA
Availability: NA
References: 2120183; 10984043 [H]