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The map label for this gene is def

Identifier: 158313554

GI number: 158313554

Start: 2054801

End: 2055352

Strand: Direct

Name: def

Synonym: Franean1_1718

Alternate gene names: 158313554

Gene position: 2054801-2055352 (Clockwise)

Preceding gene: 158313553

Following gene: 158313555

Centisome position: 22.88

GC content: 68.3

Gene sequence:

>552_bases
GTGTCCGTCCGTGATATCCGCCTTCTCGGGGATCCGGTGCTGCGTACGGTCGCCGACCCGGTGGCGACCTTCGACCGGGA
GCTGCGCCGCCTCGTCGACGATCTCGCTGACACCATGCGCGACGCCGGCGGCGTCGGCCTCGCCGCGCCGCAGTTGGGGG
TGTCGCTGCGTATCTTCACGTACCTCGATGACTCGGACGAGGTCGGGCACCTGATCAACCCGGTCCTGGGGCCCTTCAGC
GAGGAGATGATGGACGGCGAGGAGGGCTGCCTGTCGTTGCCGGGGCTGGCATTCGACCTGCGCCGTCCGGAACGCGTCCT
CGCCGTCGGCCAGAACTCCCACGGTGACCCGGTCACGGTCGAGGGCAGTGGCATCCTGTCCCGATGCCTGCAACACGAGA
CCGATCACCTCGACGGGATCCTGTTCATCGACCGTCTCGACAAGGAGACGAAGCGGGCGGCCATGAAGGCGATCCGCGAG
GCGGAGTGGTCGAACGAGCCGAAGCCCGCGGTCAAGGTATCGCCGCACCCATTGTTCGGCCGCGGGCGCTGA

Upstream 100 bases:

>100_bases
CCGGTCCAATCCGGCGCCGGTTCCCGCGCGACCCGTAGCATGAAAGCGTCCGATCTCGCCGGACCGAGGTCGGTCAGGTG
ACGACATTCTGGGAGTGACC

Downstream 100 bases:

>100_bases
GTCGCCAGGGAAAGATCATCGCGTCGGCCGTCCTCGCCACGATGGCCACCGGAACGGTGATCGGCACGGCGGTAGGGTCG
ACGATCGGGTCCGCCGCCGG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MSVRDIRLLGDPVLRTVADPVATFDRELRRLVDDLADTMRDAGGVGLAAPQLGVSLRIFTYLDDSDEVGHLINPVLGPFS
EEMMDGEEGCLSLPGLAFDLRRPERVLAVGQNSHGDPVTVEGSGILSRCLQHETDHLDGILFIDRLDKETKRAAMKAIRE
AEWSNEPKPAVKVSPHPLFGRGR

Sequences:

>Translated_183_residues
MSVRDIRLLGDPVLRTVADPVATFDRELRRLVDDLADTMRDAGGVGLAAPQLGVSLRIFTYLDDSDEVGHLINPVLGPFS
EEMMDGEEGCLSLPGLAFDLRRPERVLAVGQNSHGDPVTVEGSGILSRCLQHETDHLDGILFIDRLDKETKRAAMKAIRE
AEWSNEPKPAVKVSPHPLFGRGR
>Mature_182_residues
SVRDIRLLGDPVLRTVADPVATFDRELRRLVDDLADTMRDAGGVGLAAPQLGVSLRIFTYLDDSDEVGHLINPVLGPFSE
EMMDGEEGCLSLPGLAFDLRRPERVLAVGQNSHGDPVTVEGSGILSRCLQHETDHLDGILFIDRLDKETKRAAMKAIREA
EWSNEPKPAVKVSPHPLFGRGR

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=164, Percent_Identity=34.1463414634146, Blast_Score=81, Evalue=4e-16,
Organism=Escherichia coli, GI1789682, Length=148, Percent_Identity=41.2162162162162, Blast_Score=94, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24645728, Length=168, Percent_Identity=32.1428571428571, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24645726, Length=163, Percent_Identity=32.5153374233129, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_FRASN (A8LE21)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001506062.1
- ProteinModelPortal:   A8LE21
- SMR:   A8LE21
- GeneID:   5670120
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_1718
- HOGENOM:   HBG665227
- OMA:   AIREAEW
- BioCyc:   FSP1855:FRANEAN1_1718-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20091; Mature: 19960

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRDIRLLGDPVLRTVADPVATFDRELRRLVDDLADTMRDAGGVGLAAPQLGVSLRIFT
CCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
YLDDSDEVGHLINPVLGPFSEEMMDGEEGCLSLPGLAFDLRRPERVLAVGQNSHGDPVTV
EECCCCHHHHHHHHHCCCHHHHHHCCCCCCEECCCCEEECCCCCEEEEECCCCCCCEEEE
EGSGILSRCLQHETDHLDGILFIDRLDKETKRAAMKAIREAEWSNEPKPAVKVSPHPLFG
ECCHHHHHHHHHCCCCCCCEEEHHHHCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCC
RGR
CCC
>Mature Secondary Structure 
SVRDIRLLGDPVLRTVADPVATFDRELRRLVDDLADTMRDAGGVGLAAPQLGVSLRIFT
CCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
YLDDSDEVGHLINPVLGPFSEEMMDGEEGCLSLPGLAFDLRRPERVLAVGQNSHGDPVTV
EECCCCHHHHHHHHHCCCHHHHHHCCCCCCEECCCCEEECCCCCEEEEECCCCCCCEEEE
EGSGILSRCLQHETDHLDGILFIDRLDKETKRAAMKAIREAEWSNEPKPAVKVSPHPLFG
ECCHHHHHHHHHCCCCCCCEEEHHHHCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCC
RGR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA