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The map label for this gene is fadH [C]

Identifier: 157963883

GI number: 157963883

Start: 4950560

End: 4951660

Strand: Reverse

Name: fadH [C]

Synonym: Spea_4072

Alternate gene names: 157963883

Gene position: 4951660-4950560 (Counterclockwise)

Preceding gene: 157963885

Following gene: 157963882

Centisome position: 95.69

GC content: 47.87

Gene sequence:

>1101_bases
GTGAGCACTTTGTTTTCACCTAGCCGTATTGGCAAGATGACACTGAAAAACCGCTTTGTGAGAAGCGCGACCTGGGAAAA
TATGGCGACTGAAATTGGCCATATGACCGACAAGTTGTACTCTGTGTATGAAGAGCTGGCTAAAGGTGAGGTGGGGCTGA
TTGTCACGGGTTACGCTAACATCGTTGCCGAAGAAAAGCCTAACGCTGGCATGATGGGTATGTATGACGATTCGTTTATT
GCTGAATACCAGAAGCTGACAGACTTGGTGCATGGCTATGACTCGAAAATCGTGATGCAGCTGGCTTATGGTGGCACTAA
GACCACTTACAACGTCGGGGAGCGAGTGATCTTTGCCCCGAGTGCTATTGCTGAGCGTGGTACTCAAACCATGGGGAAGG
CGATGACTAAGGCTGAGATCGATTACATCGTAAAGGCGTTTGCCGAGGCGAGTCGCCGCGCACAAGAGTCAGGCTTCGAT
GGCGTTGAGATCCATGCGGCGCACACTTATCTGATTAATCAGTTTCTAAGCCCATACTACAATCAGCGTGAAGATGAATA
TGGCGGTAGCCTTGAGAACCGTATGCGCTTCTTGCTGGAGATCTACGCAGAGATCCGCAAACTTGTTGGTGATGATTACC
CTATTTTAGTGAAGCTGACCGCCTCTGAGTTTTTTGATGGCGGCTTAACTTTTGCTGACACCCGCAGCATTTGTAAAACG
CTGGAGTCGATTGGCGTCGATGCGATTATTATCTCTGGCAACATCCATGGTAATGCCAATACTGTGGTGGGTGAACAATT
TGATGGTTATACCATTCAAAGTGAAGGTTACTTCCGTGAATATGGCGCCGTGGTCAGTCAGGAAGTTAATCTGCCAATTA
TTACCGTAGGCGGATTAGCTGATATCTGCGCCATTGAAGAACTTGCCAATAGCACTAATATTCAGTTTTTTGCACTGTCA
CGCCCATTACTGGCCGAACCGCAGCTTATTAAGCGCTGGAAGGAGGGCAATGCTGCTGAAGTGGATTGTGAAAGATGTTC
TAAGTGCCGTACTAAGCGCGGTAACTTCTGTGTGGTATATAAGAAGCGTAACGCCGCTTAA

Upstream 100 bases:

>100_bases
GACTGACTACAGCCATGCTTTTCTAAAGTGTGGCTGTTGTGTTTTTACTGTTTGCTATTTTTGTTGGTTAGTACTGAATA
AGATTTTAAGAGGATTGCAC

Downstream 100 bases:

>100_bases
GTTTTATTACTAGCAACAATGAGGCGCAGTAATCTTGGGCCTCATTGTTATGCTTTCTTAATACTGATTAGTATTTGATT
TGTATGAGTCGGTCCCGATT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MSTLFSPSRIGKMTLKNRFVRSATWENMATEIGHMTDKLYSVYEELAKGEVGLIVTGYANIVAEEKPNAGMMGMYDDSFI
AEYQKLTDLVHGYDSKIVMQLAYGGTKTTYNVGERVIFAPSAIAERGTQTMGKAMTKAEIDYIVKAFAEASRRAQESGFD
GVEIHAAHTYLINQFLSPYYNQREDEYGGSLENRMRFLLEIYAEIRKLVGDDYPILVKLTASEFFDGGLTFADTRSICKT
LESIGVDAIIISGNIHGNANTVVGEQFDGYTIQSEGYFREYGAVVSQEVNLPIITVGGLADICAIEELANSTNIQFFALS
RPLLAEPQLIKRWKEGNAAEVDCERCSKCRTKRGNFCVVYKKRNAA

Sequences:

>Translated_366_residues
MSTLFSPSRIGKMTLKNRFVRSATWENMATEIGHMTDKLYSVYEELAKGEVGLIVTGYANIVAEEKPNAGMMGMYDDSFI
AEYQKLTDLVHGYDSKIVMQLAYGGTKTTYNVGERVIFAPSAIAERGTQTMGKAMTKAEIDYIVKAFAEASRRAQESGFD
GVEIHAAHTYLINQFLSPYYNQREDEYGGSLENRMRFLLEIYAEIRKLVGDDYPILVKLTASEFFDGGLTFADTRSICKT
LESIGVDAIIISGNIHGNANTVVGEQFDGYTIQSEGYFREYGAVVSQEVNLPIITVGGLADICAIEELANSTNIQFFALS
RPLLAEPQLIKRWKEGNAAEVDCERCSKCRTKRGNFCVVYKKRNAA
>Mature_365_residues
STLFSPSRIGKMTLKNRFVRSATWENMATEIGHMTDKLYSVYEELAKGEVGLIVTGYANIVAEEKPNAGMMGMYDDSFIA
EYQKLTDLVHGYDSKIVMQLAYGGTKTTYNVGERVIFAPSAIAERGTQTMGKAMTKAEIDYIVKAFAEASRRAQESGFDG
VEIHAAHTYLINQFLSPYYNQREDEYGGSLENRMRFLLEIYAEIRKLVGDDYPILVKLTASEFFDGGLTFADTRSICKTL
ESIGVDAIIISGNIHGNANTVVGEQFDGYTIQSEGYFREYGAVVSQEVNLPIITVGGLADICAIEELANSTNIQFFALSR
PLLAEPQLIKRWKEGNAAEVDCERCSKCRTKRGNFCVVYKKRNAA

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=351, Percent_Identity=28.4900284900285, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787939, Length=363, Percent_Identity=30.0275482093664, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17540738, Length=351, Percent_Identity=30.1994301994302, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17565138, Length=336, Percent_Identity=29.4642857142857, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI72001454, Length=347, Percent_Identity=30.5475504322767, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17559802, Length=348, Percent_Identity=30.1724137931034, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17564188, Length=347, Percent_Identity=28.8184438040346, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17559804, Length=349, Percent_Identity=30.3724928366762, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17566914, Length=305, Percent_Identity=29.1803278688525, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17566916, Length=353, Percent_Identity=25.4957507082153, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=225, Percent_Identity=28, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321973, Length=225, Percent_Identity=28.8888888888889, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 40712; Mature: 40581

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLFSPSRIGKMTLKNRFVRSATWENMATEIGHMTDKLYSVYEELAKGEVGLIVTGYAN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHH
IVAEEKPNAGMMGMYDDSFIAEYQKLTDLVHGYDSKIVMQLAYGGTKTTYNVGERVIFAP
HEECCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEECCCEEEECC
SAIAERGTQTMGKAMTKAEIDYIVKAFAEASRRAQESGFDGVEIHAAHTYLINQFLSPYY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHH
NQREDEYGGSLENRMRFLLEIYAEIRKLVGDDYPILVKLTASEFFDGGLTFADTRSICKT
CCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCHHHHHHHHHH
LESIGVDAIIISGNIHGNANTVVGEQFDGYTIQSEGYFREYGAVVSQEVNLPIITVGGLA
HHHCCCCEEEEECCCCCCCCEEECCCCCCEEECCCCCHHHHHHHHHCCCCCCEEEECCHH
DICAIEELANSTNIQFFALSRPLLAEPQLIKRWKEGNAAEVDCERCSKCRTKRGNFCVVY
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEE
KKRNAA
ECCCCC
>Mature Secondary Structure 
STLFSPSRIGKMTLKNRFVRSATWENMATEIGHMTDKLYSVYEELAKGEVGLIVTGYAN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHH
IVAEEKPNAGMMGMYDDSFIAEYQKLTDLVHGYDSKIVMQLAYGGTKTTYNVGERVIFAP
HEECCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEECCCEEEECC
SAIAERGTQTMGKAMTKAEIDYIVKAFAEASRRAQESGFDGVEIHAAHTYLINQFLSPYY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHH
NQREDEYGGSLENRMRFLLEIYAEIRKLVGDDYPILVKLTASEFFDGGLTFADTRSICKT
CCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCHHHHHHHHHH
LESIGVDAIIISGNIHGNANTVVGEQFDGYTIQSEGYFREYGAVVSQEVNLPIITVGGLA
HHHCCCCEEEEECCCCCCCCEEECCCCCCEEECCCCCHHHHHHHHHCCCCCCEEEECCHH
DICAIEELANSTNIQFFALSRPLLAEPQLIKRWKEGNAAEVDCERCSKCRTKRGNFCVVY
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEE
KKRNAA
ECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]