Click here to switch to the map view.

The map label for this gene is hipO [H]

Identifier: 157963885

GI number: 157963885

Start: 4954050

End: 4955204

Strand: Reverse

Name: hipO [H]

Synonym: Spea_4074

Alternate gene names: 157963885

Gene position: 4955204-4954050 (Counterclockwise)

Preceding gene: 157963886

Following gene: 157963883

Centisome position: 95.76

GC content: 48.4

Gene sequence:

>1155_bases
ATGAATATCGAACAGAAAGTTATTGAGTGGCGTCATCACATTCACCAGCACCCAGAGTTTGGTACCCAAGAGCACGAGAC
TGCAGCATTTGTAGCAAGCAAACTAGAAGAGTTTGGTATCGAAGTGCACACTGGCATTGGTGGCACTGGCGTTGTGGGTA
TCTTAAAGTGTGGTGATAGCGATCGTTCAATCGGCCTACGTGCCGATATGGATGCACTGCATATTCATGAAGAAAACACC
TTCGATTACGCCTCAGTAAACGAAGGCGCAATGCACGCTTGTGGTCACGATGGTCACACGGCAATGCTACTTGCTGCAGC
CCATGAGCTAGCACAAACCAAGAACTTCGACGGTACTGTTTACTTCATCTTCCAACCTGATGAAGAGCGTGGCACAGGTG
CTAAGGCTATGATTGCCGACGGCCTATTCACTCGTTGGAAAATCGACGGCGTGTACGCAATGCACAACCTACCGGGTATT
GAAGCAGGTCACTTCGTGACGCGTCCACACTCTGTTATGGCAAGTGAAAGCAGCTTCGAAATCGAAGTACTTGCAACGGG
CGGACACGCGGCAATGCCACACATGGGTACCGATCCGATCGTGGTTGGCGCGCAAATCGTGACTGCAATCCAAACTATCG
TATCGCGTAACCTAAGCGCGATTGACGAAACAGCAGTTATCTCGGTAACAGAATTCGCTACTAACGGTACGGTTAACGTT
ATCCCAACTAAAGTGACTATCACTGGTGATACCCGTAGCTTCACCGATATTGCATTAAACAAGATTGAAAAAGCCCTTGA
GCGTGTAGTCGCGGGTCAGTGTATGTCAGCTGGCGTTGATTACACTTACAAGTTTAACAACAGCTTCCTATCGACTATCA
ACACACCAGCTGAAACGGCTTATGCGGTTCGCGCTGCTGAGACTGTTGTTGGCGAAGATAAAGTTAACGGCAGCTGCCAG
CCATTCACTATCAGTGAAGACTTCTCGTTTATGCTACGTGAAGCACCAGGTTGTTACATCTTAGTGGGTAACGGCGCTGG
CTGTGGTTGTGGTAGCGTGGCGCTACACAAGCCAACTTATGACTTCAATGACAATATCTTAGTATCTGGTCTGAGATACT
GGCAGACTCTTGTTGAACAGTGTTTGGCTAAGTAG

Upstream 100 bases:

>100_bases
CCTTGCCGCAGCCAGTAACTGAATAATTCAGTTAGCAAATTGAATCAATTTTTATCACCGGTGACCTAGTCACCGGTTTG
TTGTTATAAGAGAAAAGCAT

Downstream 100 bases:

>100_bases
TTCGTTATAAACTATTTGCTATAGTTAGCAGTGTTTAGATTAAGGCTATGAGCTTAGGTTCATAGCCTTTTTTGTTACTT
GGTTTTGCGCCAAGCGGGCG

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MNIEQKVIEWRHHIHQHPEFGTQEHETAAFVASKLEEFGIEVHTGIGGTGVVGILKCGDSDRSIGLRADMDALHIHEENT
FDYASVNEGAMHACGHDGHTAMLLAAAHELAQTKNFDGTVYFIFQPDEERGTGAKAMIADGLFTRWKIDGVYAMHNLPGI
EAGHFVTRPHSVMASESSFEIEVLATGGHAAMPHMGTDPIVVGAQIVTAIQTIVSRNLSAIDETAVISVTEFATNGTVNV
IPTKVTITGDTRSFTDIALNKIEKALERVVAGQCMSAGVDYTYKFNNSFLSTINTPAETAYAVRAAETVVGEDKVNGSCQ
PFTISEDFSFMLREAPGCYILVGNGAGCGCGSVALHKPTYDFNDNILVSGLRYWQTLVEQCLAK

Sequences:

>Translated_384_residues
MNIEQKVIEWRHHIHQHPEFGTQEHETAAFVASKLEEFGIEVHTGIGGTGVVGILKCGDSDRSIGLRADMDALHIHEENT
FDYASVNEGAMHACGHDGHTAMLLAAAHELAQTKNFDGTVYFIFQPDEERGTGAKAMIADGLFTRWKIDGVYAMHNLPGI
EAGHFVTRPHSVMASESSFEIEVLATGGHAAMPHMGTDPIVVGAQIVTAIQTIVSRNLSAIDETAVISVTEFATNGTVNV
IPTKVTITGDTRSFTDIALNKIEKALERVVAGQCMSAGVDYTYKFNNSFLSTINTPAETAYAVRAAETVVGEDKVNGSCQ
PFTISEDFSFMLREAPGCYILVGNGAGCGCGSVALHKPTYDFNDNILVSGLRYWQTLVEQCLAK
>Mature_384_residues
MNIEQKVIEWRHHIHQHPEFGTQEHETAAFVASKLEEFGIEVHTGIGGTGVVGILKCGDSDRSIGLRADMDALHIHEENT
FDYASVNEGAMHACGHDGHTAMLLAAAHELAQTKNFDGTVYFIFQPDEERGTGAKAMIADGLFTRWKIDGVYAMHNLPGI
EAGHFVTRPHSVMASESSFEIEVLATGGHAAMPHMGTDPIVVGAQIVTAIQTIVSRNLSAIDETAVISVTEFATNGTVNV
IPTKVTITGDTRSFTDIALNKIEKALERVVAGQCMSAGVDYTYKFNNSFLSTINTPAETAYAVRAAETVVGEDKVNGSCQ
PFTISEDFSFMLREAPGCYILVGNGAGCGCGSVALHKPTYDFNDNILVSGLRYWQTLVEQCLAK

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=419, Percent_Identity=25.2983293556086, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41456; Mature: 41456

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEQKVIEWRHHIHQHPEFGTQEHETAAFVASKLEEFGIEVHTGIGGTGVVGILKCGDS
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECCC
DRSIGLRADMDALHIHEENTFDYASVNEGAMHACGHDGHTAMLLAAAHELAQTKNFDGTV
CCCCCEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEE
YFIFQPDEERGTGAKAMIADGLFTRWKIDGVYAMHNLPGIEAGHFVTRPHSVMASESSFE
EEEECCCCCCCCCCCEEHHCCCEEEEEECCEEEECCCCCCCCCCEEECCCHHEECCCCEE
IEVLATGGHAAMPHMGTDPIVVGAQIVTAIQTIVSRNLSAIDETAVISVTEFATNGTVNV
EEEEEECCCEECCCCCCCCEEEHHHHHHHHHHHHHCCCHHCCCEEEEEEEEECCCCEEEE
IPTKVTITGDTRSFTDIALNKIEKALERVVAGQCMSAGVDYTYKFNNSFLSTINTPAETA
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEECCCCCHHHH
YAVRAAETVVGEDKVNGSCQPFTISEDFSFMLREAPGCYILVGNGAGCGCGSVALHKPTY
HHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHCCCCEEEEEECCCCCCCCCEEEECCCC
DFNDNILVSGLRYWQTLVEQCLAK
CCCCCEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIEQKVIEWRHHIHQHPEFGTQEHETAAFVASKLEEFGIEVHTGIGGTGVVGILKCGDS
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECCC
DRSIGLRADMDALHIHEENTFDYASVNEGAMHACGHDGHTAMLLAAAHELAQTKNFDGTV
CCCCCEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEE
YFIFQPDEERGTGAKAMIADGLFTRWKIDGVYAMHNLPGIEAGHFVTRPHSVMASESSFE
EEEECCCCCCCCCCCEEHHCCCEEEEEECCEEEECCCCCCCCCCEEECCCHHEECCCCEE
IEVLATGGHAAMPHMGTDPIVVGAQIVTAIQTIVSRNLSAIDETAVISVTEFATNGTVNV
EEEEEECCCEECCCCCCCCEEEHHHHHHHHHHHHHCCCHHCCCEEEEEEEEECCCCEEEE
IPTKVTITGDTRSFTDIALNKIEKALERVVAGQCMSAGVDYTYKFNNSFLSTINTPAETA
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEECCCCCHHHH
YAVRAAETVVGEDKVNGSCQPFTISEDFSFMLREAPGCYILVGNGAGCGCGSVALHKPTY
HHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHCCCCEEEEEECCCCCCCCCEEEECCCC
DFNDNILVSGLRYWQTLVEQCLAK
CCCCCEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]