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The map label for this gene is nrfB [H]

Identifier: 157963778

GI number: 157963778

Start: 4820971

End: 4821441

Strand: Reverse

Name: nrfB [H]

Synonym: Spea_3967

Alternate gene names: 157963778

Gene position: 4821441-4820971 (Counterclockwise)

Preceding gene: 157963779

Following gene: 157963777

Centisome position: 93.18

GC content: 42.25

Gene sequence:

>471_bases
ATGTCCAGCTTATTTAAATATTCAGTTTCCTTGTTGATGAGCATTTTACTTGTTGTTGCCGCTTCGGTGATATCTATCAA
GGCTGTTGCCGCAGAATTTAAAATTAATGAAAATAAACAATGCATTATGTGCCATAAGCGCAATGGAAAAATGCTCGGCG
TACATGCCAATGACGCCTTAGATATCAGTTGTCAGGATTGTCATGGTGAAAAAGAGGGACATCCAAGAAAGGCATCCAAC
TTAATCGGCTTTAGTAATAAGTCGGCCTCAGATGTTAATACGCAAACTCAAAGCTGTTTGAAGTGTCACGACCATAGCGT
ATTAGCTGAACGTGATTGGAGCCATGACGCTCACTCTAACAAGGTGAATTGTGCTAGCTGCCATCTTTTACATCCCAACA
CAGATCCTGTGCTAGGCGTATCTGCAAAAGAGCGTAGTGAACTGTGTAGCAGTTGCCACGTGGCCAAGTAA

Upstream 100 bases:

>100_bases
AGCTCGTTAGTCGCATAACATCAATGGGTGTTGCAACATTAGTTGTAACACCTCATCCCCAACTCTCTTTATTTATCACT
TAGTAATGTGAGGACACCCC

Downstream 100 bases:

>100_bases
TTAGGGGAATCTATATGTCTAACTCAAGAAGATTGTTTTTAAAAGGTGCCTGTGCAGCGGCGGTTGGGGCTTCCGGCTTC
ACCATCGCAACGGCCAAAGG

Product: formate-dependent nitrite reductase

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MSSLFKYSVSLLMSILLVVAASVISIKAVAAEFKINENKQCIMCHKRNGKMLGVHANDALDISCQDCHGEKEGHPRKASN
LIGFSNKSASDVNTQTQSCLKCHDHSVLAERDWSHDAHSNKVNCASCHLLHPNTDPVLGVSAKERSELCSSCHVAK

Sequences:

>Translated_156_residues
MSSLFKYSVSLLMSILLVVAASVISIKAVAAEFKINENKQCIMCHKRNGKMLGVHANDALDISCQDCHGEKEGHPRKASN
LIGFSNKSASDVNTQTQSCLKCHDHSVLAERDWSHDAHSNKVNCASCHLLHPNTDPVLGVSAKERSELCSSCHVAK
>Mature_155_residues
SSLFKYSVSLLMSILLVVAASVISIKAVAAEFKINENKQCIMCHKRNGKMLGVHANDALDISCQDCHGEKEGHPRKASNL
IGFSNKSASDVNTQTQSCLKCHDHSVLAERDWSHDAHSNKVNCASCHLLHPNTDPVLGVSAKERSELCSSCHVAK

Specific function: Plays a role in nitrite reduction [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082366, Length=124, Percent_Identity=35.4838709677419, Blast_Score=78, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017564
- InterPro:   IPR011031 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 16970; Mature: 16838

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
9.0 %Cys+Met (Translated Protein)
6.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLFKYSVSLLMSILLVVAASVISIKAVAAEFKINENKQCIMCHKRNGKMLGVHANDAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEECCCCCEEEEECCCEE
DISCQDCHGEKEGHPRKASNLIGFSNKSASDVNTQTQSCLKCHDHSVLAERDWSHDAHSN
EEEECCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCC
KVNCASCHLLHPNTDPVLGVSAKERSELCSSCHVAK
CCCEEEEEEECCCCCCEEECCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSLFKYSVSLLMSILLVVAASVISIKAVAAEFKINENKQCIMCHKRNGKMLGVHANDAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEECCCCCEEEEECCCEE
DISCQDCHGEKEGHPRKASNLIGFSNKSASDVNTQTQSCLKCHDHSVLAERDWSHDAHSN
EEEECCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCC
KVNCASCHLLHPNTDPVLGVSAKERSELCSSCHVAK
CCCEEEEEEECCCCCCEEECCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]