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The map label for this gene is pcl [H]

Identifier: 157963702

GI number: 157963702

Start: 4736702

End: 4738081

Strand: Direct

Name: pcl [H]

Synonym: Spea_3891

Alternate gene names: 157963702

Gene position: 4736702-4738081 (Clockwise)

Preceding gene: 157963701

Following gene: 157963703

Centisome position: 91.54

GC content: 51.09

Gene sequence:

>1380_bases
ATGACGAATCTACTGAAATCTTGGCTATGCCAGGGACCTACTGCTCAGCAGCTAATTAGCTTCAATCACCACGACATTGT
GACAGGTTCGGTATTTACCGCCCAAGTCGCCCAGCTAAAGCAACAACTCAGCGAATCGGACGCCAAGCGTTGGTTACTGA
GCTGCGATAGTAGCGACCTGTTTGCCGTAGGTATATGTGCAGGCTTGCTCGCGGGCAAACAGATTATCCTGCCGGCTAAC
GCTCAGGCTGGCACCTTAAGCGAACTCACCCATGAGTTTGATGGCGTGCTTTCCAATATGCCAATTTGCGAAGGCAAGGC
CTTCTTGCGCTTAGATAAAGATCATAATCCACCAAGCAGCAACTGGCCCGAGTCACAGGAATGGGGACAGCTAGTCCTCT
TTACCTCAGGCAGCAGCGGCAAGCCTAAGCGAGTCGTTAAGACGATTGAACAGCTCGATGCCGAGGTAAGCGTACTTGAG
CATACTTTTGCCCAGCACCTGCCCCACTGCAGCGTGATATCGACCGTATCCCACCAGCATATTTATGGCTTACTGTTTAA
GATCCTTTGGCCGTTAGCCGCAAGCCGCCCCTTTTTAAGCGATCTGGTGGAATACCCTGAAACCCTAAGCTACTACGCCA
ACTTGTTCCCCAATTTATGCTTAGTGAGCAGCCCGGCTCAACTCTCTCGACTCCCTGATGCACTCGATCATGAACGACAA
CTGCGTGCGCCAAGCTTAGTCTTTAGCTCGGGCGGACCATTAAGTTATGAAGCCTCTAAGGGAGTAGCCAATTGTTACGG
CCAAGCCCCGATTGAGGTGTTTGGCAGTACCGAGACTGGCGGTATAGCTTATCGCCGCCAAGATACCGCCAACTCTAGCT
GGCAAAGCTTTGCCCCCATTGAGATTATGCAAGATGAGGCCGACGGTGCACTGCTGCTAAAATCCCCTTACCTGCCAGAC
AGTGAGTGGCTTAAGTGTGATGATAAAATTGCCCTGCAGGCCGATGGCAAGTTTACTCTTCAAGGGCGCTTAGACCGTAT
CATTAAAGTCGAAGAAAAACGCCTGTCATTGGTGCAGATGGAGTCTCTGCTAGAGAGTCACCCTCATGTCGCAAAAGCTG
CATTAGTCATGCTTGAACAACCTAGAGTGCAACTTGGCGCCGCCATTGAGCTTTCAGCTGAGGGATTACTGCAACTCGAA
GCAAACGGCAAGCTGGCACTCAATAACCAACTCAAGAAGCACCTGTTGTCGCAGTTTGAGCGCGTCACCCTTCCTAGGCG
CTGGCGCTATACAGAGCAGTTACCCGTGAACCCACAAGGTAAACGAGTGCAGGCCGACATGTTAGCCCTGTTTGATATCG
ATAAGTTTAAGAGCAAATGA

Upstream 100 bases:

>100_bases
TCGAAACATGGACACTTTATAACGGGCTGATTGCCTACTTGCTTATCGGGTCGCTACTGGGTGGCGAATGGTTATACAGA
ACATTCTGGTTGAAAAAATC

Downstream 100 bases:

>100_bases
TTAGATCTAAATTGCCCCCTATCATCGACTGTAAAGATGACCAAGATTCAGTAACTTGGACCATAGAAATTGCCGCCGAC
CTTGATTTTTTCTCGGGGCA

Product: hypothetical protein

Products: NA

Alternate protein names: 4CL; 4-coumaroyl-CoA synthase [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MTNLLKSWLCQGPTAQQLISFNHHDIVTGSVFTAQVAQLKQQLSESDAKRWLLSCDSSDLFAVGICAGLLAGKQIILPAN
AQAGTLSELTHEFDGVLSNMPICEGKAFLRLDKDHNPPSSNWPESQEWGQLVLFTSGSSGKPKRVVKTIEQLDAEVSVLE
HTFAQHLPHCSVISTVSHQHIYGLLFKILWPLAASRPFLSDLVEYPETLSYYANLFPNLCLVSSPAQLSRLPDALDHERQ
LRAPSLVFSSGGPLSYEASKGVANCYGQAPIEVFGSTETGGIAYRRQDTANSSWQSFAPIEIMQDEADGALLLKSPYLPD
SEWLKCDDKIALQADGKFTLQGRLDRIIKVEEKRLSLVQMESLLESHPHVAKAALVMLEQPRVQLGAAIELSAEGLLQLE
ANGKLALNNQLKKHLLSQFERVTLPRRWRYTEQLPVNPQGKRVQADMLALFDIDKFKSK

Sequences:

>Translated_459_residues
MTNLLKSWLCQGPTAQQLISFNHHDIVTGSVFTAQVAQLKQQLSESDAKRWLLSCDSSDLFAVGICAGLLAGKQIILPAN
AQAGTLSELTHEFDGVLSNMPICEGKAFLRLDKDHNPPSSNWPESQEWGQLVLFTSGSSGKPKRVVKTIEQLDAEVSVLE
HTFAQHLPHCSVISTVSHQHIYGLLFKILWPLAASRPFLSDLVEYPETLSYYANLFPNLCLVSSPAQLSRLPDALDHERQ
LRAPSLVFSSGGPLSYEASKGVANCYGQAPIEVFGSTETGGIAYRRQDTANSSWQSFAPIEIMQDEADGALLLKSPYLPD
SEWLKCDDKIALQADGKFTLQGRLDRIIKVEEKRLSLVQMESLLESHPHVAKAALVMLEQPRVQLGAAIELSAEGLLQLE
ANGKLALNNQLKKHLLSQFERVTLPRRWRYTEQLPVNPQGKRVQADMLALFDIDKFKSK
>Mature_458_residues
TNLLKSWLCQGPTAQQLISFNHHDIVTGSVFTAQVAQLKQQLSESDAKRWLLSCDSSDLFAVGICAGLLAGKQIILPANA
QAGTLSELTHEFDGVLSNMPICEGKAFLRLDKDHNPPSSNWPESQEWGQLVLFTSGSSGKPKRVVKTIEQLDAEVSVLEH
TFAQHLPHCSVISTVSHQHIYGLLFKILWPLAASRPFLSDLVEYPETLSYYANLFPNLCLVSSPAQLSRLPDALDHERQL
RAPSLVFSSGGPLSYEASKGVANCYGQAPIEVFGSTETGGIAYRRQDTANSSWQSFAPIEIMQDEADGALLLKSPYLPDS
EWLKCDDKIALQADGKFTLQGRLDRIIKVEEKRLSLVQMESLLESHPHVAKAALVMLEQPRVQLGAAIELSAEGLLQLEA
NGKLALNNQLKKHLLSQFERVTLPRRWRYTEQLPVNPQGKRVQADMLALFDIDKFKSK

Specific function: Converts p-coumaric acid into p-coumaryl CoA. This is necessary for the activation of the photoactive yellow protein (PYP) chromophore [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012743
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.12 [H]

Molecular weight: Translated: 50850; Mature: 50718

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLLKSWLCQGPTAQQLISFNHHDIVTGSVFTAQVAQLKQQLSESDAKRWLLSCDSSDL
CCHHHHHHHCCCCCHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
FAVGICAGLLAGKQIILPANAQAGTLSELTHEFDGVLSNMPICEGKAFLRLDKDHNPPSS
HHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCC
NWPESQEWGQLVLFTSGSSGKPKRVVKTIEQLDAEVSVLEHTFAQHLPHCSVISTVSHQH
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IYGLLFKILWPLAASRPFLSDLVEYPETLSYYANLFPNLCLVSSPAQLSRLPDALDHERQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHCCHHHCHHHH
LRAPSLVFSSGGPLSYEASKGVANCYGQAPIEVFGSTETGGIAYRRQDTANSSWQSFAPI
CCCCCEEEECCCCCCCCCCCCHHHHCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCC
EIMQDEADGALLLKSPYLPDSEWLKCDDKIALQADGKFTLQGRLDRIIKVEEKRLSLVQM
EEEECCCCCEEEEECCCCCCHHHEECCCCEEEEECCCEEECCHHHHHHHHHHHHHHHHHH
ESLLESHPHVAKAALVMLEQPRVQLGAAIELSAEGLLQLEANGKLALNNQLKKHLLSQFE
HHHHHCCCHHHHHHHHHHCCCCHHHCCEEEECCCCEEEEECCCCEEEHHHHHHHHHHHHH
RVTLPRRWRYTEQLPVNPQGKRVQADMLALFDIDKFKSK
HCCCCHHCCCCCCCCCCCCCCEEHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
TNLLKSWLCQGPTAQQLISFNHHDIVTGSVFTAQVAQLKQQLSESDAKRWLLSCDSSDL
CHHHHHHHCCCCCHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
FAVGICAGLLAGKQIILPANAQAGTLSELTHEFDGVLSNMPICEGKAFLRLDKDHNPPSS
HHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCC
NWPESQEWGQLVLFTSGSSGKPKRVVKTIEQLDAEVSVLEHTFAQHLPHCSVISTVSHQH
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IYGLLFKILWPLAASRPFLSDLVEYPETLSYYANLFPNLCLVSSPAQLSRLPDALDHERQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHCCHHHCHHHH
LRAPSLVFSSGGPLSYEASKGVANCYGQAPIEVFGSTETGGIAYRRQDTANSSWQSFAPI
CCCCCEEEECCCCCCCCCCCCHHHHCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCC
EIMQDEADGALLLKSPYLPDSEWLKCDDKIALQADGKFTLQGRLDRIIKVEEKRLSLVQM
EEEECCCCCEEEEECCCCCCHHHEECCCCEEEEECCCEEECCHHHHHHHHHHHHHHHHHH
ESLLESHPHVAKAALVMLEQPRVQLGAAIELSAEGLLQLEANGKLALNNQLKKHLLSQFE
HHHHHCCCHHHHHHHHHHCCCCHHHCCEEEECCCCEEEEECCCCEEEHHHHHHHHHHHHH
RVTLPRRWRYTEQLPVNPQGKRVQADMLALFDIDKFKSK
HCCCCHHCCCCCCCCCCCCCCEEHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA