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The map label for this gene is gcvA [H]

Identifier: 157962802

GI number: 157962802

Start: 3656252

End: 3657163

Strand: Reverse

Name: gcvA [H]

Synonym: Spea_2984

Alternate gene names: 157962802

Gene position: 3657163-3656252 (Counterclockwise)

Preceding gene: 157962804

Following gene: 157962801

Centisome position: 70.68

GC content: 45.29

Gene sequence:

>912_bases
ATGGCAAGACGATTACCTCCCCTAAATGCAGTAAAGGCATTTGAGGCTGCAGCTAGGCATTTAAGCTTCACTCGAGCAGC
TGAAGAATTGTTTGTTACTCAGGCTGCTGTGAGTCATCAAATTAAAGCATTAGAAGAGTACCTTGGGTTAAAATTATTTC
GTCGTAAGAACCGTTCATTACTGTTAACCGAGGAAGGGCAAAGCTACTTTCTCGATATAAAAGATATCTTCACTCAGCTT
GCCGATGCGACAGACAGATTATTAGCGAGAAGTGCCATAGGCTCGCTTACAGTAGCGACATCACCTAGCTTTGCGATTCA
GTGGCTGGTACCTAGACTAGCCAAGTTTAGCGAGAAAAACCCAGAGATTGACGTGCGAATTAAGGCCGTGGACGACGAGG
ATGGTTCACTCACCGACGATGTTGACGTGGCAATTTATTATGGCATGGGCAACTGGCAAGGAATGCGTGCCGATAAGCTC
AGAAACGAAGTACTTATCCCAGTTTGTTCACCTCTATTACTCAATGGTCCTAAGCCATTAGAAAAGCCCTGCGATCTTAG
AAATCATACCTTGCTACATGACTCTAGCCGCCAAGATTGGCTGGCTTGGTTTAGGCAATGTGGAATTACTGATATTAATG
TGAACCAAGGTCCGATATTTAGTCACTCCTCTCTTGTGTTGCAAGCTGCCGCACATGGTCAAGGTGTGGCGCTAGGCTAC
AGCGTACTAGCAAGACCCGATATCAAAGCGGGTCGTCTAGTTTGTCCCTTCCCTGAAGTTCTAGTCAGTAAAAGTGCATA
TTATTTAGTGTGTCAACAAAACCATGCCGAGATCGGTAAAGTGGTAGCCTTTAGAGAATGGATGCTGGATATGTTCGAAG
AGGAGACCCGTAGTGAGTTGCTTACTGGATAA

Upstream 100 bases:

>100_bases
CGTTGGGGCAAATTCTGTACAAAAAGGCTGGCCTTTACAAACGATAAAATGTAACAATAGTCATTAGAAAAACTAATTAA
AAATCGGGATCTGGATCACT

Downstream 100 bases:

>100_bases
CTTAAAGCAAGCGTATTTAGATGGCGAGTTAGCAGCGGCTGTTGCTGCGCTCAAGAGTGAGGATTTGCTCTTCGATGTTA
GTCAGTATCTTGTCGATGCT

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MARRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEYLGLKLFRRKNRSLLLTEEGQSYFLDIKDIFTQL
ADATDRLLARSAIGSLTVATSPSFAIQWLVPRLAKFSEKNPEIDVRIKAVDDEDGSLTDDVDVAIYYGMGNWQGMRADKL
RNEVLIPVCSPLLLNGPKPLEKPCDLRNHTLLHDSSRQDWLAWFRQCGITDINVNQGPIFSHSSLVLQAAAHGQGVALGY
SVLARPDIKAGRLVCPFPEVLVSKSAYYLVCQQNHAEIGKVVAFREWMLDMFEEETRSELLTG

Sequences:

>Translated_303_residues
MARRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEYLGLKLFRRKNRSLLLTEEGQSYFLDIKDIFTQL
ADATDRLLARSAIGSLTVATSPSFAIQWLVPRLAKFSEKNPEIDVRIKAVDDEDGSLTDDVDVAIYYGMGNWQGMRADKL
RNEVLIPVCSPLLLNGPKPLEKPCDLRNHTLLHDSSRQDWLAWFRQCGITDINVNQGPIFSHSSLVLQAAAHGQGVALGY
SVLARPDIKAGRLVCPFPEVLVSKSAYYLVCQQNHAEIGKVVAFREWMLDMFEEETRSELLTG
>Mature_302_residues
ARRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEYLGLKLFRRKNRSLLLTEEGQSYFLDIKDIFTQLA
DATDRLLARSAIGSLTVATSPSFAIQWLVPRLAKFSEKNPEIDVRIKAVDDEDGSLTDDVDVAIYYGMGNWQGMRADKLR
NEVLIPVCSPLLLNGPKPLEKPCDLRNHTLLHDSSRQDWLAWFRQCGITDINVNQGPIFSHSSLVLQAAAHGQGVALGYS
VLARPDIKAGRLVCPFPEVLVSKSAYYLVCQQNHAEIGKVVAFREWMLDMFEEETRSELLTG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=289, Percent_Identity=69.2041522491349, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=36.5853658536585, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1788706, Length=288, Percent_Identity=31.5972222222222, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI145693193, Length=301, Percent_Identity=26.2458471760797, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786401, Length=173, Percent_Identity=31.2138728323699, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI157672245, Length=165, Percent_Identity=32.1212121212121, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789639, Length=194, Percent_Identity=26.8041237113402, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081978, Length=267, Percent_Identity=28.8389513108614, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787879, Length=130, Percent_Identity=31.5384615384615, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787128, Length=131, Percent_Identity=32.0610687022901, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787589, Length=134, Percent_Identity=34.3283582089552, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082132, Length=156, Percent_Identity=26.2820512820513, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790208, Length=253, Percent_Identity=26.0869565217391, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI145693105, Length=122, Percent_Identity=31.9672131147541, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33896; Mature: 33765

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEYLGLKLFRRKNRSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LLTEEGQSYFLDIKDIFTQLADATDRLLARSAIGSLTVATSPSFAIQWLVPRLAKFSEKN
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCC
PEIDVRIKAVDDEDGSLTDDVDVAIYYGMGNWQGMRADKLRNEVLIPVCSPLLLNGPKPL
CCEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHHHHCCEEEEECCHHHCCCCCCC
EKPCDLRNHTLLHDSSRQDWLAWFRQCGITDINVNQGPIFSHSSLVLQAAAHGQGVALGY
CCCCCCCCCEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHEEECCCCCCEEEHH
SVLARPDIKAGRLVCPFPEVLVSKSAYYLVCQQNHAEIGKVVAFREWMLDMFEEETRSEL
HHHCCCCCCCCCEECCCHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTG
CCC
>Mature Secondary Structure 
ARRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEYLGLKLFRRKNRSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LLTEEGQSYFLDIKDIFTQLADATDRLLARSAIGSLTVATSPSFAIQWLVPRLAKFSEKN
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCC
PEIDVRIKAVDDEDGSLTDDVDVAIYYGMGNWQGMRADKLRNEVLIPVCSPLLLNGPKPL
CCEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHHHHCCEEEEECCHHHCCCCCCC
EKPCDLRNHTLLHDSSRQDWLAWFRQCGITDINVNQGPIFSHSSLVLQAAAHGQGVALGY
CCCCCCCCCEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHEEECCCCCCEEEHH
SVLARPDIKAGRLVCPFPEVLVSKSAYYLVCQQNHAEIGKVVAFREWMLDMFEEETRSEL
HHHCCCCCCCCCEECCCHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]