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The map label for this gene is thiI [H]

Identifier: 157962804

GI number: 157962804

Start: 3657742

End: 3659196

Strand: Reverse

Name: thiI [H]

Synonym: Spea_2986

Alternate gene names: 157962804

Gene position: 3659196-3657742 (Counterclockwise)

Preceding gene: 157962805

Following gene: 157962802

Centisome position: 70.71

GC content: 42.96

Gene sequence:

>1455_bases
ATGAAATTTATCGTAAAACTGTTTCCTGAAATCATGATGAAAAGCAAGCCGGTAAGAATGCGCTTTACCAAAATGCTTGA
GACTAACATTCGTAACGTACTTAAAAAAGTCGATGAGACTGCGAAAGTACAGCGCCAGTGGGACAAGATCATGGTGATGG
TACCAGATGATAGGCCTGATCTGGTTGAAGCATTTGCTGAACGTTTAGCCTGTATTCCGGGTATTGCCCATGTTTTGCAA
GTTAATGTTAGCACCTTTACAACTGTTGATGACATCTACCAACAAACCTTAGTTGCCTATAAAGAGCAATTAGCAGGCAA
AACATTTTGTGTACGTGTAAAGCGTGCAGGTAAGCATGACTTTAACTCGATTGAAGTTGAGCGCTATGTTGGTGGTGGAT
TAAATCAGTTTACCGAGGCGGCAGGTGTTCGTTTAAAAAATCCTGATATGACAGTGCACCTAGAGATCGATAATGACAAT
CTTTACCTTATCGATAAGCGTATTGAAGGCCTAGGTGGTTTCCCTATGGCGACGCAGGAAGATGTGTTGTCACTGATCTC
TGGTGGTTTTGACTCTGGTGTATCGAGTTACCAGTTTATCAAGCGTGGTTCTCGTACCCACTATTGCTTCTTTAACTTAG
GCGGCGATCAACACGAAATCGGTGTTAAGCAGGTGGCATATCATTTGTGGCAGAAATACGGTGAGTCACATAAAGTTAAG
TTTATCTCAGTACCGTTCGATCCTGTGGTTCAAGAAATTCTTGAGCGTATAGATAACGGTCAAATGGGCGTTATCTTAAA
GCGTATGATGATGCGAGCTGCAACGCGTGTTGCACAGAAGATGGGCATTCAGGCATTAGTCACCGGTGAGGCAATGGGGC
AGGTATCTAGCCAAACCTTAACTAACCTGAATGTCATCGACCGCTGTACAGAGCAGTTAATTTTACGTCCTTTGATTGCA
ATGGATAAGCAAGACATCATTAATATTAGCCGTCAAATCGGCACCGAAGACTTTGCCAAGTCAATTCCGGAATATTGTGG
CGTGATTTCTCAAAAGCCAACGGTTAAAGCGGTATTAGCGAAAGTTGAAGCCGAAGAAGCAAAGTTTTCTGAAGATCTGC
TCGACCGTGTGATTGCCGATGCTGAAATTATCGATATTAAGGAGATAGCAACGCAGATGGATACTAAAATTACCGCAACA
GAAACTGTCGATGCGATTAGTGCTGGTGAGATCATTATTGACGTCCGTGCACCAGAAGAGGAAGAGAAGTCTCCGCTAGA
GCTTGATGGTGTCGAAGTTAAAGCGATTCCTTTCTTTAAGTTAGCGACTCAGTTTGCCGACTTAGATAAAGAGAAGAATT
ACCTGCTTTATTGCGATCGTGGTGTCATGAGTAAGCTTCAGGCGCTATATCTACAGGAGCAAGGTTATAACAACGTTAAG
GTTTACCGCCCTTAA

Upstream 100 bases:

>100_bases
GGTTTTGTCGAGACAAACTGAGATTGGATATACGCAATTGCTACGCGATCTTGTTATAATTGGTGAATTGCTGTTTAAAC
TACCCCCTCGCGGAAGACCA

Downstream 100 bases:

>100_bases
TATGAGCTAACTAGCGCTTATTGGCGTAAGTATGGTAAAGGCGACTGTAAATCAGTCGCCTTTTTTGTATCTAGAGTACG
GCTGTTATTTGGTTGAGAGT

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MKFIVKLFPEIMMKSKPVRMRFTKMLETNIRNVLKKVDETAKVQRQWDKIMVMVPDDRPDLVEAFAERLACIPGIAHVLQ
VNVSTFTTVDDIYQQTLVAYKEQLAGKTFCVRVKRAGKHDFNSIEVERYVGGGLNQFTEAAGVRLKNPDMTVHLEIDNDN
LYLIDKRIEGLGGFPMATQEDVLSLISGGFDSGVSSYQFIKRGSRTHYCFFNLGGDQHEIGVKQVAYHLWQKYGESHKVK
FISVPFDPVVQEILERIDNGQMGVILKRMMMRAATRVAQKMGIQALVTGEAMGQVSSQTLTNLNVIDRCTEQLILRPLIA
MDKQDIINISRQIGTEDFAKSIPEYCGVISQKPTVKAVLAKVEAEEAKFSEDLLDRVIADAEIIDIKEIATQMDTKITAT
ETVDAISAGEIIIDVRAPEEEEKSPLELDGVEVKAIPFFKLATQFADLDKEKNYLLYCDRGVMSKLQALYLQEQGYNNVK
VYRP

Sequences:

>Translated_484_residues
MKFIVKLFPEIMMKSKPVRMRFTKMLETNIRNVLKKVDETAKVQRQWDKIMVMVPDDRPDLVEAFAERLACIPGIAHVLQ
VNVSTFTTVDDIYQQTLVAYKEQLAGKTFCVRVKRAGKHDFNSIEVERYVGGGLNQFTEAAGVRLKNPDMTVHLEIDNDN
LYLIDKRIEGLGGFPMATQEDVLSLISGGFDSGVSSYQFIKRGSRTHYCFFNLGGDQHEIGVKQVAYHLWQKYGESHKVK
FISVPFDPVVQEILERIDNGQMGVILKRMMMRAATRVAQKMGIQALVTGEAMGQVSSQTLTNLNVIDRCTEQLILRPLIA
MDKQDIINISRQIGTEDFAKSIPEYCGVISQKPTVKAVLAKVEAEEAKFSEDLLDRVIADAEIIDIKEIATQMDTKITAT
ETVDAISAGEIIIDVRAPEEEEKSPLELDGVEVKAIPFFKLATQFADLDKEKNYLLYCDRGVMSKLQALYLQEQGYNNVK
VYRP
>Mature_484_residues
MKFIVKLFPEIMMKSKPVRMRFTKMLETNIRNVLKKVDETAKVQRQWDKIMVMVPDDRPDLVEAFAERLACIPGIAHVLQ
VNVSTFTTVDDIYQQTLVAYKEQLAGKTFCVRVKRAGKHDFNSIEVERYVGGGLNQFTEAAGVRLKNPDMTVHLEIDNDN
LYLIDKRIEGLGGFPMATQEDVLSLISGGFDSGVSSYQFIKRGSRTHYCFFNLGGDQHEIGVKQVAYHLWQKYGESHKVK
FISVPFDPVVQEILERIDNGQMGVILKRMMMRAATRVAQKMGIQALVTGEAMGQVSSQTLTNLNVIDRCTEQLILRPLIA
MDKQDIINISRQIGTEDFAKSIPEYCGVISQKPTVKAVLAKVEAEEAKFSEDLLDRVIADAEIIDIKEIATQMDTKITAT
ETVDAISAGEIIIDVRAPEEEEKSPLELDGVEVKAIPFFKLATQFADLDKEKNYLLYCDRGVMSKLQALYLQEQGYNNVK
VYRP

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=484, Percent_Identity=61.7768595041322, Blast_Score=638, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 54636; Mature: 54636

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS51165 THUMP ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIVKLFPEIMMKSKPVRMRFTKMLETNIRNVLKKVDETAKVQRQWDKIMVMVPDDRPD
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHH
LVEAFAERLACIPGIAHVLQVNVSTFTTVDDIYQQTLVAYKEQLAGKTFCVRVKRAGKHD
HHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCC
FNSIEVERYVGGGLNQFTEAAGVRLKNPDMTVHLEIDNDNLYLIDKRIEGLGGFPMATQE
CCCEEEHHHHCCCHHHHHHHHCCEECCCCEEEEEEECCCCEEEEEEHHCCCCCCCCCCHH
DVLSLISGGFDSGVSSYQFIKRGSRTHYCFFNLGGDQHEIGVKQVAYHLWQKYGESHKVK
HHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEE
FISVPFDPVVQEILERIDNGQMGVILKRMMMRAATRVAQKMGIQALVTGEAMGQVSSQTL
EEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHH
TNLNVIDRCTEQLILRPLIAMDKQDIINISRQIGTEDFAKSIPEYCGVISQKPTVKAVLA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHH
KVEAEEAKFSEDLLDRVIADAEIIDIKEIATQMDTKITATETVDAISAGEIIIDVRAPEE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEHHHHHHCCCCCEEEEECCCCC
EEKSPLELDGVEVKAIPFFKLATQFADLDKEKNYLLYCDRGVMSKLQALYLQEQGYNNVK
CCCCCCEECCEEEEECHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEE
VYRP
EECC
>Mature Secondary Structure
MKFIVKLFPEIMMKSKPVRMRFTKMLETNIRNVLKKVDETAKVQRQWDKIMVMVPDDRPD
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHH
LVEAFAERLACIPGIAHVLQVNVSTFTTVDDIYQQTLVAYKEQLAGKTFCVRVKRAGKHD
HHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCC
FNSIEVERYVGGGLNQFTEAAGVRLKNPDMTVHLEIDNDNLYLIDKRIEGLGGFPMATQE
CCCEEEHHHHCCCHHHHHHHHCCEECCCCEEEEEEECCCCEEEEEEHHCCCCCCCCCCHH
DVLSLISGGFDSGVSSYQFIKRGSRTHYCFFNLGGDQHEIGVKQVAYHLWQKYGESHKVK
HHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEE
FISVPFDPVVQEILERIDNGQMGVILKRMMMRAATRVAQKMGIQALVTGEAMGQVSSQTL
EEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHH
TNLNVIDRCTEQLILRPLIAMDKQDIINISRQIGTEDFAKSIPEYCGVISQKPTVKAVLA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHH
KVEAEEAKFSEDLLDRVIADAEIIDIKEIATQMDTKITATETVDAISAGEIIIDVRAPEE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEHHHHHHCCCCCEEEEECCCCC
EEKSPLELDGVEVKAIPFFKLATQFADLDKEKNYLLYCDRGVMSKLQALYLQEQGYNNVK
CCCCCCEECCEEEEECHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEE
VYRP
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA