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The map label for this gene is phoB [H]

Identifier: 157962787

GI number: 157962787

Start: 3636287

End: 3636976

Strand: Reverse

Name: phoB [H]

Synonym: Spea_2969

Alternate gene names: 157962787

Gene position: 3636976-3636287 (Counterclockwise)

Preceding gene: 157962788

Following gene: 157962786

Centisome position: 70.29

GC content: 47.39

Gene sequence:

>690_bases
ATGACTGCAAGGATTTTGATAGTTGAAGATGAGTTAGCCATCCGAGAGATGTTAACTTTTGTTTTGGAACAGCATGGTTA
TACCACAGTCGCCGCCGAGGATTACGACTCTGCACTAGATATGTTAACCGAGCCATACCCTGATCTTGTTTTACTCGATT
GGATGTTCCCTGGTGGTAGCGGTATTCAGTTAGCTAAGCGCTTACGCCAAGACGAATTCACTCGTCATATTCCGATTATC
ATGTTGACTGCACGTGGTGAAGAGGAAGACAAGGTTCGCGGGCTAGAAGTGGGGGCCGATGATTTTATGACTAAGCCTTT
CTCTCCCAAAGAGCTAGTGGCGCGGATTAAAGCCGTTATGCGTCGCAGTGCTCCGACTTGCCTAGAAGATCCAATCGATG
TTCAAGGCCTGCAATTGGATCCTGTTAGCCACCGAGTCACAGTGGGTGATGAAGTGCTTGATATGGGACCGACTGAGTTT
CGACTGCTGCATTTCTTTATGACCCATCAAGAACGAGTTTATAGCCGTGAACAGCTACTAGACAATGTCTGGGGAACCAA
TGTGTATGTTGAAGACCGCACTGTGGATGTACATATTAGACGCTTAAGAAAAGCAGTGACTCATTCAGGGCATGATCGCC
TGATCCAAACAGTTCGCGGCGCTGGTTATCGTTTCTCTACTCGCCTCTAG

Upstream 100 bases:

>100_bases
AAATATCGCTTAATATGGCAAAATATCCCAGTGTTTAATATTAATGTCATAAAAGTGACCAATAATACCATCGTAGAAAT
TCACTGATAGAAGATCGATT

Downstream 100 bases:

>100_bases
TTGATAGGTACAAGGTGATTTTTAGCTTATCTTGTGCCGAGCTTTCTATATGGCCGATATATTGGTAAAACATGTTTGAT
TCATATTCTGGGTACCGTTT

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTARILIVEDELAIREMLTFVLEQHGYTTVAAEDYDSALDMLTEPYPDLVLLDWMFPGGSGIQLAKRLRQDEFTRHIPII
MLTARGEEEDKVRGLEVGADDFMTKPFSPKELVARIKAVMRRSAPTCLEDPIDVQGLQLDPVSHRVTVGDEVLDMGPTEF
RLLHFFMTHQERVYSREQLLDNVWGTNVYVEDRTVDVHIRRLRKAVTHSGHDRLIQTVRGAGYRFSTRL

Sequences:

>Translated_229_residues
MTARILIVEDELAIREMLTFVLEQHGYTTVAAEDYDSALDMLTEPYPDLVLLDWMFPGGSGIQLAKRLRQDEFTRHIPII
MLTARGEEEDKVRGLEVGADDFMTKPFSPKELVARIKAVMRRSAPTCLEDPIDVQGLQLDPVSHRVTVGDEVLDMGPTEF
RLLHFFMTHQERVYSREQLLDNVWGTNVYVEDRTVDVHIRRLRKAVTHSGHDRLIQTVRGAGYRFSTRL
>Mature_228_residues
TARILIVEDELAIREMLTFVLEQHGYTTVAAEDYDSALDMLTEPYPDLVLLDWMFPGGSGIQLAKRLRQDEFTRHIPIIM
LTARGEEEDKVRGLEVGADDFMTKPFSPKELVARIKAVMRRSAPTCLEDPIDVQGLQLDPVSHRVTVGDEVLDMGPTEFR
LLHFFMTHQERVYSREQLLDNVWGTNVYVEDRTVDVHIRRLRKAVTHSGHDRLIQTVRGAGYRFSTRL

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=72.9257641921397, Blast_Score=356, Evalue=1e-100,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=38.9380530973451, Blast_Score=151, Evalue=3e-38,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=36.8181818181818, Blast_Score=144, Evalue=3e-36,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=36.2445414847162, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=36.036036036036, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=35.8744394618834, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=36.1990950226244, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1790863, Length=238, Percent_Identity=36.5546218487395, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI2367329, Length=234, Percent_Identity=39.7435897435897, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=32.8888888888889, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=35.4545454545455, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1789402, Length=226, Percent_Identity=31.4159292035398, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=32.6086956521739, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI145693140, Length=239, Percent_Identity=29.7071129707113, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788550, Length=114, Percent_Identity=41.2280701754386, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=27.5, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1790299, Length=115, Percent_Identity=32.1739130434783, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26310; Mature: 26178

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARILIVEDELAIREMLTFVLEQHGYTTVAAEDYDSALDMLTEPYPDLVLLDWMFPGGS
CCEEEEEEECHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCEEEEEEECCCCC
GIQLAKRLRQDEFTRHIPIIMLTARGEEEDKVRGLEVGADDFMTKPFSPKELVARIKAVM
HHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCEEECCHHHHCCCCCHHHHHHHHHHHH
RRSAPTCLEDPIDVQGLQLDPVSHRVTVGDEVLDMGPTEFRLLHFFMTHQERVYSREQLL
HHCCCCCCCCCCCCCCEEECCCCCEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
DNVWGTNVYVEDRTVDVHIRRLRKAVTHSGHDRLIQTVRGAGYRFSTRL
HHHCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCC
>Mature Secondary Structure 
TARILIVEDELAIREMLTFVLEQHGYTTVAAEDYDSALDMLTEPYPDLVLLDWMFPGGS
CEEEEEEECHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCEEEEEEECCCCC
GIQLAKRLRQDEFTRHIPIIMLTARGEEEDKVRGLEVGADDFMTKPFSPKELVARIKAVM
HHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCEEECCHHHHCCCCCHHHHHHHHHHHH
RRSAPTCLEDPIDVQGLQLDPVSHRVTVGDEVLDMGPTEFRLLHFFMTHQERVYSREQLL
HHCCCCCCCCCCCCCCEEECCCCCEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
DNVWGTNVYVEDRTVDVHIRRLRKAVTHSGHDRLIQTVRGAGYRFSTRL
HHHCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]