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The map label for this gene is pdhA [H]

Identifier: 157962067

GI number: 157962067

Start: 2723827

End: 2725005

Strand: Reverse

Name: pdhA [H]

Synonym: Spea_2246

Alternate gene names: 157962067

Gene position: 2725005-2723827 (Counterclockwise)

Preceding gene: 157962068

Following gene: 157962066

Centisome position: 52.66

GC content: 45.12

Gene sequence:

>1179_bases
ATGAGCAACGCAACAACGAATGCTGAAACAGTCCATCGCGTGAGCTTTTTAGATAAAGACTCACTCGCGATCCCCATTTT
AAAAATTCTCCAAGCGGACGGTACCGCTTATGAAGAGGCCGTATTACCCGTTATCGATGAAGCTTTAGCGGCTAAAATCC
ATGATACCTGCGTATTTACCCGTGTATTAGACGAGCGTATGTTGGGGGCGCAGCGTCAAGGTCGTATTAGCTTTTATATG
ACCTGTACTGGTGAAGAGGCGTCGATAATAGGCAGCACTGCAGCACTCGATGATGGTGATGTGATCTTGGCTCAGTACCG
TGAACATGCTGCGATTCGTTACCGCGGATTTACCACTGAGCAGTTTATGAATCAGATGTTCAGTAATGAAAAAGATTTTG
GCAAGGGCCGCCAGATGCCGATCCATTACGGCAGTGAAGCGCTTAACTATCAAACTATTTCATCTCCGCTTGCCACACAG
ATCCCTCAAGCTACAGGTGTTGCATATAGCCTAAAAATGCAGGGTAAGCGTAATGTAGCAATCTGTTATTTTGGTGAAGG
CGCAGCCTCAGAAGGCGATTTTCACGCCGGTCTAAATATGGCAGCAGTGCTCAATTCTCCAGTTATTTTCTTTTGCCGTA
ATAACGGTTACGCCATCTCAACACCTACTGATGAACAGTTTAAAGGTAACGGTATTGCTAGCCGCGGTGTGGGCTATGGC
ATGCATACCATTCGTGTCGATGGTAACGACATGTTGGCAGTATTAGCGGCAACTCAACAGGCTCGCGCCTATGCGTTAGA
CAATAATGCCCCTGTTTTGATTGAAGCCATGACTTATCGATTAGGTGCTCATTCATCATCTGATGATCCTTCTGGCTATC
GCTCGAAAGATGAAGAGGCAAAATGGCAGCAGCATGATCCAGTTAAACGTTTCAAGCTATGGATGATCAATAAGGGCTGG
ATGAATGAGCAGCGCGATGCCGATCTCTATGTTAAGTACCGTGAAGAGGTGCTATCTGAGCTTAAGGTTGCTGAAAAAAT
TCCAACTCCTCATATCGATAACATTATCGAAGATGTATATGACACACCAACCCCAATTCTGAAGAAGCAGCTAGCTGACT
TAAAAAACCATGTTAAGAAGTATCCAGAATCGTATCCAAAAAGTGCAGGGAGAGTATAA

Upstream 100 bases:

>100_bases
TGAGAGTTAGTTTACGTTAACGTAATGTTCTTCCCGCGTATTAAAATAGAATGGTGCTGTATTTATCGTTTAACGGCACC
CTCAATAACAAGAGCACATT

Downstream 100 bases:

>100_bases
GCCGTGGCTCAGATGAATATGTTGCAAGCCATTAACCAAGCCCTAAGCTCGGAAATGGAAGCGGATAAAAAAATGATGGT
GTTCGGCGAAGACGTCGGCC

Product: 3-methyl-2-oxobutanoate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSNATTNAETVHRVSFLDKDSLAIPILKILQADGTAYEEAVLPVIDEALAAKIHDTCVFTRVLDERMLGAQRQGRISFYM
TCTGEEASIIGSTAALDDGDVILAQYREHAAIRYRGFTTEQFMNQMFSNEKDFGKGRQMPIHYGSEALNYQTISSPLATQ
IPQATGVAYSLKMQGKRNVAICYFGEGAASEGDFHAGLNMAAVLNSPVIFFCRNNGYAISTPTDEQFKGNGIASRGVGYG
MHTIRVDGNDMLAVLAATQQARAYALDNNAPVLIEAMTYRLGAHSSSDDPSGYRSKDEEAKWQQHDPVKRFKLWMINKGW
MNEQRDADLYVKYREEVLSELKVAEKIPTPHIDNIIEDVYDTPTPILKKQLADLKNHVKKYPESYPKSAGRV

Sequences:

>Translated_392_residues
MSNATTNAETVHRVSFLDKDSLAIPILKILQADGTAYEEAVLPVIDEALAAKIHDTCVFTRVLDERMLGAQRQGRISFYM
TCTGEEASIIGSTAALDDGDVILAQYREHAAIRYRGFTTEQFMNQMFSNEKDFGKGRQMPIHYGSEALNYQTISSPLATQ
IPQATGVAYSLKMQGKRNVAICYFGEGAASEGDFHAGLNMAAVLNSPVIFFCRNNGYAISTPTDEQFKGNGIASRGVGYG
MHTIRVDGNDMLAVLAATQQARAYALDNNAPVLIEAMTYRLGAHSSSDDPSGYRSKDEEAKWQQHDPVKRFKLWMINKGW
MNEQRDADLYVKYREEVLSELKVAEKIPTPHIDNIIEDVYDTPTPILKKQLADLKNHVKKYPESYPKSAGRV
>Mature_391_residues
SNATTNAETVHRVSFLDKDSLAIPILKILQADGTAYEEAVLPVIDEALAAKIHDTCVFTRVLDERMLGAQRQGRISFYMT
CTGEEASIIGSTAALDDGDVILAQYREHAAIRYRGFTTEQFMNQMFSNEKDFGKGRQMPIHYGSEALNYQTISSPLATQI
PQATGVAYSLKMQGKRNVAICYFGEGAASEGDFHAGLNMAAVLNSPVIFFCRNNGYAISTPTDEQFKGNGIASRGVGYGM
HTIRVDGNDMLAVLAATQQARAYALDNNAPVLIEAMTYRLGAHSSSDDPSGYRSKDEEAKWQQHDPVKRFKLWMINKGWM
NEQRDADLYVKYREEVLSELKVAEKIPTPHIDNIIEDVYDTPTPILKKQLADLKNHVKKYPESYPKSAGRV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11386135, Length=382, Percent_Identity=46.8586387434555, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI258645172, Length=382, Percent_Identity=46.8586387434555, Blast_Score=358, Evalue=4e-99,
Organism=Homo sapiens, GI291084742, Length=290, Percent_Identity=28.6206896551724, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4505685, Length=290, Percent_Identity=28.6206896551724, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI4885543, Length=358, Percent_Identity=25.1396648044693, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI291084744, Length=283, Percent_Identity=28.2685512367491, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI291084757, Length=291, Percent_Identity=25.4295532646048, Blast_Score=97, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI86563355, Length=369, Percent_Identity=50.9485094850949, Blast_Score=388, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI86563357, Length=369, Percent_Identity=50.9485094850949, Blast_Score=388, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17536047, Length=285, Percent_Identity=29.8245614035088, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32564172, Length=285, Percent_Identity=29.8245614035088, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321026, Length=307, Percent_Identity=27.3615635179153, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21355903, Length=371, Percent_Identity=46.0916442048518, Blast_Score=349, Evalue=2e-96,
Organism=Drosophila melanogaster, GI24639746, Length=300, Percent_Identity=28.3333333333333, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24639740, Length=300, Percent_Identity=28.3333333333333, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24639744, Length=300, Percent_Identity=28.3333333333333, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28571106, Length=300, Percent_Identity=28.3333333333333, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24639748, Length=278, Percent_Identity=28.4172661870504, Blast_Score=115, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR017596 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 43569; Mature: 43438

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNATTNAETVHRVSFLDKDSLAIPILKILQADGTAYEEAVLPVIDEALAAKIHDTCVFT
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RVLDERMLGAQRQGRISFYMTCTGEEASIIGSTAALDDGDVILAQYREHAAIRYRGFTTE
HHHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCEEEEEHHCCCEEEECCCCHH
QFMNQMFSNEKDFGKGRQMPIHYGSEALNYQTISSPLATQIPQATGVAYSLKMQGKRNVA
HHHHHHHCCCCCCCCCCCCCEEECCHHCCHHHHCCCHHHHCCCCCCCEEEEEECCCCCEE
ICYFGEGAASEGDFHAGLNMAAVLNSPVIFFCRNNGYAISTPTDEQFKGNGIASRGVGYG
EEEECCCCCCCCCCCCCCCEEEECCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCC
MHTIRVDGNDMLAVLAATQQARAYALDNNAPVLIEAMTYRLGAHSSSDDPSGYRSKDEEA
EEEEEECCCCEEEEEEHHHHHHEEEECCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCCC
KWQQHDPVKRFKLWMINKGWMNEQRDADLYVKYREEVLSELKVAEKIPTPHIDNIIEDVY
CCCCCCCHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
DTPTPILKKQLADLKNHVKKYPESYPKSAGRV
CCCCHHHHHHHHHHHHHHHHCHHHCCCCCCCC
>Mature Secondary Structure 
SNATTNAETVHRVSFLDKDSLAIPILKILQADGTAYEEAVLPVIDEALAAKIHDTCVFT
CCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RVLDERMLGAQRQGRISFYMTCTGEEASIIGSTAALDDGDVILAQYREHAAIRYRGFTTE
HHHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCEEEEEHHCCCEEEECCCCHH
QFMNQMFSNEKDFGKGRQMPIHYGSEALNYQTISSPLATQIPQATGVAYSLKMQGKRNVA
HHHHHHHCCCCCCCCCCCCCEEECCHHCCHHHHCCCHHHHCCCCCCCEEEEEECCCCCEE
ICYFGEGAASEGDFHAGLNMAAVLNSPVIFFCRNNGYAISTPTDEQFKGNGIASRGVGYG
EEEECCCCCCCCCCCCCCCEEEECCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCC
MHTIRVDGNDMLAVLAATQQARAYALDNNAPVLIEAMTYRLGAHSSSDDPSGYRSKDEEA
EEEEEECCCCEEEEEEHHHHHHEEEECCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCCC
KWQQHDPVKRFKLWMINKGWMNEQRDADLYVKYREEVLSELKVAEKIPTPHIDNIIEDVY
CCCCCCCHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
DTPTPILKKQLADLKNHVKKYPESYPKSAGRV
CCCCHHHHHHHHHHHHHHHHCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA