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The map label for this gene is 157962066

Identifier: 157962066

GI number: 157962066

Start: 2722846

End: 2723823

Strand: Reverse

Name: 157962066

Synonym: Spea_2245

Alternate gene names: NA

Gene position: 2723823-2722846 (Counterclockwise)

Preceding gene: 157962067

Following gene: 157962065

Centisome position: 52.64

GC content: 48.26

Gene sequence:

>978_bases
GTGGCTCAGATGAATATGTTGCAAGCCATTAACCAAGCCCTAAGCTCGGAAATGGAAGCGGATAAAAAAATGATGGTGTT
CGGCGAAGACGTCGGCCACTTTGGTGGGGTATTTAGAGCAACTTCGGGCCTACAGGAAAAGTTTGGCCGTGAGCGTTGCT
TCAATACACCGTTAACCGAGCAGGGCATTGCCGGTTTCGCCAATGGCTTAGCCTCGAACGGCATGACGGCGGTGGCTGAA
ATTCAGTTTGCCGATTATATCTTTCCTGCCATCGATCAGATAGTGAATGAGAGTGCCAAGTTCCGTTACCGCAGCGGTAA
CGAGTTTAATGTTGGCGGCCTTACTTTTAGAACACCTTACGGCGGCGGTATTGCAGGTGGTCATTATCACTCGCAATCAC
CAGAAGCCTATTTTACCCAAACACCGGGTCTCAAAGTTGTCGTTCCACGTAACCCTGAGCAGGCAAAAGGGCTGTTAATC
GCCTCTATTCGTGATAAAAACCCGGTGATATTTTTTGAGCCAAAGCGCTTATACCGTGCATCGGTGGGTGAAGTGCCTGA
GGGCGACCATGTTATCGAACTCGGTAAAGCCGAAGTTATCAAACAAGGTACAGACATCACCTTACTTGGCTGGGGCGCAC
AGATGGAAATCTTAGAAAACGCCGCAGAGATGGCAGCGAAGAAAGGGATCTCCTGTGAAATTATCGATTTAAGAACCTTG
TCCCCATGGGATGTTGACACGGTAGCAGCATCGGTTAAAAAGACTGGGCGTCTATTGATCAATCATGAGGCGCCGTTAAC
GGGTGGCTTTGCCGGTGAGATCGCCGCGACAATTCAAGAAGAGTGTTTCTTATATCTTGAGTCGCCGATAGCGCGAGTGT
GTGGTCTAGATACGCCGTATCCTTTGATCCACGAAAAAGAATATATGCCAGATGCGTTAAAGACGTTTGAAGCGATTAAA
GCTTCGGTAAAATTTTAG

Upstream 100 bases:

>100_bases
CACACCAACCCCAATTCTGAAGAAGCAGCTAGCTGACTTAAAAAACCATGTTAAGAAGTATCCAGAATCGTATCCAAAAA
GTGCAGGGAGAGTATAAGCC

Downstream 100 bases:

>100_bases
GAGCCAGCCATGATTAAAGAATTTATCCTTCCGGATATCGGCGAAGGCGTTGTCGAATGTGAGCTGGTTGAATGGTTAGT
ACAAGAGGGTGACAGTGTTG

Product: transketolase central region

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MAQMNMLQAINQALSSEMEADKKMMVFGEDVGHFGGVFRATSGLQEKFGRERCFNTPLTEQGIAGFANGLASNGMTAVAE
IQFADYIFPAIDQIVNESAKFRYRSGNEFNVGGLTFRTPYGGGIAGGHYHSQSPEAYFTQTPGLKVVVPRNPEQAKGLLI
ASIRDKNPVIFFEPKRLYRASVGEVPEGDHVIELGKAEVIKQGTDITLLGWGAQMEILENAAEMAAKKGISCEIIDLRTL
SPWDVDTVAASVKKTGRLLINHEAPLTGGFAGEIAATIQEECFLYLESPIARVCGLDTPYPLIHEKEYMPDALKTFEAIK
ASVKF

Sequences:

>Translated_325_residues
MAQMNMLQAINQALSSEMEADKKMMVFGEDVGHFGGVFRATSGLQEKFGRERCFNTPLTEQGIAGFANGLASNGMTAVAE
IQFADYIFPAIDQIVNESAKFRYRSGNEFNVGGLTFRTPYGGGIAGGHYHSQSPEAYFTQTPGLKVVVPRNPEQAKGLLI
ASIRDKNPVIFFEPKRLYRASVGEVPEGDHVIELGKAEVIKQGTDITLLGWGAQMEILENAAEMAAKKGISCEIIDLRTL
SPWDVDTVAASVKKTGRLLINHEAPLTGGFAGEIAATIQEECFLYLESPIARVCGLDTPYPLIHEKEYMPDALKTFEAIK
ASVKF
>Mature_324_residues
AQMNMLQAINQALSSEMEADKKMMVFGEDVGHFGGVFRATSGLQEKFGRERCFNTPLTEQGIAGFANGLASNGMTAVAEI
QFADYIFPAIDQIVNESAKFRYRSGNEFNVGGLTFRTPYGGGIAGGHYHSQSPEAYFTQTPGLKVVVPRNPEQAKGLLIA
SIRDKNPVIFFEPKRLYRASVGEVPEGDHVIELGKAEVIKQGTDITLLGWGAQMEILENAAEMAAKKGISCEIIDLRTLS
PWDVDTVAASVKKTGRLLINHEAPLTGGFAGEIAATIQEECFLYLESPIARVCGLDTPYPLIHEKEYMPDALKTFEAIKA
SVKF

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=324, Percent_Identity=59.5679012345679, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI34101272, Length=324, Percent_Identity=59.5679012345679, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI156564403, Length=329, Percent_Identity=36.4741641337386, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI291084858, Length=329, Percent_Identity=34.9544072948328, Blast_Score=195, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI17506935, Length=324, Percent_Identity=58.0246913580247, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17538422, Length=330, Percent_Identity=36.6666666666667, Blast_Score=207, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6319698, Length=324, Percent_Identity=36.4197530864198, Blast_Score=205, Evalue=7e-54,
Organism=Drosophila melanogaster, GI160714828, Length=324, Percent_Identity=57.4074074074074, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI160714832, Length=324, Percent_Identity=57.4074074074074, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI21358145, Length=303, Percent_Identity=37.6237623762376, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24650940, Length=303, Percent_Identity=37.6237623762376, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI160714826, Length=65, Percent_Identity=53.8461538461538, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=36.2637362637363, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=36.2637362637363, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 35448; Mature: 35317

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQMNMLQAINQALSSEMEADKKMMVFGEDVGHFGGVFRATSGLQEKFGRERCFNTPLTE
CCHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
QGIAGFANGLASNGMTAVAEIQFADYIFPAIDQIVNESAKFRYRSGNEFNVGGLTFRTPY
HHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEECCCCEEECCCEEEECCC
GGGIAGGHYHSQSPEAYFTQTPGLKVVVPRNPEQAKGLLIASIRDKNPVIFFEPKRLYRA
CCCCCCCCCCCCCCCEEEECCCCEEEEECCCCHHHCCEEEEEECCCCCEEEECCHHHHHH
SVGEVPEGDHVIELGKAEVIKQGTDITLLGWGAQMEILENAAEMAAKKGISCEIIDLRTL
HCCCCCCCCCEEECCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEECC
SPWDVDTVAASVKKTGRLLINHEAPLTGGFAGEIAATIQEECFLYLESPIARVCGLDTPY
CCCCHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PLIHEKEYMPDALKTFEAIKASVKF
CCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AQMNMLQAINQALSSEMEADKKMMVFGEDVGHFGGVFRATSGLQEKFGRERCFNTPLTE
CHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
QGIAGFANGLASNGMTAVAEIQFADYIFPAIDQIVNESAKFRYRSGNEFNVGGLTFRTPY
HHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEECCCCEEECCCEEEECCC
GGGIAGGHYHSQSPEAYFTQTPGLKVVVPRNPEQAKGLLIASIRDKNPVIFFEPKRLYRA
CCCCCCCCCCCCCCCEEEECCCCEEEEECCCCHHHCCEEEEEECCCCCEEEECCHHHHHH
SVGEVPEGDHVIELGKAEVIKQGTDITLLGWGAQMEILENAAEMAAKKGISCEIIDLRTL
HCCCCCCCCCEEECCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEECC
SPWDVDTVAASVKKTGRLLINHEAPLTGGFAGEIAATIQEECFLYLESPIARVCGLDTPY
CCCCHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PLIHEKEYMPDALKTFEAIKASVKF
CCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA