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The map label for this gene is astE

Identifier: 157962068

GI number: 157962068

Start: 2725188

End: 2726225

Strand: Reverse

Name: astE

Synonym: Spea_2247

Alternate gene names: 157962068

Gene position: 2726225-2725188 (Counterclockwise)

Preceding gene: 157962070

Following gene: 157962067

Centisome position: 52.68

GC content: 41.04

Gene sequence:

>1038_bases
GTGTTTCAACAATTGAAACAGTCTAAGGACTTTTTAGCGTTAACCCTCGCGCATCCACAATACTTAACAGAAAGTTTTGA
ATTTGATCTTGCTAACCAAGTCCATGTAGAGGTTTGGGATACAGGCGTTATCATGTTCGAGCCTTTAGGCGTTAAACACA
CTAAAGATCTCGTCTTATCTTGTGCCGTTCACGGTAATGAGACTGCGCCTATTGAATTGTGTAACGACCTAATGACACAA
TTACTTGATGAACAACTTGTTCTCAAGCAAAGAGTATTATTTCTTATCGGTAATCCTCCCGCCATTCATAATGGCACACG
TTTTATCGATGAAAACCTTAATCGGTTATTTAATGGTGCTCATTCACGTGGAGAGGGGCTTTGTAACCCTGAGCGAGTGA
GAGCACAAAAGCTTGAGCAATATGTCGACAAGTTTTTCAGCGCCCATTCGGGAGAGCGTCATCGAATGCATTACGATTTA
CATACTGCAATTCGGGCCTCTAAGCATGAGAAGTTTGCTATTTACCCCTATCGTGGTAATAGAAAGTACAGCAAAGAACA
GATTATGTTTCTCGAATCCTGCGGCGTGAATACCATTTTATTCCACCATGAGCCAACCACGACCTTTAGTTATTTCTCTT
CTGAAAATTATCATGCAGATGCCTTTACTATCGAACTAGGTAAAGTGTTCCCTATGGGACAAAATGATATGACTCGTTTT
ATCGCGATGAAAGAGATGCTAACTCTATTGATGTGTGGCGAAGAGCTTAAATTACCAAGTTTTGATATGAAGCGTTTAAA
CCTTTATCAAGTTTGTCGTTCGGTAAACAAACGATACGACGACTTTGAATTTACTTTCACCAACGATGTCGAAAACTTTA
CCGCTTTCCCTAGAGGCTACACTTTAGCGAAAGAGGGCGGTTTTGAGGTGAAAGTTGAGCATGAGTTTGAATCCATTGTA
TTCCCCAATGCTAAGGTGCCAGTAGGGCAGCGGACAGTCTTATGTTTAAAGTCCGCAGATGAAAGTCGTTTAGACTAG

Upstream 100 bases:

>100_bases
TACTATCGCTGACAGAATTAGCGAAGCTAGGTGTTACAAATACCCGTGGTATTTGAAATTTCTGCCAAATAAAGCTGACA
TGAATAATAAGGAGTAGTTT

Downstream 100 bases:

>100_bases
ATTCGAACTGAATAACTGTGCATGTAAACCAATATATACATGCACAGAGATGCTTAGTTGTCGTTTCAATGATGGGAAGT
ATTGAGAGTTAGTTTACGTT

Product: succinylglutamate desuccinylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MFQQLKQSKDFLALTLAHPQYLTESFEFDLANQVHVEVWDTGVIMFEPLGVKHTKDLVLSCAVHGNETAPIELCNDLMTQ
LLDEQLVLKQRVLFLIGNPPAIHNGTRFIDENLNRLFNGAHSRGEGLCNPERVRAQKLEQYVDKFFSAHSGERHRMHYDL
HTAIRASKHEKFAIYPYRGNRKYSKEQIMFLESCGVNTILFHHEPTTTFSYFSSENYHADAFTIELGKVFPMGQNDMTRF
IAMKEMLTLLMCGEELKLPSFDMKRLNLYQVCRSVNKRYDDFEFTFTNDVENFTAFPRGYTLAKEGGFEVKVEHEFESIV
FPNAKVPVGQRTVLCLKSADESRLD

Sequences:

>Translated_345_residues
MFQQLKQSKDFLALTLAHPQYLTESFEFDLANQVHVEVWDTGVIMFEPLGVKHTKDLVLSCAVHGNETAPIELCNDLMTQ
LLDEQLVLKQRVLFLIGNPPAIHNGTRFIDENLNRLFNGAHSRGEGLCNPERVRAQKLEQYVDKFFSAHSGERHRMHYDL
HTAIRASKHEKFAIYPYRGNRKYSKEQIMFLESCGVNTILFHHEPTTTFSYFSSENYHADAFTIELGKVFPMGQNDMTRF
IAMKEMLTLLMCGEELKLPSFDMKRLNLYQVCRSVNKRYDDFEFTFTNDVENFTAFPRGYTLAKEGGFEVKVEHEFESIV
FPNAKVPVGQRTVLCLKSADESRLD
>Mature_345_residues
MFQQLKQSKDFLALTLAHPQYLTESFEFDLANQVHVEVWDTGVIMFEPLGVKHTKDLVLSCAVHGNETAPIELCNDLMTQ
LLDEQLVLKQRVLFLIGNPPAIHNGTRFIDENLNRLFNGAHSRGEGLCNPERVRAQKLEQYVDKFFSAHSGERHRMHYDL
HTAIRASKHEKFAIYPYRGNRKYSKEQIMFLESCGVNTILFHHEPTTTFSYFSSENYHADAFTIELGKVFPMGQNDMTRF
IAMKEMLTLLMCGEELKLPSFDMKRLNLYQVCRSVNKRYDDFEFTFTNDVENFTAFPRGYTLAKEGGFEVKVEHEFESIV
FPNAKVPVGQRTVLCLKSADESRLD

Specific function: Transforms N(2)-succinylglutamate into succinate and glutamate

COG id: COG2988

COG function: function code E; Succinylglutamate desuccinylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily

Homologues:

Organism=Escherichia coli, GI1788040, Length=329, Percent_Identity=34.0425531914894, Blast_Score=193, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASTE_SHEPA (A8H4T0)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001502102.1
- ProteinModelPortal:   A8H4T0
- SMR:   A8H4T0
- STRING:   A8H4T0
- GeneID:   5662640
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_2247
- NMPDR:   fig|398579.3.peg.1926
- eggNOG:   COG2988
- HOGENOM:   HBG298271
- OMA:   RWRWLGH
- ProtClustDB:   PRK05324
- BioCyc:   SPEA398579:SPEA_2247-MONOMER
- HAMAP:   MF_00767
- InterPro:   IPR007036
- InterPro:   IPR016681
- PIRSF:   PIRSF017020
- TIGRFAMs:   TIGR03242

Pfam domain/function: PF04952 AstE_AspA

EC number: =3.5.1.96

Molecular weight: Translated: 39874; Mature: 39874

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: ACT_SITE 225-225

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQQLKQSKDFLALTLAHPQYLTESFEFDLANQVHVEVWDTGVIMFEPLGVKHTKDLVLS
CCHHHHCCCCEEEEEECCHHHHHHHHCCCCCCEEEEEEEECCEEEEECCCCCCCHHEEEE
CAVHGNETAPIELCNDLMTQLLDEQLVLKQRVLFLIGNPPAIHNGTRFIDENLNRLFNGA
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHCCC
HSRGEGLCNPERVRAQKLEQYVDKFFSAHSGERHRMHYDLHTAIRASKHEKFAIYPYRGN
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCCEEEEEEECCC
RKYSKEQIMFLESCGVNTILFHHEPTTTFSYFSSENYHADAFTIELGKVFPMGQNDMTRF
CCCCHHHEEEEECCCCEEEEEECCCCCEEEEECCCCCCCEEEEEEECCCCCCCCHHHHHH
IAMKEMLTLLMCGEELKLPSFDMKRLNLYQVCRSVNKRYDDFEFTFTNDVENFTAFPRGY
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHCCCCCCE
TLAKEGGFEVKVEHEFESIVFPNAKVPVGQRTVLCLKSADESRLD
EEECCCCEEEEEECCCCEEECCCCCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MFQQLKQSKDFLALTLAHPQYLTESFEFDLANQVHVEVWDTGVIMFEPLGVKHTKDLVLS
CCHHHHCCCCEEEEEECCHHHHHHHHCCCCCCEEEEEEEECCEEEEECCCCCCCHHEEEE
CAVHGNETAPIELCNDLMTQLLDEQLVLKQRVLFLIGNPPAIHNGTRFIDENLNRLFNGA
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHCCC
HSRGEGLCNPERVRAQKLEQYVDKFFSAHSGERHRMHYDLHTAIRASKHEKFAIYPYRGN
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCCEEEEEEECCC
RKYSKEQIMFLESCGVNTILFHHEPTTTFSYFSSENYHADAFTIELGKVFPMGQNDMTRF
CCCCHHHEEEEECCCCEEEEEECCCCCEEEEECCCCCCCEEEEEEECCCCCCCCHHHHHH
IAMKEMLTLLMCGEELKLPSFDMKRLNLYQVCRSVNKRYDDFEFTFTNDVENFTAFPRGY
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHCCCCCCE
TLAKEGGFEVKVEHEFESIVFPNAKVPVGQRTVLCLKSADESRLD
EEECCCCEEEEEECCCCEEECCCCCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA