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The map label for this gene is pdhC [H]

Identifier: 157962065

GI number: 157962065

Start: 2721214

End: 2722836

Strand: Reverse

Name: pdhC [H]

Synonym: Spea_2244

Alternate gene names: 157962065

Gene position: 2722836-2721214 (Counterclockwise)

Preceding gene: 157962066

Following gene: 157962064

Centisome position: 52.62

GC content: 48.74

Gene sequence:

>1623_bases
ATGATTAAAGAATTTATCCTTCCGGATATCGGCGAAGGCGTTGTCGAATGTGAGCTGGTTGAATGGTTAGTACAAGAGGG
TGACAGTGTTGTTGAAGATCAACCTATCGCCGATGTCATGACCGATAAGGCCTTAGTACAAATTCCAGCACCAAATGCAG
GAGTGATCACTAAGCTCTATTACGCTAAGGGCGAGATTGCTAAGGTGCACGCGCCGCTTTACTCGGTGGATGTTGAAGGC
GAAGCTGACACTGGCGGTCAAGCTACGGCTTCTCAAAATACAGCGACTGAAGACGCCGCTATCTCTGAGGTCGAATCAGA
GTCTGGTTCAGATAATCAAATCTCGCAGAGCGTAACGCCTGGCACGACTATCGAAGAGTTTTTACTCCCCGATATTGGCG
AAGGGATCGTTGAGTGTGAGTTGGTCGAGTGGCTAGTGAGCGAAGGTGATACCGTTGTTGAAGATCAGCCGATAGCCGAT
GTCATGACTGATAAGGCTTTAGTGCAGATACCGGCGATTAAAAACGGCAAAATTGTAAAGCTGCGTGTTCGTAAGGGGCA
GCTTGCAAAAGTGCACGCTCCGCTGTTTGCCATTGAAGTCGAGGCCGAGGTTGGAGCTGGAGAGCTGCAAGCAGCGTCTC
CTCTAATTCCAGAGTCTGGTTCATCGAGTCAATCTCTCGAGGCAACGCCACAAGGAAAAGCCTTAGCAAGTCCAGCAGTT
CGCCGCTTAGCGCGCTCATTGGATCTCGATATTTCAACAATTTCGGGTTCAGGTAAAAATGGCCGCGTCTATAAAGAAGA
TGTTGAACGTCATCATGCCAATGGTGGCCAGTCAAATATCTCGGTAACGCAAAAGTCTGTTGCAGCTCCTGCAGTTTCAA
ATCCTGCAGCTGTTATTGAGCAACCAAGCCATCGCCAAGCCGATAGGGTGGAGCCGATAAAAGGCGTTCGTGCGGTCATG
GCGAAGATGATGAGCGAATCGGTTTCGACGATTCCGCATTTCACCTACTGTGAAGAATTTGATTTAACTGAATTAGTCGC
ACTTCGTGAAAGCGTGAAGAAAAAGTATTCGACCGATGAGCTTAAACTCACCATGATGCCTTTCTTCATGAAGTCTATGT
CACTGGCGCTATCACAGTTCCCAGATATGAATAGCCGTGTGAACGGCGATTGCACCGAGCAGACCTATTTGGCGAGCCAC
AATATCGGCATGGCTGTTGACTCTAAGGTCGGCTTACTCGTGCCTAACGTCAAAGATGTGCAAGATAAAACAATCTTGCA
AATCGCTGCTGAGATCACTCGTCTAACCACCGCTGCGAGAAGCGGCCGCGTATCGCCGAATGATTTGAAAGGAGGAAGTA
TCTCCATTTCAAATATTGGCGCCTTGGGTGGTACGGTGGCCACGCCTATCATCAATAAACCTGAAGTTGCGATTGTTGCC
TTAGGTAAATTGCAAGTTCTGCCACGTTTTAACGACAAAGGCGAAGTTGAAGCACGCAAGATTATGCAGGTGAGCTGGTC
TGGGGATCATCGTGTTATCGACGGCGGCACCATTGCACGCTTCTGTAATCTGTGGAAGCTGTACCTTGAGCAGCCGCAAG
AGATGTTGTTAGCGATGCAGTAA

Upstream 100 bases:

>100_bases
AGATACGCCGTATCCTTTGATCCACGAAAAAGAATATATGCCAGATGCGTTAAAGACGTTTGAAGCGATTAAAGCTTCGG
TAAAATTTTAGGAGCCAGCC

Downstream 100 bases:

>100_bases
TCGATATAGGACTCCTTCAAAGGCGCTAGAAATAGCGCCTTTTTTATTGGCAGCGTCGCTATAAGAGTTTTAAATTTGTG
AAGTATTCCGTATAATTCCG

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MIKEFILPDIGEGVVECELVEWLVQEGDSVVEDQPIADVMTDKALVQIPAPNAGVITKLYYAKGEIAKVHAPLYSVDVEG
EADTGGQATASQNTATEDAAISEVESESGSDNQISQSVTPGTTIEEFLLPDIGEGIVECELVEWLVSEGDTVVEDQPIAD
VMTDKALVQIPAIKNGKIVKLRVRKGQLAKVHAPLFAIEVEAEVGAGELQAASPLIPESGSSSQSLEATPQGKALASPAV
RRLARSLDLDISTISGSGKNGRVYKEDVERHHANGGQSNISVTQKSVAAPAVSNPAAVIEQPSHRQADRVEPIKGVRAVM
AKMMSESVSTIPHFTYCEEFDLTELVALRESVKKKYSTDELKLTMMPFFMKSMSLALSQFPDMNSRVNGDCTEQTYLASH
NIGMAVDSKVGLLVPNVKDVQDKTILQIAAEITRLTTAARSGRVSPNDLKGGSISISNIGALGGTVATPIINKPEVAIVA
LGKLQVLPRFNDKGEVEARKIMQVSWSGDHRVIDGGTIARFCNLWKLYLEQPQEMLLAMQ

Sequences:

>Translated_540_residues
MIKEFILPDIGEGVVECELVEWLVQEGDSVVEDQPIADVMTDKALVQIPAPNAGVITKLYYAKGEIAKVHAPLYSVDVEG
EADTGGQATASQNTATEDAAISEVESESGSDNQISQSVTPGTTIEEFLLPDIGEGIVECELVEWLVSEGDTVVEDQPIAD
VMTDKALVQIPAIKNGKIVKLRVRKGQLAKVHAPLFAIEVEAEVGAGELQAASPLIPESGSSSQSLEATPQGKALASPAV
RRLARSLDLDISTISGSGKNGRVYKEDVERHHANGGQSNISVTQKSVAAPAVSNPAAVIEQPSHRQADRVEPIKGVRAVM
AKMMSESVSTIPHFTYCEEFDLTELVALRESVKKKYSTDELKLTMMPFFMKSMSLALSQFPDMNSRVNGDCTEQTYLASH
NIGMAVDSKVGLLVPNVKDVQDKTILQIAAEITRLTTAARSGRVSPNDLKGGSISISNIGALGGTVATPIINKPEVAIVA
LGKLQVLPRFNDKGEVEARKIMQVSWSGDHRVIDGGTIARFCNLWKLYLEQPQEMLLAMQ
>Mature_540_residues
MIKEFILPDIGEGVVECELVEWLVQEGDSVVEDQPIADVMTDKALVQIPAPNAGVITKLYYAKGEIAKVHAPLYSVDVEG
EADTGGQATASQNTATEDAAISEVESESGSDNQISQSVTPGTTIEEFLLPDIGEGIVECELVEWLVSEGDTVVEDQPIAD
VMTDKALVQIPAIKNGKIVKLRVRKGQLAKVHAPLFAIEVEAEVGAGELQAASPLIPESGSSSQSLEATPQGKALASPAV
RRLARSLDLDISTISGSGKNGRVYKEDVERHHANGGQSNISVTQKSVAAPAVSNPAAVIEQPSHRQADRVEPIKGVRAVM
AKMMSESVSTIPHFTYCEEFDLTELVALRESVKKKYSTDELKLTMMPFFMKSMSLALSQFPDMNSRVNGDCTEQTYLASH
NIGMAVDSKVGLLVPNVKDVQDKTILQIAAEITRLTTAARSGRVSPNDLKGGSISISNIGALGGTVATPIINKPEVAIVA
LGKLQVLPRFNDKGEVEARKIMQVSWSGDHRVIDGGTIARFCNLWKLYLEQPQEMLLAMQ

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=421, Percent_Identity=44.8931116389549, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI31711992, Length=565, Percent_Identity=24.4247787610619, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI203098816, Length=452, Percent_Identity=26.7699115044248, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI203098753, Length=446, Percent_Identity=26.6816143497758, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI19923748, Length=261, Percent_Identity=29.8850574712644, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI260898739, Length=158, Percent_Identity=34.8101265822785, Blast_Score=92, Evalue=2e-18,
Organism=Escherichia coli, GI1786305, Length=556, Percent_Identity=29.3165467625899, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1786946, Length=416, Percent_Identity=28.3653846153846, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17537937, Length=426, Percent_Identity=38.9671361502347, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI25146366, Length=415, Percent_Identity=29.1566265060241, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17560088, Length=451, Percent_Identity=28.3813747228381, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=332, Percent_Identity=28.9156626506024, Blast_Score=105, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6320352, Length=420, Percent_Identity=28.8095238095238, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6324258, Length=445, Percent_Identity=25.1685393258427, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI18859875, Length=426, Percent_Identity=40.6103286384976, Blast_Score=289, Evalue=3e-78,
Organism=Drosophila melanogaster, GI24645909, Length=232, Percent_Identity=29.7413793103448, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 57994; Mature: 57994

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKEFILPDIGEGVVECELVEWLVQEGDSVVEDQPIADVMTDKALVQIPAPNAGVITKLY
CCCHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCEEEEECCCCCCCEEEEE
YAKGEIAKVHAPLYSVDVEGEADTGGQATASQNTATEDAAISEVESESGSDNQISQSVTP
ECCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHCCCCC
GTTIEEFLLPDIGEGIVECELVEWLVSEGDTVVEDQPIADVMTDKALVQIPAIKNGKIVK
CCCHHHHHCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHCCCCEEEECCCCCCCEEE
LRVRKGQLAKVHAPLFAIEVEAEVGAGELQAASPLIPESGSSSQSLEATPQGKALASPAV
EEECCCCEEEEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RRLARSLDLDISTISGSGKNGRVYKEDVERHHANGGQSNISVTQKSVAAPAVSNPAAVIE
HHHHHHHCCEEEEECCCCCCCCEEHHHHHHHHCCCCCCCCEEHHHHHCCCCCCCCHHHHC
QPSHRQADRVEPIKGVRAVMAKMMSESVSTIPHFTYCEEFDLTELVALRESVKKKYSTDE
CCCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
LKLTMMPFFMKSMSLALSQFPDMNSRVNGDCTEQTYLASHNIGMAVDSKVGLLVPNVKDV
EEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCEEECCCCCEECCCCCCC
QDKTILQIAAEITRLTTAARSGRVSPNDLKGGSISISNIGALGGTVATPIINKPEVAIVA
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCEEEEE
LGKLQVLPRFNDKGEVEARKIMQVSWSGDHRVIDGGTIARFCNLWKLYLEQPQEMLLAMQ
ECCEEEECCCCCCCCCCEEEEEEEEECCCEEEECCCHHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MIKEFILPDIGEGVVECELVEWLVQEGDSVVEDQPIADVMTDKALVQIPAPNAGVITKLY
CCCHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCEEEEECCCCCCCEEEEE
YAKGEIAKVHAPLYSVDVEGEADTGGQATASQNTATEDAAISEVESESGSDNQISQSVTP
ECCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHCCCCC
GTTIEEFLLPDIGEGIVECELVEWLVSEGDTVVEDQPIADVMTDKALVQIPAIKNGKIVK
CCCHHHHHCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHCCCCEEEECCCCCCCEEE
LRVRKGQLAKVHAPLFAIEVEAEVGAGELQAASPLIPESGSSSQSLEATPQGKALASPAV
EEECCCCEEEEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RRLARSLDLDISTISGSGKNGRVYKEDVERHHANGGQSNISVTQKSVAAPAVSNPAAVIE
HHHHHHHCCEEEEECCCCCCCCEEHHHHHHHHCCCCCCCCEEHHHHHCCCCCCCCHHHHC
QPSHRQADRVEPIKGVRAVMAKMMSESVSTIPHFTYCEEFDLTELVALRESVKKKYSTDE
CCCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
LKLTMMPFFMKSMSLALSQFPDMNSRVNGDCTEQTYLASHNIGMAVDSKVGLLVPNVKDV
EEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCEEECCCCCEECCCCCCC
QDKTILQIAAEITRLTTAARSGRVSPNDLKGGSISISNIGALGGTVATPIINKPEVAIVA
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCEEEEE
LGKLQVLPRFNDKGEVEARKIMQVSWSGDHRVIDGGTIARFCNLWKLYLEQPQEMLLAMQ
ECCEEEECCCCCCCCCCEEEEEEEEECCCEEEECCCHHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]