Click here to switch to the map view.

The map label for this gene is paaG [C]

Identifier: 157961992

GI number: 157961992

Start: 2627940

End: 2628788

Strand: Direct

Name: paaG [C]

Synonym: Spea_2171

Alternate gene names: 157961992

Gene position: 2627940-2628788 (Clockwise)

Preceding gene: 157961991

Following gene: 157961993

Centisome position: 50.79

GC content: 44.99

Gene sequence:

>849_bases
ATGTCAGTAATTTTAGAAAAAGCACAGAAACTATTCTTGTCAGACAATGACGCAAATGTACTCACAGGCCATCAATTTGA
AACGGTTAATTATTTAGTCAAAGACAAAATAGGCATTATCGAGCTAAATCGACCCAATAAAATGAATGCTTTCAATCAAA
CCATGCGCAGCGAGCTGCAGGCTGCGATAGAAATGGCAGACAATAATGACGATTTAAGAGTGATTATGATCCGTGGTGCT
GGTGATCACTTCTCTTCGGGCGCGGACTTGAAAGAGCTTGTTGGTGCCAAGCACGGTATAGAGGCACAGATCCTTAATGA
ATATAAGCCATTTTTATCACGTATTGCCCAGTCAAAAAAGATTTATATGGCAGTTGTACAAGGCGCCGCAGCAGGGATAG
GCGGTTCGTTAGCCTTAAATTGTGACTTAGTCGTTATGGCTGAAAGCGCCTGCCTTTTTCAGGCTTTTGCCGCTATTGGA
TTAGTGCCTGATGGAGGCGCAAGCTGGCATTTAGTCAATAAACTGGGCTACAAGAAAGCGTTCGAACTTTGCGTCGAGGC
CGACAAGCTCACGGCGCTGGAGTGTCTGAGCAGCGGCTTGGTCAATCGTATTTCTGATGATGCAAGTGTGCAAGGTGATG
CACTTGTTTGGGCACAAAAATTGGCCACCGGAGCACCACTATCACAGCAGTACTTAAAGCGATTATTGCAACAAGCTCAG
CGAGGGAGCTTACACGACACCATTCGCCAAGAGGCGCTGTATCAGCAGTTCTGCTTCAACAGTGACGATTTTAAAGAAGG
TGTGAATGCCTTTTTTGAAAAACGCGATGCAACCTTTACTGGTAAGTAG

Upstream 100 bases:

>100_bases
GGCGTCGAGGCGTTAAATACGTTGTTGTAGCAATGTGTATTGGCGGTGGTATGGGCGCAGCAGGACTCTTTGAAGTCAAT
CAGTAATAAAGGAGTGATTC

Downstream 100 bases:

>100_bases
CTGATATTAACCCGAATTAAAGCAATAGATCAGATTATTAAGAACGTTATTAATGGGAGACGTCATGCGCGAAGTCATGG
ATTTTGATGTTGTGATTGTA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSVILEKAQKLFLSDNDANVLTGHQFETVNYLVKDKIGIIELNRPNKMNAFNQTMRSELQAAIEMADNNDDLRVIMIRGA
GDHFSSGADLKELVGAKHGIEAQILNEYKPFLSRIAQSKKIYMAVVQGAAAGIGGSLALNCDLVVMAESACLFQAFAAIG
LVPDGGASWHLVNKLGYKKAFELCVEADKLTALECLSSGLVNRISDDASVQGDALVWAQKLATGAPLSQQYLKRLLQQAQ
RGSLHDTIRQEALYQQFCFNSDDFKEGVNAFFEKRDATFTGK

Sequences:

>Translated_282_residues
MSVILEKAQKLFLSDNDANVLTGHQFETVNYLVKDKIGIIELNRPNKMNAFNQTMRSELQAAIEMADNNDDLRVIMIRGA
GDHFSSGADLKELVGAKHGIEAQILNEYKPFLSRIAQSKKIYMAVVQGAAAGIGGSLALNCDLVVMAESACLFQAFAAIG
LVPDGGASWHLVNKLGYKKAFELCVEADKLTALECLSSGLVNRISDDASVQGDALVWAQKLATGAPLSQQYLKRLLQQAQ
RGSLHDTIRQEALYQQFCFNSDDFKEGVNAFFEKRDATFTGK
>Mature_281_residues
SVILEKAQKLFLSDNDANVLTGHQFETVNYLVKDKIGIIELNRPNKMNAFNQTMRSELQAAIEMADNNDDLRVIMIRGAG
DHFSSGADLKELVGAKHGIEAQILNEYKPFLSRIAQSKKIYMAVVQGAAAGIGGSLALNCDLVVMAESACLFQAFAAIGL
VPDGGASWHLVNKLGYKKAFELCVEADKLTALECLSSGLVNRISDDASVQGDALVWAQKLATGAPLSQQYLKRLLQQAQR
GSLHDTIRQEALYQQFCFNSDDFKEGVNAFFEKRDATFTGK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=266, Percent_Identity=28.5714285714286, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI45643119, Length=254, Percent_Identity=28.3464566929134, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI260275230, Length=254, Percent_Identity=28.3464566929134, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI260274832, Length=254, Percent_Identity=28.3464566929134, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI157694516, Length=221, Percent_Identity=27.6018099547511, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI213417737, Length=221, Percent_Identity=27.6018099547511, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI70995211, Length=267, Percent_Identity=26.9662921348315, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI31542718, Length=256, Percent_Identity=25.390625, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI25453481, Length=213, Percent_Identity=26.7605633802817, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI51538573, Length=213, Percent_Identity=26.7605633802817, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4757966, Length=213, Percent_Identity=26.7605633802817, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI51538581, Length=213, Percent_Identity=26.7605633802817, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI116235487, Length=228, Percent_Identity=25.4385964912281, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI4757968, Length=199, Percent_Identity=26.6331658291457, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI49355787, Length=199, Percent_Identity=26.6331658291457, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI221307494, Length=193, Percent_Identity=25.3886010362694, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI221136756, Length=193, Percent_Identity=25.3886010362694, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI221136753, Length=193, Percent_Identity=25.3886010362694, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI157694520, Length=199, Percent_Identity=26.1306532663317, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787660, Length=254, Percent_Identity=31.496062992126, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787659, Length=250, Percent_Identity=30.8, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI221142681, Length=252, Percent_Identity=30.952380952381, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=28.6259541984733, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI87082183, Length=264, Percent_Identity=25.7575757575758, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25145438, Length=246, Percent_Identity=30.8943089430894, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=30.7392996108949, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17535521, Length=248, Percent_Identity=26.2096774193548, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=249, Percent_Identity=27.3092369477912, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=258, Percent_Identity=25.5813953488372, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560910, Length=203, Percent_Identity=29.064039408867, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=198, Percent_Identity=25.7575757575758, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17536985, Length=256, Percent_Identity=24.609375, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=27.8481012658228, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323314, Length=178, Percent_Identity=26.9662921348315, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20129971, Length=246, Percent_Identity=30.4878048780488, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24653477, Length=246, Percent_Identity=30.4878048780488, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24654903, Length=247, Percent_Identity=26.3157894736842, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19920382, Length=251, Percent_Identity=27.0916334661355, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=27.3809523809524, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21357171, Length=265, Percent_Identity=29.811320754717, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=27.3076923076923, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30870; Mature: 30738

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVILEKAQKLFLSDNDANVLTGHQFETVNYLVKDKIGIIELNRPNKMNAFNQTMRSELQ
CCHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHH
AAIEMADNNDDLRVIMIRGAGDHFSSGADLKELVGAKHGIEAQILNEYKPFLSRIAQSKK
HHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCH
IYMAVVQGAAAGIGGSLALNCDLVVMAESACLFQAFAAIGLVPDGGASWHLVNKLGYKKA
HHHHHHHHHHCCCCCCEEEECEEEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
FELCVEADKLTALECLSSGLVNRISDDASVQGDALVWAQKLATGAPLSQQYLKRLLQQAQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
RGSLHDTIRQEALYQQFCFNSDDFKEGVNAFFEKRDATFTGK
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SVILEKAQKLFLSDNDANVLTGHQFETVNYLVKDKIGIIELNRPNKMNAFNQTMRSELQ
CHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHH
AAIEMADNNDDLRVIMIRGAGDHFSSGADLKELVGAKHGIEAQILNEYKPFLSRIAQSKK
HHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCH
IYMAVVQGAAAGIGGSLALNCDLVVMAESACLFQAFAAIGLVPDGGASWHLVNKLGYKKA
HHHHHHHHHHCCCCCCEEEECEEEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
FELCVEADKLTALECLSSGLVNRISDDASVQGDALVWAQKLATGAPLSQQYLKRLLQQAQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
RGSLHDTIRQEALYQQFCFNSDDFKEGVNAFFEKRDATFTGK
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA