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The map label for this gene is paaF [C]

Identifier: 157961977

GI number: 157961977

Start: 2610086

End: 2611042

Strand: Direct

Name: paaF [C]

Synonym: Spea_2156

Alternate gene names: 157961977

Gene position: 2610086-2611042 (Clockwise)

Preceding gene: 157961976

Following gene: 157961978

Centisome position: 50.44

GC content: 47.13

Gene sequence:

>957_bases
ATGTCTAACAATACAGCTCACAGTGAAGCGGCCCCGCAAACTACGGATAACCAACTAGCCGCGCTTGATGTAAGTCAGCG
CGAAGATATTGATGGCGTCGTGTTTTATGAGGTGCGGGAACAAGTTGCAATTGTAACCATGAATCGCCCCGAATATAACA
ATGCACAAAATTCGAAAATGACCTACGCGCTCGACGCCGCTTTTAAACGAGCGTGCGACGACGACAAGGTAAAAGTGATC
ATACTCGCCGGTGAAGGTAAGCACTTTTCAGCCGGACATGACATCGGCACCCCCGGACGCGACGTTGACCAAGAGTTTGA
TCGTGCCAGTCTTTGGTACGATCACAGCAATAAAAGCGGCGGTGAGTTTTTATATGCGAGAGAGCAAGAGGTTTACTTAG
GTATGTGTCGTCGCTGGCGTGATATGCCGAAGCCGACCATTGCTATGGTACAAGGCGCCTGTATCGCTGGCGGGCTAATG
CTAGCCTGGGTATGTGACCTAATTATCTGTTCTGATGATGCCTATTACTGCGACCCAGTTGTTCGAATGGGGATCCCAGG
CGTTGAATATTTTGCGCACGTGCATGAGCTTAACTCTCGCATTGCAAAAGAGTTTTTATTCTTAGGCGACAAAATGAATG
CTCAGCGTGCTTATGAGTTAGGCATGGTGAACCGAATTTTTTCGAGGAAAGATCTCACTAGCGAGACGTTAACCATAGCT
AATAAAATCGCCCAAATGCCACGACTTGGTTTGCAACTAACTAAGCAAGCGATTAATAACGCAGAAGATTTGATGGGCAA
ACGTAGCACTATGGATATGGTTTTTGGCTTACATCATTTTGCTCATGTTCATAATGAGTCAATATCCGGAGACAAACTCG
GTGGCTTTGATGCAAAAGCGATGGCAAAAGCCAACAAGGCTGCCAGCGGTGCAGAGAAGTGCAAGGAGACATCATGA

Upstream 100 bases:

>100_bases
TTGCCGCGTTAGATCCGCATAACTTAAGAGCAAAACAACTTAAAGACAATCCTCCTGGTGATAGAACCACTAGCGCCAAA
CTCGTAGAAGAGGTGGCCTA

Downstream 100 bases:

>100_bases
GCCATGCCGTTGATGCTACAAATCCTGTTGATCCAGTGTATGAGCTTAAAACCGATTTAACTGAAATGCTGGGTTGCCGC
TATCCCATTGTCCAAACCGC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSNNTAHSEAAPQTTDNQLAALDVSQREDIDGVVFYEVREQVAIVTMNRPEYNNAQNSKMTYALDAAFKRACDDDKVKVI
ILAGEGKHFSAGHDIGTPGRDVDQEFDRASLWYDHSNKSGGEFLYAREQEVYLGMCRRWRDMPKPTIAMVQGACIAGGLM
LAWVCDLIICSDDAYYCDPVVRMGIPGVEYFAHVHELNSRIAKEFLFLGDKMNAQRAYELGMVNRIFSRKDLTSETLTIA
NKIAQMPRLGLQLTKQAINNAEDLMGKRSTMDMVFGLHHFAHVHNESISGDKLGGFDAKAMAKANKAASGAEKCKETS

Sequences:

>Translated_318_residues
MSNNTAHSEAAPQTTDNQLAALDVSQREDIDGVVFYEVREQVAIVTMNRPEYNNAQNSKMTYALDAAFKRACDDDKVKVI
ILAGEGKHFSAGHDIGTPGRDVDQEFDRASLWYDHSNKSGGEFLYAREQEVYLGMCRRWRDMPKPTIAMVQGACIAGGLM
LAWVCDLIICSDDAYYCDPVVRMGIPGVEYFAHVHELNSRIAKEFLFLGDKMNAQRAYELGMVNRIFSRKDLTSETLTIA
NKIAQMPRLGLQLTKQAINNAEDLMGKRSTMDMVFGLHHFAHVHNESISGDKLGGFDAKAMAKANKAASGAEKCKETS
>Mature_317_residues
SNNTAHSEAAPQTTDNQLAALDVSQREDIDGVVFYEVREQVAIVTMNRPEYNNAQNSKMTYALDAAFKRACDDDKVKVII
LAGEGKHFSAGHDIGTPGRDVDQEFDRASLWYDHSNKSGGEFLYAREQEVYLGMCRRWRDMPKPTIAMVQGACIAGGLML
AWVCDLIICSDDAYYCDPVVRMGIPGVEYFAHVHELNSRIAKEFLFLGDKMNAQRAYELGMVNRIFSRKDLTSETLTIAN
KIAQMPRLGLQLTKQAINNAEDLMGKRSTMDMVFGLHHFAHVHNESISGDKLGGFDAKAMAKANKAASGAEKCKETS

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=215, Percent_Identity=28.8372093023256, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI194097323, Length=225, Percent_Identity=28, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI70995211, Length=201, Percent_Identity=29.3532338308458, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI45643119, Length=227, Percent_Identity=25.9911894273128, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260275230, Length=227, Percent_Identity=25.9911894273128, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260274832, Length=227, Percent_Identity=25.9911894273128, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1787659, Length=229, Percent_Identity=27.9475982532751, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788597, Length=237, Percent_Identity=28.6919831223629, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI221142681, Length=251, Percent_Identity=26.6932270916335, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787660, Length=231, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082183, Length=243, Percent_Identity=25.5144032921811, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=224, Percent_Identity=29.4642857142857, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=233, Percent_Identity=28.755364806867, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=231, Percent_Identity=26.4069264069264, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=227, Percent_Identity=27.3127753303965, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=219, Percent_Identity=26.9406392694064, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=27.5303643724696, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=27.5303643724696, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19920382, Length=218, Percent_Identity=27.5229357798165, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24650670, Length=263, Percent_Identity=26.9961977186312, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357171, Length=222, Percent_Identity=27.4774774774775, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24654903, Length=217, Percent_Identity=22.1198156682028, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 35331; Mature: 35200

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNTAHSEAAPQTTDNQLAALDVSQREDIDGVVFYEVREQVAIVTMNRPEYNNAQNSKM
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCEE
TYALDAAFKRACDDDKVKVIILAGEGKHFSAGHDIGTPGRDVDQEFDRASLWYDHSNKSG
EEHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCCCC
GEFLYAREQEVYLGMCRRWRDMPKPTIAMVQGACIAGGLMLAWVCDLIICSDDAYYCDPV
CCEEEECCHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHHHHEECCCCEECCHH
VRMGIPGVEYFAHVHELNSRIAKEFLFLGDKMNAQRAYELGMVNRIFSRKDLTSETLTIA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
NKIAQMPRLGLQLTKQAINNAEDLMGKRSTMDMVFGLHHFAHVHNESISGDKLGGFDAKA
HHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
MAKANKAASGAEKCKETS
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SNNTAHSEAAPQTTDNQLAALDVSQREDIDGVVFYEVREQVAIVTMNRPEYNNAQNSKM
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCEE
TYALDAAFKRACDDDKVKVIILAGEGKHFSAGHDIGTPGRDVDQEFDRASLWYDHSNKSG
EEHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCCCC
GEFLYAREQEVYLGMCRRWRDMPKPTIAMVQGACIAGGLMLAWVCDLIICSDDAYYCDPV
CCEEEECCHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHHHHEECCCCEECCHH
VRMGIPGVEYFAHVHELNSRIAKEFLFLGDKMNAQRAYELGMVNRIFSRKDLTSETLTIA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
NKIAQMPRLGLQLTKQAINNAEDLMGKRSTMDMVFGLHHFAHVHNESISGDKLGGFDAKA
HHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
MAKANKAASGAEKCKETS
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA