Click here to switch to the map view.

The map label for this gene is csgA [H]

Identifier: 157961921

GI number: 157961921

Start: 2542979

End: 2543674

Strand: Direct

Name: csgA [H]

Synonym: Spea_2100

Alternate gene names: 157961921

Gene position: 2542979-2543674 (Clockwise)

Preceding gene: 157961918

Following gene: 157961922

Centisome position: 49.14

GC content: 42.1

Gene sequence:

>696_bases
ATGACTAAACACGCATTGATTACGGGTGGTAATCGTGGAATTGGACGAGCGTTCGTAGAACACTATTTAAAAGCTGGTTG
GAATGTTACAGCTTGTTGTCGAGATCCTAAAAGGGCGGTTGAACTGTCGGTATTAAAATCTGATTATGAGCAACTAAAAG
TTATGTCACTGGACGTCAGCCTTTCAGAGTCAATTGCTATCTTAACCAAAGAATTGGCCGGAACTCCGATCGATTTACTA
ATTAATAATGCAGGGTATTATGGTCCTAAGGGAGTCGAGTTTGGAAGCTGTGATGCTAAGGAGTGGGGTAAAGTCATTGA
GGTTAACACTATTGCACCATTGATGCTTACAGAAGCCTTGTATCAAAACCTAAAATTAGTGGGCAACCCTGTAGTCGCCT
TTATTTCCTCTAAAGTCGGCAGTATGGAAGATAATACCAGTGGTGGTGGCTATTACTATCGCTCATCCAAAGCGGCGCTT
AATTCTGTGGTAAAGAGTTTATCTATTGACTTGAAAGATGACGGAATAAAATGTGTTGCTCTGCATCCAGGTTGGGTACT
GACAGCAATGGGAGGCCCTAAAGCACTTATCGATACCGACATGTCGGTTAAGGGCATGATGGCAGTAATAGGTAAGTTAA
CATGGGAACAAAGTGGTGATTTTTACGACTACCAAGGAAAACCAATACCTTGGTAG

Upstream 100 bases:

>100_bases
TTTCATGTTGAGTCGTTTTTGTTAAATAATCGCCAACTTCTTGCAACACTGAACTAGACTGAGCGAATATAAGGCGATGA
AGCTTAGTGAAGGTAACCAA

Downstream 100 bases:

>100_bases
TTTCAATTTGGGTGGGACTCGGTGGTTGAGTTTAGGGGAAACTGGCATTTTAAATAGCGTAAATTATAATTAACTCGACT
AATCTACTCAGAAAACAGGA

Product: short-chain dehydrogenase/reductase SDR

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: C signal [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MTKHALITGGNRGIGRAFVEHYLKAGWNVTACCRDPKRAVELSVLKSDYEQLKVMSLDVSLSESIAILTKELAGTPIDLL
INNAGYYGPKGVEFGSCDAKEWGKVIEVNTIAPLMLTEALYQNLKLVGNPVVAFISSKVGSMEDNTSGGGYYYRSSKAAL
NSVVKSLSIDLKDDGIKCVALHPGWVLTAMGGPKALIDTDMSVKGMMAVIGKLTWEQSGDFYDYQGKPIPW

Sequences:

>Translated_231_residues
MTKHALITGGNRGIGRAFVEHYLKAGWNVTACCRDPKRAVELSVLKSDYEQLKVMSLDVSLSESIAILTKELAGTPIDLL
INNAGYYGPKGVEFGSCDAKEWGKVIEVNTIAPLMLTEALYQNLKLVGNPVVAFISSKVGSMEDNTSGGGYYYRSSKAAL
NSVVKSLSIDLKDDGIKCVALHPGWVLTAMGGPKALIDTDMSVKGMMAVIGKLTWEQSGDFYDYQGKPIPW
>Mature_230_residues
TKHALITGGNRGIGRAFVEHYLKAGWNVTACCRDPKRAVELSVLKSDYEQLKVMSLDVSLSESIAILTKELAGTPIDLLI
NNAGYYGPKGVEFGSCDAKEWGKVIEVNTIAPLMLTEALYQNLKLVGNPVVAFISSKVGSMEDNTSGGGYYYRSSKAALN
SVVKSLSIDLKDDGIKCVALHPGWVLTAMGGPKALIDTDMSVKGMMAVIGKLTWEQSGDFYDYQGKPIPW

Specific function: Cell-cell signaling protein required for fruiting body morphogenesis. Necessary for cellular aggregation, for spore differentiation, and for gene expression that is initiated after 6 hour of starvation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To yeast YKL071W [H]

Homologues:

Organism=Escherichia coli, GI1787335, Length=202, Percent_Identity=30.1980198019802, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17532177, Length=248, Percent_Identity=32.6612903225806, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17558990, Length=254, Percent_Identity=32.6771653543307, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17558994, Length=253, Percent_Identity=32.0158102766798, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17559964, Length=248, Percent_Identity=34.6774193548387, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17558996, Length=251, Percent_Identity=32.2709163346614, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17558992, Length=250, Percent_Identity=32.4, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI193207563, Length=250, Percent_Identity=29.2, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17559962, Length=251, Percent_Identity=31.4741035856574, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI72001658, Length=241, Percent_Identity=32.7800829875519, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17541382, Length=256, Percent_Identity=29.6875, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17542178, Length=239, Percent_Identity=28.0334728033473, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=213, Percent_Identity=29.5774647887324, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=205, Percent_Identity=30.2439024390244, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538486, Length=210, Percent_Identity=29.5238095238095, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322779, Length=254, Percent_Identity=28.740157480315, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640547, Length=244, Percent_Identity=35.2459016393443, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24643142, Length=189, Percent_Identity=32.2751322751323, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 24988; Mature: 24857

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKHALITGGNRGIGRAFVEHYLKAGWNVTACCRDPKRAVELSVLKSDYEQLKVMSLDVS
CCCCEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHEEEEEECCC
LSESIAILTKELAGTPIDLLINNAGYYGPKGVEFGSCDAKEWGKVIEVNTIAPLMLTEAL
CCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCEEEECCCHHHHHHHHH
YQNLKLVGNPVVAFISSKVGSMEDNTSGGGYYYRSSKAALNSVVKSLSIDLKDDGIKCVA
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCEECCCCEEEEE
LHPGWVLTAMGGPKALIDTDMSVKGMMAVIGKLTWEQSGDFYDYQGKPIPW
ECCCEEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCC
>Mature Secondary Structure 
TKHALITGGNRGIGRAFVEHYLKAGWNVTACCRDPKRAVELSVLKSDYEQLKVMSLDVS
CCCEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHEEEEEECCC
LSESIAILTKELAGTPIDLLINNAGYYGPKGVEFGSCDAKEWGKVIEVNTIAPLMLTEAL
CCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCEEEECCCHHHHHHHHH
YQNLKLVGNPVVAFISSKVGSMEDNTSGGGYYYRSSKAALNSVVKSLSIDLKDDGIKCVA
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCEECCCCEEEEE
LHPGWVLTAMGGPKALIDTDMSVKGMMAVIGKLTWEQSGDFYDYQGKPIPW
ECCCEEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2152896; 2107980; 2118510; 1372277 [H]