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The map label for this gene is rimK [H]

Identifier: 157961922

GI number: 157961922

Start: 2543977

End: 2545353

Strand: Direct

Name: rimK [H]

Synonym: Spea_2101

Alternate gene names: 157961922

Gene position: 2543977-2545353 (Clockwise)

Preceding gene: 157961921

Following gene: 157961923

Centisome position: 49.16

GC content: 43.06

Gene sequence:

>1377_bases
ATGAGCGACAATAAGTTTATTATCGGAAGTGAAGAGTGGTGTGCATTCCCAGCTCTTGGTGTTCCTGCAATTAAAGCTAG
AGTAGACTCTGGCGCCAGAACGTCATCAATCCATGCTGTAAATATCCGTCCTTTTAAAAGAGAAGGCGAGCCATGGGTTT
CTTTTGAACTTCATCCGATACAAAACAGTCGCAAGATCATTCTCAGATGTGAGAGTAAGGTTGTTGATCGCCGTAATATT
AAGAGTTCTAACGGTGAATCAGAGAAACGTTATGTTATTGCATCTGTTATCCAGTTAGGTGGTAATGCATGGGAAGTTGA
GTTGACCTTAACTAACCGTGACAGCATGGGCTACCGTATGCTGTTAGGTCGTGAAGCGATGAGCAACAAGACCTTGGTTG
ACCCATCAGAATCATTTTGTTTAGGTGAGAAGGGCGACCTAGAGGTTGAGCAACTTTACGGTGTTAAAACAGCTAAGAAA
AGTGGTCTTAAGATCGGATTGCTAGCCAGTAACCCAGACTTGTACAGTAACCGCCGTATCATTGAAGCCGGTGAACAGCG
CGGCCACGAAATGGTATTCTTAAATATCAGCCAATGTTACTTGAAGCTAGATGCGTCTTCGCCAGAAGTTCACTATCGTG
GTGGCAGAGTTCTTAATAACGTTGATGCGGTTATTCCACGTATTAGACCAAGCATGACCTTCTACGGTTGCGCGTTGACT
CGTCACTTTGAAAGTTTGAATATCGAAGCGTTAAACACTTCTGATGCGATTACTCGCTCAAGAGATAAGCTGTTTTCGCT
GCAGTTATTGCAAAAGAATAACCTAGATATTCCAACGAGTGGTTTTGCTAACTCGCCTGTTGATACTAAAGATCTTATCG
ATATGGTTGGCGGCGCTCCGCTTATCGTTAAGTTGCTGGAAGGTACACAAGGTAAGGGCGTTGTCCTAGCGGAAACAACT
AAAGCAGCAGAGAGTGTAATTAACGCATTTAAATCTCTGCGTGTTAACTTACTTGTGCAAGAGTTTATTAAAGAAGCTCA
AGGTAAAGACTTACGCTGTTTTGTAATTGACGGTAAGATTGTTGCGTCAATTGAGCGTACAGCCGCTCCTGGTGAGTTCA
GAGCTAACATTCATATGGGTGGTAGTGCATCAATCGTTTCAGTGAGTGCTGCAGAAAAACAATTGGCGATTAAAGCAGCC
AAAACTATGGGTTTACGTGTTGCCGGTGTTGATATTATTCGTTCTAGCCGAGGACCTCTATTACTAGAGATAAACTCTTC
ACCAGGACTTGAAGGTATTGAATCTGCTACAGGTATTGATATCGCTGGTGCGATGATCGAATCTATCGAGAAGAAACTAG
GTTGGAAGGCTGATTAA

Upstream 100 bases:

>100_bases
ATTCTATCCGTAAGCAGAAAATAACTAAAATTTAGTTTGCATCTGCTGAAAAAAGGAGTACTTTCGCGCTCCTTAATTTA
ACTTAACTGGAAGAATTGTA

Downstream 100 bases:

>100_bases
CAGCGTCTAATTCTAGGTTAGCTCTAAAAAGCGTTATGTCTAAGACATAGCGCTTTTTTTTGTTTAATTGTCGCTTGCAC
AAGCTAGTTAAGTTTTTATT

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSDNKFIIGSEEWCAFPALGVPAIKARVDSGARTSSIHAVNIRPFKREGEPWVSFELHPIQNSRKIILRCESKVVDRRNI
KSSNGESEKRYVIASVIQLGGNAWEVELTLTNRDSMGYRMLLGREAMSNKTLVDPSESFCLGEKGDLEVEQLYGVKTAKK
SGLKIGLLASNPDLYSNRRIIEAGEQRGHEMVFLNISQCYLKLDASSPEVHYRGGRVLNNVDAVIPRIRPSMTFYGCALT
RHFESLNIEALNTSDAITRSRDKLFSLQLLQKNNLDIPTSGFANSPVDTKDLIDMVGGAPLIVKLLEGTQGKGVVLAETT
KAAESVINAFKSLRVNLLVQEFIKEAQGKDLRCFVIDGKIVASIERTAAPGEFRANIHMGGSASIVSVSAAEKQLAIKAA
KTMGLRVAGVDIIRSSRGPLLLEINSSPGLEGIESATGIDIAGAMIESIEKKLGWKAD

Sequences:

>Translated_458_residues
MSDNKFIIGSEEWCAFPALGVPAIKARVDSGARTSSIHAVNIRPFKREGEPWVSFELHPIQNSRKIILRCESKVVDRRNI
KSSNGESEKRYVIASVIQLGGNAWEVELTLTNRDSMGYRMLLGREAMSNKTLVDPSESFCLGEKGDLEVEQLYGVKTAKK
SGLKIGLLASNPDLYSNRRIIEAGEQRGHEMVFLNISQCYLKLDASSPEVHYRGGRVLNNVDAVIPRIRPSMTFYGCALT
RHFESLNIEALNTSDAITRSRDKLFSLQLLQKNNLDIPTSGFANSPVDTKDLIDMVGGAPLIVKLLEGTQGKGVVLAETT
KAAESVINAFKSLRVNLLVQEFIKEAQGKDLRCFVIDGKIVASIERTAAPGEFRANIHMGGSASIVSVSAAEKQLAIKAA
KTMGLRVAGVDIIRSSRGPLLLEINSSPGLEGIESATGIDIAGAMIESIEKKLGWKAD
>Mature_457_residues
SDNKFIIGSEEWCAFPALGVPAIKARVDSGARTSSIHAVNIRPFKREGEPWVSFELHPIQNSRKIILRCESKVVDRRNIK
SSNGESEKRYVIASVIQLGGNAWEVELTLTNRDSMGYRMLLGREAMSNKTLVDPSESFCLGEKGDLEVEQLYGVKTAKKS
GLKIGLLASNPDLYSNRRIIEAGEQRGHEMVFLNISQCYLKLDASSPEVHYRGGRVLNNVDAVIPRIRPSMTFYGCALTR
HFESLNIEALNTSDAITRSRDKLFSLQLLQKNNLDIPTSGFANSPVDTKDLIDMVGGAPLIVKLLEGTQGKGVVLAETTK
AAESVINAFKSLRVNLLVQEFIKEAQGKDLRCFVIDGKIVASIERTAAPGEFRANIHMGGSASIVSVSAAEKQLAIKAAK
TMGLRVAGVDIIRSSRGPLLLEINSSPGLEGIESATGIDIAGAMIESIEKKLGWKAD

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=206, Percent_Identity=26.6990291262136, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI134254462, Length=206, Percent_Identity=27.1844660194175, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1787076, Length=289, Percent_Identity=57.0934256055363, Blast_Score=338, Evalue=3e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008503
- InterPro:   IPR009007
- InterPro:   IPR004666 [H]

Pfam domain/function: PF05618 DUF785; PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 49928; Mature: 49797

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNKFIIGSEEWCAFPALGVPAIKARVDSGARTSSIHAVNIRPFKREGEPWVSFELHPI
CCCCEEEEECCCCCCCCCCCCCHHEEECCCCCCCCEEEEEEEEEECCCCCCEEEEEEEEC
QNSRKIILRCESKVVDRRNIKSSNGESEKRYVIASVIQLGGNAWEVELTLTNRDSMGYRM
CCCCEEEEEECCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCEEE
LLGREAMSNKTLVDPSESFCLGEKGDLEVEQLYGVKTAKKSGLKIGLLASNPDLYSNRRI
EECCHHHCCCEEECCCCCEECCCCCCCCHHHHHCCHHHHCCCCEEEEEECCCCCCCCCEE
IEAGEQRGHEMVFLNISQCYLKLDASSPEVHYRGGRVLNNVDAVIPRIRPSMTFYGCALT
EECHHHCCCEEEEEEEEEEEEEECCCCCCEEECCCCEECCHHHHHCCCCCCCEEEEHHHH
RHFESLNIEALNTSDAITRSRDKLFSLQLLQKNNLDIPTSGFANSPVDTKDLIDMVGGAP
HHHHHCEEEEECCCHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCC
LIVKLLEGTQGKGVVLAETTKAAESVINAFKSLRVNLLVQEFIKEAQGKDLRCFVIDGKI
EEHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEE
VASIERTAAPGEFRANIHMGGSASIVSVSAAEKQLAIKAAKTMGLRVAGVDIIRSSRGPL
EEEEHHCCCCCCEEEEEEECCCCEEEEEEHHHHHHHHHHHHHCCEEEECEEEEECCCCCE
LLEINSSPGLEGIESATGIDIAGAMIESIEKKLGWKAD
EEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SDNKFIIGSEEWCAFPALGVPAIKARVDSGARTSSIHAVNIRPFKREGEPWVSFELHPI
CCCEEEEECCCCCCCCCCCCCHHEEECCCCCCCCEEEEEEEEEECCCCCCEEEEEEEEC
QNSRKIILRCESKVVDRRNIKSSNGESEKRYVIASVIQLGGNAWEVELTLTNRDSMGYRM
CCCCEEEEEECCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCEEE
LLGREAMSNKTLVDPSESFCLGEKGDLEVEQLYGVKTAKKSGLKIGLLASNPDLYSNRRI
EECCHHHCCCEEECCCCCEECCCCCCCCHHHHHCCHHHHCCCCEEEEEECCCCCCCCCEE
IEAGEQRGHEMVFLNISQCYLKLDASSPEVHYRGGRVLNNVDAVIPRIRPSMTFYGCALT
EECHHHCCCEEEEEEEEEEEEEECCCCCCEEECCCCEECCHHHHHCCCCCCCEEEEHHHH
RHFESLNIEALNTSDAITRSRDKLFSLQLLQKNNLDIPTSGFANSPVDTKDLIDMVGGAP
HHHHHCEEEEECCCHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCC
LIVKLLEGTQGKGVVLAETTKAAESVINAFKSLRVNLLVQEFIKEAQGKDLRCFVIDGKI
EEHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEE
VASIERTAAPGEFRANIHMGGSASIVSVSAAEKQLAIKAAKTMGLRVAGVDIIRSSRGPL
EEEEHHCCCCCCEEEEEEECCCCEEEEEEHHHHHHHHHHHHHCCEEEECEEEEECCCCCE
LLEINSSPGLEGIESATGIDIAGAMIESIEKKLGWKAD
EEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA