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The map label for this gene is 157961698

Identifier: 157961698

GI number: 157961698

Start: 2285111

End: 2286271

Strand: Reverse

Name: 157961698

Synonym: Spea_1874

Alternate gene names: NA

Gene position: 2286271-2285111 (Counterclockwise)

Preceding gene: 157961701

Following gene: 157961697

Centisome position: 44.18

GC content: 46.6

Gene sequence:

>1161_bases
ATGCCATCTTATGTGAAGCAAGGTATTGTGCCCGCTAAGCGCCATATCACCTTTAAAAAAGACAATGGAGAGCTCTACCG
TGAAGAGCTATTCTCCACCCATGGCTTTTCCAACATCTATTCCAATAAGTACCATCACAACATGCCAACCAAGGCATTAG
CAGTGAGCCCCTATAGCTTGTCTCATGGCGAAGACTGGCAAGACTCATTAGTACAAAACTATAAGTTAGATACCAAGCAA
GCTGATTGCGGTGGCAACTTTTTTAGTAGCCGTAACAAGATATTCTTTAATAATGATGTCGCCATGTACACGGCAAAGGT
CAGCGAAGAGTGTGATGAATTCTACCGTAACGCCTACGCAGATGAAGTAGTGTTCGTTCACGAGGGCCAAGGCACCCTTT
ACAGTGAATACGGTGCACTTGCAGTGAAAAAGTGGGACTACTTAGTGATCCCTCGCGGCACCACTTACCAGCTAAAATTT
GATGACTATAGCAATGTCAGATTATTTGTTATCGAGTCTTCATCCATGGTGGAAGTACCAAAGCATTTTCGTAATGAATA
TGGCCAGATGCTTGAATCAGCTCCTTATTGCGAACGTGACATTCGTACACCTGAGCTAGAAGACGCCATCGTAGAGAAAG
GCGACTTCCCGCTTATCTGCAAATTTGGCGATAAGTATCAACTGACATCACTTGAGTGGCACCCATATGATTTAGTGGGC
TGGGACGGCTGTGTGTACCCTTGGGCCTTCAATATTCAAGAGTACGCACCTAAGGTAGGCAAAATCCACCTACCGCCGTC
TGACCACATAGTGTTTAGCGCTCACAACTTTGTGATCTGTAACTTTGTGCCGCGCCCCTATGACTTCCATCCAGAATCGA
TACCCGCACCTTACTATCACAGCAATATCGACAGTGACGAAGTGCTGTATTACGTCGACGGTGACTTTATGAGCCGTACG
GGCATAGAAGCAGGCTTCATGACCCTACACCAAAAAGGTGTGCCTCACGGGCCGCAACCGGGCCGCACGGAGGCCTCAAT
TGGCAAAACAGAAACTTACGAATACGCAGTGATGGTCGATACCTTCGCCCCACTGAAACTAACGGAACATGTAAAGCATT
GCATGAGTAACGACTACAACCGCTCTTGGATTGAAAGCTAA

Upstream 100 bases:

>100_bases
CTAATTATTATTCTTTTTTTTGTTCATTCAAGCAACTAAATTGAACCTAGATCACATTATTATTTTAATGCGTGCGCTTG
AGCGCCGAAGGAAAGCGAAT

Downstream 100 bases:

>100_bases
CAAAACGATTTTTCAAGACTTTAAGCCATCATCAGAAGCCATCGACAGAGTGGCTGGTATGTAGCAACAAATTGACAGAG
GATATAACAATGGCAAGCGA

Product: homogentisate 12-dioxygenase

Products: 4-maleylacetoacetate

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MPSYVKQGIVPAKRHITFKKDNGELYREELFSTHGFSNIYSNKYHHNMPTKALAVSPYSLSHGEDWQDSLVQNYKLDTKQ
ADCGGNFFSSRNKIFFNNDVAMYTAKVSEECDEFYRNAYADEVVFVHEGQGTLYSEYGALAVKKWDYLVIPRGTTYQLKF
DDYSNVRLFVIESSSMVEVPKHFRNEYGQMLESAPYCERDIRTPELEDAIVEKGDFPLICKFGDKYQLTSLEWHPYDLVG
WDGCVYPWAFNIQEYAPKVGKIHLPPSDHIVFSAHNFVICNFVPRPYDFHPESIPAPYYHSNIDSDEVLYYVDGDFMSRT
GIEAGFMTLHQKGVPHGPQPGRTEASIGKTETYEYAVMVDTFAPLKLTEHVKHCMSNDYNRSWIES

Sequences:

>Translated_386_residues
MPSYVKQGIVPAKRHITFKKDNGELYREELFSTHGFSNIYSNKYHHNMPTKALAVSPYSLSHGEDWQDSLVQNYKLDTKQ
ADCGGNFFSSRNKIFFNNDVAMYTAKVSEECDEFYRNAYADEVVFVHEGQGTLYSEYGALAVKKWDYLVIPRGTTYQLKF
DDYSNVRLFVIESSSMVEVPKHFRNEYGQMLESAPYCERDIRTPELEDAIVEKGDFPLICKFGDKYQLTSLEWHPYDLVG
WDGCVYPWAFNIQEYAPKVGKIHLPPSDHIVFSAHNFVICNFVPRPYDFHPESIPAPYYHSNIDSDEVLYYVDGDFMSRT
GIEAGFMTLHQKGVPHGPQPGRTEASIGKTETYEYAVMVDTFAPLKLTEHVKHCMSNDYNRSWIES
>Mature_385_residues
PSYVKQGIVPAKRHITFKKDNGELYREELFSTHGFSNIYSNKYHHNMPTKALAVSPYSLSHGEDWQDSLVQNYKLDTKQA
DCGGNFFSSRNKIFFNNDVAMYTAKVSEECDEFYRNAYADEVVFVHEGQGTLYSEYGALAVKKWDYLVIPRGTTYQLKFD
DYSNVRLFVIESSSMVEVPKHFRNEYGQMLESAPYCERDIRTPELEDAIVEKGDFPLICKFGDKYQLTSLEWHPYDLVGW
DGCVYPWAFNIQEYAPKVGKIHLPPSDHIVFSAHNFVICNFVPRPYDFHPESIPAPYYHSNIDSDEVLYYVDGDFMSRTG
IEAGFMTLHQKGVPHGPQPGRTEASIGKTETYEYAVMVDTFAPLKLTEHVKHCMSNDYNRSWIES

Specific function: Unknown

COG id: COG3508

COG function: function code Q; Homogentisate 1,2-dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the homogentisate dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR005708
- InterPro:   IPR014710 [H]

Pfam domain/function: PF04209 HgmA [H]

EC number: 1.13.11.5

Molecular weight: Translated: 44537; Mature: 44406

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSYVKQGIVPAKRHITFKKDNGELYREELFSTHGFSNIYSNKYHHNMPTKALAVSPYSL
CCCHHHHCCCCCHHEEEEECCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEECCCCC
SHGEDWQDSLVQNYKLDTKQADCGGNFFSSRNKIFFNNDVAMYTAKVSEECDEFYRNAYA
CCCCCHHHHHHHHCCCCCCCCCCCCCHHCCCCEEEECCCEEEEEEHHHHHHHHHHHHCCC
DEVVFVHEGQGTLYSEYGALAVKKWDYLVIPRGTTYQLKFDDYSNVRLFVIESSSMVEVP
CCEEEEECCCCCEEECCCEEEEEECCEEEEECCCEEEEEECCCCCEEEEEEECCCCCHHH
KHFRNEYGQMLESAPYCERDIRTPELEDAIVEKGDFPLICKFGDKYQLTSLEWHPYDLVG
HHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHCCCCCCEEEEECCCEEEEEECCCCCEEEC
WDGCVYPWAFNIQEYAPKVGKIHLPPSDHIVFSAHNFVICNFVPRPYDFHPESIPAPYYH
CCCEECCEEECHHHHCCCCCEEECCCCCCEEEEECCEEEEEECCCCCCCCCCCCCCCCCC
SNIDSDEVLYYVDGDFMSRTGIEAGFMTLHQKGVPHGPQPGRTEASIGKTETYEYAVMVD
CCCCCCCEEEEECCCHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEE
TFAPLKLTEHVKHCMSNDYNRSWIES
CCCCHHHHHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure 
PSYVKQGIVPAKRHITFKKDNGELYREELFSTHGFSNIYSNKYHHNMPTKALAVSPYSL
CCHHHHCCCCCHHEEEEECCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEECCCCC
SHGEDWQDSLVQNYKLDTKQADCGGNFFSSRNKIFFNNDVAMYTAKVSEECDEFYRNAYA
CCCCCHHHHHHHHCCCCCCCCCCCCCHHCCCCEEEECCCEEEEEEHHHHHHHHHHHHCCC
DEVVFVHEGQGTLYSEYGALAVKKWDYLVIPRGTTYQLKFDDYSNVRLFVIESSSMVEVP
CCEEEEECCCCCEEECCCEEEEEECCEEEEECCCEEEEEECCCCCEEEEEEECCCCCHHH
KHFRNEYGQMLESAPYCERDIRTPELEDAIVEKGDFPLICKFGDKYQLTSLEWHPYDLVG
HHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHCCCCCCEEEEECCCEEEEEECCCCCEEEC
WDGCVYPWAFNIQEYAPKVGKIHLPPSDHIVFSAHNFVICNFVPRPYDFHPESIPAPYYH
CCCEECCEEECHHHHCCCCCEEECCCCCCEEEEECCEEEEEECCCCCCCCCCCCCCCCCC
SNIDSDEVLYYVDGDFMSRTGIEAGFMTLHQKGVPHGPQPGRTEASIGKTETYEYAVMVD
CCCCCCCEEEEECCCHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEE
TFAPLKLTEHVKHCMSNDYNRSWIES
CCCCHHHHHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: homogentisate; O2

Specific reaction: homogentisate + O2 = 4-maleylacetoacetate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]