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The map label for this gene is wcaJ [H]

Identifier: 157961241

GI number: 157961241

Start: 1716642

End: 1717238

Strand: Direct

Name: wcaJ [H]

Synonym: Spea_1414

Alternate gene names: 157961241

Gene position: 1716642-1717238 (Clockwise)

Preceding gene: 157961240

Following gene: 157961242

Centisome position: 33.17

GC content: 34.34

Gene sequence:

>597_bases
GTGATTAAACGGACTTTTGATTTTCTTCTATCTGGTGTCGGGTTATTATTTTTATCGCCTTTATTTTTAATCGTTGCTAT
ATGGATTAAAGTTGACTCTCCTGGGCCTATTTTTTTTAGACAGAGTAGGGTTGGTTTATTCGGAAGGGATTTTCTAATTC
ATAAGTTCAGAACAATGAGGGTTGATTCTGAATCATCTGGGCGTTTAACAGTTGGTAAAGATAGTCGAATCACACGTTCA
GGCCATTTTCTCAGGAAATCTAAAATTGACGAGTTACCACAATTGATAGATGTTCTGCTAGGCACAATGAGTTTGGTTGG
GCCTAGGCCTGAAGTTAGGGAGTTTATTGATTGTTATCCAGATGATGTTAAAGAAAAAGTTCTCTCTGTTAGGCCTGGTA
TTACAGATAGAGCTTCTATAGAGATGGTCGATGAAAATGAGATTTTGTCAAAGTATGAAGATCCCAGACAAGCTTATATA
GAGCATATATTACCAATTAAACAGAATTATTATATTGAATATGTAAAAAATAATACGTTACTTCTGGATGTTACTCTTAT
TTTTCAAACTTTGTTGAAAATTATTGGGAAGAGGTAA

Upstream 100 bases:

>100_bases
CAAGATATTTATGAGAGAGAAGTATCATTACCTTTGTATACAAAGATGGTAGAGTCTGATTTGAAAAGAGTTGTTGATGC
AGTTAAGAAGGTATTATTAG

Downstream 100 bases:

>100_bases
TTGTATTTGTAGCTAAATATGGTCTTTTGTGAAGACGTCGTGTTTTTCGGCGTTTTCACCGTTGGCTGTCATTTATCGCT
TTTAAACGTATCTAAAATTT

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MIKRTFDFLLSGVGLLFLSPLFLIVAIWIKVDSPGPIFFRQSRVGLFGRDFLIHKFRTMRVDSESSGRLTVGKDSRITRS
GHFLRKSKIDELPQLIDVLLGTMSLVGPRPEVREFIDCYPDDVKEKVLSVRPGITDRASIEMVDENEILSKYEDPRQAYI
EHILPIKQNYYIEYVKNNTLLLDVTLIFQTLLKIIGKR

Sequences:

>Translated_198_residues
MIKRTFDFLLSGVGLLFLSPLFLIVAIWIKVDSPGPIFFRQSRVGLFGRDFLIHKFRTMRVDSESSGRLTVGKDSRITRS
GHFLRKSKIDELPQLIDVLLGTMSLVGPRPEVREFIDCYPDDVKEKVLSVRPGITDRASIEMVDENEILSKYEDPRQAYI
EHILPIKQNYYIEYVKNNTLLLDVTLIFQTLLKIIGKR
>Mature_198_residues
MIKRTFDFLLSGVGLLFLSPLFLIVAIWIKVDSPGPIFFRQSRVGLFGRDFLIHKFRTMRVDSESSGRLTVGKDSRITRS
GHFLRKSKIDELPQLIDVLLGTMSLVGPRPEVREFIDCYPDDVKEKVLSVRPGITDRASIEMVDENEILSKYEDPRQAYI
EHILPIKQNYYIEYVKNNTLLLDVTLIFQTLLKIIGKR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=197, Percent_Identity=34.010152284264, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 22860; Mature: 22860

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRTFDFLLSGVGLLFLSPLFLIVAIWIKVDSPGPIFFRQSRVGLFGRDFLIHKFRTMR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCHHHHHHHHHHHHEE
VDSESSGRLTVGKDSRITRSGHFLRKSKIDELPQLIDVLLGTMSLVGPRPEVREFIDCYP
ECCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
DDVKEKVLSVRPGITDRASIEMVDENEILSKYEDPRQAYIEHILPIKQNYYIEYVKNNTL
HHHHHHHHHHCCCCCCCCEEEEECCHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCEE
LLDVTLIFQTLLKIIGKR
EEEHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKRTFDFLLSGVGLLFLSPLFLIVAIWIKVDSPGPIFFRQSRVGLFGRDFLIHKFRTMR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCHHHHHHHHHHHHEE
VDSESSGRLTVGKDSRITRSGHFLRKSKIDELPQLIDVLLGTMSLVGPRPEVREFIDCYP
ECCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
DDVKEKVLSVRPGITDRASIEMVDENEILSKYEDPRQAYIEHILPIKQNYYIEYVKNNTL
HHHHHHHHHHCCCCCCCCEEEEECCHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCEE
LLDVTLIFQTLLKIIGKR
EEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]