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The map label for this gene is cheB1 [H]

Identifier: 157961210

GI number: 157961210

Start: 1679983

End: 1681164

Strand: Direct

Name: cheB1 [H]

Synonym: Spea_1382

Alternate gene names: 157961210

Gene position: 1679983-1681164 (Clockwise)

Preceding gene: 157961209

Following gene: 157961211

Centisome position: 32.47

GC content: 47.88

Gene sequence:

>1182_bases
ATGGGCATTAAAGTTTTAGTCGTTGATGATTCGAGTTTTTTTAGACGACGTGTTAGTGAGATAGTGAATCAAGACCCTGA
GCTTGAGGTGGTGGGGACTGCCTGTAATGGTGCAGAGGCGGTGAAGATGGCTGCAGAGCTGAACCCGCAAGTGATCACCA
TGGATATCGAAATGCCTGTCATGGATGGGATCACTGCGGTTAAAGAGATCATGGCGAGTAAGCCAATTCCAATCTTGATG
TTTTCATCATTGACGCACGATGGTGCTAAGGCAACGTTAGAAGCGTTGGATGCCGGCGCTTTAGATTTCTTACCAAAACG
CTTTGAAGATATCGCAAGTAACAAAGATGACGCTATAGCGCTGTTACAGCAACGTATTCGAGCATTAGGCCGCCGTCGCT
TGTTTAGACCCATAACTCGAACATTGTCGAGTAACGATAGAGCGCTTTCAGGCAATGAAAGAGCTCAATCTGTCACAGAT
AGATCTCGCCCAATCTCAAGTTTTTCTCGAACGAGTGTTAATGCACCAGCAACTAGCGGTCACTCGTTAACTGCTAGCGA
TAAGCCCGTGAGTCGTTCTAGTGTTGTGACCCGTAGTAGTGGAAAACAATACAAGACGTTATTAATCGGTACCTCTACAG
GTGGGCCTGTTGCTTTACAAAAGGTGTTAACTCAATTCCCTGCCAATTATCCACATCCTATTTTGCTTATCCAACATATG
CCTGCTGCCTTTACGCCAACATTTGCGGCTAGGCTTAATGGCTTGTGTAAGATAGAAGTTAAAGAGGCGGTGAGCGGAGA
CCAGTTAAGGCCGGGTTGTGCATATCTTGCCCCTGGAGGTATGCAGATGATGCTAGAGCGAGGCGGAGCCTATGGCAGAG
TTAAGATCATTGCTGGTAAGGCGGATATGAACTACAAGCCTTGTGTCGATATTACTTTTGCCTCAGCTTCGAAGGTGCTT
GGTGGTGATGTGTTGGCCGTAGTGCTCACGGGCATGGGAGCCGATGGCCGTGAAGGGGCCAGAATGCTCAAGAGTATCGG
TGCGACCATTTGGGCGCAAGATGAGGCCAGCTGTGTGGTTTATGGCATGCCTCAAGCCGTCGCGGCTGCGGGTATTGCAA
CCAAATCAATTGCGTTAGAGCATATGGCTGAATCGATTTTAAAAGAGACCGTTAATGGCTAA

Upstream 100 bases:

>100_bases
ACAGGAGCTAACACTGGTTAGCATAGGGAATAACGCAGCTTGTTATTCCCTTTTTTACATACAGCATTGAGCAGGCCTGT
GGTGCAGTAAAGGAATTTGA

Downstream 100 bases:

>100_bases
GGCAGCTTTGAAGCAGAGTGTCATTCAGCAACGGGGAGAAATCAATACGCTGGCGCTGCTGTTAATGTTTTTGCTGAGTA
TTGCGTCATTTACCTTCGGC

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MGIKVLVVDDSSFFRRRVSEIVNQDPELEVVGTACNGAEAVKMAAELNPQVITMDIEMPVMDGITAVKEIMASKPIPILM
FSSLTHDGAKATLEALDAGALDFLPKRFEDIASNKDDAIALLQQRIRALGRRRLFRPITRTLSSNDRALSGNERAQSVTD
RSRPISSFSRTSVNAPATSGHSLTASDKPVSRSSVVTRSSGKQYKTLLIGTSTGGPVALQKVLTQFPANYPHPILLIQHM
PAAFTPTFAARLNGLCKIEVKEAVSGDQLRPGCAYLAPGGMQMMLERGGAYGRVKIIAGKADMNYKPCVDITFASASKVL
GGDVLAVVLTGMGADGREGARMLKSIGATIWAQDEASCVVYGMPQAVAAAGIATKSIALEHMAESILKETVNG

Sequences:

>Translated_393_residues
MGIKVLVVDDSSFFRRRVSEIVNQDPELEVVGTACNGAEAVKMAAELNPQVITMDIEMPVMDGITAVKEIMASKPIPILM
FSSLTHDGAKATLEALDAGALDFLPKRFEDIASNKDDAIALLQQRIRALGRRRLFRPITRTLSSNDRALSGNERAQSVTD
RSRPISSFSRTSVNAPATSGHSLTASDKPVSRSSVVTRSSGKQYKTLLIGTSTGGPVALQKVLTQFPANYPHPILLIQHM
PAAFTPTFAARLNGLCKIEVKEAVSGDQLRPGCAYLAPGGMQMMLERGGAYGRVKIIAGKADMNYKPCVDITFASASKVL
GGDVLAVVLTGMGADGREGARMLKSIGATIWAQDEASCVVYGMPQAVAAAGIATKSIALEHMAESILKETVNG
>Mature_392_residues
GIKVLVVDDSSFFRRRVSEIVNQDPELEVVGTACNGAEAVKMAAELNPQVITMDIEMPVMDGITAVKEIMASKPIPILMF
SSLTHDGAKATLEALDAGALDFLPKRFEDIASNKDDAIALLQQRIRALGRRRLFRPITRTLSSNDRALSGNERAQSVTDR
SRPISSFSRTSVNAPATSGHSLTASDKPVSRSSVVTRSSGKQYKTLLIGTSTGGPVALQKVLTQFPANYPHPILLIQHMP
AAFTPTFAARLNGLCKIEVKEAVSGDQLRPGCAYLAPGGMQMMLERGGAYGRVKIIAGKADMNYKPCVDITFASASKVLG
GDVLAVVLTGMGADGREGARMLKSIGATIWAQDEASCVVYGMPQAVAAAGIATKSIALEHMAESILKETVNG

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR [H]

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=394, Percent_Identity=36.0406091370558, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1790299, Length=190, Percent_Identity=31.0526315789474, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788550, Length=145, Percent_Identity=28.9655172413793, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789 [H]

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg [H]

EC number: =3.1.1.61 [H]

Molecular weight: Translated: 41791; Mature: 41660

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50122 CHEB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIKVLVVDDSSFFRRRVSEIVNQDPELEVVGTACNGAEAVKMAAELNPQVITMDIEMPV
CCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCEEEEEEECCCH
MDGITAVKEIMASKPIPILMFSSLTHDGAKATLEALDAGALDFLPKRFEDIASNKDDAIA
HHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHH
LLQQRIRALGRRRLFRPITRTLSSNDRALSGNERAQSVTDRSRPISSFSRTSVNAPATSG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCC
HSLTASDKPVSRSSVVTRSSGKQYKTLLIGTSTGGPVALQKVLTQFPANYPHPILLIQHM
CEECCCCCCCCHHHEEECCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECC
PAAFTPTFAARLNGLCKIEVKEAVSGDQLRPGCAYLAPGGMQMMLERGGAYGRVKIIAGK
CCCCCCHHHHHHCCEEEEEEHHCCCCCCCCCCEEEECCCHHHHHHHCCCCCEEEEEEEEC
ADMNYKPCVDITFASASKVLGGDVLAVVLTGMGADGREGARMLKSIGATIWAQDEASCVV
CCCCCCCCEEEEECCHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCCCCEEE
YGMPQAVAAAGIATKSIALEHMAESILKETVNG
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GIKVLVVDDSSFFRRRVSEIVNQDPELEVVGTACNGAEAVKMAAELNPQVITMDIEMPV
CEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCEEEEEEECCCH
MDGITAVKEIMASKPIPILMFSSLTHDGAKATLEALDAGALDFLPKRFEDIASNKDDAIA
HHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHH
LLQQRIRALGRRRLFRPITRTLSSNDRALSGNERAQSVTDRSRPISSFSRTSVNAPATSG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCC
HSLTASDKPVSRSSVVTRSSGKQYKTLLIGTSTGGPVALQKVLTQFPANYPHPILLIQHM
CEECCCCCCCCHHHEEECCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECC
PAAFTPTFAARLNGLCKIEVKEAVSGDQLRPGCAYLAPGGMQMMLERGGAYGRVKIIAGK
CCCCCCHHHHHHCCEEEEEEHHCCCCCCCCCCEEEECCCHHHHHHHCCCCCEEEEEEEEC
ADMNYKPCVDITFASASKVLGGDVLAVVLTGMGADGREGARMLKSIGATIWAQDEASCVV
CCCCCCCCEEEEECCHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCCCCEEE
YGMPQAVAAAGIATKSIALEHMAESILKETVNG
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]