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The map label for this gene is gltT [H]

Identifier: 157961034

GI number: 157961034

Start: 1467001

End: 1468251

Strand: Reverse

Name: gltT [H]

Synonym: Spea_1206

Alternate gene names: 157961034

Gene position: 1468251-1467001 (Counterclockwise)

Preceding gene: 157961042

Following gene: 157961033

Centisome position: 28.37

GC content: 47.32

Gene sequence:

>1251_bases
ATGTGGCAAGCGATTAAAAACATCCCATTTTGGCAGAAGGTACTAGCAGCCTTTATATTGGGCGCCGGTGTTGGTGTCAT
TTTCGGTGAGTCGGCAACTGTATTAAAGCCACTTGGCGATCTATTTATCAGCGCAATTAAGATGTTAGTTGCGCCACTCA
TTTTCTGCGCCATCGTTGTCAGCATCACCTCTTTAGGCTCTGAAGTCAGTTTAAAAAGACTGAGCTTTAAAACCTTAGGC
ATGTTCATGCTTACTGGCACTATAGCCTCTCTCATTGGATTAACCATTGGTTCTGTGATTGATATGGGCGGTTCGATGGA
GCTGGTTGCTACTGAGGTTCGTGAACGCAATGTTCCGGGTTTTGCTCAAGTTTTATTGGATATGATCCCGGTTAACCCAT
TTGCTTCGCTTGCCGAAGGTAAGGTGTTACAGATCATCGTCTTCGCTGCACTTATCGGTATTGCGATTAATAAAGTCGGC
GAAAAAGCAGCCCCGCTAAAGCGTACGATTGAAGCAGGCGCTGAAGTGATGTTCCAACTTACTCGCATGGTACTGCAACT
CACCCCAATTGGTGTGTTTGGTTTAATGGCTTGGGTAGTTGGCGAATACGGCTTATCGATGTTACTGCCTTTAGGTAAGT
TTATTGGTGCTATTTATCTTGCTGCACTTATCCATATTATTTTCGTCTACGGTGGTTTGGTTAAGTTTGTCGCCAAGCTG
AGTCCAGTACAATTTTTCCGTAAAGCGATACCAGCACAAATAGTGGCTTTCACCACGGCTTCAAGTTTTGGTACTTTGCC
AGCAAGCACACGTTGCACCGAGTCTATGGGTGTCTCTAAGCGTTATGGCTCTTTCGTCCTGCCATTAGGTGCCACCATGA
ACATGGATGGCTGTGGCGGTATTTACCCAGCGATTGCGGCTATTTTCATTGCGCAAATCTACGGTATTCCACTTGATATG
ACTGACTATATGTTGATTGCCGTAACCGCGACCGTTGCCTCCGTTGGTACCGCTGGCGTACCTGGCAGCGCCATGGTTAT
GCTCACAGTCACCTTGGGCGTTGTTGGTTTGCCACTTGAAGGCATCGCCTTTATCGCTGCAGTCGATCGCATTATCGATA
TGATCCGCACCGCCACCAATGTCACCGGCGATATGATGACCGCTGTGGTGATTGGTAAGTCTGAAGGCCAGCTGGATGAA
ACGCAGTTTTACACTCCAGAGAATGGCTCGCTAGAGCAGGCCAAGAGCTAA

Upstream 100 bases:

>100_bases
AATGTAAATTTAAACAGTTATTGCCTGAAACTAATACTGGAGTGAACAAAGATTCACCCGTAGAGTAACTTGATAAAAAT
TAAATAATTGGAATTCGATA

Downstream 100 bases:

>100_bases
ACTCAGACATTTACTGAATAAAAAGGCCGCAATTGCGGCCTTTTTAATTTATACTTTTCAATATTATACTGGGTAACTAG
GTTATTTTAGGAGCGGCTGG

Product: sodium:dicarboxylate symporter

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MWQAIKNIPFWQKVLAAFILGAGVGVIFGESATVLKPLGDLFISAIKMLVAPLIFCAIVVSITSLGSEVSLKRLSFKTLG
MFMLTGTIASLIGLTIGSVIDMGGSMELVATEVRERNVPGFAQVLLDMIPVNPFASLAEGKVLQIIVFAALIGIAINKVG
EKAAPLKRTIEAGAEVMFQLTRMVLQLTPIGVFGLMAWVVGEYGLSMLLPLGKFIGAIYLAALIHIIFVYGGLVKFVAKL
SPVQFFRKAIPAQIVAFTTASSFGTLPASTRCTESMGVSKRYGSFVLPLGATMNMDGCGGIYPAIAAIFIAQIYGIPLDM
TDYMLIAVTATVASVGTAGVPGSAMVMLTVTLGVVGLPLEGIAFIAAVDRIIDMIRTATNVTGDMMTAVVIGKSEGQLDE
TQFYTPENGSLEQAKS

Sequences:

>Translated_416_residues
MWQAIKNIPFWQKVLAAFILGAGVGVIFGESATVLKPLGDLFISAIKMLVAPLIFCAIVVSITSLGSEVSLKRLSFKTLG
MFMLTGTIASLIGLTIGSVIDMGGSMELVATEVRERNVPGFAQVLLDMIPVNPFASLAEGKVLQIIVFAALIGIAINKVG
EKAAPLKRTIEAGAEVMFQLTRMVLQLTPIGVFGLMAWVVGEYGLSMLLPLGKFIGAIYLAALIHIIFVYGGLVKFVAKL
SPVQFFRKAIPAQIVAFTTASSFGTLPASTRCTESMGVSKRYGSFVLPLGATMNMDGCGGIYPAIAAIFIAQIYGIPLDM
TDYMLIAVTATVASVGTAGVPGSAMVMLTVTLGVVGLPLEGIAFIAAVDRIIDMIRTATNVTGDMMTAVVIGKSEGQLDE
TQFYTPENGSLEQAKS
>Mature_416_residues
MWQAIKNIPFWQKVLAAFILGAGVGVIFGESATVLKPLGDLFISAIKMLVAPLIFCAIVVSITSLGSEVSLKRLSFKTLG
MFMLTGTIASLIGLTIGSVIDMGGSMELVATEVRERNVPGFAQVLLDMIPVNPFASLAEGKVLQIIVFAALIGIAINKVG
EKAAPLKRTIEAGAEVMFQLTRMVLQLTPIGVFGLMAWVVGEYGLSMLLPLGKFIGAIYLAALIHIIFVYGGLVKFVAKL
SPVQFFRKAIPAQIVAFTTASSFGTLPASTRCTESMGVSKRYGSFVLPLGATMNMDGCGGIYPAIAAIFIAQIYGIPLDM
TDYMLIAVTATVASVGTAGVPGSAMVMLTVTLGVVGLPLEGIAFIAAVDRIIDMIRTATNVTGDMMTAVVIGKSEGQLDE
TQFYTPENGSLEQAKS

Specific function: This carrier protein is part of the Na(+)-dependent, binding-protein-independent glutamate-aspartate transport system [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI4827012, Length=301, Percent_Identity=35.2159468438538, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI194239697, Length=289, Percent_Identity=34.2560553633218, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI40254478, Length=282, Percent_Identity=35.4609929078014, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI66773030, Length=271, Percent_Identity=34.3173431734317, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI21314632, Length=436, Percent_Identity=28.6697247706422, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI169790839, Length=252, Percent_Identity=35.3174603174603, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI223468566, Length=313, Percent_Identity=33.2268370607029, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI223468564, Length=279, Percent_Identity=34.4086021505376, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI5032093, Length=311, Percent_Identity=33.4405144694534, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI301601644, Length=183, Percent_Identity=36.0655737704918, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI262359914, Length=180, Percent_Identity=32.7777777777778, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI1790514, Length=422, Percent_Identity=36.4928909952607, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1789947, Length=416, Percent_Identity=31.7307692307692, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1788024, Length=422, Percent_Identity=30.3317535545024, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1789473, Length=378, Percent_Identity=24.0740740740741, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71996953, Length=428, Percent_Identity=30.8411214953271, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI71983106, Length=415, Percent_Identity=31.3253012048193, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI71983099, Length=415, Percent_Identity=31.3253012048193, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17537407, Length=384, Percent_Identity=30.9895833333333, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI193206505, Length=429, Percent_Identity=27.2727272727273, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI193206654, Length=420, Percent_Identity=28.3333333333333, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17541374, Length=392, Percent_Identity=27.2959183673469, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24583025, Length=403, Percent_Identity=26.0545905707196, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17137668, Length=403, Percent_Identity=26.0545905707196, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24583023, Length=403, Percent_Identity=26.0545905707196, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI281360483, Length=429, Percent_Identity=27.039627039627, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI17137666, Length=429, Percent_Identity=27.039627039627, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI281360481, Length=429, Percent_Identity=27.039627039627, Blast_Score=99, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 43838; Mature: 43838

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEECCCCCCCCCEEECCCCCCHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEECCCCCCCCCEEECCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]