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The map label for this gene is phoA [H]

Identifier: 157960973

GI number: 157960973

Start: 1382441

End: 1383940

Strand: Reverse

Name: phoA [H]

Synonym: Spea_1145

Alternate gene names: 157960973

Gene position: 1383940-1382441 (Counterclockwise)

Preceding gene: 157960974

Following gene: 157960972

Centisome position: 26.74

GC content: 48.87

Gene sequence:

>1500_bases
GTGAATAATATCAAAAAAATAGCTGTGATTATCTCATCCGCTCTAGCAACTTTCGCGGCCAATGCTGCTGTATTGCCCAG
CAGCCAAACTGACAGTCAATGGTACCAAGACAGCGCCAAGCTCGTCAGTGAAAAAACCAAACAAACGAGTGCTACAACAG
CTAAAAACGTCATTCTATTTGTCGGTGACGGTATGGGAGTATCGACACTCACTGCGGCACGTATTTTTGAAGGACAACAA
CAAGCTAATAACCAAGGCGGAGAGGAAAATTTCCTAAGCTTTGAGCTGTTCCCAAAAACGGCGCTAGTTAAAACCTACAA
CACCAATCAGCAAACACCCGACTCAGCAGGCACCATGACGGCTATCGCTACTGGCGTAAAATCTAAGGCGGGTGTGTTAT
CGGTATCAGATACCAGCCTTAGAGCCAATTGCTTATCTTCAAAGGGTAATGAACTGGTGACCTTAGTCGATTTAGCCAAT
GCCAAAGGACTCTCTACTGGCATTGTTAGTACGGCGAGGATCACCCATGCAACGCCAGCAGCAACTTATGCCACCTCTCC
TGAGCGTAATTGGGAGGCCGACTCAAACCTTCCCACAGAAGCCGTTGCCAACGAGTGCAAGGATATTGCTTATCAACTGG
TGATGCGTGATGAAGTTGACGCGCTGACGGTTGCCTTAGGTGGCGGACGTAGAAACTTCATCCCCAGTGATATGACCGAC
GGCGAAGATAAGGCTGGTCGCCGTGAAGATGGTTTAGATCTAACAAAGTCTTGGACTGACAACCTGAGTAACTCGGCTTA
CGTTTGGGACAAAGCGGGATTCGACGCTGTCGATGTCGCCTCGACCGATCATCTGCTTGGCTTATTTAATTCGTCACATA
TGCAGTATGAAGCCGACCGTTTAGATGACACAGCAGGAGAGCCCTCGTTAACCGAGATGACCACTAAATCGATAGAACTG
CTCAATAAAAATGACCAAGGTTACTTATTGATAGTGGAATCGGGTCGAATCGATCACGCTCACCATGCCGGTAATGCCTA
TCGCGCCATGATGGATACTGTTGAGCTTTCGAATGCCGTTCGCGCCGCGGTCGAAAACACCAATCCTGAAGAAACCTTAA
TCATGGTCACCGCCGACCATAGTCACGTATTTACCATTGCGGGTTATCCAAAGCGTGGCAACCCAATATTAGGCCTAGTG
CATGGTATCGATGGCAGTGTCACTATCGCCAAAGACGGTAAACCCTATACCACAGTTGGCTACACCAATGGCCCTGGAGC
CGTGGTCGGTCTTCGCGATGACCTAAGCTCTGTCGATACTCAAGATAAAGAGTTTATGCAGCAAGCGCTCGTGCCAATGG
GCAGTGAAACTCACGCTGGCGAAGATATTACTCTGCACGCTACAGGAGCGGGGTCAGATCTCGTTCAGGGCGTAATGGAG
CAAAACGTTATCTTCCACATCATCAACCAGGCCCAATCCCTCGGTGGCACCAAATACTAA

Upstream 100 bases:

>100_bases
ACCGACATAAATCCGACTCAATAGCGTCATACTTTTGCCCGACAATCACCAAGCCACAAAGGAATCTACCTATAAACCTT
ACAAATTTTCGGTGATAATA

Downstream 100 bases:

>100_bases
CCAATGAGTACTCATATCAGCAGTACGAATTAACTACTGACACTCACTGACGAATCCGTCAGCACGAGTACCCATTTATG
GAGTTAAAAATGAACAAGAA

Product: alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MNNIKKIAVIISSALATFAANAAVLPSSQTDSQWYQDSAKLVSEKTKQTSATTAKNVILFVGDGMGVSTLTAARIFEGQQ
QANNQGGEENFLSFELFPKTALVKTYNTNQQTPDSAGTMTAIATGVKSKAGVLSVSDTSLRANCLSSKGNELVTLVDLAN
AKGLSTGIVSTARITHATPAATYATSPERNWEADSNLPTEAVANECKDIAYQLVMRDEVDALTVALGGGRRNFIPSDMTD
GEDKAGRREDGLDLTKSWTDNLSNSAYVWDKAGFDAVDVASTDHLLGLFNSSHMQYEADRLDDTAGEPSLTEMTTKSIEL
LNKNDQGYLLIVESGRIDHAHHAGNAYRAMMDTVELSNAVRAAVENTNPEETLIMVTADHSHVFTIAGYPKRGNPILGLV
HGIDGSVTIAKDGKPYTTVGYTNGPGAVVGLRDDLSSVDTQDKEFMQQALVPMGSETHAGEDITLHATGAGSDLVQGVME
QNVIFHIINQAQSLGGTKY

Sequences:

>Translated_499_residues
MNNIKKIAVIISSALATFAANAAVLPSSQTDSQWYQDSAKLVSEKTKQTSATTAKNVILFVGDGMGVSTLTAARIFEGQQ
QANNQGGEENFLSFELFPKTALVKTYNTNQQTPDSAGTMTAIATGVKSKAGVLSVSDTSLRANCLSSKGNELVTLVDLAN
AKGLSTGIVSTARITHATPAATYATSPERNWEADSNLPTEAVANECKDIAYQLVMRDEVDALTVALGGGRRNFIPSDMTD
GEDKAGRREDGLDLTKSWTDNLSNSAYVWDKAGFDAVDVASTDHLLGLFNSSHMQYEADRLDDTAGEPSLTEMTTKSIEL
LNKNDQGYLLIVESGRIDHAHHAGNAYRAMMDTVELSNAVRAAVENTNPEETLIMVTADHSHVFTIAGYPKRGNPILGLV
HGIDGSVTIAKDGKPYTTVGYTNGPGAVVGLRDDLSSVDTQDKEFMQQALVPMGSETHAGEDITLHATGAGSDLVQGVME
QNVIFHIINQAQSLGGTKY
>Mature_499_residues
MNNIKKIAVIISSALATFAANAAVLPSSQTDSQWYQDSAKLVSEKTKQTSATTAKNVILFVGDGMGVSTLTAARIFEGQQ
QANNQGGEENFLSFELFPKTALVKTYNTNQQTPDSAGTMTAIATGVKSKAGVLSVSDTSLRANCLSSKGNELVTLVDLAN
AKGLSTGIVSTARITHATPAATYATSPERNWEADSNLPTEAVANECKDIAYQLVMRDEVDALTVALGGGRRNFIPSDMTD
GEDKAGRREDGLDLTKSWTDNLSNSAYVWDKAGFDAVDVASTDHLLGLFNSSHMQYEADRLDDTAGEPSLTEMTTKSIEL
LNKNDQGYLLIVESGRIDHAHHAGNAYRAMMDTVELSNAVRAAVENTNPEETLIMVTADHSHVFTIAGYPKRGNPILGLV
HGIDGSVTIAKDGKPYTTVGYTNGPGAVVGLRDDLSSVDTQDKEFMQQALVPMGSETHAGEDITLHATGAGSDLVQGVME
QNVIFHIINQAQSLGGTKY

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=504, Percent_Identity=42.4603174603175, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI294660770, Length=449, Percent_Identity=44.543429844098, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI157266296, Length=481, Percent_Identity=40.1247401247401, Blast_Score=360, Evalue=2e-99,
Organism=Homo sapiens, GI94721246, Length=470, Percent_Identity=41.063829787234, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI157266292, Length=481, Percent_Identity=39.9168399168399, Blast_Score=351, Evalue=1e-96,
Organism=Homo sapiens, GI294660772, Length=403, Percent_Identity=43.1761786600496, Blast_Score=323, Evalue=2e-88,
Organism=Escherichia coli, GI48994877, Length=394, Percent_Identity=31.9796954314721, Blast_Score=125, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6320689, Length=350, Percent_Identity=29.1428571428571, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355149, Length=489, Percent_Identity=40.2862985685072, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI21355981, Length=482, Percent_Identity=39.6265560165975, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI19921912, Length=492, Percent_Identity=40.0406504065041, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI21355151, Length=480, Percent_Identity=40, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24585213, Length=498, Percent_Identity=38.5542168674699, Blast_Score=305, Evalue=6e-83,
Organism=Drosophila melanogaster, GI24651554, Length=489, Percent_Identity=38.241308793456, Blast_Score=300, Evalue=2e-81,
Organism=Drosophila melanogaster, GI85725200, Length=458, Percent_Identity=38.4279475982533, Blast_Score=298, Evalue=4e-81,
Organism=Drosophila melanogaster, GI85815833, Length=458, Percent_Identity=38.4279475982533, Blast_Score=298, Evalue=4e-81,
Organism=Drosophila melanogaster, GI21358067, Length=476, Percent_Identity=38.655462184874, Blast_Score=298, Evalue=7e-81,
Organism=Drosophila melanogaster, GI24657842, Length=499, Percent_Identity=36.2725450901804, Blast_Score=289, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24657835, Length=498, Percent_Identity=35.9437751004016, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24657827, Length=482, Percent_Identity=37.344398340249, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24651556, Length=463, Percent_Identity=38.0129589632829, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI18859923, Length=478, Percent_Identity=34.1004184100418, Blast_Score=237, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24645593, Length=313, Percent_Identity=27.4760383386581, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 53160; Mature: 53160

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIKKIAVIISSALATFAANAAVLPSSQTDSQWYQDSAKLVSEKTKQTSATTAKNVILF
CCCHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
VGDGMGVSTLTAARIFEGQQQANNQGGEENFLSFELFPKTALVKTYNTNQQTPDSAGTMT
EECCCCHHHHHHHHHHCCHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCHH
AIATGVKSKAGVLSVSDTSLRANCLSSKGNELVTLVDLANAKGLSTGIVSTARITHATPA
HHHHCCCCCCCEEEECCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCC
ATYATSPERNWEADSNLPTEAVANECKDIAYQLVMRDEVDALTVALGGGRRNFIPSDMTD
CEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
GEDKAGRREDGLDLTKSWTDNLSNSAYVWDKAGFDAVDVASTDHLLGLFNSSHMQYEADR
CCCCCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCEEECCCCCHHEEEECCCCCEEEHHH
LDDTAGEPSLTEMTTKSIELLNKNDQGYLLIVESGRIDHAHHAGNAYRAMMDTVELSNAV
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHH
RAAVENTNPEETLIMVTADHSHVFTIAGYPKRGNPILGLVHGIDGSVTIAKDGKPYTTVG
HHHHCCCCCCCEEEEEEECCCEEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCEEEEE
YTNGPGAVVGLRDDLSSVDTQDKEFMQQALVPMGSETHAGEDITLHATGAGSDLVQGVME
EECCCCEEEEEHHCHHHCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHH
QNVIFHIINQAQSLGGTKY
CCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNNIKKIAVIISSALATFAANAAVLPSSQTDSQWYQDSAKLVSEKTKQTSATTAKNVILF
CCCHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
VGDGMGVSTLTAARIFEGQQQANNQGGEENFLSFELFPKTALVKTYNTNQQTPDSAGTMT
EECCCCHHHHHHHHHHCCHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCHH
AIATGVKSKAGVLSVSDTSLRANCLSSKGNELVTLVDLANAKGLSTGIVSTARITHATPA
HHHHCCCCCCCEEEECCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCC
ATYATSPERNWEADSNLPTEAVANECKDIAYQLVMRDEVDALTVALGGGRRNFIPSDMTD
CEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
GEDKAGRREDGLDLTKSWTDNLSNSAYVWDKAGFDAVDVASTDHLLGLFNSSHMQYEADR
CCCCCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCEEECCCCCHHEEEECCCCCEEEHHH
LDDTAGEPSLTEMTTKSIELLNKNDQGYLLIVESGRIDHAHHAGNAYRAMMDTVELSNAV
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHH
RAAVENTNPEETLIMVTADHSHVFTIAGYPKRGNPILGLVHGIDGSVTIAKDGKPYTTVG
HHHHCCCCCCCEEEEEEECCCEEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCEEEEE
YTNGPGAVVGLRDDLSSVDTQDKEFMQQALVPMGSETHAGEDITLHATGAGSDLVQGVME
EECCCCEEEEEHHCHHHCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHH
QNVIFHIINQAQSLGGTKY
CCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]