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The map label for this gene is phoA [H]

Identifier: 157960972

GI number: 157960972

Start: 1380744

End: 1382351

Strand: Reverse

Name: phoA [H]

Synonym: Spea_1144

Alternate gene names: 157960972

Gene position: 1382351-1380744 (Counterclockwise)

Preceding gene: 157960973

Following gene: 157960971

Centisome position: 26.71

GC content: 51.24

Gene sequence:

>1608_bases
ATGAACAAGAAATTACTGGTACTATCAATTACAGCTATGTTGGGCCTAGCCGCTTGTGGCGATGATGGTGACAAAGGCGC
CGATGGTAACGATGGTCAAGATTGGACTGCGGTCAACCAATGGTTCGTTGACGGTCAATCCCGCGTCACGAAGGCACAAG
AGCTAAGCGTTAATAATGAAGCTGGCGCCGCAAAAAACATCATTCTATTTGTGGGTGATGGCATGGGCGTATCAACTGTA
ACTGCGGCGCGGATCCTAGAAGGACAATTAAAAGGGCAAACCGGTGAAGAAAACTCGCTGTCTTTTGAGACCTTACCTTA
TGTCGGCCTAGCCAAAACCTACAACGTCGACGGCCAAACACCGGACTCTGCGGGCACCATGACTGCCATGGTCACCGGCG
TTAAAACTGATGTGGGCGTGATTTCACAAGCTGAAGGGGTAACTCGCGGTAACTGCGCCTCGACCGATGGCCAAAACTTA
GTGACCTCGCTTGAACTGGCGGCAATGGCCGGCTTATCGACCGGCGTTGTCTCTACTGCACGCATTACTCACGCAACTCC
AGCGGCAGCTTATGCCCATGCCCCCGAGCGTAACTGGGAAAGTGATGCTGACTTACCCGATGAAGCCATCACTAATGGTT
GTAGTGACATCGCATCGCAGCTTCTAAGCTTTGATTATGGCGCGGGGCTCAATGTGGTCATGGGCGGAGGCCGTCGCGCA
TTTATCCCTAACACTGTGACTGACACAGAAGGTAAAGCCGGTAAGCGATTAGATAGTCGCGATCTCACCTCTGAGTGGCT
AACGAAATATACCAACAGTGCGTATGTCGCCAGTAGAGATGAGTTCTTAGCACTCGATCCTGCTACTACCGACCATGCTC
TTGGCTTATTTAATTCATCACATATGGAGTATGAACTCGACCGCACTACGGATGGGGTCACGGGCGAACCTTCATTGGCT
GAAATGACCGCCAAGTCTATCGATATATTGCAGAAAAACGATAAAGGTTTTGTGCTAATTATTGAAGCAGGACGCATCGA
CCATGCTCACCATGCTGGCAACGCCGCCCGCGCTTTACACGACACTATCGCACTGTCTGAAGCGGTGCGCGTCGCCATGG
AAAAAACCTCTGAAAAAGACACCCTGTTAATGGTTACCGCAGATCACAGCCATGTATTTACCATTGCAGGCTACCCCACT
CGCGGCAATCCAATTTTAGGCCTAGTGAAAGGTAACGGCGCTGACGGACAGCCTGCCGTGACTAATGCAACCGATGCCAA
TGGTTTACCTTACACCACTGTCGGTTATGCCAACGGTCTTGGTTACGCCAATCTTGAAACCGGTGGTGATGAGCGTTATG
GCTACGCAGCGGCGCCCGGGCGATTTGACCTCAATTATGTCGATACTCAAAGCGCGGGTTTTCACCAAGAAGCACTCGTG
CCCCTTGGCAGCGAAACCCATGCGGGTGAAGACGTAGGGATTTTTGCAAGCGGCCCCGGTGCTCACTTAGTGCAAGGCAC
GGTTGAACAAAATCATCTTTTCCATGTGATGAATCATGCTGGCAGCTTAGTCGAAAAAGCTGAGGCGGCTCAGTCAACTT
CGAATTAA

Upstream 100 bases:

>100_bases
CCAAATACTAACCAATGAGTACTCATATCAGCAGTACGAATTAACTACTGACACTCACTGACGAATCCGTCAGCACGAGT
ACCCATTTATGGAGTTAAAA

Downstream 100 bases:

>100_bases
CGACCTTAGCGCCATGAATTGCCACCGAGAAGTCACTCGGTGGCCTGACGCTATGCCAACTCTATCCCATTTCACTAGGG
CGTTATCCGAGGCTTTATGT

Product: alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MNKKLLVLSITAMLGLAACGDDGDKGADGNDGQDWTAVNQWFVDGQSRVTKAQELSVNNEAGAAKNIILFVGDGMGVSTV
TAARILEGQLKGQTGEENSLSFETLPYVGLAKTYNVDGQTPDSAGTMTAMVTGVKTDVGVISQAEGVTRGNCASTDGQNL
VTSLELAAMAGLSTGVVSTARITHATPAAAYAHAPERNWESDADLPDEAITNGCSDIASQLLSFDYGAGLNVVMGGGRRA
FIPNTVTDTEGKAGKRLDSRDLTSEWLTKYTNSAYVASRDEFLALDPATTDHALGLFNSSHMEYELDRTTDGVTGEPSLA
EMTAKSIDILQKNDKGFVLIIEAGRIDHAHHAGNAARALHDTIALSEAVRVAMEKTSEKDTLLMVTADHSHVFTIAGYPT
RGNPILGLVKGNGADGQPAVTNATDANGLPYTTVGYANGLGYANLETGGDERYGYAAAPGRFDLNYVDTQSAGFHQEALV
PLGSETHAGEDVGIFASGPGAHLVQGTVEQNHLFHVMNHAGSLVEKAEAAQSTSN

Sequences:

>Translated_535_residues
MNKKLLVLSITAMLGLAACGDDGDKGADGNDGQDWTAVNQWFVDGQSRVTKAQELSVNNEAGAAKNIILFVGDGMGVSTV
TAARILEGQLKGQTGEENSLSFETLPYVGLAKTYNVDGQTPDSAGTMTAMVTGVKTDVGVISQAEGVTRGNCASTDGQNL
VTSLELAAMAGLSTGVVSTARITHATPAAAYAHAPERNWESDADLPDEAITNGCSDIASQLLSFDYGAGLNVVMGGGRRA
FIPNTVTDTEGKAGKRLDSRDLTSEWLTKYTNSAYVASRDEFLALDPATTDHALGLFNSSHMEYELDRTTDGVTGEPSLA
EMTAKSIDILQKNDKGFVLIIEAGRIDHAHHAGNAARALHDTIALSEAVRVAMEKTSEKDTLLMVTADHSHVFTIAGYPT
RGNPILGLVKGNGADGQPAVTNATDANGLPYTTVGYANGLGYANLETGGDERYGYAAAPGRFDLNYVDTQSAGFHQEALV
PLGSETHAGEDVGIFASGPGAHLVQGTVEQNHLFHVMNHAGSLVEKAEAAQSTSN
>Mature_535_residues
MNKKLLVLSITAMLGLAACGDDGDKGADGNDGQDWTAVNQWFVDGQSRVTKAQELSVNNEAGAAKNIILFVGDGMGVSTV
TAARILEGQLKGQTGEENSLSFETLPYVGLAKTYNVDGQTPDSAGTMTAMVTGVKTDVGVISQAEGVTRGNCASTDGQNL
VTSLELAAMAGLSTGVVSTARITHATPAAAYAHAPERNWESDADLPDEAITNGCSDIASQLLSFDYGAGLNVVMGGGRRA
FIPNTVTDTEGKAGKRLDSRDLTSEWLTKYTNSAYVASRDEFLALDPATTDHALGLFNSSHMEYELDRTTDGVTGEPSLA
EMTAKSIDILQKNDKGFVLIIEAGRIDHAHHAGNAARALHDTIALSEAVRVAMEKTSEKDTLLMVTADHSHVFTIAGYPT
RGNPILGLVKGNGADGQPAVTNATDANGLPYTTVGYANGLGYANLETGGDERYGYAAAPGRFDLNYVDTQSAGFHQEALV
PLGSETHAGEDVGIFASGPGAHLVQGTVEQNHLFHVMNHAGSLVEKAEAAQSTSN

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=491, Percent_Identity=43.9918533604888, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI294660770, Length=463, Percent_Identity=44.4924406047516, Blast_Score=384, Evalue=1e-106,
Organism=Homo sapiens, GI157266296, Length=468, Percent_Identity=44.8717948717949, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI94721246, Length=468, Percent_Identity=44.4444444444444, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI157266292, Length=467, Percent_Identity=43.4689507494647, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI294660772, Length=419, Percent_Identity=42.7207637231504, Blast_Score=328, Evalue=8e-90,
Organism=Escherichia coli, GI48994877, Length=491, Percent_Identity=30.3462321792261, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320689, Length=356, Percent_Identity=30.6179775280899, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21358067, Length=525, Percent_Identity=41.3333333333333, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI21355149, Length=545, Percent_Identity=40.5504587155963, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI85725200, Length=471, Percent_Identity=42.0382165605096, Blast_Score=342, Evalue=4e-94,
Organism=Drosophila melanogaster, GI85815833, Length=471, Percent_Identity=42.0382165605096, Blast_Score=342, Evalue=4e-94,
Organism=Drosophila melanogaster, GI21355151, Length=493, Percent_Identity=40.973630831643, Blast_Score=338, Evalue=7e-93,
Organism=Drosophila melanogaster, GI21355981, Length=499, Percent_Identity=41.6833667334669, Blast_Score=334, Evalue=1e-91,
Organism=Drosophila melanogaster, GI19921912, Length=496, Percent_Identity=40.9274193548387, Blast_Score=326, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24657842, Length=487, Percent_Identity=38.8090349075975, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24651554, Length=531, Percent_Identity=38.9830508474576, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24657835, Length=489, Percent_Identity=39.4683026584867, Blast_Score=314, Evalue=8e-86,
Organism=Drosophila melanogaster, GI24657827, Length=473, Percent_Identity=39.5348837209302, Blast_Score=302, Evalue=5e-82,
Organism=Drosophila melanogaster, GI24585213, Length=501, Percent_Identity=38.12375249501, Blast_Score=300, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24651556, Length=467, Percent_Identity=40.2569593147752, Blast_Score=292, Evalue=4e-79,
Organism=Drosophila melanogaster, GI18859923, Length=491, Percent_Identity=35.234215885947, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24645593, Length=403, Percent_Identity=26.5508684863524, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 55955; Mature: 55955

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKLLVLSITAMLGLAACGDDGDKGADGNDGQDWTAVNQWFVDGQSRVTKAQELSVNNE
CCCCEEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHEEECCHHHHHHHHHCCCCCC
AGAAKNIILFVGDGMGVSTVTAARILEGQLKGQTGEENSLSFETLPYVGLAKTYNVDGQT
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCEECEEEECCCCCC
PDSAGTMTAMVTGVKTDVGVISQAEGVTRGNCASTDGQNLVTSLELAAMAGLSTGVVSTA
CCCCCCEEEEEECCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
RITHATPAAAYAHAPERNWESDADLPDEAITNGCSDIASQLLSFDYGAGLNVVMGGGRRA
EEECCCCHHHHCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCEEE
FIPNTVTDTEGKAGKRLDSRDLTSEWLTKYTNSAYVASRDEFLALDPATTDHALGLFNSS
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCEEEECCCCCCHHEEEECCC
HMEYELDRTTDGVTGEPSLAEMTAKSIDILQKNDKGFVLIIEAGRIDHAHHAGNAARALH
CCEEEECCCCCCCCCCCHHHHHHHHHHHHEEECCCCEEEEEECCCCCCHHHCCCHHHHHH
DTIALSEAVRVAMEKTSEKDTLLMVTADHSHVFTIAGYPTRGNPILGLVKGNGADGQPAV
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCCCCCEEEEEECCCCCCCCCC
TNATDANGLPYTTVGYANGLGYANLETGGDERYGYAAAPGRFDLNYVDTQSAGFHQEALV
CCCCCCCCCCEEEEECCCCCCEEECCCCCCCCCCEEECCCCEECEEECCCCCCCCHHHCC
PLGSETHAGEDVGIFASGPGAHLVQGTVEQNHLFHVMNHAGSLVEKAEAAQSTSN
CCCCCCCCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKKLLVLSITAMLGLAACGDDGDKGADGNDGQDWTAVNQWFVDGQSRVTKAQELSVNNE
CCCCEEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHEEECCHHHHHHHHHCCCCCC
AGAAKNIILFVGDGMGVSTVTAARILEGQLKGQTGEENSLSFETLPYVGLAKTYNVDGQT
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCEECEEEECCCCCC
PDSAGTMTAMVTGVKTDVGVISQAEGVTRGNCASTDGQNLVTSLELAAMAGLSTGVVSTA
CCCCCCEEEEEECCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
RITHATPAAAYAHAPERNWESDADLPDEAITNGCSDIASQLLSFDYGAGLNVVMGGGRRA
EEECCCCHHHHCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCEEE
FIPNTVTDTEGKAGKRLDSRDLTSEWLTKYTNSAYVASRDEFLALDPATTDHALGLFNSS
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCEEEECCCCCCHHEEEECCC
HMEYELDRTTDGVTGEPSLAEMTAKSIDILQKNDKGFVLIIEAGRIDHAHHAGNAARALH
CCEEEECCCCCCCCCCCHHHHHHHHHHHHEEECCCCEEEEEECCCCCCHHHCCCHHHHHH
DTIALSEAVRVAMEKTSEKDTLLMVTADHSHVFTIAGYPTRGNPILGLVKGNGADGQPAV
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCCCCCEEEEEECCCCCCCCCC
TNATDANGLPYTTVGYANGLGYANLETGGDERYGYAAAPGRFDLNYVDTQSAGFHQEALV
CCCCCCCCCCEEEEECCCCCCEEECCCCCCCCCCEEECCCCEECEEECCCCCCCCHHHCC
PLGSETHAGEDVGIFASGPGAHLVQGTVEQNHLFHVMNHAGSLVEKAEAAQSTSN
CCCCCCCCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]