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The map label for this gene is oprF [H]

Identifier: 157960903

GI number: 157960903

Start: 1299860

End: 1300957

Strand: Reverse

Name: oprF [H]

Synonym: Spea_1075

Alternate gene names: 157960903

Gene position: 1300957-1299860 (Counterclockwise)

Preceding gene: 157960904

Following gene: 157960902

Centisome position: 25.14

GC content: 46.17

Gene sequence:

>1098_bases
ATGATGAACAACACACTAAAAGCAGTTCTGCTTACTTCGATGTTACCTTTTGCTGCTAGCGCGGCTGATGGCATGAGCCC
CTGGTATGTAGGTGGCGGTGTCGGTATCAATAACTACCAACCCAATTGCGATCAAAAAACCATGAAAAGCTGTGGTGAGG
ACGACCCTTATGCATGGGACGTATTCGCAGGTTATTTATTTAATGACTACTTTGGTGCAGAGCTAGGTTATCGAGACCTA
GGTCGAGCTCAGTGGGTCGACTATTCAAACAAGCTAAATGACGTTGGTGCTAAAGGAATGAACCTTGGCCTAGTGGCATT
TTGGCCGTTTGCTGGTGATTGGAGCCTGTCTGCCGAAGCGGGTGCCTTTAACTACATCATCTCCAACAACAAGAACTATG
GTAGTGAATACTACAGCGATAATGGCGTCGCTCCATACGTAGGAGCAGGCATTGGTTATAACATTACCGATAACCTTAAA
ATCCAAGCTAAGTACCGTCGTTATGAAAACCTTGACGAAGATAAGTGGAAAACCCTAGAGATGGAAAGTAACTACTGGGG
GCTTGAGCTAAGTTATCGTTTTGGTAGCAAGTCAAAGCCTGCTCCGGTTGTAGCACCTGTAATAGTGGCAGACTCTGACA
ATGATGGCGTGACTGATGATATGGATCAGTGCCCTAATACACCATCGACTCACAAAGTGGATGCTAACGGTTGTACTATC
TATCAAGAAGCTGAAGACAAGTTTGATATCGAAGCTAAGTTCGCTACCAACTCCGCCACGGTACAAGAGTCATCATACGG
TGATATCAAGGAGCTTGCTGAATACATGAGTCAGCACCCTAATGCCGAAGTGACTATCTCTGGTCATGCTTCAAATACAG
GTAAAGCTGATTACAATATGGCGCTTTCTGAGCGCCGCGCCGCAGCGGTTGCCGAGATCCTTATCAATGAGTACGGGGTT
GCATCGAGCAAAGTATCATCTAAAGGCTACGGTATAACTCAGCCACTGATTGAAGGAAGCTCTGCAGAAGCAAACGAAGC
CAATCGCCGTATCGAAGCTCATGTTACCGGCACAGAAGAAGTGCCAGTACTTAAGTAA

Upstream 100 bases:

>100_bases
AGCTGGCTAAAGCATAAAAGGATCTCCGTGATAAGTGCTAGCCCTGCTTACATTGAATAAAAGCAGATGGAGAAGTTTTT
ATAAAACATAAGGATTTTGA

Downstream 100 bases:

>100_bases
TTCAGGTAAAAGTTAACAAAAGCCTATGGTTATCACAGCCATGGGCTTTTTTAATTTTATGCATTCATAAAAAAGCTATA
CTTCTTGATTAGACTTTTAT

Product: OmpA/MotB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MMNNTLKAVLLTSMLPFAASAADGMSPWYVGGGVGINNYQPNCDQKTMKSCGEDDPYAWDVFAGYLFNDYFGAELGYRDL
GRAQWVDYSNKLNDVGAKGMNLGLVAFWPFAGDWSLSAEAGAFNYIISNNKNYGSEYYSDNGVAPYVGAGIGYNITDNLK
IQAKYRRYENLDEDKWKTLEMESNYWGLELSYRFGSKSKPAPVVAPVIVADSDNDGVTDDMDQCPNTPSTHKVDANGCTI
YQEAEDKFDIEAKFATNSATVQESSYGDIKELAEYMSQHPNAEVTISGHASNTGKADYNMALSERRAAAVAEILINEYGV
ASSKVSSKGYGITQPLIEGSSAEANEANRRIEAHVTGTEEVPVLK

Sequences:

>Translated_365_residues
MMNNTLKAVLLTSMLPFAASAADGMSPWYVGGGVGINNYQPNCDQKTMKSCGEDDPYAWDVFAGYLFNDYFGAELGYRDL
GRAQWVDYSNKLNDVGAKGMNLGLVAFWPFAGDWSLSAEAGAFNYIISNNKNYGSEYYSDNGVAPYVGAGIGYNITDNLK
IQAKYRRYENLDEDKWKTLEMESNYWGLELSYRFGSKSKPAPVVAPVIVADSDNDGVTDDMDQCPNTPSTHKVDANGCTI
YQEAEDKFDIEAKFATNSATVQESSYGDIKELAEYMSQHPNAEVTISGHASNTGKADYNMALSERRAAAVAEILINEYGV
ASSKVSSKGYGITQPLIEGSSAEANEANRRIEAHVTGTEEVPVLK
>Mature_365_residues
MMNNTLKAVLLTSMLPFAASAADGMSPWYVGGGVGINNYQPNCDQKTMKSCGEDDPYAWDVFAGYLFNDYFGAELGYRDL
GRAQWVDYSNKLNDVGAKGMNLGLVAFWPFAGDWSLSAEAGAFNYIISNNKNYGSEYYSDNGVAPYVGAGIGYNITDNLK
IQAKYRRYENLDEDKWKTLEMESNYWGLELSYRFGSKSKPAPVVAPVIVADSDNDGVTDDMDQCPNTPSTHKVDANGCTI
YQEAEDKFDIEAKFATNSATVQESSYGDIKELAEYMSQHPNAEVTISGHASNTGKADYNMALSERRAAAVAEILINEYGV
ASSKVSSKGYGITQPLIEGSSAEANEANRRIEAHVTGTEEVPVLK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1787191, Length=359, Percent_Identity=23.6768802228412, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI48994946, Length=99, Percent_Identity=34.3434343434343, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1480 Molecules/Cell In: Growth Phase, Mi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 39880; Mature: 39880

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNNTLKAVLLTSMLPFAASAADGMSPWYVGGGVGINNYQPNCDQKTMKSCGEDDPYAWD
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHHHCCCCCCEEHH
VFAGYLFNDYFGAELGYRDLGRAQWVDYSNKLNDVGAKGMNLGLVAFWPFAGDWSLSAEA
HHHHHHHHHHHCCCCCHHHCCCHHCCCCCCHHHHCCCCCCCEEEEEEECCCCCCCCCCCC
GAFNYIISNNKNYGSEYYSDNGVAPYVGAGIGYNITDNLKIQAKYRRYENLDEDKWKTLE
CEEEEEEECCCCCCCHHCCCCCCCCEECCCCCCCCCCCEEEEEEEHHHCCCCCCCCEEEE
MESNYWGLELSYRFGSKSKPAPVVAPVIVADSDNDGVTDDMDQCPNTPSTHKVDANGCTI
ECCCEEEEEEEEECCCCCCCCCEEEEEEEEECCCCCCCCHHHHCCCCCCCEEECCCCCEE
YQEAEDKFDIEAKFATNSATVQESSYGDIKELAEYMSQHPNAEVTISGHASNTGKADYNM
EECCCCCEEEEEEEECCCCEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHH
ALSERRAAAVAEILINEYGVASSKVSSKGYGITQPLIEGSSAEANEANRRIEAHVTGTEE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCC
VPVLK
CCCCC
>Mature Secondary Structure
MMNNTLKAVLLTSMLPFAASAADGMSPWYVGGGVGINNYQPNCDQKTMKSCGEDDPYAWD
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHHHCCCCCCEEHH
VFAGYLFNDYFGAELGYRDLGRAQWVDYSNKLNDVGAKGMNLGLVAFWPFAGDWSLSAEA
HHHHHHHHHHHCCCCCHHHCCCHHCCCCCCHHHHCCCCCCCEEEEEEECCCCCCCCCCCC
GAFNYIISNNKNYGSEYYSDNGVAPYVGAGIGYNITDNLKIQAKYRRYENLDEDKWKTLE
CEEEEEEECCCCCCCHHCCCCCCCCEECCCCCCCCCCCEEEEEEEHHHCCCCCCCCEEEE
MESNYWGLELSYRFGSKSKPAPVVAPVIVADSDNDGVTDDMDQCPNTPSTHKVDANGCTI
ECCCEEEEEEEEECCCCCCCCCEEEEEEEEECCCCCCCCHHHHCCCCCCCEEECCCCCEE
YQEAEDKFDIEAKFATNSATVQESSYGDIKELAEYMSQHPNAEVTISGHASNTGKADYNM
EECCCCCEEEEEEEECCCCEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHH
ALSERRAAAVAEILINEYGVASSKVSSKGYGITQPLIEGSSAEANEANRRIEAHVTGTEE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCC
VPVLK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]