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The map label for this gene is pgk [H]

Identifier: 157960618

GI number: 157960618

Start: 977642

End: 978817

Strand: Direct

Name: pgk [H]

Synonym: Spea_0790

Alternate gene names: 157960618

Gene position: 977642-978817 (Clockwise)

Preceding gene: 157960617

Following gene: 157960619

Centisome position: 18.89

GC content: 50.17

Gene sequence:

>1176_bases
ATGACGATTCTTAATATGAAAGATTTAGACCTTCAAGGTAAGCGTGTGCTTATTCGTGAAGATCTTAACGTACCAGTCAG
CGATGGTGTGGTAACCAGTGACGCGCGTCTGCGTGCTTCTCTACCAACGATTAAGCTTGCGCTTGAAAAAGGTGCGGCGG
TTATGGTGATGTCTCACCTAGGCCGTCCAACTGAAGGCGAGTTCAATGCTGAGTTCTCTATGCAGCCTGTAGTTGATTAC
CTAACAAAGGCGCTAGATTGCCCTGTACGCCTAGCAGCAGACTACCTAGACGGCATCGAAGTGGCTGTCGGTGAAGTGGT
AGTGTTTGAAAACGTGCGTTTTAACTTGGGTGAGAAGAAGAACGACGAAGCGCTGGCTAAGAAGATGGCGGCACTTTGTG
ACGTATACGTGATGGATGCATTCGGCACGGCTCACCGCGCACAAGCATCGACTCACGGTGTTGGTCTGCACGCGCCTATC
GCTTGTGCGGGTCCATTGTTAGCGGGTGAGTTAGAAGCCTTAGGTAAGGCTCTTGATAACCCAGCACGCCCAATGGTAGC
CATTGTTGGTGGTTCTAAAGTTTCAACAAAACTGACAGTGCTTGAAAGCCTTTCAACTAAAGTTGACCAACTTGTAGTGG
GTGGCGGCATCGCCAACACTTTCGTTGCTGCAGCAGGCCACAAGGTGGGTAAGTCGCTTTATGAGGCCGACCTGATTGAA
GAAGCTCAACGCCTTGTTGCTAATGCGCAGAGCCGTGGTGGTGACATTCCAGTACCTACTGATGTGGTTGTTGCAGGCGA
GTTCAGCCCTACCGCAACTGCAACGCTGAAAGATGTAAGCGATGTATCTGACACAGATATGATTTTCGATATCGGCCCAG
ATAGCGCCGAAGCTTTAGCCGAGATCATCAAAAATGCTGGCACGGTTGTATGGAACGGTCCAGTCGGTGTATTTGAGTTT
GATCAGTTCGGTGAAGGGACTAAGCGTATTGCTCAAGCAATTGCCGAGTCAAATGCCTTCTCTATCGCTGGCGGTGGTGA
CACATTAGCGGCTGTCGATAAGTATGGTATCGCCGACAAGGTGTCTTATATCTCAACGGGTGGCGGCGCTTTCCTAGAGT
TTTTAGAAGGAAAAGAGTTGCCAGCAGTTGCCATGCTTGAGAGCCGCGGCCAGTAA

Upstream 100 bases:

>100_bases
GTGTTGTGTCCCGGTGGTCGGGTAATATCGTTTTATTGAATTTTGCTCAACTTAAATTTTTTAACTTTAATTTTAACTTA
GATAGTAAGGAAGCATGAAA

Downstream 100 bases:

>100_bases
TCGACAACTATACTAGTGGCAACATACAAAAAAATTACCAAACTGGTTGTTGGCGGGCTTTAAATCGACATTTGTCAGAG
TTACACTAGCCGCCAACAAA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTILNMKDLDLQGKRVLIREDLNVPVSDGVVTSDARLRASLPTIKLALEKGAAVMVMSHLGRPTEGEFNAEFSMQPVVDY
LTKALDCPVRLAADYLDGIEVAVGEVVVFENVRFNLGEKKNDEALAKKMAALCDVYVMDAFGTAHRAQASTHGVGLHAPI
ACAGPLLAGELEALGKALDNPARPMVAIVGGSKVSTKLTVLESLSTKVDQLVVGGGIANTFVAAAGHKVGKSLYEADLIE
EAQRLVANAQSRGGDIPVPTDVVVAGEFSPTATATLKDVSDVSDTDMIFDIGPDSAEALAEIIKNAGTVVWNGPVGVFEF
DQFGEGTKRIAQAIAESNAFSIAGGGDTLAAVDKYGIADKVSYISTGGGAFLEFLEGKELPAVAMLESRGQ

Sequences:

>Translated_391_residues
MTILNMKDLDLQGKRVLIREDLNVPVSDGVVTSDARLRASLPTIKLALEKGAAVMVMSHLGRPTEGEFNAEFSMQPVVDY
LTKALDCPVRLAADYLDGIEVAVGEVVVFENVRFNLGEKKNDEALAKKMAALCDVYVMDAFGTAHRAQASTHGVGLHAPI
ACAGPLLAGELEALGKALDNPARPMVAIVGGSKVSTKLTVLESLSTKVDQLVVGGGIANTFVAAAGHKVGKSLYEADLIE
EAQRLVANAQSRGGDIPVPTDVVVAGEFSPTATATLKDVSDVSDTDMIFDIGPDSAEALAEIIKNAGTVVWNGPVGVFEF
DQFGEGTKRIAQAIAESNAFSIAGGGDTLAAVDKYGIADKVSYISTGGGAFLEFLEGKELPAVAMLESRGQ
>Mature_390_residues
TILNMKDLDLQGKRVLIREDLNVPVSDGVVTSDARLRASLPTIKLALEKGAAVMVMSHLGRPTEGEFNAEFSMQPVVDYL
TKALDCPVRLAADYLDGIEVAVGEVVVFENVRFNLGEKKNDEALAKKMAALCDVYVMDAFGTAHRAQASTHGVGLHAPIA
CAGPLLAGELEALGKALDNPARPMVAIVGGSKVSTKLTVLESLSTKVDQLVVGGGIANTFVAAAGHKVGKSLYEADLIEE
AQRLVANAQSRGGDIPVPTDVVVAGEFSPTATATLKDVSDVSDTDMIFDIGPDSAEALAEIIKNAGTVVWNGPVGVFEFD
QFGEGTKRIAQAIAESNAFSIAGGGDTLAAVDKYGIADKVSYISTGGGAFLEFLEGKELPAVAMLESRGQ

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI31543397, Length=413, Percent_Identity=39.225181598063, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI4505763, Length=413, Percent_Identity=38.498789346247, Blast_Score=263, Evalue=3e-70,
Organism=Escherichia coli, GI1789294, Length=391, Percent_Identity=72.1227621483376, Blast_Score=565, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI17508823, Length=414, Percent_Identity=42.9951690821256, Blast_Score=296, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6319857, Length=412, Percent_Identity=40.7766990291262, Blast_Score=284, Evalue=1e-77,
Organism=Drosophila melanogaster, GI17136394, Length=413, Percent_Identity=41.1622276029056, Blast_Score=266, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24581203, Length=411, Percent_Identity=38.9294403892944, Blast_Score=251, Evalue=7e-67,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 40876; Mature: 40745

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILNMKDLDLQGKRVLIREDLNVPVSDGVVTSDARLRASLPTIKLALEKGAAVMVMSHL
CEEECCCCCCCCCCEEEEEECCCCCCCCCEEECCCHHEECCCEEEEEHHCCCEEEHHHHC
GRPTEGEFNAEFSMQPVVDYLTKALDCPVRLAADYLDGIEVAVGEVVVFENVRFNLGEKK
CCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHCCEEEEECCEECCCCCC
NDEALAKKMAALCDVYVMDAFGTAHRAQASTHGVGLHAPIACAGPLLAGELEALGKALDN
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCC
PARPMVAIVGGSKVSTKLTVLESLSTKVDQLVVGGGIANTFVAAAGHKVGKSLYEADLIE
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
EAQRLVANAQSRGGDIPVPTDVVVAGEFSPTATATLKDVSDVSDTDMIFDIGPDSAEALA
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCCEEEEECCCHHHHHH
EIIKNAGTVVWNGPVGVFEFDQFGEGTKRIAQAIAESNAFSIAGGGDTLAAVDKYGIADK
HHHHCCCEEEECCCCCEEECHHCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCHHH
VSYISTGGGAFLEFLEGKELPAVAMLESRGQ
HHHEECCCHHHHHHHCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
TILNMKDLDLQGKRVLIREDLNVPVSDGVVTSDARLRASLPTIKLALEKGAAVMVMSHL
EEECCCCCCCCCCEEEEEECCCCCCCCCEEECCCHHEECCCEEEEEHHCCCEEEHHHHC
GRPTEGEFNAEFSMQPVVDYLTKALDCPVRLAADYLDGIEVAVGEVVVFENVRFNLGEKK
CCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHCCEEEEECCEECCCCCC
NDEALAKKMAALCDVYVMDAFGTAHRAQASTHGVGLHAPIACAGPLLAGELEALGKALDN
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCC
PARPMVAIVGGSKVSTKLTVLESLSTKVDQLVVGGGIANTFVAAAGHKVGKSLYEADLIE
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
EAQRLVANAQSRGGDIPVPTDVVVAGEFSPTATATLKDVSDVSDTDMIFDIGPDSAEALA
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCCEEEEECCCHHHHHH
EIIKNAGTVVWNGPVGVFEFDQFGEGTKRIAQAIAESNAFSIAGGGDTLAAVDKYGIADK
HHHHCCCEEEECCCCCEEECHHCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCHHH
VSYISTGGGAFLEFLEGKELPAVAMLESRGQ
HHHEECCCHHHHHHHCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA