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The map label for this gene is ysgA [C]

Identifier: 157960571

GI number: 157960571

Start: 920124

End: 920861

Strand: Direct

Name: ysgA [C]

Synonym: Spea_0743

Alternate gene names: 157960571

Gene position: 920124-920861 (Clockwise)

Preceding gene: 157960570

Following gene: 157960573

Centisome position: 17.78

GC content: 48.51

Gene sequence:

>738_bases
ATGATTATTACTCAAGAAGTCCATGATATTCCCACCGCGACAGGCAGCATGCGAACCTACCTATACCGCCCCGACAGTGA
AGGGCAATTCGCGACTATTATTTTTTACTCTGAGATCTTTCAGCAGACGGCACCGATCGCGCGTGCGGCAGCTATTCTCG
CAGGCCATGGCTTTGTGGTCTTAGTGCCAGAGGTATTTCATGAGCTAAACCCTGTTGGCACCGTGCTTGAGTACGATGAT
GTCGGTAAAGATAAAGGTAACGCCGATAAGTTTGCTAAGCCGCTTGAACACCATGACAGTGATACTGAGGCGCTGGTGGA
CTTTGCTCGCAGCTTAAGTTTTTGCAGCAGTCACATTGGCAGCATGGGCGTATGTATTGGCGGTCACTTAGCCTATCGCG
CGGCGCTTAATCCTGATATTCAAGGGGCCTTCTGCTTATACGCAACAGATATCCATTCTAATACGCTACCTTGTGATAAA
GGCAACGATTCCTTGAGCCGCACCGGCGATATCGCAGCCGAGCTAGTGATGGTGTTTGGTAAGCAAGATCCTCATGTTTC
CAGTGAAGGGCGTAAAGCTATCTATAACAAACTTGAAGCCACTGAACGCAAGTTTAATTGGCTTGAGGTGAATGCTCAGC
ACGCCTTTATGCGGGATGGGAGTGATAGGTATGATCCAGCGTTAGCTATTCAGATGTATCTGCAGGCGGTAGCGTTTTTT
CAGCGAACACTTAAGTAA

Upstream 100 bases:

>100_bases
AGAGGCTTTGAGCGCGATTGCCCATACGACACTGACAAATGTCACCCAGCTTTTAGCCGGAGAGCATTGTCCTAACGAGC
TGTATTAGGAAGGAAAGCCG

Downstream 100 bases:

>100_bases
AACGCTTGAGTAATGTTTTGAATAAAATACGTTAATCAATGCAAATAAAAAGGGCCGATATAATCGGCCCTTTTATCTAT
CACGGAGTAACTAATATTAA

Product: dienelactone hydrolase

Products: oxohex-2-enedioate [C]

Alternate protein names: Dienelactone Hydrolase; Dienelactone Hydrolase Family Protein; Dienelactone Hydrolase Family; Hydrolase; Dienelactone Hydrolase Or ; Carboxymethylenebutenolidase Family Protein; Threonyl-TRNA Synthetase Class IIA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MIITQEVHDIPTATGSMRTYLYRPDSEGQFATIIFYSEIFQQTAPIARAAAILAGHGFVVLVPEVFHELNPVGTVLEYDD
VGKDKGNADKFAKPLEHHDSDTEALVDFARSLSFCSSHIGSMGVCIGGHLAYRAALNPDIQGAFCLYATDIHSNTLPCDK
GNDSLSRTGDIAAELVMVFGKQDPHVSSEGRKAIYNKLEATERKFNWLEVNAQHAFMRDGSDRYDPALAIQMYLQAVAFF
QRTLK

Sequences:

>Translated_245_residues
MIITQEVHDIPTATGSMRTYLYRPDSEGQFATIIFYSEIFQQTAPIARAAAILAGHGFVVLVPEVFHELNPVGTVLEYDD
VGKDKGNADKFAKPLEHHDSDTEALVDFARSLSFCSSHIGSMGVCIGGHLAYRAALNPDIQGAFCLYATDIHSNTLPCDK
GNDSLSRTGDIAAELVMVFGKQDPHVSSEGRKAIYNKLEATERKFNWLEVNAQHAFMRDGSDRYDPALAIQMYLQAVAFF
QRTLK
>Mature_245_residues
MIITQEVHDIPTATGSMRTYLYRPDSEGQFATIIFYSEIFQQTAPIARAAAILAGHGFVVLVPEVFHELNPVGTVLEYDD
VGKDKGNADKFAKPLEHHDSDTEALVDFARSLSFCSSHIGSMGVCIGGHLAYRAALNPDIQGAFCLYATDIHSNTLPCDK
GNDSLSRTGDIAAELVMVFGKQDPHVSSEGRKAIYNKLEATERKFNWLEVNAQHAFMRDGSDRYDPALAIQMYLQAVAFF
QRTLK

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Saccharomyces cerevisiae, GI6320117, Length=242, Percent_Identity=39.6694214876033, Blast_Score=163, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.1.1.45 [C]

Molecular weight: Translated: 27064; Mature: 27064

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIITQEVHDIPTATGSMRTYLYRPDSEGQFATIIFYSEIFQQTAPIARAAAILAGHGFVV
CCCCCCHHHCCCCCCCCEEEEECCCCCCCEEEEEEHHHHHHHHCCHHHHHHHHHCCCEEE
LVPEVFHELNPVGTVLEYDDVGKDKGNADKFAKPLEHHDSDTEALVDFARSLSFCSSHIG
ECHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
SMGVCIGGHLAYRAALNPDIQGAFCLYATDIHSNTLPCDKGNDSLSRTGDIAAELVMVFG
CCCEEECCHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
KQDPHVSSEGRKAIYNKLEATERKFNWLEVNAQHAFMRDGSDRYDPALAIQMYLQAVAFF
CCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCHHHHHCCCCCCCCHHHHHHHHHHHHHHH
QRTLK
HHHCC
>Mature Secondary Structure
MIITQEVHDIPTATGSMRTYLYRPDSEGQFATIIFYSEIFQQTAPIARAAAILAGHGFVV
CCCCCCHHHCCCCCCCCEEEEECCCCCCCEEEEEEHHHHHHHHCCHHHHHHHHHCCCEEE
LVPEVFHELNPVGTVLEYDDVGKDKGNADKFAKPLEHHDSDTEALVDFARSLSFCSSHIG
ECHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
SMGVCIGGHLAYRAALNPDIQGAFCLYATDIHSNTLPCDKGNDSLSRTGDIAAELVMVFG
CCCEEECCHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
KQDPHVSSEGRKAIYNKLEATERKFNWLEVNAQHAFMRDGSDRYDPALAIQMYLQAVAFF
CCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCHHHHHCCCCCCCCHHHHHHHHHHHHHHH
QRTLK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-carboxymethylenebut-2-en-4-olide; H2O [C]

Specific reaction: 4-carboxymethylenebut-2-en-4-olide + H2O = 4 oxohex-2-enedioate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA