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The map label for this gene is ldhA [H]

Identifier: 157960570

GI number: 157960570

Start: 919122

End: 920111

Strand: Direct

Name: ldhA [H]

Synonym: Spea_0742

Alternate gene names: 157960570

Gene position: 919122-920111 (Clockwise)

Preceding gene: 157960564

Following gene: 157960571

Centisome position: 17.76

GC content: 47.07

Gene sequence:

>990_bases
ATGAAAATAGGCTTCTTTAGCGCTAAACATTACGACATGCAGTATTTCGATCGAACTAATGCCGCTTTCAGCGCCAAGAT
TGAGTACTTCGATTATCGCCTTTGCATGCAAACGGTTAAGTTAGCTGAAGGCTTTGAGATCGTGTGCGCCTTCGTCAATG
ATTCACTGTGTGAAGAGGTGTTGGTGGAGCTAGCACAAGGTGGAACCAAGATTATTGCCATGCGTTGTGCTGGTTTTAAC
AATGTTGACTTAGAAGCTGCCGAGCGCCTAGGCATGAAGGTAGTCAATGTGCCTGCCTATTCTCCTGAGTCAGTTGCCGA
GCATACGGTAGCACTCATGCTGACCCTAAACCGTAAAATTCATAAAGCCTATCAGCGCACGCGAGATGCGAACTTTTCTT
TAGATGGCTTAGTCGGCTTTAACATGCATGGCCGCACCGTTGGCGTGATAGGCACGGGTAAGATTGGTCTAGCAACCATC
AAGGTGCTACAAGGCTTTGGTTGCAAGGTTGTGGCTCACGATCCCTACCCGAATCAAGCGGTTATTGATTTAGGTGTCGA
GTACTTGTCACTTGGGCAGCTTTATCCCCTCTGTGATGTGATTAGCCTGCATTGCCCATTAACTCAAGATAATCACCATC
TGCTCAGTGAAAACAGTTTTAGTCAAATGAAACCCGGTGTCATGGTAATCAACACTAGCCGTGGTGGTCTGCTTAACGCC
CTCGATGCGATGGAAGCGCTTAAATCTGGTCAGTTAGGCTCACTGGGCTTAGATGTTTACGAGAATGAGAAAGAGCTTTT
CTTTGAAGATAAGTCGAGCGAGATCATTCAAGATGATGTGTTTAGACGCCTGTCGGCGTGCCATAACGTGATCTTTACTG
GCCATCAGGCATTCTTAACCGAAGAGGCTTTGAGCGCGATTGCCCATACGACACTGACAAATGTCACCCAGCTTTTAGCC
GGAGAGCATTGTCCTAACGAGCTGTATTAG

Upstream 100 bases:

>100_bases
TGAGCTAAGCGGCAAAAAATAACCCCAAGATGGCTACTTTATAATAGCGATTGGTATACACTGTTTAAGTTTCTCTCCTC
ATTTAGATAAGGTTTCTACA

Downstream 100 bases:

>100_bases
GAAGGAAAGCCGATGATTATTACTCAAGAAGTCCATGATATTCCCACCGCGACAGGCAGCATGCGAACCTACCTATACCG
CCCCGACAGTGAAGGGCAAT

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKIGFFSAKHYDMQYFDRTNAAFSAKIEYFDYRLCMQTVKLAEGFEIVCAFVNDSLCEEVLVELAQGGTKIIAMRCAGFN
NVDLEAAERLGMKVVNVPAYSPESVAEHTVALMLTLNRKIHKAYQRTRDANFSLDGLVGFNMHGRTVGVIGTGKIGLATI
KVLQGFGCKVVAHDPYPNQAVIDLGVEYLSLGQLYPLCDVISLHCPLTQDNHHLLSENSFSQMKPGVMVINTSRGGLLNA
LDAMEALKSGQLGSLGLDVYENEKELFFEDKSSEIIQDDVFRRLSACHNVIFTGHQAFLTEEALSAIAHTTLTNVTQLLA
GEHCPNELY

Sequences:

>Translated_329_residues
MKIGFFSAKHYDMQYFDRTNAAFSAKIEYFDYRLCMQTVKLAEGFEIVCAFVNDSLCEEVLVELAQGGTKIIAMRCAGFN
NVDLEAAERLGMKVVNVPAYSPESVAEHTVALMLTLNRKIHKAYQRTRDANFSLDGLVGFNMHGRTVGVIGTGKIGLATI
KVLQGFGCKVVAHDPYPNQAVIDLGVEYLSLGQLYPLCDVISLHCPLTQDNHHLLSENSFSQMKPGVMVINTSRGGLLNA
LDAMEALKSGQLGSLGLDVYENEKELFFEDKSSEIIQDDVFRRLSACHNVIFTGHQAFLTEEALSAIAHTTLTNVTQLLA
GEHCPNELY
>Mature_329_residues
MKIGFFSAKHYDMQYFDRTNAAFSAKIEYFDYRLCMQTVKLAEGFEIVCAFVNDSLCEEVLVELAQGGTKIIAMRCAGFN
NVDLEAAERLGMKVVNVPAYSPESVAEHTVALMLTLNRKIHKAYQRTRDANFSLDGLVGFNMHGRTVGVIGTGKIGLATI
KVLQGFGCKVVAHDPYPNQAVIDLGVEYLSLGQLYPLCDVISLHCPLTQDNHHLLSENSFSQMKPGVMVINTSRGGLLNA
LDAMEALKSGQLGSLGLDVYENEKELFFEDKSSEIIQDDVFRRLSACHNVIFTGHQAFLTEEALSAIAHTTLTNVTQLLA
GEHCPNELY

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=195, Percent_Identity=36.4102564102564, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI145580578, Length=262, Percent_Identity=30.1526717557252, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI4557499, Length=262, Percent_Identity=30.1526717557252, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI61743967, Length=243, Percent_Identity=32.9218106995885, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI4557497, Length=243, Percent_Identity=32.9218106995885, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI145580575, Length=262, Percent_Identity=30.1526717557252, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI6912396, Length=277, Percent_Identity=26.7148014440433, Blast_Score=98, Evalue=9e-21,
Organism=Escherichia coli, GI1787645, Length=328, Percent_Identity=61.5853658536585, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1789279, Length=228, Percent_Identity=28.9473684210526, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082289, Length=191, Percent_Identity=27.2251308900524, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1788660, Length=222, Percent_Identity=29.7297297297297, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=210, Percent_Identity=32.3809523809524, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI25147481, Length=260, Percent_Identity=29.2307692307692, Blast_Score=111, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6324055, Length=199, Percent_Identity=29.6482412060301, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320925, Length=238, Percent_Identity=29.8319327731092, Blast_Score=99, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6322116, Length=250, Percent_Identity=28.4, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324964, Length=204, Percent_Identity=28.4313725490196, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=27.1428571428571, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24646446, Length=275, Percent_Identity=32.3636363636364, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24646448, Length=275, Percent_Identity=32.3636363636364, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24646452, Length=275, Percent_Identity=32.3636363636364, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24646450, Length=275, Percent_Identity=32.3636363636364, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI62472511, Length=275, Percent_Identity=32.3636363636364, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI19921140, Length=265, Percent_Identity=26.7924528301887, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI28574286, Length=283, Percent_Identity=27.5618374558304, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI45552429, Length=262, Percent_Identity=27.0992366412214, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585514, Length=269, Percent_Identity=26.7657992565056, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28574282, Length=269, Percent_Identity=26.7657992565056, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28574284, Length=269, Percent_Identity=26.7657992565056, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45551003, Length=269, Percent_Identity=26.7657992565056, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28571528, Length=300, Percent_Identity=24, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24585516, Length=207, Percent_Identity=24.1545893719807, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36297; Mature: 36297

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGFFSAKHYDMQYFDRTNAAFSAKIEYFDYRLCMQTVKLAEGFEIVCAFVNDSLCEEV
CEEECCCCCCCCHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEEEHHCHHHHHHH
LVELAQGGTKIIAMRCAGFNNVDLEAAERLGMKVVNVPAYSPESVAEHTVALMLTLNRKI
HHHHHCCCCEEEEEEECCCCCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
HKAYQRTRDANFSLDGLVGFNMHGRTVGVIGTGKIGLATIKVLQGFGCKVVAHDPYPNQA
HHHHHHHCCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHCCCCEEEECCCCCCCH
VIDLGVEYLSLGQLYPLCDVISLHCPLTQDNHHLLSENSFSQMKPGVMVINTSRGGLLNA
HHHHCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCEEEEECCCCCHHHH
LDAMEALKSGQLGSLGLDVYENEKELFFEDKSSEIIQDDVFRRLSACHNVIFTGHQAFLT
HHHHHHHHCCCCCCCCCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHEECCCHHHHH
EEALSAIAHTTLTNVTQLLAGEHCPNELY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKIGFFSAKHYDMQYFDRTNAAFSAKIEYFDYRLCMQTVKLAEGFEIVCAFVNDSLCEEV
CEEECCCCCCCCHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEEEHHCHHHHHHH
LVELAQGGTKIIAMRCAGFNNVDLEAAERLGMKVVNVPAYSPESVAEHTVALMLTLNRKI
HHHHHCCCCEEEEEEECCCCCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
HKAYQRTRDANFSLDGLVGFNMHGRTVGVIGTGKIGLATIKVLQGFGCKVVAHDPYPNQA
HHHHHHHCCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHCCCCEEEECCCCCCCH
VIDLGVEYLSLGQLYPLCDVISLHCPLTQDNHHLLSENSFSQMKPGVMVINTSRGGLLNA
HHHHCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCEEEEECCCCCHHHH
LDAMEALKSGQLGSLGLDVYENEKELFFEDKSSEIIQDDVFRRLSACHNVIFTGHQAFLT
HHHHHHHHCCCCCCCCCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHEECCCHHHHH
EEALSAIAHTTLTNVTQLLAGEHCPNELY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]