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The map label for this gene is rfbD [H]

Identifier: 157960380

GI number: 157960380

Start: 677716

End: 678639

Strand: Direct

Name: rfbD [H]

Synonym: Spea_0551

Alternate gene names: 157960380

Gene position: 677716-678639 (Clockwise)

Preceding gene: 157960378

Following gene: 157960381

Centisome position: 13.1

GC content: 50.32

Gene sequence:

>924_bases
ATGGTAACTATGGCAAAGATTATGGTGACGGGCGCAACAGGTTTATTGGGGCGGGCCGTGGTCAAGCAGCTAAAGCAGTG
CAGTGATTTTCAGGTAATAGAGTGTGGATTTAGTCGAGCGAGTATCGGTATTGAAGTGCTAGATCTCACTCAGTCGGAGC
AGGTATTTGAATTCGTGGCTAAACACAAACCCGATGCCATAGTACACTGCGCCGCAGAGCGTCGCCCCGATGTGTCTGAG
CAAGCCCCTCAGGCCGCATTAGCTCTAAACGCGGAGGCTAGCGACTCGTTAGCAAAAGCCGCCAGCCAAAATGGGGCCTG
GCTGTTATACATCTCCACCGACTATGTGTTTGATGGCACAGCCCCTAAATATGAAGAGTCGAGTCAGACTAATCCTGTTA
ACTTTTACGGCCAGTCAAAACTACAAGGGGAGAACCTTGTGGCTGATTCTGAGCCTAGTTTTGCTATTTTGCGCCTGCCG
ATCCTCTATGGAGATGTGGAAAGCCTAAACGAATCCGCAGTTATGGTGTTACTCAAGCATTTACTCGCCCCTCATGTGGA
GTACCTCGATGACTGGGCCGTAAGAAGCCCAACTTCAACGGCAGATATCGCCGTCGCCATCCACGAGATGTTAGTGTTGC
AGCTAGCTGGAAAGCAGCTCTCTGGGCGTTATCACTTTAGTGCCGAGGAGACCATGAGTAAGTATCAGATGCTATTAGTC
ATGGCAGAGATCCTCGGGCTAGACACGGGACACCTCATTGCTGTGACATCCCCCACAGATAGCGCTAAACGTCCAAAGGA
CTGCAGCCTAAGCTGTGAGCGACTTGCCAGTTTAGGGATTAAAAGCAGGCTACCTTTTAAGGTCGGAATAGAGAGCGCCT
TGTTGCAGTCTTCAGTTGTACAGCAATGGCTTGCTCAAAGATGA

Upstream 100 bases:

>100_bases
TTTAGTAAAGTAGGCTTTTTCAAGAGTAAACCAGTGCTTAGAGGATGAAAACCCCTTAAGCTAGGTGACTGACTTTTTGG
CCCATTTTATGGATTAATGG

Downstream 100 bases:

>100_bases
AAACGATAACAATAAAGAATGATTACCCATGATAACAATGAGCCCCATGATGAATACACGTTTTAAAAATCTACTGATAC
GTAATCTTGGGGCAACCGAT

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MVTMAKIMVTGATGLLGRAVVKQLKQCSDFQVIECGFSRASIGIEVLDLTQSEQVFEFVAKHKPDAIVHCAAERRPDVSE
QAPQAALALNAEASDSLAKAASQNGAWLLYISTDYVFDGTAPKYEESSQTNPVNFYGQSKLQGENLVADSEPSFAILRLP
ILYGDVESLNESAVMVLLKHLLAPHVEYLDDWAVRSPTSTADIAVAIHEMLVLQLAGKQLSGRYHFSAEETMSKYQMLLV
MAEILGLDTGHLIAVTSPTDSAKRPKDCSLSCERLASLGIKSRLPFKVGIESALLQSSVVQQWLAQR

Sequences:

>Translated_307_residues
MVTMAKIMVTGATGLLGRAVVKQLKQCSDFQVIECGFSRASIGIEVLDLTQSEQVFEFVAKHKPDAIVHCAAERRPDVSE
QAPQAALALNAEASDSLAKAASQNGAWLLYISTDYVFDGTAPKYEESSQTNPVNFYGQSKLQGENLVADSEPSFAILRLP
ILYGDVESLNESAVMVLLKHLLAPHVEYLDDWAVRSPTSTADIAVAIHEMLVLQLAGKQLSGRYHFSAEETMSKYQMLLV
MAEILGLDTGHLIAVTSPTDSAKRPKDCSLSCERLASLGIKSRLPFKVGIESALLQSSVVQQWLAQR
>Mature_307_residues
MVTMAKIMVTGATGLLGRAVVKQLKQCSDFQVIECGFSRASIGIEVLDLTQSEQVFEFVAKHKPDAIVHCAAERRPDVSE
QAPQAALALNAEASDSLAKAASQNGAWLLYISTDYVFDGTAPKYEESSQTNPVNFYGQSKLQGENLVADSEPSFAILRLP
ILYGDVESLNESAVMVLLKHLLAPHVEYLDDWAVRSPTSTADIAVAIHEMLVLQLAGKQLSGRYHFSAEETMSKYQMLLV
MAEILGLDTGHLIAVTSPTDSAKRPKDCSLSCERLASLGIKSRLPFKVGIESALLQSSVVQQWLAQR

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=300, Percent_Identity=42.3333333333333, Blast_Score=255, Evalue=3e-68,
Organism=Homo sapiens, GI11034825, Length=300, Percent_Identity=42.3333333333333, Blast_Score=255, Evalue=3e-68,
Organism=Escherichia coli, GI1788352, Length=284, Percent_Identity=26.7605633802817, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 33415; Mature: 33415

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTMAKIMVTGATGLLGRAVVKQLKQCSDFQVIECGFSRASIGIEVLDLTQSEQVFEFVA
CCEEEEEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCHHCCEEEEECCCHHHHHHHHH
KHKPDAIVHCAAERRPDVSEQAPQAALALNAEASDSLAKAASQNGAWLLYISTDYVFDGT
HCCCCCEEEECHHCCCCCHHHCCCCEEEECCCCHHHHHHHHCCCCCEEEEEEECEEECCC
APKYEESSQTNPVNFYGQSKLQGENLVADSEPSFAILRLPILYGDVESLNESAVMVLLKH
CCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEEEEHCCCHHHCCHHHHHHHHHH
LLAPHVEYLDDWAVRSPTSTADIAVAIHEMLVLQLAGKQLSGRYHFSAEETMSKYQMLLV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHCCEEECCHHHHHHHHHHHHH
MAEILGLDTGHLIAVTSPTDSAKRPKDCSLSCERLASLGIKSRLPFKVGIESALLQSSVV
HHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
QQWLAQR
HHHHHCC
>Mature Secondary Structure
MVTMAKIMVTGATGLLGRAVVKQLKQCSDFQVIECGFSRASIGIEVLDLTQSEQVFEFVA
CCEEEEEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCHHCCEEEEECCCHHHHHHHHH
KHKPDAIVHCAAERRPDVSEQAPQAALALNAEASDSLAKAASQNGAWLLYISTDYVFDGT
HCCCCCEEEECHHCCCCCHHHCCCCEEEECCCCHHHHHHHHCCCCCEEEEEEECEEECCC
APKYEESSQTNPVNFYGQSKLQGENLVADSEPSFAILRLPILYGDVESLNESAVMVLLKH
CCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEEEEHCCCHHHCCHHHHHHHHHH
LLAPHVEYLDDWAVRSPTSTADIAVAIHEMLVLQLAGKQLSGRYHFSAEETMSKYQMLLV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHCCEEECCHHHHHHHHHHHHH
MAEILGLDTGHLIAVTSPTDSAKRPKDCSLSCERLASLGIKSRLPFKVGIESALLQSSVV
HHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
QQWLAQR
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]