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The map label for this gene is aceF [H]

Identifier: 157960249

GI number: 157960249

Start: 514693

End: 516555

Strand: Direct

Name: aceF [H]

Synonym: Spea_0420

Alternate gene names: 157960249

Gene position: 514693-516555 (Clockwise)

Preceding gene: 157960248

Following gene: 157960250

Centisome position: 9.95

GC content: 46.65

Gene sequence:

>1863_bases
ATGACAATCGAAATTAATGTACCAGATATCGGTGCGGATGAGGTTGAAGTAACAGAGATCCTTGTTAGCGTAGGCGACAA
GGTTGAAGAAGAGCAATCGCTTATCTCTGTTGAAGGCGATAAAGCTGCGATGGAAGTTCCGGCTTCTGCAGCGGGTATTG
TTAAAGAAATTAAAGTAGTAGTAGGTGATAAAGTGTCTACCGATTCATTGATTATGATCTTTGAATCTGAAGGCGCAGCG
TCTGCTCCAGCAGCTGTCGAGGCTGCACCTGTTGCGGCTCCTGCTGCACCAGCTGCGGCAGCTGCGGCAGAGCTAAAAGA
AGTTCACGTCCCAGATATTGGTGACGACGAAGTTGAAGTGACTGAAATTTTAGTGACTGTTGGCGACAGCATCACTGAAG
AGCAATCGCTAATGAGCGTTGAAGGTGACAAAGCTTCTATGGAAGTACCTGCACCATTCGCTGGTGTGCTGAAAGAGATA
AAAGTGAACCTTGGTGACAAGGTTTCTACAGGTAGCCTAGTGATGGTATTTGAAGTAGCTGGTTCTGCAGCGGCTCAAGC
ACCTGCTGCGGCAGCCGTTGCTGCTCAAGTAGCATCTGCGCCATCAGTGCAAGATGTCAACGTACCAGATATTGGCGGTG
ATGAAGTTGAAGTGACTGAAATCATGGTTAAAGTTGGCGACACTGTTACCGAAGAGCAATCACTTATCAGCGTTGAAGGT
GATAAGGCTGCGATGGAAGTGCCTGCACCGTTTGCTGGTAAAGTGTTAGACATTAAAGTGGCTCAAGGCGATAAAGTATC
GACAGGCTCTTTGATTATGACTTTTGAAGTTGCAGGTAGCGCACCTGTTGCTGCACCTGCACAAGTTGCAGCGCCAACAG
TTGCTCCTACTGCGAGCCAAGCACCAGCAGCGAAAGAAGATTTTGTAGAGAATCATGCATACGCTCATGCTTCACCGGTT
ATCCGCCGTATGGCACGTGAACTAGGCGTTAACCTAGCTAACGTTAAGGGTACAGGTCGTAAGAACCGTATCATTAAAGA
AGATGTTCAAAACTACATCAAAGCGGTGATTAAGCAAGTTGAGTCAGGCGCAGTTAAAACAGCCGCTGCTGGTGGCGAGC
TAAACCTACTTGCATGGCCAAAAGTGGACTTCAGCAAGTTTGGTGAAACAGAAGTTAAACCGTTATCACGTATTCAGAAG
ATTTCTGGTGCTAATCTACACCGTAACTGGGTGAAGATCCCGCATGTTACCCAGTGGGATGACGCAGATATCACTGAACT
AGAAGCTTTCCGTAAGGCGCAAAATGCTGCCGAAGCGAAGAAAGACTCTGGCATGAAGATTACGCCGCTTGTGTTCATCA
TGAAGGCTGTTGCTAAAGCGCTAGAAGCGTTCCCAACCTTTAACTCTTCACTGTCAGAAGACGGCGAAAGCTTAATTTTG
AAGAAGTACGTTAACGTAGGTATTGCAGTAGATACACCAAACGGTTTAGTTGTTCCTGTGTTTAAAGACGTGAACAAGAA
AGGCATTCATGAGCTTTCTGATGAGCTGAAAGAGATCTCTAAGAAAGCCCGTGGTGGTAAGTTAACTGCTGCAGATATGC
AAGGCGGTTGCTTCACTATCTCTAGCTTAGGTGGCATCGGCGGTACGGCATTTACGCCAATCGTTAACGCACCAGAAGTG
GCTATTCTTGGTGTGTCTAAGTCAGACATGAAGCCTGTTTGGAATGGTAAAGAGTTTGAGCCGCGCTTAATGTTGCCGCT
ATCACTGTCTTATGATCATCGAGTGATTGATGGTGCCGATGGTGCGCGCTTTATCACGTACTTGAATAACTGCTTGTCAG
ACATTCGTACCTTAGTGCTATAA

Upstream 100 bases:

>100_bases
CGTGGCGATATTGAAAAGTCAGTGGTTGCAGCAGCAATTACTAAATTCAACATCGACGCTGACAAGACAAACCCGCTATA
CGCGTAAGAGGCAATACAAA

Downstream 100 bases:

>100_bases
ACAAATAGGCTGCTTGATTCAAGCAGCCTATTTTCTAGGTCGCTTATCTGAGTCGCTGACCTAATTCGCTGAATTGGCAC
TATTAGTAATCATCAGTAAG

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 620; Mature: 619

Protein sequence:

>620_residues
MTIEINVPDIGADEVEVTEILVSVGDKVEEEQSLISVEGDKAAMEVPASAAGIVKEIKVVVGDKVSTDSLIMIFESEGAA
SAPAAVEAAPVAAPAAPAAAAAAELKEVHVPDIGDDEVEVTEILVTVGDSITEEQSLMSVEGDKASMEVPAPFAGVLKEI
KVNLGDKVSTGSLVMVFEVAGSAAAQAPAAAAVAAQVASAPSVQDVNVPDIGGDEVEVTEIMVKVGDTVTEEQSLISVEG
DKAAMEVPAPFAGKVLDIKVAQGDKVSTGSLIMTFEVAGSAPVAAPAQVAAPTVAPTASQAPAAKEDFVENHAYAHASPV
IRRMARELGVNLANVKGTGRKNRIIKEDVQNYIKAVIKQVESGAVKTAAAGGELNLLAWPKVDFSKFGETEVKPLSRIQK
ISGANLHRNWVKIPHVTQWDDADITELEAFRKAQNAAEAKKDSGMKITPLVFIMKAVAKALEAFPTFNSSLSEDGESLIL
KKYVNVGIAVDTPNGLVVPVFKDVNKKGIHELSDELKEISKKARGGKLTAADMQGGCFTISSLGGIGGTAFTPIVNAPEV
AILGVSKSDMKPVWNGKEFEPRLMLPLSLSYDHRVIDGADGARFITYLNNCLSDIRTLVL

Sequences:

>Translated_620_residues
MTIEINVPDIGADEVEVTEILVSVGDKVEEEQSLISVEGDKAAMEVPASAAGIVKEIKVVVGDKVSTDSLIMIFESEGAA
SAPAAVEAAPVAAPAAPAAAAAAELKEVHVPDIGDDEVEVTEILVTVGDSITEEQSLMSVEGDKASMEVPAPFAGVLKEI
KVNLGDKVSTGSLVMVFEVAGSAAAQAPAAAAVAAQVASAPSVQDVNVPDIGGDEVEVTEIMVKVGDTVTEEQSLISVEG
DKAAMEVPAPFAGKVLDIKVAQGDKVSTGSLIMTFEVAGSAPVAAPAQVAAPTVAPTASQAPAAKEDFVENHAYAHASPV
IRRMARELGVNLANVKGTGRKNRIIKEDVQNYIKAVIKQVESGAVKTAAAGGELNLLAWPKVDFSKFGETEVKPLSRIQK
ISGANLHRNWVKIPHVTQWDDADITELEAFRKAQNAAEAKKDSGMKITPLVFIMKAVAKALEAFPTFNSSLSEDGESLIL
KKYVNVGIAVDTPNGLVVPVFKDVNKKGIHELSDELKEISKKARGGKLTAADMQGGCFTISSLGGIGGTAFTPIVNAPEV
AILGVSKSDMKPVWNGKEFEPRLMLPLSLSYDHRVIDGADGARFITYLNNCLSDIRTLVL
>Mature_619_residues
TIEINVPDIGADEVEVTEILVSVGDKVEEEQSLISVEGDKAAMEVPASAAGIVKEIKVVVGDKVSTDSLIMIFESEGAAS
APAAVEAAPVAAPAAPAAAAAAELKEVHVPDIGDDEVEVTEILVTVGDSITEEQSLMSVEGDKASMEVPAPFAGVLKEIK
VNLGDKVSTGSLVMVFEVAGSAAAQAPAAAAVAAQVASAPSVQDVNVPDIGGDEVEVTEIMVKVGDTVTEEQSLISVEGD
KAAMEVPAPFAGKVLDIKVAQGDKVSTGSLIMTFEVAGSAPVAAPAQVAAPTVAPTASQAPAAKEDFVENHAYAHASPVI
RRMARELGVNLANVKGTGRKNRIIKEDVQNYIKAVIKQVESGAVKTAAAGGELNLLAWPKVDFSKFGETEVKPLSRIQKI
SGANLHRNWVKIPHVTQWDDADITELEAFRKAQNAAEAKKDSGMKITPLVFIMKAVAKALEAFPTFNSSLSEDGESLILK
KYVNVGIAVDTPNGLVVPVFKDVNKKGIHELSDELKEISKKARGGKLTAADMQGGCFTISSLGGIGGTAFTPIVNAPEVA
ILGVSKSDMKPVWNGKEFEPRLMLPLSLSYDHRVIDGADGARFITYLNNCLSDIRTLVL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=436, Percent_Identity=30.5045871559633, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI31711992, Length=336, Percent_Identity=31.25, Blast_Score=139, Evalue=9e-33,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=32.15859030837, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI203098816, Length=447, Percent_Identity=27.7404921700224, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI203098753, Length=447, Percent_Identity=27.7404921700224, Blast_Score=113, Evalue=7e-25,
Organism=Homo sapiens, GI260898739, Length=155, Percent_Identity=36.1290322580645, Blast_Score=96, Evalue=8e-20,
Organism=Escherichia coli, GI1786305, Length=632, Percent_Identity=69.9367088607595, Blast_Score=785, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=397, Percent_Identity=30.2267002518892, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=423, Percent_Identity=29.5508274231678, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=496, Percent_Identity=29.4354838709677, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI25146366, Length=208, Percent_Identity=36.0576923076923, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17538894, Length=311, Percent_Identity=30.5466237942122, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320352, Length=413, Percent_Identity=27.3607748184019, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324258, Length=432, Percent_Identity=28.4722222222222, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI18859875, Length=425, Percent_Identity=31.5294117647059, Blast_Score=171, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24645909, Length=217, Percent_Identity=34.5622119815668, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=30.6034482758621, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI20129315, Length=232, Percent_Identity=30.6034482758621, Blast_Score=112, Evalue=9e-25,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 64854; Mature: 64722

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEINVPDIGADEVEVTEILVSVGDKVEEEQSLISVEGDKAAMEVPASAAGIVKEIKVV
CEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHEEEECCCCHHEECCCHHHHHHHHHHHH
VGDKVSTDSLIMIFESEGAASAPAAVEAAPVAAPAAPAAAAAAELKEVHVPDIGDDEVEV
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHH
TEILVTVGDSITEEQSLMSVEGDKASMEVPAPFAGVLKEIKVNLGDKVSTGSLVMVFEVA
HHHHHHHCCCHHHHHHHHEECCCCCCEECCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEC
GSAAAQAPAAAAVAAQVASAPSVQDVNVPDIGGDEVEVTEIMVKVGDTVTEEQSLISVEG
CCHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCCCCCCEEEECC
DKAAMEVPAPFAGKVLDIKVAQGDKVSTGSLIMTFEVAGSAPVAAPAQVAAPTVAPTASQ
CCCEEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCHHHCCCCCCCCCCC
APAAKEDFVENHAYAHASPVIRRMARELGVNLANVKGTGRKNRIIKEDVQNYIKAVIKQV
CCCCHHHHHHHCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
ESGAVKTAAAGGELNLLAWPKVDFSKFGETEVKPLSRIQKISGANLHRNWVKIPHVTQWD
HCCCEEEECCCCCEEEEECCCCCHHHCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCC
DADITELEAFRKAQNAAEAKKDSGMKITPLVFIMKAVAKALEAFPTFNSSLSEDGESLIL
CCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHH
KKYVNVGIAVDTPNGLVVPVFKDVNKKGIHELSDELKEISKKARGGKLTAADMQGGCFTI
HHHHCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEE
SSLGGIGGTAFTPIVNAPEVAILGVSKSDMKPVWNGKEFEPRLMLPLSLSYDHRVIDGAD
ECCCCCCCCHHCCCCCCCCEEEEECCHHCCCCCCCCCCCCCEEEEEEECCCCCEEEECCC
GARFITYLNNCLSDIRTLVL
CHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIEINVPDIGADEVEVTEILVSVGDKVEEEQSLISVEGDKAAMEVPASAAGIVKEIKVV
EEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHEEEECCCCHHEECCCHHHHHHHHHHHH
VGDKVSTDSLIMIFESEGAASAPAAVEAAPVAAPAAPAAAAAAELKEVHVPDIGDDEVEV
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHH
TEILVTVGDSITEEQSLMSVEGDKASMEVPAPFAGVLKEIKVNLGDKVSTGSLVMVFEVA
HHHHHHHCCCHHHHHHHHEECCCCCCEECCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEC
GSAAAQAPAAAAVAAQVASAPSVQDVNVPDIGGDEVEVTEIMVKVGDTVTEEQSLISVEG
CCHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHHHHCCCCCCCCCEEEECC
DKAAMEVPAPFAGKVLDIKVAQGDKVSTGSLIMTFEVAGSAPVAAPAQVAAPTVAPTASQ
CCCEEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCHHHCCCCCCCCCCC
APAAKEDFVENHAYAHASPVIRRMARELGVNLANVKGTGRKNRIIKEDVQNYIKAVIKQV
CCCCHHHHHHHCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
ESGAVKTAAAGGELNLLAWPKVDFSKFGETEVKPLSRIQKISGANLHRNWVKIPHVTQWD
HCCCEEEECCCCCEEEEECCCCCHHHCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCC
DADITELEAFRKAQNAAEAKKDSGMKITPLVFIMKAVAKALEAFPTFNSSLSEDGESLIL
CCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHH
KKYVNVGIAVDTPNGLVVPVFKDVNKKGIHELSDELKEISKKARGGKLTAADMQGGCFTI
HHHHCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEE
SSLGGIGGTAFTPIVNAPEVAILGVSKSDMKPVWNGKEFEPRLMLPLSLSYDHRVIDGAD
ECCCCCCCCHHCCCCCCCCEEEEECCHHCCCCCCCCCCCCCEEEEEEECCCCCEEEECCC
GARFITYLNNCLSDIRTLVL
CHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]