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The map label for this gene is lpdA [H]

Identifier: 157960250

GI number: 157960250

Start: 516799

End: 518226

Strand: Direct

Name: lpdA [H]

Synonym: Spea_0421

Alternate gene names: 157960250

Gene position: 516799-518226 (Clockwise)

Preceding gene: 157960249

Following gene: 157960251

Centisome position: 9.99

GC content: 45.31

Gene sequence:

>1428_bases
ATGAGTAACGAAATTAAAACTCAGGTAGTTGTACTAGGCGCTGGCCCTGCTGGCTACTCCGCTGCATTCCGTGCTGCAGA
TTTAGGTCTAGAGACTGTTATCGTTGAACGTTTCAGCACATTAGGCGGCGTATGTCTTAACGTCGGTTGTATCCCTTCTA
AAGCTTTGCTTCATGTTTCTAAAGTGATCGAAGAAGCTAAAGCGGTTTCGAACCACGGTGTTGTTTTTGGTGAGCCACAG
ATTGATCTTGATAAGCTACGTGAATACAAGCAATCGGTAATTGGCCAGTTAACTAACGGTTTAGGCGGCATGTCTAAGAT
GCGTAAAGTTAACGTAGTTAACGGTCTTGGTAAGTTCACAGGCCCTAACACGCTAGAAGTACAAGGTGAAGATGGCGTTA
AAGTTGTTCATTTTGAACAAGCGATTATTGCTGCGGGTTCGCGCCCAATTCAATTGCCATTTATTCCTCATGAAGATCCA
CGTGTATGGGATTCAACTGATGCGCTAGAGCTTAAAGAAGTTCCAGGCAAGCTACTTGTTATGGGCGGCGGCATCATCGG
CCTAGAAATGGGTACTGTGTACTCTTCACTAGGTAGTGAGATTGACGTAGTTGAGATGTTCGACCAAGTTATCCCTGCTG
CTGACAAAGACGTTGTTCGCGTTTACACCAAGAAGATCAAGAAGAAGTTCAACTTGATGCTTGAAACCAAGGTGACAGCC
GTTGAAGCCAAAGAAGATGGCATCTACGTGACGATGGAAGGCAAGAAAGCACCTGCTGAGCCTGTTCGTTATGACGCAGT
ATTGGTTGCTATCGGCCGTGTTCCAAACGGTAAAGGTCTAGACGCAGAGAAAGCAGGCGTTAATGTTGATGAGCGTGGCT
TTATCAATGTTGATAAGCAGATGCGTACTAACGTACCAAACATCTACGCTATCGGTGACATCGTTGGTCAACCAATGTTG
GCACACAAAGGTGTGCATGAAGGTCACGTTGCTGCAGAAGTGATTTCTGGTCTTAAGCATTACTTCGACCCTAAAGTTAT
CCCATCAATTGCGTACACAGACCCAGAAGTCGCTTGGGTTGGTCTAACTGAGAAAGAAGCTAAAGAGCAAGGTGTTGCTT
ACGAAACTGCGACTTTCCCATGGGCTGCAAGTGGCCGTGCAATTGCATCAGATTGTGCTGACGGCATGACTAAGCTTATT
TTCGACAAAGAGACTCACCGTGTAATTGGTGGTGCTATTGTTGGTGTTAATGGTGGCGAGCTTCTAGGTGAAATTGGCCT
AGCAATTGAAATGGGTTGTGATGCTGAAGATTTAGCGTTAACTATCCATGCTCACCCAACTCTACATGAGTCTGTAGGCC
TAGCGGCTGAGATTTATGAAGGTTCAATTACTGATTTGCCAAACCCAAAGGCAAAGAAGAAAAAGTAA

Upstream 100 bases:

>100_bases
AAATGCGGCCACCTTACAAGATTTGGCCACAGTTTATCGTTGAGTCGGTATTTATCGTTAAGTCGGTGCCACCAACGACA
TAAAAAGAATAGAGGAAAAC

Downstream 100 bases:

>100_bases
GCATTTACTAATGTTTTAAAAGAGCGCTCATTTGAGCGCTTTTTTTATATCTAATAACTCAGAATAGTCGGTTTTAAGTG
AACAATAGGTGAATCCATGT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLETVIVERFSTLGGVCLNVGCIPSKALLHVSKVIEEAKAVSNHGVVFGEPQ
IDLDKLREYKQSVIGQLTNGLGGMSKMRKVNVVNGLGKFTGPNTLEVQGEDGVKVVHFEQAIIAAGSRPIQLPFIPHEDP
RVWDSTDALELKEVPGKLLVMGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVYTKKIKKKFNLMLETKVTA
VEAKEDGIYVTMEGKKAPAEPVRYDAVLVAIGRVPNGKGLDAEKAGVNVDERGFINVDKQMRTNVPNIYAIGDIVGQPML
AHKGVHEGHVAAEVISGLKHYFDPKVIPSIAYTDPEVAWVGLTEKEAKEQGVAYETATFPWAASGRAIASDCADGMTKLI
FDKETHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKKK

Sequences:

>Translated_475_residues
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLETVIVERFSTLGGVCLNVGCIPSKALLHVSKVIEEAKAVSNHGVVFGEPQ
IDLDKLREYKQSVIGQLTNGLGGMSKMRKVNVVNGLGKFTGPNTLEVQGEDGVKVVHFEQAIIAAGSRPIQLPFIPHEDP
RVWDSTDALELKEVPGKLLVMGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVYTKKIKKKFNLMLETKVTA
VEAKEDGIYVTMEGKKAPAEPVRYDAVLVAIGRVPNGKGLDAEKAGVNVDERGFINVDKQMRTNVPNIYAIGDIVGQPML
AHKGVHEGHVAAEVISGLKHYFDPKVIPSIAYTDPEVAWVGLTEKEAKEQGVAYETATFPWAASGRAIASDCADGMTKLI
FDKETHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKKK
>Mature_474_residues
SNEIKTQVVVLGAGPAGYSAAFRAADLGLETVIVERFSTLGGVCLNVGCIPSKALLHVSKVIEEAKAVSNHGVVFGEPQI
DLDKLREYKQSVIGQLTNGLGGMSKMRKVNVVNGLGKFTGPNTLEVQGEDGVKVVHFEQAIIAAGSRPIQLPFIPHEDPR
VWDSTDALELKEVPGKLLVMGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVYTKKIKKKFNLMLETKVTAV
EAKEDGIYVTMEGKKAPAEPVRYDAVLVAIGRVPNGKGLDAEKAGVNVDERGFINVDKQMRTNVPNIYAIGDIVGQPMLA
HKGVHEGHVAAEVISGLKHYFDPKVIPSIAYTDPEVAWVGLTEKEAKEQGVAYETATFPWAASGRAIASDCADGMTKLIF
DKETHRVIGGAIVGVNGGELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=454, Percent_Identity=42.2907488986784, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI50301238, Length=453, Percent_Identity=27.8145695364238, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI33519430, Length=432, Percent_Identity=29.6296296296296, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI33519428, Length=432, Percent_Identity=29.6296296296296, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI33519426, Length=432, Percent_Identity=29.6296296296296, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI148277065, Length=432, Percent_Identity=29.6296296296296, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI148277071, Length=432, Percent_Identity=29.6296296296296, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI291045266, Length=451, Percent_Identity=29.0465631929047, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI22035672, Length=458, Percent_Identity=29.0393013100437, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI291045268, Length=445, Percent_Identity=27.6404494382022, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1786307, Length=474, Percent_Identity=86.7088607594937, Blast_Score=850, Evalue=0.0,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=28.5714285714286, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI87082354, Length=473, Percent_Identity=28.1183932346723, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1789915, Length=447, Percent_Identity=27.9642058165548, Blast_Score=144, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI32565766, Length=454, Percent_Identity=39.8678414096916, Blast_Score=328, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI17557007, Length=477, Percent_Identity=28.9308176100629, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71983429, Length=443, Percent_Identity=27.0880361173815, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI71983419, Length=443, Percent_Identity=27.0880361173815, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=448, Percent_Identity=25.8928571428571, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=40.1315789473684, Blast_Score=307, Evalue=3e-84,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=27.7185501066098, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6325166, Length=452, Percent_Identity=27.6548672566372, Blast_Score=153, Evalue=5e-38,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=41.1378555798687, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI24640553, Length=461, Percent_Identity=29.5010845986985, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24640549, Length=461, Percent_Identity=29.5010845986985, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24640551, Length=461, Percent_Identity=29.5010845986985, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17737741, Length=485, Percent_Identity=26.3917525773196, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50691; Mature: 50560

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLETVIVERFSTLGGVCLNVGCIPSKALLHVS
CCCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKAVSNHGVVFGEPQIDLDKLREYKQSVIGQLTNGLGGMSKMRKVNVVNGLGKFT
HHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHEEHHHHCCCCCC
GPNTLEVQGEDGVKVVHFEQAIIAAGSRPIQLPFIPHEDPRVWDSTDALELKEVPGKLLV
CCCEEEEECCCCEEEEEECHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEHHHCCCEEEE
MGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVYTKKIKKKFNLMLETKVTA
EECCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEEE
VEAKEDGIYVTMEGKKAPAEPVRYDAVLVAIGRVPNGKGLDAEKAGVNVDERGFINVDKQ
EEECCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHCCCCCCCCCCEEECHH
MRTNVPNIYAIGDIVGQPMLAHKGVHEGHVAAEVISGLKHYFDPKVIPSIAYTDPEVAWV
HHCCCCCEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEE
GLTEKEAKEQGVAYETATFPWAASGRAIASDCADGMTKLIFDKETHRVIGGAIVGVNGGE
ECCHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHEEECEEEEECCCHH
LLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKKK
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNEIKTQVVVLGAGPAGYSAAFRAADLGLETVIVERFSTLGGVCLNVGCIPSKALLHVS
CCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKAVSNHGVVFGEPQIDLDKLREYKQSVIGQLTNGLGGMSKMRKVNVVNGLGKFT
HHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHEEHHHHCCCCCC
GPNTLEVQGEDGVKVVHFEQAIIAAGSRPIQLPFIPHEDPRVWDSTDALELKEVPGKLLV
CCCEEEEECCCCEEEEEECHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEHHHCCCEEEE
MGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVYTKKIKKKFNLMLETKVTA
EECCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEEE
VEAKEDGIYVTMEGKKAPAEPVRYDAVLVAIGRVPNGKGLDAEKAGVNVDERGFINVDKQ
EEECCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHCCCCCCCCCCEEECHH
MRTNVPNIYAIGDIVGQPMLAHKGVHEGHVAAEVISGLKHYFDPKVIPSIAYTDPEVAWV
HHCCCCCEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEE
GLTEKEAKEQGVAYETATFPWAASGRAIASDCADGMTKLIFDKETHRVIGGAIVGVNGGE
ECCHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHEEECEEEEECCCHH
LLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKKK
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]