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The map label for this gene is ampE [H]

Identifier: 157960246

GI number: 157960246

Start: 509947

End: 510795

Strand: Direct

Name: ampE [H]

Synonym: Spea_0417

Alternate gene names: 157960246

Gene position: 509947-510795 (Clockwise)

Preceding gene: 157960245

Following gene: 157960247

Centisome position: 9.85

GC content: 43.58

Gene sequence:

>849_bases
ATGGCTTTGTTTGCGTTATTAGTCGCAATTTTAGTTGAGAGATTACGACTTTTGCCTAATTCTTGGCAGTTTGATGCCTT
GTTATCAAAATATAACAGGTCGCTTATCAGTGAACAGCAGCCATCATTTTTGATAATGACATTAGCGATTCTACTGCCCG
CAGTTCTTGTCTATATCTTGTCTTGGCTAGTGTCTGGCGTGTTATGGGGAGCTGTCAGTGTGGCGCTATGGGTGTCGATT
GCCGTGCTTTGCTTTAGCCATAATGTACAAAGAACGGCCTTCAAGCGTTATATACAAGCGGCTTGCCGTGGTGACTCACA
AGCCTGCTTTAATTATGCCAATGAGTTGGATTCTAGCCTCTGTTTAGAGTCAGTTAATGAGAAGGAACTGGGCGAAAAAG
TAGGCCAAAGTGTCGCGTGGATCAATTATCGCTATTACGGTGCGATTGCACTTTATCTTATTATCCTAGGGCCTGTAGGC
ACAGTGCTTTATTGCACGGTACGTTTTTATAGTGAACAGAGCCGTAAGCATGATTCTGCTCTGCCTTTAGTTGATAACCT
ACTATTCGTACTTGATTGGATCCCAAGTCGAGTATTTTCATTTGGTTACGTGTTAAGTGGCCATTTCAGACAAGCCATTT
CAGCCTGGTTGTCACATGCATTGAATCCTCAATCTTCGGCTCGTGACACGATTACTCAAGTCGCAGTTGCAGCAGAAACA
TTGCCCGAGTCATCATCGGCACCCATTTGTTTACAGTCGACTATCGCACTGCTGCAGTTGAGCAAGCGTAATTTTATCCT
ACTGATTACCGTACTTTCGTTGATGACAATATTTGGAGTGGTGAGTTAG

Upstream 100 bases:

>100_bases
CTTTGATTGGCAGCGTTATAAAGCTGCATTGTAAATAGAATGCGAGTTCGGCGGGAGCTAGGGTCGAAATGAGCAGATTA
ACCATTTAAAGGTGAAAATT

Downstream 100 bases:

>100_bases
TAGGCAGTCAGTCTGTTTTTGAAATAAAATAAAGGCTGTTTTTTAGCCCGTTACACAAGCGTGATTAGACCAAAAACCGT
ACGGAACATTCACAATGAAT

Product: regulatory protein AmpE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MALFALLVAILVERLRLLPNSWQFDALLSKYNRSLISEQQPSFLIMTLAILLPAVLVYILSWLVSGVLWGAVSVALWVSI
AVLCFSHNVQRTAFKRYIQAACRGDSQACFNYANELDSSLCLESVNEKELGEKVGQSVAWINYRYYGAIALYLIILGPVG
TVLYCTVRFYSEQSRKHDSALPLVDNLLFVLDWIPSRVFSFGYVLSGHFRQAISAWLSHALNPQSSARDTITQVAVAAET
LPESSSAPICLQSTIALLQLSKRNFILLITVLSLMTIFGVVS

Sequences:

>Translated_282_residues
MALFALLVAILVERLRLLPNSWQFDALLSKYNRSLISEQQPSFLIMTLAILLPAVLVYILSWLVSGVLWGAVSVALWVSI
AVLCFSHNVQRTAFKRYIQAACRGDSQACFNYANELDSSLCLESVNEKELGEKVGQSVAWINYRYYGAIALYLIILGPVG
TVLYCTVRFYSEQSRKHDSALPLVDNLLFVLDWIPSRVFSFGYVLSGHFRQAISAWLSHALNPQSSARDTITQVAVAAET
LPESSSAPICLQSTIALLQLSKRNFILLITVLSLMTIFGVVS
>Mature_281_residues
ALFALLVAILVERLRLLPNSWQFDALLSKYNRSLISEQQPSFLIMTLAILLPAVLVYILSWLVSGVLWGAVSVALWVSIA
VLCFSHNVQRTAFKRYIQAACRGDSQACFNYANELDSSLCLESVNEKELGEKVGQSVAWINYRYYGAIALYLIILGPVGT
VLYCTVRFYSEQSRKHDSALPLVDNLLFVLDWIPSRVFSFGYVLSGHFRQAISAWLSHALNPQSSARDTITQVAVAAETL
PESSSAPICLQSTIALLQLSKRNFILLITVLSLMTIFGVVS

Specific function: Putative signaling protein in beta-lactamase regulation. AmpE seems not to act as a direct sensor for beta-lactams [H]

COG id: COG3725

COG function: function code V; Membrane protein required for beta-lactamase induction

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786301, Length=290, Percent_Identity=27.2413793103448, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: NA

Molecular weight: Translated: 31332; Mature: 31201

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALFALLVAILVERLRLLPNSWQFDALLSKYNRSLISEQQPSFLIMTLAILLPAVLVYIL
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
SWLVSGVLWGAVSVALWVSIAVLCFSHNVQRTAFKRYIQAACRGDSQACFNYANELDSSL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
CLESVNEKELGEKVGQSVAWINYRYYGAIALYLIILGPVGTVLYCTVRFYSEQSRKHDSA
HHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LPLVDNLLFVLDWIPSRVFSFGYVLSGHFRQAISAWLSHALNPQSSARDTITQVAVAAET
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
LPESSSAPICLQSTIALLQLSKRNFILLITVLSLMTIFGVVS
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALFALLVAILVERLRLLPNSWQFDALLSKYNRSLISEQQPSFLIMTLAILLPAVLVYIL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
SWLVSGVLWGAVSVALWVSIAVLCFSHNVQRTAFKRYIQAACRGDSQACFNYANELDSSL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
CLESVNEKELGEKVGQSVAWINYRYYGAIALYLIILGPVGTVLYCTVRFYSEQSRKHDSA
HHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LPLVDNLLFVLDWIPSRVFSFGYVLSGHFRQAISAWLSHALNPQSSARDTITQVAVAAET
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
LPESSSAPICLQSTIALLQLSKRNFILLITVLSLMTIFGVVS
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503 [H]