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The map label for this gene is ampD [H]

Identifier: 157960245

GI number: 157960245

Start: 509338

End: 509880

Strand: Direct

Name: ampD [H]

Synonym: Spea_0416

Alternate gene names: 157960245

Gene position: 509338-509880 (Clockwise)

Preceding gene: 157960233

Following gene: 157960246

Centisome position: 9.84

GC content: 46.59

Gene sequence:

>543_bases
TTGTCGCTGACTTTAGTCGATGGTTGGGTGGCGCAGGCGAGATGCTGCCAGTCACCACATTTTAATGCTAGGCCGCTTCA
TGAAGTGAGCTTGCTGGTGATCCACAATATTAGCCTGCCAGCAGGCTGTTTCGGTCAGCCTTATATTGATCAGTTATTTC
AAGGTTGCCTCGATATTGAAGCCGATACTAGCTTTAAAGATTTACAAGGCTTAGAAGTGTCGGCGCACTTTCTAATAAGG
CGCGACGGTGAGTTGGTGCAGTATGTCTCTTGTGATGATAGAGCTTGGCATGCCGGGGTATCTGAGTTTGAGGGCCGTAG
CAATTGTAATGACTTTGCCCTTGGTATTGAGCTTGAAGGCACTGATGCTCTCGCTTATTCGGAGGAGCAGTATCTAAGGC
TTATTGAGTTAACTAGGAGCCTAATGGTTCATTATCCAGATATAACACTTGCAAGAATTACTGGGCATAGTGATATCGCA
CCGGGACGCAAAACCGATCCGGGTAATGGCTTTGATTGGCAGCGTTATAAAGCTGCATTGTAA

Upstream 100 bases:

>100_bases
ATCGCCGTATTTAGAGGGTGATAAATCTCAAAAGCGCGACTATATTCATAGGGTTAAAACTGTTAAAGTGAATAATAATA
TTTGCTTTAGGATCATTGTT

Downstream 100 bases:

>100_bases
ATAGAATGCGAGTTCGGCGGGAGCTAGGGTCGAAATGAGCAGATTAACCATTTAAAGGTGAAAATTATGGCTTTGTTTGC
GTTATTAGTCGCAATTTTAG

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MSLTLVDGWVAQARCCQSPHFNARPLHEVSLLVIHNISLPAGCFGQPYIDQLFQGCLDIEADTSFKDLQGLEVSAHFLIR
RDGELVQYVSCDDRAWHAGVSEFEGRSNCNDFALGIELEGTDALAYSEEQYLRLIELTRSLMVHYPDITLARITGHSDIA
PGRKTDPGNGFDWQRYKAAL

Sequences:

>Translated_180_residues
MSLTLVDGWVAQARCCQSPHFNARPLHEVSLLVIHNISLPAGCFGQPYIDQLFQGCLDIEADTSFKDLQGLEVSAHFLIR
RDGELVQYVSCDDRAWHAGVSEFEGRSNCNDFALGIELEGTDALAYSEEQYLRLIELTRSLMVHYPDITLARITGHSDIA
PGRKTDPGNGFDWQRYKAAL
>Mature_179_residues
SLTLVDGWVAQARCCQSPHFNARPLHEVSLLVIHNISLPAGCFGQPYIDQLFQGCLDIEADTSFKDLQGLEVSAHFLIRR
DGELVQYVSCDDRAWHAGVSEFEGRSNCNDFALGIELEGTDALAYSEEQYLRLIELTRSLMVHYPDITLARITGHSDIAP
GRKTDPGNGFDWQRYKAAL

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=176, Percent_Identity=57.3863636363636, Blast_Score=207, Evalue=4e-55,
Organism=Escherichia coli, GI1787092, Length=130, Percent_Identity=35.3846153846154, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20130; Mature: 19999

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTLVDGWVAQARCCQSPHFNARPLHEVSLLVIHNISLPAGCFGQPYIDQLFQGCLDIE
CEEEEECCHHHHHHHHCCCCCCCCCHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCC
ADTSFKDLQGLEVSAHFLIRRDGELVQYVSCDDRAWHAGVSEFEGRSNCNDFALGIELEG
CCCCHHHHCCCEEEEEEEEECCCCEEEEECCCCCCHHCCHHHCCCCCCCCEEEEEEEECC
TDALAYSEEQYLRLIELTRSLMVHYPDITLARITGHSDIAPGRKTDPGNGFDWQRYKAAL
CCCEEECHHHHHHHHHHHHHHHEECCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
SLTLVDGWVAQARCCQSPHFNARPLHEVSLLVIHNISLPAGCFGQPYIDQLFQGCLDIE
EEEEECCHHHHHHHHCCCCCCCCCHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCC
ADTSFKDLQGLEVSAHFLIRRDGELVQYVSCDDRAWHAGVSEFEGRSNCNDFALGIELEG
CCCCHHHHCCCEEEEEEEEECCCCEEEEECCCCCCHHCCHHHCCCCCCCCEEEEEEEECC
TDALAYSEEQYLRLIELTRSLMVHYPDITLARITGHSDIAPGRKTDPGNGFDWQRYKAAL
CCCEEECHHHHHHHHHHHHHHHEECCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503; 7959070 [H]