Click here to switch to the map view.

The map label for this gene is tapD [H]

Identifier: 157960240

GI number: 157960240

Start: 503007

End: 503918

Strand: Reverse

Name: tapD [H]

Synonym: Spea_0411

Alternate gene names: 157960240

Gene position: 503918-503007 (Counterclockwise)

Preceding gene: 157960241

Following gene: 157960239

Centisome position: 9.74

GC content: 45.61

Gene sequence:

>912_bases
ATGACTGAACTAATTTCTGTTTTTAGCCAGAATCCTTGGCTTTTTATCGCGTTAAGCTTTGTGTTTGCTGCTGTTATCGG
TAGCTTTTTAAACGTGGTGATCCACCGTATGCCGGTGATGATGAAACGTGAATGGCAGCAAGAGTGTAATCACTACCTGA
GTGAATACAAAAAGCAGGTGTTCGATAGTAATAAACAGCAACTTGAAGCGCCAATTGATGCGTTTCCTATAAAATACAAC
CTAGTCGTTCCCGGTTCTGCCTGCCCTAAATGTAAAACCAATATCAAGGCTATTCATAACCTGCCAATGCTTGGCTGGTT
GATGCTAAGAGGCAAGTGCGCAGCATGTAGTAACCCGATTTCGGCTCGCTACCCTATTGTCGAGTTTATCACTGCCGGCT
TAGTCGCTTTCTTAGCCTGGCATTTTGGCCCAACTTTAGAGTTTGCTCTAACCGCTTTACTGACTTTTTGTCTTATCGCC
TTAACAGGCATAGATCTAGACGAGATGCTATTACCCGACCAAATTACCTTGCCCCTGTTATGGCTGGGGCTGATCATTAA
CTTAGGCGGCACCTTTGCAACAACATCCGATGCCTTAATTGGCGCCGCAGCCGGTTACCTAAGTTTATGGTGCGTGTTCT
GGCTGTTTAAACTGCTTACTGGCAAGGATGGTATGGGCTATGGCGACTTTAAGTTACTAGCCGTATTTGGCGCCTGGTTC
GGCTGGCAAGTACTGCCTCTTGTTATCTTACTTTCATCGCTCGTAGGCGCAGTAGTGGGTATAGCACTTATTGTGTTCAA
GAAATTAAACCATGGCAACCCAATTCCATTTGGACCTTACATCGCTGCTGCAGGCTGGATAGCCATGATCTGGGGCGAGA
GCATTACCAACTGGTATCTGTCCACTTTATAA

Upstream 100 bases:

>100_bases
TGGTTGGTGGTCTAATTGTCGCCATGTACTTACCCATCTTCCAGATGGGTAAAGTTGTAGGCGGTTAATTCCAGTATAAA
ATTCAAATTTAAGAAAAATA

Downstream 100 bases:

>100_bases
TGGCTATGAGGTAAAAACTATGGCTGATTTTATAGTTGGGCTAACAGGCGGTATAGGAAGTGGCAAAACTACCGTCGCCA
ATCTCTTTGCTGAACTCGGC

Product: prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTELISVFSQNPWLFIALSFVFAAVIGSFLNVVIHRMPVMMKREWQQECNHYLSEYKKQVFDSNKQQLEAPIDAFPIKYN
LVVPGSACPKCKTNIKAIHNLPMLGWLMLRGKCAACSNPISARYPIVEFITAGLVAFLAWHFGPTLEFALTALLTFCLIA
LTGIDLDEMLLPDQITLPLLWLGLIINLGGTFATTSDALIGAAAGYLSLWCVFWLFKLLTGKDGMGYGDFKLLAVFGAWF
GWQVLPLVILLSSLVGAVVGIALIVFKKLNHGNPIPFGPYIAAAGWIAMIWGESITNWYLSTL

Sequences:

>Translated_303_residues
MTELISVFSQNPWLFIALSFVFAAVIGSFLNVVIHRMPVMMKREWQQECNHYLSEYKKQVFDSNKQQLEAPIDAFPIKYN
LVVPGSACPKCKTNIKAIHNLPMLGWLMLRGKCAACSNPISARYPIVEFITAGLVAFLAWHFGPTLEFALTALLTFCLIA
LTGIDLDEMLLPDQITLPLLWLGLIINLGGTFATTSDALIGAAAGYLSLWCVFWLFKLLTGKDGMGYGDFKLLAVFGAWF
GWQVLPLVILLSSLVGAVVGIALIVFKKLNHGNPIPFGPYIAAAGWIAMIWGESITNWYLSTL
>Mature_302_residues
TELISVFSQNPWLFIALSFVFAAVIGSFLNVVIHRMPVMMKREWQQECNHYLSEYKKQVFDSNKQQLEAPIDAFPIKYNL
VVPGSACPKCKTNIKAIHNLPMLGWLMLRGKCAACSNPISARYPIVEFITAGLVAFLAWHFGPTLEFALTALLTFCLIAL
TGIDLDEMLLPDQITLPLLWLGLIINLGGTFATTSDALIGAAAGYLSLWCVFWLFKLLTGKDGMGYGDFKLLAVFGAWFG
WQVLPLVILLSSLVGAVVGIALIVFKKLNHGNPIPFGPYIAAAGWIAMIWGESITNWYLSTL

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the tapA pilin precursor during membrane translocation [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=283, Percent_Identity=31.8021201413428, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1789732, Length=142, Percent_Identity=46.4788732394366, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 33497; Mature: 33366

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELISVFSQNPWLFIALSFVFAAVIGSFLNVVIHRMPVMMKREWQQECNHYLSEYKKQV
CHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDSNKQQLEAPIDAFPIKYNLVVPGSACPKCKTNIKAIHNLPMLGWLMLRGKCAACSNPI
HCCCHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHCCHHHCCCCC
SARYPIVEFITAGLVAFLAWHFGPTLEFALTALLTFCLIALTGIDLDEMLLPDQITLPLL
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHH
WLGLIINLGGTFATTSDALIGAAAGYLSLWCVFWLFKLLTGKDGMGYGDFKLLAVFGAWF
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
GWQVLPLVILLSSLVGAVVGIALIVFKKLNHGNPIPFGPYIAAAGWIAMIWGESITNWYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
STL
HCC
>Mature Secondary Structure 
TELISVFSQNPWLFIALSFVFAAVIGSFLNVVIHRMPVMMKREWQQECNHYLSEYKKQV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA