Click here to switch to the map view.

The map label for this gene is nudE [H]

Identifier: 157959986

GI number: 157959986

Start: 171198

End: 171752

Strand: Direct

Name: nudE [H]

Synonym: Spea_0156

Alternate gene names: 157959986

Gene position: 171198-171752 (Clockwise)

Preceding gene: 157959984

Following gene: 157959987

Centisome position: 3.31

GC content: 45.23

Gene sequence:

>555_bases
ATGACTAAAAAGAATGAGAAACCGGAGATTTTGCACACTGAAATAGTGGCAACCAGTCGGCTGTTTCAGATTGAGCAGGT
GCACTTGAGGTTTTCTAATCAAGTCGAGCGTCAGTATGAGCGAATGAAAGGTAATAATCGTGGCGCTGTCATGGTTGTGC
CGGTGCTCAATGGCACCGAGCTTTTATTGGGTAGAGAGTATGCTGCTGGTACTCACAGCTATGAGTTAGGATTCCCCAAG
GGCTTGATAGATCCTGGTGAAGAAGCCCATGAAGCGGCTAACCGAGAGTTACAAGAGGAAATTGGCTTTGGTGCCAGAAA
GCTAACTCATTTGATGGAATTGAGCTTAGCACCGGGTTATTTCGCCAGTAAGATGCAAATCTTTTTAGCTGAAGATCTAT
ATGAGAGTCGACTAGAAGGCGATGAACCTGAGCCGATCGATATGGTTACCTGGCCGTTGGTAACATGGCAAGAGCTGTTG
TCGGAGGAAGATTTTTCTGAGTCTCGCAGTGTAAGCGCTTTATTTTTAGCTCAGAAACATCTACAACTTAGATAG

Upstream 100 bases:

>100_bases
TCCATGGAAACATAGTTGAGCAATCCTAAAAAATTCATGTTGTAGATTGGAGCGAGAATAGTAAGATTATACTTGAATTG
GTAGTTGCACTGGGATGTGT

Downstream 100 bases:

>100_bases
TTTAAATTGGCACTCGTTATTGGTATTAGGCTGTTGGAGTCATTATGAAGCCAGAGAATTATGTAGATCAAGTCATTGAA
ATTGCTACGCTCGCGGGTAG

Product: ADP-ribose diphosphatase NudE

Products: AMP; D-Ribose 5- Phosphate. [C]

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MTKKNEKPEILHTEIVATSRLFQIEQVHLRFSNQVERQYERMKGNNRGAVMVVPVLNGTELLLGREYAAGTHSYELGFPK
GLIDPGEEAHEAANRELQEEIGFGARKLTHLMELSLAPGYFASKMQIFLAEDLYESRLEGDEPEPIDMVTWPLVTWQELL
SEEDFSESRSVSALFLAQKHLQLR

Sequences:

>Translated_184_residues
MTKKNEKPEILHTEIVATSRLFQIEQVHLRFSNQVERQYERMKGNNRGAVMVVPVLNGTELLLGREYAAGTHSYELGFPK
GLIDPGEEAHEAANRELQEEIGFGARKLTHLMELSLAPGYFASKMQIFLAEDLYESRLEGDEPEPIDMVTWPLVTWQELL
SEEDFSESRSVSALFLAQKHLQLR
>Mature_183_residues
TKKNEKPEILHTEIVATSRLFQIEQVHLRFSNQVERQYERMKGNNRGAVMVVPVLNGTELLLGREYAAGTHSYELGFPKG
LIDPGEEAHEAANRELQEEIGFGARKLTHLMELSLAPGYFASKMQIFLAEDLYESRLEGDEPEPIDMVTWPLVTWQELLS
EEDFSESRSVSALFLAQKHLQLR

Specific function: Active on adenosine(5')triphospho(5')adenosine (Ap3A), ADP-ribose, NADH, adenosine(5')diphospho(5')adenosine (Ap2A) [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=182, Percent_Identity=59.8901098901099, Blast_Score=220, Evalue=4e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 21030; Mature: 20899

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKNEKPEILHTEIVATSRLFQIEQVHLRFSNQVERQYERMKGNNRGAVMVVPVLNGTE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCE
LLLGREYAAGTHSYELGFPKGLIDPGEEAHEAANRELQEEIGFGARKLTHLMELSLAPGY
EEEECCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCH
FASKMQIFLAEDLYESRLEGDEPEPIDMVTWPLVTWQELLSEEDFSESRSVSALFLAQKH
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LQLR
HCCC
>Mature Secondary Structure 
TKKNEKPEILHTEIVATSRLFQIEQVHLRFSNQVERQYERMKGNNRGAVMVVPVLNGTE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCE
LLLGREYAAGTHSYELGFPKGLIDPGEEAHEAANRELQEEIGFGARKLTHLMELSLAPGY
EEEECCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCH
FASKMQIFLAEDLYESRLEGDEPEPIDMVTWPLVTWQELLSEEDFSESRSVSALFLAQKH
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LQLR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ADP-Ribose; H2O [C]

Specific reaction: ADP-Ribose + H2O = AMP + D-Ribose 5- Phosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9452430 [H]