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The map label for this gene is fmt [H]

Identifier: 157959860

GI number: 157959860

Start: 33614

End: 34579

Strand: Reverse

Name: fmt [H]

Synonym: Spea_0030

Alternate gene names: 157959860

Gene position: 34579-33614 (Counterclockwise)

Preceding gene: 157959861

Following gene: 157959859

Centisome position: 0.67

GC content: 49.07

Gene sequence:

>966_bases
TTGAAACCATTAAATGTCATTTTTGCAGGAACACCCGATTTTGCTGCACGCCATCTTCAAGCGCTAATCGACTCAGAGCA
CAATGTGATCGCAGTCTACACCCAGCCAGATAGACCTGCCGGCCGTGGTAAAAAACTGCAAGCGAGTCCAGTCAAGGCAT
TAGCGCTTGAAAATGACATTGCAGTTTTTCAGCCTAAATCCCTACGTGACGAAGACGCCCAAGCAGAACTTGCAGCATTA
AACGCCGATATCATGGTTGTGGTGGCTTATGGTCTTATCTTGCCAAAAGTGGTGCTAGACACCCCACGCCTAGGTTGTAT
CAATGTGCACGGTTCTATCTTACCACGCTGGCGTGGTGCAGCCCCGATCCAACGTGCATTGTGGGCCGGCGATACTGAAA
CTGGCGTGACCATCATGCAGATGGATATTGGTCTAGATACCGGTGACATGCTACTTAAGACTCTGCTTCCTATTGAAGAC
AATGACACCTCTTCAACTCTTTATGAAAAACTAGCAGAGCAAGGCCCGACGGCACTAGTAGAAGCACTAGCAGGCATTGC
TGAAGGCACCCTTCCAGCAGAGAAGCAAGACGAGAGCCTAGCTAACTACGCCGAAAAGCTTTCAAAAGAGGAGGCCCGTC
TAGATTGGTCTAAGCCAGCTTCAGCATTATGGCGTGAAATTCGTGCCTTTAACCCTTGGCCTGTTAGCCACTACGAACAT
GAAGGCAATACCATCAAGGTATGGCAAAGTTCTGTTAGTGATGAGACCAGTTCACAGCAACCCGGCACCATATTATCGGC
AGATAAAAGCGGCATCAGCATTGCGACAGGCGAAGGCGTATTAACCATCACCCAGATGCAGTTACCAGGTAAGAAACCGT
TAAGTGTTGCCGACATTCTAAACTCTAGAGCCGACTGGTTTACCCCAGGCACAGTGCTCGCTAGCACCGACAATCAAGAG
GCTTAA

Upstream 100 bases:

>100_bases
GGCAAGCTGTTTGTTGACTATCTATCGCCTCTAAAGCGTCAACGTATCAAGCAGAAACTCGAAAAAGCGGCTCGTCTCGA
AGCGAAACAAGGATAAATTT

Downstream 100 bases:

>100_bases
TCGCTAATGAACTTAAGAGCTTTGGCCGCTAAGGTCGTTTTTCAGGTGCTAGAAAAAGGTATCTCACTTTCTGTGGCGCT
ACCCGATCAGCAAAGACATC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKPLNVIFAGTPDFAARHLQALIDSEHNVIAVYTQPDRPAGRGKKLQASPVKALALENDIAVFQPKSLRDEDAQAELAAL
NADIMVVVAYGLILPKVVLDTPRLGCINVHGSILPRWRGAAPIQRALWAGDTETGVTIMQMDIGLDTGDMLLKTLLPIED
NDTSSTLYEKLAEQGPTALVEALAGIAEGTLPAEKQDESLANYAEKLSKEEARLDWSKPASALWREIRAFNPWPVSHYEH
EGNTIKVWQSSVSDETSSQQPGTILSADKSGISIATGEGVLTITQMQLPGKKPLSVADILNSRADWFTPGTVLASTDNQE
A

Sequences:

>Translated_321_residues
MKPLNVIFAGTPDFAARHLQALIDSEHNVIAVYTQPDRPAGRGKKLQASPVKALALENDIAVFQPKSLRDEDAQAELAAL
NADIMVVVAYGLILPKVVLDTPRLGCINVHGSILPRWRGAAPIQRALWAGDTETGVTIMQMDIGLDTGDMLLKTLLPIED
NDTSSTLYEKLAEQGPTALVEALAGIAEGTLPAEKQDESLANYAEKLSKEEARLDWSKPASALWREIRAFNPWPVSHYEH
EGNTIKVWQSSVSDETSSQQPGTILSADKSGISIATGEGVLTITQMQLPGKKPLSVADILNSRADWFTPGTVLASTDNQE
A
>Mature_321_residues
MKPLNVIFAGTPDFAARHLQALIDSEHNVIAVYTQPDRPAGRGKKLQASPVKALALENDIAVFQPKSLRDEDAQAELAAL
NADIMVVVAYGLILPKVVLDTPRLGCINVHGSILPRWRGAAPIQRALWAGDTETGVTIMQMDIGLDTGDMLLKTLLPIED
NDTSSTLYEKLAEQGPTALVEALAGIAEGTLPAEKQDESLANYAEKLSKEEARLDWSKPASALWREIRAFNPWPVSHYEH
EGNTIKVWQSSVSDETSSQQPGTILSADKSGISIATGEGVLTITQMQLPGKKPLSVADILNSRADWFTPGTVLASTDNQE
A

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI21614513, Length=214, Percent_Identity=35.0467289719626, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI238814322, Length=317, Percent_Identity=29.3375394321767, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI164663775, Length=318, Percent_Identity=28.3018867924528, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1789683, Length=310, Percent_Identity=65.1612903225806, Blast_Score=416, Evalue=1e-118,
Organism=Escherichia coli, GI1788589, Length=284, Percent_Identity=31.3380281690141, Blast_Score=132, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI133930964, Length=245, Percent_Identity=30.2040816326531, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319458, Length=266, Percent_Identity=25.5639097744361, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI45550868, Length=324, Percent_Identity=27.4691358024691, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI28571984, Length=244, Percent_Identity=29.5081967213115, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585660, Length=239, Percent_Identity=30.1255230125523, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 34668; Mature: 34668

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLNVIFAGTPDFAARHLQALIDSEHNVIAVYTQPDRPAGRGKKLQASPVKALALENDI
CCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCE
AVFQPKSLRDEDAQAELAALNADIMVVVAYGLILPKVVLDTPRLGCINVHGSILPRWRGA
EEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
APIQRALWAGDTETGVTIMQMDIGLDTGDMLLKTLLPIEDNDTSSTLYEKLAEQGPTALV
CHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHH
EALAGIAEGTLPAEKQDESLANYAEKLSKEEARLDWSKPASALWREIRAFNPWPVSHYEH
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEC
EGNTIKVWQSSVSDETSSQQPGTILSADKSGISIATGEGVLTITQMQLPGKKPLSVADIL
CCCEEEEEECCCCCCCCCCCCCEEEECCCCCEEEEECCCEEEEEEEECCCCCCCHHHHHH
NSRADWFTPGTVLASTDNQEA
CCCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MKPLNVIFAGTPDFAARHLQALIDSEHNVIAVYTQPDRPAGRGKKLQASPVKALALENDI
CCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCE
AVFQPKSLRDEDAQAELAALNADIMVVVAYGLILPKVVLDTPRLGCINVHGSILPRWRGA
EEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
APIQRALWAGDTETGVTIMQMDIGLDTGDMLLKTLLPIEDNDTSSTLYEKLAEQGPTALV
CHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHH
EALAGIAEGTLPAEKQDESLANYAEKLSKEEARLDWSKPASALWREIRAFNPWPVSHYEH
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEC
EGNTIKVWQSSVSDETSSQQPGTILSADKSGISIATGEGVLTITQMQLPGKKPLSVADIL
CCCEEEEEECCCCCCCCCCCCCEEEECCCCCEEEEECCCEEEEEEEECCCCCCCHHHHHH
NSRADWFTPGTVLASTDNQEA
CCCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA