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The map label for this gene is rsmB [H]

Identifier: 157959859

GI number: 157959859

Start: 32327

End: 33607

Strand: Reverse

Name: rsmB [H]

Synonym: Spea_0029

Alternate gene names: 157959859

Gene position: 33607-32327 (Counterclockwise)

Preceding gene: 157959860

Following gene: 157959858

Centisome position: 0.65

GC content: 48.63

Gene sequence:

>1281_bases
ATGAACTTAAGAGCTTTGGCCGCTAAGGTCGTTTTTCAGGTGCTAGAAAAAGGTATCTCACTTTCTGTGGCGCTACCCGA
TCAGCAAAGACATCTCGACAGTGGCAAAGACAAGGCGCTACTTGCCGAGCTTTGCTATGGCGTGATGCGTCATTTGCCAC
AACTAGACAAGCAAGTCAGCGATTGCATGAGCAAGCAGATGAAGGGTAAGCAGCGCATTGTGCACCAATTGCTGCTGATT
GGCTGCTACCAGCTGTACTTCACGCGTATTCCTAGCCACGCGGCAATATCTGAAACGGCTGAAGCCTGTCGTCAGCTAAA
GTTTGAAGGTTTAGTTAAGGTCGTTAATGGTGTCTTGCGTAATATTCAACGCCAAGAAAAACCATTAGCGACAGACAACG
ACACCTTAGCCTATAACACCCCAGCTTGGATCATTAAGCGTTTAAAGGATGCCTACCCTGATAACTGGCAAAGTGTTATT
GAGCAGAGTCACGAACGTCCGCCTATGTGGCTGCGTAACAATCAGCAATCGCAAACCAGAGAGACTTACCTACTAGCACT
GGCTGGATTAGAGATAGAAGCGCAGGCCGGTAACAGCCCAGACTCGATTTTATTATCTAGCCCACGTGATGTAATGCAAC
TACCAGGTTTTGAAACTGGCGCAGCCTCGGTGCAAGATGGCGCAGCTCAATGGGCAGCAACGCTACTGGCACCGCAGGGC
GACGAGCTAGTACTTGATGCTTGTGCGGCACCCGGCGGTAAAACTTGCCACCTGCTAGAACTTGCACCCAACATTAAGCT
GGTAGCGGTAGATTTCGACGCTAAACGCCTCGAACGTGTGCAGCAAAACCTTGATAGATTGTCACTCAAAGCTGAGTTAG
TGCATGGCGATGCCGCCAATATTGACTCATGGTGGCAAGGTGACAAATTTGACCGTATCTTGCTCGATGCGCCTTGTTCG
GCAACAGGTGTTATTCGTCGTCACCCAGATATTAAGTGGCTAAGAAAGAACAACGACATCGAAGAGTTGGCGCAATTACA
GTCACAAATTTTCGATCATTGCTGGAAGTGGTTAAAGCCCGGTGGCACACTCTTATTCGCAACATGTTCGATTTTGCCTC
AAGAAAATAAAGATCAAGTCAGCGCCTTCTTAGCCAGAACACCTGATGCGACCTTAGTGCCAATAGAAGAGCAAGCCAAC
CCAGACGATATCGGCTGGCAGATTGTTCCAGGACAAGACAACATGGACGGCTTTTATTACGCTCGCCTAGTGAAGGATTA
G

Upstream 100 bases:

>100_bases
GAAACCGTTAAGTGTTGCCGACATTCTAAACTCTAGAGCCGACTGGTTTACCCCAGGCACAGTGCTCGCTAGCACCGACA
ATCAAGAGGCTTAATCGCTA

Downstream 100 bases:

>100_bases
AACGGCAATGAAAATTATCATTTTGGGTGCAGGACAAGTTGGCGGCACATTAGCCGAAAACTTAGTTGGCGAAAACAATG
ACATCACCATTGTCGATAAT

Product: sun protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MNLRALAAKVVFQVLEKGISLSVALPDQQRHLDSGKDKALLAELCYGVMRHLPQLDKQVSDCMSKQMKGKQRIVHQLLLI
GCYQLYFTRIPSHAAISETAEACRQLKFEGLVKVVNGVLRNIQRQEKPLATDNDTLAYNTPAWIIKRLKDAYPDNWQSVI
EQSHERPPMWLRNNQQSQTRETYLLALAGLEIEAQAGNSPDSILLSSPRDVMQLPGFETGAASVQDGAAQWAATLLAPQG
DELVLDACAAPGGKTCHLLELAPNIKLVAVDFDAKRLERVQQNLDRLSLKAELVHGDAANIDSWWQGDKFDRILLDAPCS
ATGVIRRHPDIKWLRKNNDIEELAQLQSQIFDHCWKWLKPGGTLLFATCSILPQENKDQVSAFLARTPDATLVPIEEQAN
PDDIGWQIVPGQDNMDGFYYARLVKD

Sequences:

>Translated_426_residues
MNLRALAAKVVFQVLEKGISLSVALPDQQRHLDSGKDKALLAELCYGVMRHLPQLDKQVSDCMSKQMKGKQRIVHQLLLI
GCYQLYFTRIPSHAAISETAEACRQLKFEGLVKVVNGVLRNIQRQEKPLATDNDTLAYNTPAWIIKRLKDAYPDNWQSVI
EQSHERPPMWLRNNQQSQTRETYLLALAGLEIEAQAGNSPDSILLSSPRDVMQLPGFETGAASVQDGAAQWAATLLAPQG
DELVLDACAAPGGKTCHLLELAPNIKLVAVDFDAKRLERVQQNLDRLSLKAELVHGDAANIDSWWQGDKFDRILLDAPCS
ATGVIRRHPDIKWLRKNNDIEELAQLQSQIFDHCWKWLKPGGTLLFATCSILPQENKDQVSAFLARTPDATLVPIEEQAN
PDDIGWQIVPGQDNMDGFYYARLVKD
>Mature_426_residues
MNLRALAAKVVFQVLEKGISLSVALPDQQRHLDSGKDKALLAELCYGVMRHLPQLDKQVSDCMSKQMKGKQRIVHQLLLI
GCYQLYFTRIPSHAAISETAEACRQLKFEGLVKVVNGVLRNIQRQEKPLATDNDTLAYNTPAWIIKRLKDAYPDNWQSVI
EQSHERPPMWLRNNQQSQTRETYLLALAGLEIEAQAGNSPDSILLSSPRDVMQLPGFETGAASVQDGAAQWAATLLAPQG
DELVLDACAAPGGKTCHLLELAPNIKLVAVDFDAKRLERVQQNLDRLSLKAELVHGDAANIDSWWQGDKFDRILLDAPCS
ATGVIRRHPDIKWLRKNNDIEELAQLQSQIFDHCWKWLKPGGTLLFATCSILPQENKDQVSAFLARTPDATLVPIEEQAN
PDDIGWQIVPGQDNMDGFYYARLVKD

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=369, Percent_Identity=27.6422764227642, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI76150623, Length=369, Percent_Identity=27.6422764227642, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI32698918, Length=188, Percent_Identity=32.9787234042553, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI39995082, Length=191, Percent_Identity=30.8900523560209, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI301336155, Length=191, Percent_Identity=30.8900523560209, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI40316918, Length=182, Percent_Identity=30.2197802197802, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI270288816, Length=221, Percent_Identity=30.7692307692308, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI8922322, Length=221, Percent_Identity=30.7692307692308, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI270288818, Length=197, Percent_Identity=31.9796954314721, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI23199998, Length=221, Percent_Identity=30.7692307692308, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI11545785, Length=263, Percent_Identity=27.3764258555133, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI2367212, Length=428, Percent_Identity=53.0373831775701, Blast_Score=430, Evalue=1e-122,
Organism=Escherichia coli, GI87081985, Length=221, Percent_Identity=31.6742081447964, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17536757, Length=311, Percent_Identity=27.3311897106109, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI25143471, Length=181, Percent_Identity=34.8066298342541, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71998419, Length=171, Percent_Identity=32.7485380116959, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17509941, Length=165, Percent_Identity=27.8787878787879, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115534021, Length=171, Percent_Identity=32.7485380116959, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=231, Percent_Identity=30.3030303030303, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319447, Length=187, Percent_Identity=31.5508021390374, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI22024126, Length=318, Percent_Identity=27.6729559748428, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19920494, Length=206, Percent_Identity=29.6116504854369, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21355201, Length=177, Percent_Identity=31.638418079096, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24668781, Length=176, Percent_Identity=31.8181818181818, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21356513, Length=176, Percent_Identity=31.25, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 47643; Mature: 47643

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRALAAKVVFQVLEKGISLSVALPDQQRHLDSGKDKALLAELCYGVMRHLPQLDKQVS
CCHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DCMSKQMKGKQRIVHQLLLIGCYQLYFTRIPSHAAISETAEACRQLKFEGLVKVVNGVLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIQRQEKPLATDNDTLAYNTPAWIIKRLKDAYPDNWQSVIEQSHERPPMWLRNNQQSQTR
HHHHHHCCCCCCCCCEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHH
ETYLLALAGLEIEAQAGNSPDSILLSSPRDVMQLPGFETGAASVQDGAAQWAATLLAPQG
HHHHHEEECCEEEECCCCCCCCEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
DELVLDACAAPGGKTCHLLELAPNIKLVAVDFDAKRLERVQQNLDRLSLKAELVHGDAAN
CCEEEEECCCCCCCEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHEECCCCCC
IDSWWQGDKFDRILLDAPCSATGVIRRHPDIKWLRKNNDIEELAQLQSQIFDHCWKWLKP
CCCCCCCCCCCEEEEECCCCCCCHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCC
GGTLLFATCSILPQENKDQVSAFLARTPDATLVPIEEQANPDDIGWQIVPGQDNMDGFYY
CCEEEEEEEHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCEEE
ARLVKD
EEEECC
>Mature Secondary Structure
MNLRALAAKVVFQVLEKGISLSVALPDQQRHLDSGKDKALLAELCYGVMRHLPQLDKQVS
CCHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DCMSKQMKGKQRIVHQLLLIGCYQLYFTRIPSHAAISETAEACRQLKFEGLVKVVNGVLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIQRQEKPLATDNDTLAYNTPAWIIKRLKDAYPDNWQSVIEQSHERPPMWLRNNQQSQTR
HHHHHHCCCCCCCCCEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHH
ETYLLALAGLEIEAQAGNSPDSILLSSPRDVMQLPGFETGAASVQDGAAQWAATLLAPQG
HHHHHEEECCEEEECCCCCCCCEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
DELVLDACAAPGGKTCHLLELAPNIKLVAVDFDAKRLERVQQNLDRLSLKAELVHGDAAN
CCEEEEECCCCCCCEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHEECCCCCC
IDSWWQGDKFDRILLDAPCSATGVIRRHPDIKWLRKNNDIEELAQLQSQIFDHCWKWLKP
CCCCCCCCCCCEEEEECCCCCCCHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCC
GGTLLFATCSILPQENKDQVSAFLARTPDATLVPIEEQANPDDIGWQIVPGQDNMDGFYY
CCEEEEEEEHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCEEE
ARLVKD
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]