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The map label for this gene is def [H]

Identifier: 157959861

GI number: 157959861

Start: 34584

End: 35183

Strand: Reverse

Name: def [H]

Synonym: Spea_0031

Alternate gene names: 157959861

Gene position: 35183-34584 (Counterclockwise)

Preceding gene: 157959865

Following gene: 157959860

Centisome position: 0.68

GC content: 43.67

Gene sequence:

>600_bases
ATGCCGCTAACGACTAAAATTGAGCCATTAGCCAAAGTCAGTATAACTAACTGGCTCAAATTTGACAGACTTGTTAAGAG
TTTAGGTATGAGTTTATTAAAAGTTTTACGTTTTCCCGACGAAAGATTACGAACAGTTGCACAGCCGATCACAGAGTTTA
ACCCTGCCCTTCAAACACAAATCGACGATATGTTCGAAACTATGTATGAAGAGAAGGGAATTGGCCTAGCGGCAACACAG
GTTGATTATCACCATCAACTTATCGTTATGGACCTTCAAGATGAGGTTGAACGCCCTAAAGTGTTCATCAATCTTGAAAT
TACCGCCAAGAGTGGTGACTTCTGTAACGAAGAGGGGTGCCTATCTGTCCCAGGTGTTTACGCAAAAGTGGACCGCGCCG
AATTTGTCACCATCAAGGCGCTAGATCGTGATGGTAAAGAGTTCACCTTGGAAGCCGATGGCTTATTCGCTATCTGTTTA
CAGCATGAATTAGACCACCTATCGGGCAAGCTGTTTGTTGACTATCTATCGCCTCTAAAGCGTCAACGTATCAAGCAGAA
ACTCGAAAAAGCGGCTCGTCTCGAAGCGAAACAAGGATAA

Upstream 100 bases:

>100_bases
ACCAATTATAAGTGAAGCAAGTAATATTAACCGTTTCATCAGGGTACCCATGCTATTTGCTCTTTTATTAGAGTATATGT
AGAGCTTTTGCTGTTATTGA

Downstream 100 bases:

>100_bases
ATTTTTGAAACCATTAAATGTCATTTTTGCAGGAACACCCGATTTTGCTGCACGCCATCTTCAAGCGCTAATCGACTCAG
AGCACAATGTGATCGCAGTC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MPLTTKIEPLAKVSITNWLKFDRLVKSLGMSLLKVLRFPDERLRTVAQPITEFNPALQTQIDDMFETMYEEKGIGLAATQ
VDYHHQLIVMDLQDEVERPKVFINLEITAKSGDFCNEEGCLSVPGVYAKVDRAEFVTIKALDRDGKEFTLEADGLFAICL
QHELDHLSGKLFVDYLSPLKRQRIKQKLEKAARLEAKQG

Sequences:

>Translated_199_residues
MPLTTKIEPLAKVSITNWLKFDRLVKSLGMSLLKVLRFPDERLRTVAQPITEFNPALQTQIDDMFETMYEEKGIGLAATQ
VDYHHQLIVMDLQDEVERPKVFINLEITAKSGDFCNEEGCLSVPGVYAKVDRAEFVTIKALDRDGKEFTLEADGLFAICL
QHELDHLSGKLFVDYLSPLKRQRIKQKLEKAARLEAKQG
>Mature_198_residues
PLTTKIEPLAKVSITNWLKFDRLVKSLGMSLLKVLRFPDERLRTVAQPITEFNPALQTQIDDMFETMYEEKGIGLAATQV
DYHHQLIVMDLQDEVERPKVFINLEITAKSGDFCNEEGCLSVPGVYAKVDRAEFVTIKALDRDGKEFTLEADGLFAICLQ
HELDHLSGKLFVDYLSPLKRQRIKQKLEKAARLEAKQG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=29.9363057324841, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1789682, Length=168, Percent_Identity=62.5, Blast_Score=213, Evalue=5e-57,
Organism=Drosophila melanogaster, GI24645726, Length=140, Percent_Identity=29.2857142857143, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 22668; Mature: 22536

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTTKIEPLAKVSITNWLKFDRLVKSLGMSLLKVLRFPDERLRTVAQPITEFNPALQTQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHH
IDDMFETMYEEKGIGLAATQVDYHHQLIVMDLQDEVERPKVFINLEITAKSGDFCNEEGC
HHHHHHHHHHHCCCCEEEEEECCCCEEEEEECHHHCCCCEEEEEEEEEECCCCCCCCCCC
LSVPGVYAKVDRAEFVTIKALDRDGKEFTLEADGLFAICLQHELDHLSGKLFVDYLSPLK
CCCCCHHEECCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHH
RQRIKQKLEKAARLEAKQG
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PLTTKIEPLAKVSITNWLKFDRLVKSLGMSLLKVLRFPDERLRTVAQPITEFNPALQTQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHH
IDDMFETMYEEKGIGLAATQVDYHHQLIVMDLQDEVERPKVFINLEITAKSGDFCNEEGC
HHHHHHHHHHHCCCCEEEEEECCCCEEEEEECHHHCCCCEEEEEEEEEECCCCCCCCCCC
LSVPGVYAKVDRAEFVTIKALDRDGKEFTLEADGLFAICLQHELDHLSGKLFVDYLSPLK
CCCCCHHEECCCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHH
RQRIKQKLEKAARLEAKQG
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]