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The map label for this gene is dnaN [H]

Identifier: 157959832

GI number: 157959832

Start: 1628

End: 2728

Strand: Direct

Name: dnaN [H]

Synonym: Spea_0002

Alternate gene names: 157959832

Gene position: 1628-2728 (Clockwise)

Preceding gene: 157959831

Following gene: 157959833

Centisome position: 0.03

GC content: 45.5

Gene sequence:

>1101_bases
ATGAAATTCTCAATTGATAGGGACGCCTTACTAAAACCGCTGCAATTAGTCAGTGGTGCTGTAGAGAGGCGACATAACTT
GCCTATACTGGCAAACCTTTTGGTTGAAGTGAGTAATCACTCACTTAAGTTAACTGGAACCGATTTAGAAGTCGAGTTAG
TCGGCGAAGTTCAGCTCGAAGGTGAGATCCTTGAGGGGCGTACCACGGTCCCAGCGAAAAAATTTTTAGATATTGTTAAG
TCGCTACCTGATCAGGTAGAGCTTAAGATTGAGCAGCAAGAAAACCGTCTGTTGTTACGTTCGGGTCGTAGCCGTTTTAC
TTTGGCGACCTTGCCTGCTGAAGAGTATCCAAATGTCGATGCCTTTGAGGCTGAGATTGAGTTTACCTTGAAGCAGGGCA
CCATGAAGTCGTTGATCGACGCGACTCAGTTCTCTATGGCTAACCAGGACGTGCGTTATTACCTTAACGGACTGTTATTG
GAAACCGAAGGCAATGTGCTGCGTGCGATTGCGACTGACGGGCACCGTTTGGCATTGAGCCATCGAATGATCGAGGCGTC
ACTGCCTGAAAAGCAAGTGATTGTGCCCCGCAAGGGCGTATTAGAGCTTATGCGTTCGTTTGAAGCAGACGATCTCGACG
TAACGATTGCGATTGGTGACAATGCGATTAGAGCGACAACGGCAACAGCAGTGTTTACCAGTAAACTTGTTGATGGGCGT
TTTCCTGATTATCGTCGTGTACTGCCAAAGGGTGGCGATAAAGTTGTTATTGCGAGCCGATTACAGCTAAAACAAGCGTT
ACTGCGCGCTTCTATTCTGTCGAATGAGAAGTTCCGTGGTGTTCGTGTCCAGCTGGAAAACAATTTAATCAAGATCACCG
CTAATAACCCTGAACAGGAAGAAGCGGAAGAGATCTTAGATGTTGAATATGAAAATACACCATTAGAGATAGGTTTTAAC
GTCAGTTACTTACTCGACGTTCTCAATAATCTACCTTCAGATGATGTACGCATTACCTTGATAGACGGTAACTCAAGCGC
GCTTATCGACAACCACATAGAGCAAGATTCTATGTATGTTGTTATGCCTATGCGTTTGTAG

Upstream 100 bases:

>100_bases
CGTAAAATTGCCCAGTTGCGTGAAGAAAGTCATGACATTAAAGAAGATTATGCTAACTTGATAAGAACTTTATCTTCTTA
ATATCAGGGATATTGAATCA

Downstream 100 bases:

>100_bases
CCTCCGGCCCTTTTCTATCTCGACTTAAGGTTAACCAAGTAAAGGCGTTAAATGTACTTTATCTGGTTAACCTAAGTGAC
TCAGTAATAGAACACTCCCC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKFSIDRDALLKPLQLVSGAVERRHNLPILANLLVEVSNHSLKLTGTDLEVELVGEVQLEGEILEGRTTVPAKKFLDIVK
SLPDQVELKIEQQENRLLLRSGRSRFTLATLPAEEYPNVDAFEAEIEFTLKQGTMKSLIDATQFSMANQDVRYYLNGLLL
ETEGNVLRAIATDGHRLALSHRMIEASLPEKQVIVPRKGVLELMRSFEADDLDVTIAIGDNAIRATTATAVFTSKLVDGR
FPDYRRVLPKGGDKVVIASRLQLKQALLRASILSNEKFRGVRVQLENNLIKITANNPEQEEAEEILDVEYENTPLEIGFN
VSYLLDVLNNLPSDDVRITLIDGNSSALIDNHIEQDSMYVVMPMRL

Sequences:

>Translated_366_residues
MKFSIDRDALLKPLQLVSGAVERRHNLPILANLLVEVSNHSLKLTGTDLEVELVGEVQLEGEILEGRTTVPAKKFLDIVK
SLPDQVELKIEQQENRLLLRSGRSRFTLATLPAEEYPNVDAFEAEIEFTLKQGTMKSLIDATQFSMANQDVRYYLNGLLL
ETEGNVLRAIATDGHRLALSHRMIEASLPEKQVIVPRKGVLELMRSFEADDLDVTIAIGDNAIRATTATAVFTSKLVDGR
FPDYRRVLPKGGDKVVIASRLQLKQALLRASILSNEKFRGVRVQLENNLIKITANNPEQEEAEEILDVEYENTPLEIGFN
VSYLLDVLNNLPSDDVRITLIDGNSSALIDNHIEQDSMYVVMPMRL
>Mature_366_residues
MKFSIDRDALLKPLQLVSGAVERRHNLPILANLLVEVSNHSLKLTGTDLEVELVGEVQLEGEILEGRTTVPAKKFLDIVK
SLPDQVELKIEQQENRLLLRSGRSRFTLATLPAEEYPNVDAFEAEIEFTLKQGTMKSLIDATQFSMANQDVRYYLNGLLL
ETEGNVLRAIATDGHRLALSHRMIEASLPEKQVIVPRKGVLELMRSFEADDLDVTIAIGDNAIRATTATAVFTSKLVDGR
FPDYRRVLPKGGDKVVIASRLQLKQALLRASILSNEKFRGVRVQLENNLIKITANNPEQEEAEEILDVEYENTPLEIGFN
VSYLLDVLNNLPSDDVRITLIDGNSSALIDNHIEQDSMYVVMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=366, Percent_Identity=59.5628415300546, Blast_Score=436, Evalue=1e-123,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40985; Mature: 40985

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSIDRDALLKPLQLVSGAVERRHNLPILANLLVEVSNHSLKLTGTDLEVELVGEVQLE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCEEEEEEEEEEEE
GEILEGRTTVPAKKFLDIVKSLPDQVELKIEQQENRLLLRSGRSRFTLATLPAEEYPNVD
CEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCEEEEEECCHHHCCCCC
AFEAEIEFTLKQGTMKSLIDATQFSMANQDVRYYLNGLLLETEGNVLRAIATDGHRLALS
EEEEEEEEEECCCHHHHHHHHHHHHHCCCHHHEEECEEEEEECCCEEEEEECCCCEEEEH
HRMIEASLPEKQVIVPRKGVLELMRSFEADDLDVTIAIGDNAIRATTATAVFTSKLVDGR
HHHHHHCCCCCEEECCCHHHHHHHHHCCCCCEEEEEEECCCCEEHHHHHHHHHHHHHCCC
FPDYRRVLPKGGDKVVIASRLQLKQALLRASILSNEKFRGVRVQLENNLIKITANNPEQE
CCHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEECCCCHH
EAEEILDVEYENTPLEIGFNVSYLLDVLNNLPSDDVRITLIDGNSSALIDNHIEQDSMYV
HHHHHHCCEECCCCEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCCCCCCEEE
VMPMRL
EEEECC
>Mature Secondary Structure
MKFSIDRDALLKPLQLVSGAVERRHNLPILANLLVEVSNHSLKLTGTDLEVELVGEVQLE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCEEEEEEEEEEEE
GEILEGRTTVPAKKFLDIVKSLPDQVELKIEQQENRLLLRSGRSRFTLATLPAEEYPNVD
CEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCEEEEEECCHHHCCCCC
AFEAEIEFTLKQGTMKSLIDATQFSMANQDVRYYLNGLLLETEGNVLRAIATDGHRLALS
EEEEEEEEEECCCHHHHHHHHHHHHHCCCHHHEEECEEEEEECCCEEEEEECCCCEEEEH
HRMIEASLPEKQVIVPRKGVLELMRSFEADDLDVTIAIGDNAIRATTATAVFTSKLVDGR
HHHHHHCCCCCEEECCCHHHHHHHHHCCCCCEEEEEEECCCCEEHHHHHHHHHHHHHCCC
FPDYRRVLPKGGDKVVIASRLQLKQALLRASILSNEKFRGVRVQLENNLIKITANNPEQE
CCHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEECCCCHH
EAEEILDVEYENTPLEIGFNVSYLLDVLNNLPSDDVRITLIDGNSSALIDNHIEQDSMYV
HHHHHHCCEECCCCEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCCCCCCEEE
VMPMRL
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]