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The map label for this gene is recF

Identifier: 157959833

GI number: 157959833

Start: 2849

End: 3931

Strand: Direct

Name: recF

Synonym: Spea_0003

Alternate gene names: 157959833

Gene position: 2849-3931 (Clockwise)

Preceding gene: 157959832

Following gene: 157959834

Centisome position: 0.06

GC content: 43.03

Gene sequence:

>1083_bases
ATGAGTCTTAGCCGTCTCCATATCGAATCTTTTCGTAATATATCTTCTGCGCAACTGCAGCCGGGTGACGGGCTGAACTT
AATCTATGGCCACAATGGCAGTGGCAAAACCAGTATTCTTGAGGCTATTTACTTCCTCGGTATGGGCCGCTCCTTTCGAA
GCCATCTTTCCCAGCGTGTGATTAAGAATGACGATGATGCATTGACCCTGTTTGCCAATATGGAAAGCGGTGATGAGCAG
AGTAAGATAGGTTTACGTCGTTTTAGAAGCGGTGATATTGAAGTTAAAATCAATGGTGATAAAGTAAAGCGTTTATCGAC
GCTCGCTGAAACTTTACCGATCCAAGTGATCACACCTGAGAGTTTTTCTCTGCTGTTCGAAGGCCCTAAATCTAGGCGTC
AGTTTATTGATTGGGGAGCGTTTCATTCTGACCCACGTTTTTATGCTGCATGGGTCAATGTACGTCGAATTTTAAAGCAG
CGAAATCAGTTACTCAGAGATGGTTCGCCCTATAGTTCGATTCAATTTTGGGATAAAGAATTTATTCGTTATGCTGAACT
GGTAACCGACATAAGAAAGCAATATGTAGACTCGTTAAATGAGCTACTTAAGGGTATAATCGGAGAGTTTTTACCTCAGG
TTGATGTTAAGGTTTCTTTTACCCGAGGGTGGGATGCAAAAACCGAGTATGCACAGTTACTCGAGACGCAATACCCTAGA
GATTTAGCCACAGGCTATACCGTCAGTGGGCCGCATAAAGCTGATTTACGTTTGCGGGTAGGGACACTACCTGCACAAGA
TGCTTTATCTCGTGGCCAGCTGAAATTGTTAGTCTGTGCATTACGCATTGCACAGGGAAAGTTGCTAAAACAACAAATAG
ATAAAAAAAGTATTTATCTGGTGGACGATCTTCCATCGGAATTGGATGCAAAGCATAGAAAACTATTGTTACAGCAATTA
GCGGATACCGGGGCACAAGTTTTTGTCACGGCTATTGAGCCTGCAGCAATAGTAGATTCGTTAATCACGCCGCCAAGTAA
GATGTTCCATGTGGAACAAGGTCGTGTAACGGTAATTGAATAA

Upstream 100 bases:

>100_bases
CGACTTAAGGTTAACCAAGTAAAGGCGTTAAATGTACTTTATCTGGTTAACCTAAGTGACTCAGTAATAGAACACTCCCC
CGTTGTTATAAAGGTCATCA

Downstream 100 bases:

>100_bases
CCGACGAGAGAATAATATGTCAGAGAATAGTTATGATTCTTCGAGTATTAAGGTTCTAAAGGGCCTTGATGCGGTACGTA
AAAGACCAGGCATGTACATC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MSLSRLHIESFRNISSAQLQPGDGLNLIYGHNGSGKTSILEAIYFLGMGRSFRSHLSQRVIKNDDDALTLFANMESGDEQ
SKIGLRRFRSGDIEVKINGDKVKRLSTLAETLPIQVITPESFSLLFEGPKSRRQFIDWGAFHSDPRFYAAWVNVRRILKQ
RNQLLRDGSPYSSIQFWDKEFIRYAELVTDIRKQYVDSLNELLKGIIGEFLPQVDVKVSFTRGWDAKTEYAQLLETQYPR
DLATGYTVSGPHKADLRLRVGTLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKKSIYLVDDLPSELDAKHRKLLLQQL
ADTGAQVFVTAIEPAAIVDSLITPPSKMFHVEQGRVTVIE

Sequences:

>Translated_360_residues
MSLSRLHIESFRNISSAQLQPGDGLNLIYGHNGSGKTSILEAIYFLGMGRSFRSHLSQRVIKNDDDALTLFANMESGDEQ
SKIGLRRFRSGDIEVKINGDKVKRLSTLAETLPIQVITPESFSLLFEGPKSRRQFIDWGAFHSDPRFYAAWVNVRRILKQ
RNQLLRDGSPYSSIQFWDKEFIRYAELVTDIRKQYVDSLNELLKGIIGEFLPQVDVKVSFTRGWDAKTEYAQLLETQYPR
DLATGYTVSGPHKADLRLRVGTLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKKSIYLVDDLPSELDAKHRKLLLQQL
ADTGAQVFVTAIEPAAIVDSLITPPSKMFHVEQGRVTVIE
>Mature_359_residues
SLSRLHIESFRNISSAQLQPGDGLNLIYGHNGSGKTSILEAIYFLGMGRSFRSHLSQRVIKNDDDALTLFANMESGDEQS
KIGLRRFRSGDIEVKINGDKVKRLSTLAETLPIQVITPESFSLLFEGPKSRRQFIDWGAFHSDPRFYAAWVNVRRILKQR
NQLLRDGSPYSSIQFWDKEFIRYAELVTDIRKQYVDSLNELLKGIIGEFLPQVDVKVSFTRGWDAKTEYAQLLETQYPRD
LATGYTVSGPHKADLRLRVGTLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKKSIYLVDDLPSELDAKHRKLLLQQLA
DTGAQVFVTAIEPAAIVDSLITPPSKMFHVEQGRVTVIE

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=357, Percent_Identity=46.4985994397759, Blast_Score=342, Evalue=2e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_SHEPA (A8GYE5)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001499867.1
- ProteinModelPortal:   A8GYE5
- SMR:   A8GYE5
- STRING:   A8GYE5
- GeneID:   5660404
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_0003
- NMPDR:   fig|398579.3.peg.3
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   CLSK905538
- BioCyc:   SPEA398579:SPEA_0003-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 40660; Mature: 40529

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSRLHIESFRNISSAQLQPGDGLNLIYGHNGSGKTSILEAIYFLGMGRSFRSHLSQRV
CCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
IKNDDDALTLFANMESGDEQSKIGLRRFRSGDIEVKINGDKVKRLSTLAETLPIQVITPE
HCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCEEEECCC
SFSLLFEGPKSRRQFIDWGAFHSDPRFYAAWVNVRRILKQRNQLLRDGSPYSSIQFWDKE
CCEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
FIRYAELVTDIRKQYVDSLNELLKGIIGEFLPQVDVKVSFTRGWDAKTEYAQLLETQYPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCH
DLATGYTVSGPHKADLRLRVGTLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKKSIYL
HHHCCCCCCCCCCCCEEEEECCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
VDDLPSELDAKHRKLLLQQLADTGAQVFVTAIEPAAIVDSLITPPSKMFHVEQGRVTVIE
EECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCHHHEEECCCCEEEEC
>Mature Secondary Structure 
SLSRLHIESFRNISSAQLQPGDGLNLIYGHNGSGKTSILEAIYFLGMGRSFRSHLSQRV
CCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
IKNDDDALTLFANMESGDEQSKIGLRRFRSGDIEVKINGDKVKRLSTLAETLPIQVITPE
HCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCEEEECCC
SFSLLFEGPKSRRQFIDWGAFHSDPRFYAAWVNVRRILKQRNQLLRDGSPYSSIQFWDKE
CCEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
FIRYAELVTDIRKQYVDSLNELLKGIIGEFLPQVDVKVSFTRGWDAKTEYAQLLETQYPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCH
DLATGYTVSGPHKADLRLRVGTLPAQDALSRGQLKLLVCALRIAQGKLLKQQIDKKSIYL
HHHCCCCCCCCCCCCEEEEECCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
VDDLPSELDAKHRKLLLQQLADTGAQVFVTAIEPAAIVDSLITPPSKMFHVEQGRVTVIE
EECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCHHHEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA