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The map label for this gene is dnaA [H]

Identifier: 157959831

GI number: 157959831

Start: 223

End: 1608

Strand: Direct

Name: dnaA [H]

Synonym: Spea_0001

Alternate gene names: 157959831

Gene position: 223-1608 (Clockwise)

Preceding gene: NA

Following gene: 157959832

Centisome position: 0.0

GC content: 42.78

Gene sequence:

>1386_bases
GTGGCGGTTTCACTTTGGCAACAATGTATCGCAAGGCTGCAAGATGAGCTCTCTGCTCAGCAATTCAGTATGTGGATAAG
ACCGTTACAAGCAGAAATGGAAGGCGATACACTCGTCATCTATGCGCCTAATCGTTTTGTTTTGGATTGGGTTAGGGATA
AATACCTAAATATCATCAACCAATTTTTTACTGAACAAATGGGTAGCGATGCGCCAAGTTTGCGTTTCGATATCGGTAGT
CGTCCGTCGGCTAAACCAGTTGTTCAGGCTACAGCAGCAGTAAGAACTAGCAGACCAGTTACTCGAGAAGTGACTAAGCC
TTCTTTTAATACTCCGCACGCAGAGCCAATGGCTAATGCTAATCACCGTAGTAACATTAATCCGACTTACCAGTTTGATA
ACTTCGTTGAAGGTAAATCAAACCAATTGGGTAAAGCGGCTGCTTTGCAGGTGGCTGAAAATCCTGGTGGTGCGTATAAC
CCCTTGTTCCTTTATGGTGGCACAGGTTTAGGTAAAACTCACTTATTGCACGCTGTTGGTAATGGCATTATTAAAAATAA
CCCGAATGCAAAAGTAGTTTACATGCATTCAGAGCGTTTCGTGCAAGACATGGTTAAGGCGTTACAAAATAACGCGATTG
AAGAGTTTAAGCGCTACTACCGTAGTGTTGATGCATTGTTTATCGATGACATCCAATTCTTCGCCAACAAAGATAGATCT
CAAGAAGAGTTCTTCCATACCTTTAATGCGTTATTAGAAGGTAATCATCAGATCATTCTGACATCGGATAAATACCCGAA
AGAGATCGACGGTGTTGAAGATCGTCTTAAATCTCGTTTTGGTTGGGGTCTAACAGTTGCCATTGAGCCACCAGAATTAG
AAACCCGTGTTGCGATCTTAATGCGTAAGGCACAAGAGAGTGGCATTAACTTACCCGATGAAGTGGCATTCTTTATTGCT
AAGCGCTTACGTTCGAACGTGCGTGAGCTTGAAGGTGCACTGAACCGTGTTATTGCTAACGCTAACTTCACTGGCCGTCC
GATCACTATCGATTTTGTGCGTGAAGCACTACGAGACTTACTTGCTCTACAAGAGAAGTTGGTCACTATCGATAATATTC
AGAAGACTGTAGCGGAATACTACAAGATCAAGATGGCAGATATGTTATCTAAGCGTCGTTCACGTAGTGTGGCGCGTCCA
AGACAGATGGCAATGGCGTTATCGAAAGAGTTAACTAACCAGAGCTTGCCAGAGATTGGCGATGCGTTTGGTGGTCGTGA
TCACACAACTGTATTACATGCATGTCGTAAAATTGCCCAGTTGCGTGAAGAAAGTCATGACATTAAAGAAGATTATGCTA
ACTTGATAAGAACTTTATCTTCTTAA

Upstream 100 bases:

>100_bases
AGTTGATCTATCTGGGGATAAATATATTTAAAGGATAGCGATCAGCGTGATCTCACTATAGAATAGACCTCTTTTGTATG
ATCTTCTGGGGATAACTACA

Downstream 100 bases:

>100_bases
TATCAGGGATATTGAATCAATGAAATTCTCAATTGATAGGGACGCCTTACTAAAACCGCTGCAATTAGTCAGTGGTGCTG
TAGAGAGGCGACATAACTTG

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MAVSLWQQCIARLQDELSAQQFSMWIRPLQAEMEGDTLVIYAPNRFVLDWVRDKYLNIINQFFTEQMGSDAPSLRFDIGS
RPSAKPVVQATAAVRTSRPVTREVTKPSFNTPHAEPMANANHRSNINPTYQFDNFVEGKSNQLGKAAALQVAENPGGAYN
PLFLYGGTGLGKTHLLHAVGNGIIKNNPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALFIDDIQFFANKDRS
QEEFFHTFNALLEGNHQIILTSDKYPKEIDGVEDRLKSRFGWGLTVAIEPPELETRVAILMRKAQESGINLPDEVAFFIA
KRLRSNVRELEGALNRVIANANFTGRPITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKMADMLSKRRSRSVARP
RQMAMALSKELTNQSLPEIGDAFGGRDHTTVLHACRKIAQLREESHDIKEDYANLIRTLSS

Sequences:

>Translated_461_residues
MAVSLWQQCIARLQDELSAQQFSMWIRPLQAEMEGDTLVIYAPNRFVLDWVRDKYLNIINQFFTEQMGSDAPSLRFDIGS
RPSAKPVVQATAAVRTSRPVTREVTKPSFNTPHAEPMANANHRSNINPTYQFDNFVEGKSNQLGKAAALQVAENPGGAYN
PLFLYGGTGLGKTHLLHAVGNGIIKNNPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALFIDDIQFFANKDRS
QEEFFHTFNALLEGNHQIILTSDKYPKEIDGVEDRLKSRFGWGLTVAIEPPELETRVAILMRKAQESGINLPDEVAFFIA
KRLRSNVRELEGALNRVIANANFTGRPITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKMADMLSKRRSRSVARP
RQMAMALSKELTNQSLPEIGDAFGGRDHTTVLHACRKIAQLREESHDIKEDYANLIRTLSS
>Mature_460_residues
AVSLWQQCIARLQDELSAQQFSMWIRPLQAEMEGDTLVIYAPNRFVLDWVRDKYLNIINQFFTEQMGSDAPSLRFDIGSR
PSAKPVVQATAAVRTSRPVTREVTKPSFNTPHAEPMANANHRSNINPTYQFDNFVEGKSNQLGKAAALQVAENPGGAYNP
LFLYGGTGLGKTHLLHAVGNGIIKNNPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALFIDDIQFFANKDRSQ
EEFFHTFNALLEGNHQIILTSDKYPKEIDGVEDRLKSRFGWGLTVAIEPPELETRVAILMRKAQESGINLPDEVAFFIAK
RLRSNVRELEGALNRVIANANFTGRPITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKMADMLSKRRSRSVARPR
QMAMALSKELTNQSLPEIGDAFGGRDHTTVLHACRKIAQLREESHDIKEDYANLIRTLSS

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=468, Percent_Identity=79.2735042735043, Blast_Score=762, Evalue=0.0,
Organism=Escherichia coli, GI226510964, Length=231, Percent_Identity=26.8398268398268, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 52233; Mature: 52102

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSLWQQCIARLQDELSAQQFSMWIRPLQAEMEGDTLVIYAPNRFVLDWVRDKYLNIIN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHH
QFFTEQMGSDAPSLRFDIGSRPSAKPVVQATAAVRTSRPVTREVTKPSFNTPHAEPMANA
HHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCC
NHRSNINPTYQFDNFVEGKSNQLGKAAALQVAENPGGAYNPLFLYGGTGLGKTHLLHAVG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEECCCCCCCCCEEEECCCCCCHHHHHHHHC
NGIIKNNPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALFIDDIQFFANKDRS
CCCEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QEEFFHTFNALLEGNHQIILTSDKYPKEIDGVEDRLKSRFGWGLTVAIEPPELETRVAIL
HHHHHHHHHHHHCCCCEEEEECCCCCCHHCCHHHHHHHHCCCCEEEEECCCCHHHHHHHH
MRKAQESGINLPDEVAFFIAKRLRSNVRELEGALNRVIANANFTGRPITIDFVREALRDL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHH
LALQEKLVTIDNIQKTVAEYYKIKMADMLSKRRSRSVARPRQMAMALSKELTNQSLPEIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
DAFGGRDHTTVLHACRKIAQLREESHDIKEDYANLIRTLSS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVSLWQQCIARLQDELSAQQFSMWIRPLQAEMEGDTLVIYAPNRFVLDWVRDKYLNIIN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHH
QFFTEQMGSDAPSLRFDIGSRPSAKPVVQATAAVRTSRPVTREVTKPSFNTPHAEPMANA
HHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCC
NHRSNINPTYQFDNFVEGKSNQLGKAAALQVAENPGGAYNPLFLYGGTGLGKTHLLHAVG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEECCCCCCCCCEEEECCCCCCHHHHHHHHC
NGIIKNNPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALFIDDIQFFANKDRS
CCCEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QEEFFHTFNALLEGNHQIILTSDKYPKEIDGVEDRLKSRFGWGLTVAIEPPELETRVAIL
HHHHHHHHHHHHCCCCEEEEECCCCCCHHCCHHHHHHHHCCCCEEEEECCCCHHHHHHHH
MRKAQESGINLPDEVAFFIAKRLRSNVRELEGALNRVIANANFTGRPITIDFVREALRDL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHH
LALQEKLVTIDNIQKTVAEYYKIKMADMLSKRRSRSVARPRQMAMALSKELTNQSLPEIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
DAFGGRDHTTVLHACRKIAQLREESHDIKEDYANLIRTLSS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA