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The map label for this gene is nupC [H]

Identifier: 157373093

GI number: 157373093

Start: 5383318

End: 5384502

Strand: Direct

Name: nupC [H]

Synonym: Spro_4861

Alternate gene names: 157373093

Gene position: 5383318-5384502 (Clockwise)

Preceding gene: 157373088

Following gene: 157373095

Centisome position: 98.8

GC content: 52.49

Gene sequence:

>1185_bases
ATGCCGCAAATTCTGCAATTTATTTTTGCCCTGGTGGTGATCATCGCCCTGGCGCTGCTGGTCAGCAACGACCGCAAAAA
AATCCGCCTGCGTTATATTATTCAACTTTTGGTCATTGAAGGCGCACTCGCCTATTTCTTCCTGCATTCAGAAAGTGGCC
TTAGCGTCATCAGACACGTCGCCGGCTTCTTTGAAACGCTGCTGACATACGCTGCGCAAGGCTCCGACTTTGTGTTTGGC
GGCATGAGCAAAGACGGGTTGGCGTTCATCTTCCTTGGGGTTTTGTGCCCGATTATCTTTATTTCTGCGCTAATCGGTAT
TTTGCAGCATTTCCGCATTCTGCCGCTGGTTATCCGTGTGGTCGGTACCCTGCTTTCCAAAGTTAACGGCATGGGCAAGC
TGGAGTCGTTCAACGCCGTCAGCACGCTGGTGCTCGGACAATCGGAAAACTTCATCGCCTACAAGGGCATCATTGGTGAT
ATCTCTCCGCGCCGCATGTACACCATGGCGGCAACCGCCATGTCGACAGTCTCCATGTCCATCGTTGGCGCCTATATGTC
GATGATCGATGCGCAATACGTAGTCGCTGCGTTGATCCTGAATATGTTCAGTACCTTTATCATTTTGTCGATCATCAACC
CCTATCAGTTGGACGATGAACCTGAGCTGAAGCTGAGCAAGCTGCATGAAGATCAGAGCTTCTTTGAAATGCTGGGCGAG
TACATTTTGGCGGGCTTCAAAATTGCGATGATTATCGCTGCCATGCTGATCGGCTTTATTGCACTGATTTCTGCGGTAAA
CGCCCTGTTCTCCGCCGTGTTCGGTATCAGCTTCCAGCAGATCCTGGGCTACGTATTCTATCCGTTGGCCTGGTTAATCG
GCATCCCGGCACCGGATGCGCTGCGAGCCGGCAGCATCATGGCGACCAAGCTGGTAGCCAATGAATTTGTGGCAATGATT
GAATTGAAGAAAATTGCGGCGGAGCTAACACCACGCGGCCTGGGCATCCTGTCGGTATTCCTGGTGTCCTTCGCCAACTT
TGCCTCTATCGGCATCGTGGCCGGGGCAATCAAAGGGCTTAACGAACGACAGGGCAACGTGGTTTCTCGTTTTGGCCTGA
AGCTGGTCTACGGCTCAACGCTGGTTAGCCTGCTGTCGGCGGCAATCGCCGGGCTGGTGCTATAA

Upstream 100 bases:

>100_bases
CTGATAGTATTTTATTCCAATTATCACCTAAGGTAATGAAAGGTGGGCCAAGCTGTGGCTAAATGTGTTTTTTCATGCCC
GCAAGCCGTACGGACCCTTT

Downstream 100 bases:

>100_bases
GCCAAACTCATATCCCGGCCTGCGGGCCGGGACTTCCTCAGAACGCGACACAGACAGGGGTATCAACCCGCATCACAGAA
TCCTGGGCAAACTGTTGCTT

Product: nucleoside transporter

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: Nucleoside-transport system protein nupC [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MPQILQFIFALVVIIALALLVSNDRKKIRLRYIIQLLVIEGALAYFFLHSESGLSVIRHVAGFFETLLTYAAQGSDFVFG
GMSKDGLAFIFLGVLCPIIFISALIGILQHFRILPLVIRVVGTLLSKVNGMGKLESFNAVSTLVLGQSENFIAYKGIIGD
ISPRRMYTMAATAMSTVSMSIVGAYMSMIDAQYVVAALILNMFSTFIILSIINPYQLDDEPELKLSKLHEDQSFFEMLGE
YILAGFKIAMIIAAMLIGFIALISAVNALFSAVFGISFQQILGYVFYPLAWLIGIPAPDALRAGSIMATKLVANEFVAMI
ELKKIAAELTPRGLGILSVFLVSFANFASIGIVAGAIKGLNERQGNVVSRFGLKLVYGSTLVSLLSAAIAGLVL

Sequences:

>Translated_394_residues
MPQILQFIFALVVIIALALLVSNDRKKIRLRYIIQLLVIEGALAYFFLHSESGLSVIRHVAGFFETLLTYAAQGSDFVFG
GMSKDGLAFIFLGVLCPIIFISALIGILQHFRILPLVIRVVGTLLSKVNGMGKLESFNAVSTLVLGQSENFIAYKGIIGD
ISPRRMYTMAATAMSTVSMSIVGAYMSMIDAQYVVAALILNMFSTFIILSIINPYQLDDEPELKLSKLHEDQSFFEMLGE
YILAGFKIAMIIAAMLIGFIALISAVNALFSAVFGISFQQILGYVFYPLAWLIGIPAPDALRAGSIMATKLVANEFVAMI
ELKKIAAELTPRGLGILSVFLVSFANFASIGIVAGAIKGLNERQGNVVSRFGLKLVYGSTLVSLLSAAIAGLVL
>Mature_393_residues
PQILQFIFALVVIIALALLVSNDRKKIRLRYIIQLLVIEGALAYFFLHSESGLSVIRHVAGFFETLLTYAAQGSDFVFGG
MSKDGLAFIFLGVLCPIIFISALIGILQHFRILPLVIRVVGTLLSKVNGMGKLESFNAVSTLVLGQSENFIAYKGIIGDI
SPRRMYTMAATAMSTVSMSIVGAYMSMIDAQYVVAALILNMFSTFIILSIINPYQLDDEPELKLSKLHEDQSFFEMLGEY
ILAGFKIAMIIAAMLIGFIALISAVNALFSAVFGISFQQILGYVFYPLAWLIGIPAPDALRAGSIMATKLVANEFVAMIE
LKKIAAELTPRGLGILSVFLVSFANFASIGIVAGAIKGLNERQGNVVSRFGLKLVYGSTLVSLLSAAIAGLVL

Specific function: Transports nucleosides with a high affinity except guanosine and deoxyguanosine. Driven by a proton motive force [H]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI42542381, Length=419, Percent_Identity=25.0596658711217, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI11545853, Length=411, Percent_Identity=23.1143552311436, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI227116277, Length=400, Percent_Identity=22.75, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1788737, Length=400, Percent_Identity=71, Blast_Score=572, Evalue=1e-164,
Organism=Escherichia coli, GI1788485, Length=409, Percent_Identity=30.0733496332518, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1788488, Length=409, Percent_Identity=30.0733496332518, Blast_Score=168, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17560276, Length=394, Percent_Identity=24.3654822335025, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71991794, Length=403, Percent_Identity=23.8213399503722, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI45552519, Length=407, Percent_Identity=25.7985257985258, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI45552517, Length=407, Percent_Identity=25.7985257985258, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI19921868, Length=407, Percent_Identity=25.7985257985258, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI281360430, Length=354, Percent_Identity=24.0112994350282, Blast_Score=98, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 42636; Mature: 42505

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQILQFIFALVVIIALALLVSNDRKKIRLRYIIQLLVIEGALAYFFLHSESGLSVIRHV
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AGFFETLLTYAAQGSDFVFGGMSKDGLAFIFLGVLCPIIFISALIGILQHFRILPLVIRV
HHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGTLLSKVNGMGKLESFNAVSTLVLGQSENFIAYKGIIGDISPRRMYTMAATAMSTVSMS
HHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
IVGAYMSMIDAQYVVAALILNMFSTFIILSIINPYQLDDEPELKLSKLHEDQSFFEMLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHH
YILAGFKIAMIIAAMLIGFIALISAVNALFSAVFGISFQQILGYVFYPLAWLIGIPAPDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHH
LRAGSIMATKLVANEFVAMIELKKIAAELTPRGLGILSVFLVSFANFASIGIVAGAIKGL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
NERQGNVVSRFGLKLVYGSTLVSLLSAAIAGLVL
CHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PQILQFIFALVVIIALALLVSNDRKKIRLRYIIQLLVIEGALAYFFLHSESGLSVIRHV
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AGFFETLLTYAAQGSDFVFGGMSKDGLAFIFLGVLCPIIFISALIGILQHFRILPLVIRV
HHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGTLLSKVNGMGKLESFNAVSTLVLGQSENFIAYKGIIGDISPRRMYTMAATAMSTVSMS
HHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
IVGAYMSMIDAQYVVAALILNMFSTFIILSIINPYQLDDEPELKLSKLHEDQSFFEMLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHH
YILAGFKIAMIIAAMLIGFIALISAVNALFSAVFGISFQQILGYVFYPLAWLIGIPAPDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHH
LRAGSIMATKLVANEFVAMIELKKIAAELTPRGLGILSVFLVSFANFASIGIVAGAIKGL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
NERQGNVVSRFGLKLVYGSTLVSLLSAAIAGLVL
CHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]