BacMap

Definition	Serratia proteamaculans 568 chromosome, complete genome.
Accession	NC_009832
Length	5,448,853

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The map label for this gene is hdhA [C]

Identifier: 157372193

GI number: 157372193

Start: 4388416

End: 4389210

Strand: Reverse

Name: hdhA [C]

Synonym: Spro_3959

Alternate gene names: 157372193

Gene position: 4389210-4388416 (Counterclockwise)

Preceding gene: 157372194

Following gene: 157372192

Centisome position: 80.55

GC content: 61.51

Gene sequence:

>795_bases
ATGAATTTTCAGCTTGAGCAACGGGTCGCAGTGGTCACCGGCGGTTCGTCGGGCATCGGTTTTGAGACCCTGCGCCTGCT
GTTGGCGGAAGGGGCGAAGGTGGCCTTTTGCGGCCGTAACCCGGACAAACTGGCCGGGGCCGAAGCCAGCCTGCGTAATG
AGTTTCCACAGGCGGAGATCCTGGCGCTGTGCTGCGACGTGTTGGACGAGGCGCAGGTGACGCAGTTTGCCGCTCAGGTG
ACGGCGCACTTCGGCGGGGTGGATCTGTTGATCAACAACGCTGGCCAGGGATTCGTCGCGCATTTTGACCAGACGCCACG
TAGCGCCTGGCTGCATGAGGCGGAGCTAAAACTGTTCGGCGTGATTAACCCGGTGCAGGCGTTTTTGCCGGCGCTGGAGC
GTTCGGATATCGCTTCAATTACCTGCGTGAACTCACTGCTGGCATTGCAGCCGGAAGAACACATGATCGCCACCTCGGCG
GCGCGCGCCGCACTGCTCAACATGACGCTGACGCTGTCGAAAGAGCTGGTGGACAAGGGCATTCGCGTCAACTCGATCCT
GCTGGGCATGGTGGAGTCCGGCCAGTGGCGACGCCGCTTCGACGAGCGCAGCGATAAGGATCAAAGCTGGGAGCAGTGGA
CGGCGGTGATTGCCGAACGTCGCGGCATCCCGATGAAACGCCTCGGTAAGCCACAGGAGCCGGCGCAAGCGCTGTTGTTC
CTGGCTTCGCCTTTGGCGTCTTTCACTACCGGTGCGGCGCTTGACGTCTCCGGTGGCTTTAACCGCCATCTTTAA

Upstream 100 bases:

>100_bases
CGCCTCTGAGGTTGATTGAGGGCGCAGGGCACGCCAGCTATCTCGATGCGCCGGAGCAATTTAACCGTTATCTGCAGGAC
TTTACGGGAGCAATCCAACC

Downstream 100 bases:

>100_bases
CCGCAGGGGCTGAAAAGATGAAGAAGATCATGATGATTGGCTACGGCGCAATGGCCAGAGAAGTGCTTTCCCGCTTGCCG
GACGGGGTGAGTGTGGGCTG

Product: short chain dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MNFQLEQRVAVVTGGSSGIGFETLRLLLAEGAKVAFCGRNPDKLAGAEASLRNEFPQAEILALCCDVLDEAQVTQFAAQV
TAHFGGVDLLINNAGQGFVAHFDQTPRSAWLHEAELKLFGVINPVQAFLPALERSDIASITCVNSLLALQPEEHMIATSA
ARAALLNMTLTLSKELVDKGIRVNSILLGMVESGQWRRRFDERSDKDQSWEQWTAVIAERRGIPMKRLGKPQEPAQALLF
LASPLASFTTGAALDVSGGFNRHL

Sequences:

>Translated_264_residues
MNFQLEQRVAVVTGGSSGIGFETLRLLLAEGAKVAFCGRNPDKLAGAEASLRNEFPQAEILALCCDVLDEAQVTQFAAQV
TAHFGGVDLLINNAGQGFVAHFDQTPRSAWLHEAELKLFGVINPVQAFLPALERSDIASITCVNSLLALQPEEHMIATSA
ARAALLNMTLTLSKELVDKGIRVNSILLGMVESGQWRRRFDERSDKDQSWEQWTAVIAERRGIPMKRLGKPQEPAQALLF
LASPLASFTTGAALDVSGGFNRHL
>Mature_264_residues
MNFQLEQRVAVVTGGSSGIGFETLRLLLAEGAKVAFCGRNPDKLAGAEASLRNEFPQAEILALCCDVLDEAQVTQFAAQV
TAHFGGVDLLINNAGQGFVAHFDQTPRSAWLHEAELKLFGVINPVQAFLPALERSDIASITCVNSLLALQPEEHMIATSA
ARAALLNMTLTLSKELVDKGIRVNSILLGMVESGQWRRRFDERSDKDQSWEQWTAVIAERRGIPMKRLGKPQEPAQALLF
LASPLASFTTGAALDVSGGFNRHL

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI19923817, Length=266, Percent_Identity=29.6992481203008, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI32483357, Length=261, Percent_Identity=26.8199233716475, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI5031737, Length=260, Percent_Identity=28.8461538461538, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI10190704, Length=255, Percent_Identity=28.6274509803922, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI40254992, Length=254, Percent_Identity=27.9527559055118, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI33667109, Length=190, Percent_Identity=32.1052631578947, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI59889578, Length=245, Percent_Identity=27.7551020408163, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI126723750, Length=259, Percent_Identity=25.8687258687259, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI31542939, Length=191, Percent_Identity=28.7958115183246, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI15277342, Length=275, Percent_Identity=28, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI7705925, Length=263, Percent_Identity=28.5171102661597, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI126723191, Length=187, Percent_Identity=26.2032085561497, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787905, Length=260, Percent_Identity=30, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1787335, Length=260, Percent_Identity=30.3846153846154, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786812, Length=251, Percent_Identity=31.0756972111554, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1789057, Length=268, Percent_Identity=28.3582089552239, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789378, Length=257, Percent_Identity=28.4046692607004, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082100, Length=263, Percent_Identity=30.0380228136882, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789208, Length=262, Percent_Identity=26.3358778625954, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790717, Length=258, Percent_Identity=23.2558139534884, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1788459, Length=264, Percent_Identity=26.8939393939394, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17560676, Length=260, Percent_Identity=28.4615384615385, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17560332, Length=265, Percent_Identity=30.188679245283, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25147288, Length=264, Percent_Identity=29.5454545454545, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17562906, Length=262, Percent_Identity=28.6259541984733, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17560150, Length=260, Percent_Identity=29.6153846153846, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17562904, Length=263, Percent_Identity=28.8973384030418, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17551412, Length=266, Percent_Identity=28.9473684210526, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17531453, Length=269, Percent_Identity=27.5092936802974, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17562910, Length=263, Percent_Identity=28.8973384030418, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17565030, Length=271, Percent_Identity=30.9963099630996, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17559104, Length=261, Percent_Identity=27.9693486590038, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17562990, Length=256, Percent_Identity=28.515625, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17538480, Length=272, Percent_Identity=29.0441176470588, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI72000259, Length=259, Percent_Identity=27.7992277992278, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17562908, Length=264, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI193204405, Length=270, Percent_Identity=27.4074074074074, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17544670, Length=263, Percent_Identity=25.8555133079848, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17538486, Length=268, Percent_Identity=27.2388059701493, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17563726, Length=265, Percent_Identity=27.1698113207547, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17561402, Length=264, Percent_Identity=24.6212121212121, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560220, Length=270, Percent_Identity=29.2592592592593, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17555706, Length=261, Percent_Identity=27.2030651340996, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17536025, Length=260, Percent_Identity=26.1538461538462, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI115534694, Length=266, Percent_Identity=29.6992481203008, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71994604, Length=254, Percent_Identity=26.3779527559055, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71994600, Length=253, Percent_Identity=25.6916996047431, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17558006, Length=204, Percent_Identity=32.3529411764706, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17536651, Length=266, Percent_Identity=25.5639097744361, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17561272, Length=237, Percent_Identity=26.1603375527426, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=257, Percent_Identity=26.8482490272374, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323882, Length=222, Percent_Identity=25.6756756756757, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI23397609, Length=263, Percent_Identity=33.8403041825095, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24639444, Length=261, Percent_Identity=31.8007662835249, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355319, Length=265, Percent_Identity=28.3018867924528, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21357041, Length=266, Percent_Identity=28.9473684210526, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28571526, Length=259, Percent_Identity=28.957528957529, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24644339, Length=263, Percent_Identity=26.9961977186312, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24643142, Length=257, Percent_Identity=29.5719844357977, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24644337, Length=196, Percent_Identity=29.0816326530612, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21357673, Length=222, Percent_Identity=26.5765765765766, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28571387, Length=201, Percent_Identity=30.3482587064677, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 28668; Mature: 28668

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFQLEQRVAVVTGGSSGIGFETLRLLLAEGAKVAFCGRNPDKLAGAEASLRNEFPQAEI
CCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCHHHHCCCHHHHHHCCCHHHH
LALCCDVLDEAQVTQFAAQVTAHFGGVDLLINNAGQGFVAHFDQTPRSAWLHEAELKLFG
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHEEE
VINPVQAFLPALERSDIASITCVNSLLALQPEEHMIATSAARAALLNMTLTLSKELVDKG
CCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IRVNSILLGMVESGQWRRRFDERSDKDQSWEQWTAVIAERRGIPMKRLGKPQEPAQALLF
CCHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHH
LASPLASFTTGAALDVSGGFNRHL
HHHHHHHHHCCCEEECCCCCCCCC
>Mature Secondary Structure
MNFQLEQRVAVVTGGSSGIGFETLRLLLAEGAKVAFCGRNPDKLAGAEASLRNEFPQAEI
CCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCHHHHCCCHHHHHHCCCHHHH
LALCCDVLDEAQVTQFAAQVTAHFGGVDLLINNAGQGFVAHFDQTPRSAWLHEAELKLFG
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHEEE
VINPVQAFLPALERSDIASITCVNSLLALQPEEHMIATSAARAALLNMTLTLSKELVDKG
CCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IRVNSILLGMVESGQWRRRFDERSDKDQSWEQWTAVIAERRGIPMKRLGKPQEPAQALLF
CCHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHH
LASPLASFTTGAALDVSGGFNRHL
HHHHHHHHHCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]