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The map label for this gene is nadE [H]

Identifier: 157371885

GI number: 157371885

Start: 4037435

End: 4039057

Strand: Reverse

Name: nadE [H]

Synonym: Spro_3650

Alternate gene names: 157371885

Gene position: 4039057-4037435 (Counterclockwise)

Preceding gene: 157371886

Following gene: 157371884

Centisome position: 74.13

GC content: 57.67

Gene sequence:

>1623_bases
ATGAGCAGATCACTTTCCATCGCACTGGCCCAGCTGAATCTGCTGGTCGGTGACATTGAAGGCAACACCGAACGCATGTT
GCAAACCGTGCAAGAGCAGCAGAAGGCGGGAGCCGATCTGGTCATGTTCACCGAGTTGGCGCTCTCCGGCTATCCGCCGG
AAGACCTGCTGTACCGCAACGATTTTTACCAGCGCTGTGACGCGCAGCTGCAGCGTCTGCAGCAGGCATCTGCCGAGGTC
GCCATTCTGGTTGGCCATCCGTGGCGTGAAGGCGACAAGCTGTATAATGCGCTGTCGCTGTTTTCTGAAGGGCAGTTGCT
GGCGCGTTACTTCAAGCAGCAGTTGCCGAACTACGGCGTTTTCGATGAGAAACGCTACTTCCAGGCCGGTAGTGAAACCT
GCGTGGTTGAGCTGAAAGGCTATCAATTGGGCCTGCTGATTTGCGAAGATCTGTGGTTCCCAGGGCCGGTCGATGCCGCC
AAGGCGGCCGGTGCCGAAATGATTTTGTCGATCAACGCCTCGCCGTACAACCGCGAAAAACCGTATATCCGCAAAACGTT
AATGGCCGGCCATTGCCAACGTACCCATCTGCCGCTGGTGTATCTGAACCAGATTGGCGGGCAGGATGAGCTGATTTTCG
ACGGCTGTTCGAAGGTGTTCGATGCGGCCGGTAACATGACCCATCGCCTGACGGCGTTTGCCGAGCAGGTGACCCTGCTT
GAGCTCAATGAGCTGGAGGTGGTGCCAATGACCGCTCCGGCGGCCGAGCTGCCACAGTTGGCGCAGGTGTACGAAGCCCT
GGTGTTGGCGGTGCGCGATTACGTCACTAAAAACGGCTTTAAGGGCGCGGTACTGGGGTTGTCTGGCGGTATCGATTCGG
CGCTGACGCTGGCGATTGCCGTCGATGCCTTGGGCAAAGATAAAGTGCAGGCGCTGATGATGCCGTTCCGCTACACCGCG
GATATCAGTATCGCTGACGCCAAAGAAGAAGCTGAAATCCTCGGCATTGAATTCGACATCGTCTCTATTGAACCTATGTT
CGACGCCTTTATGGGCCAGCTGACGCCAATGTTTGCCGGTACCGAACGCGACACCACCGAAGAGAACCTGCAGGCACGCT
GCCGCGGCGTGGTGTTGATGGCGCTGTCCAACAAGCGCCGCAGCATCGTGTTGACCACCGGCAACAAAAGCGAGATGGCC
GTAGGTTACGCCACGCTGTATGGTGATATGGCAGGTGGCTTCGACGTGCTGAAAGACGTACCGAAAACGCTGGTGTTCAA
GCTTTCCGAATACCGTAACACCGTCTCATACGTGATCCCGCAGCGGGTTATCGATCGTCCGCCGTCCGCAGAGCTGGCGC
CGGATCAGCTCGATCAGGACAGCCTGCCGCCGTACGACATTCTGGATGCAATTCTGGAAGGTTACGTCGAGCGCGATAAG
TCGGTTGCCGATCTGGTGGCCGAAGGCTTCGACGAGGCGATCGTGCGCAAGGTTATCCGTCTGGTGGATATCAACGAATA
CAAACGGCGTCAGGCCGCCGTCGGGCCACGCATCACCGCCCGCAATTTCGGCAAAGACCGCCGCTACCCAATCACATCCG
GCTTTGGCCGCAAAAATTGGTAA

Upstream 100 bases:

>100_bases
TTCTCAACAAATGCGGCGGAATGATTTACACTCCGTTCTCTTGTTTGTGGCGTGGCCCAACACAGAGCACCATGGAACGT
ATTAAGCGGGATCCTAAACA

Downstream 100 bases:

>100_bases
AGGAAACAAGATGAAAAAGATCGACGCTATTATCAAGCCGTTCAAACTGGATGATGTCCGTGAAGCGCTGGCTGAGGTTG
GCATTACCGGGATGACCGTC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSRSLSIALAQLNLLVGDIEGNTERMLQTVQEQQKAGADLVMFTELALSGYPPEDLLYRNDFYQRCDAQLQRLQQASAEV
AILVGHPWREGDKLYNALSLFSEGQLLARYFKQQLPNYGVFDEKRYFQAGSETCVVELKGYQLGLLICEDLWFPGPVDAA
KAAGAEMILSINASPYNREKPYIRKTLMAGHCQRTHLPLVYLNQIGGQDELIFDGCSKVFDAAGNMTHRLTAFAEQVTLL
ELNELEVVPMTAPAAELPQLAQVYEALVLAVRDYVTKNGFKGAVLGLSGGIDSALTLAIAVDALGKDKVQALMMPFRYTA
DISIADAKEEAEILGIEFDIVSIEPMFDAFMGQLTPMFAGTERDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMA
VGYATLYGDMAGGFDVLKDVPKTLVFKLSEYRNTVSYVIPQRVIDRPPSAELAPDQLDQDSLPPYDILDAILEGYVERDK
SVADLVAEGFDEAIVRKVIRLVDINEYKRRQAAVGPRITARNFGKDRRYPITSGFGRKNW

Sequences:

>Translated_540_residues
MSRSLSIALAQLNLLVGDIEGNTERMLQTVQEQQKAGADLVMFTELALSGYPPEDLLYRNDFYQRCDAQLQRLQQASAEV
AILVGHPWREGDKLYNALSLFSEGQLLARYFKQQLPNYGVFDEKRYFQAGSETCVVELKGYQLGLLICEDLWFPGPVDAA
KAAGAEMILSINASPYNREKPYIRKTLMAGHCQRTHLPLVYLNQIGGQDELIFDGCSKVFDAAGNMTHRLTAFAEQVTLL
ELNELEVVPMTAPAAELPQLAQVYEALVLAVRDYVTKNGFKGAVLGLSGGIDSALTLAIAVDALGKDKVQALMMPFRYTA
DISIADAKEEAEILGIEFDIVSIEPMFDAFMGQLTPMFAGTERDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMA
VGYATLYGDMAGGFDVLKDVPKTLVFKLSEYRNTVSYVIPQRVIDRPPSAELAPDQLDQDSLPPYDILDAILEGYVERDK
SVADLVAEGFDEAIVRKVIRLVDINEYKRRQAAVGPRITARNFGKDRRYPITSGFGRKNW
>Mature_539_residues
SRSLSIALAQLNLLVGDIEGNTERMLQTVQEQQKAGADLVMFTELALSGYPPEDLLYRNDFYQRCDAQLQRLQQASAEVA
ILVGHPWREGDKLYNALSLFSEGQLLARYFKQQLPNYGVFDEKRYFQAGSETCVVELKGYQLGLLICEDLWFPGPVDAAK
AAGAEMILSINASPYNREKPYIRKTLMAGHCQRTHLPLVYLNQIGGQDELIFDGCSKVFDAAGNMTHRLTAFAEQVTLLE
LNELEVVPMTAPAAELPQLAQVYEALVLAVRDYVTKNGFKGAVLGLSGGIDSALTLAIAVDALGKDKVQALMMPFRYTAD
ISIADAKEEAEILGIEFDIVSIEPMFDAFMGQLTPMFAGTERDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMAV
GYATLYGDMAGGFDVLKDVPKTLVFKLSEYRNTVSYVIPQRVIDRPPSAELAPDQLDQDSLPPYDILDAILEGYVERDKS
VADLVAEGFDEAIVRKVIRLVDINEYKRRQAAVGPRITARNFGKDRRYPITSGFGRKNW

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6321865, Length=299, Percent_Identity=26.7558528428094, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 59946; Mature: 59815

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSLSIALAQLNLLVGDIEGNTERMLQTVQEQQKAGADLVMFTELALSGYPPEDLLYRN
CCCCHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCHHHHHHH
DFYQRCDAQLQRLQQASAEVAILVGHPWREGDKLYNALSLFSEGQLLARYFKQQLPNYGV
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
FDEKRYFQAGSETCVVELKGYQLGLLICEDLWFPGPVDAAKAAGAEMILSINASPYNREK
CCCHHHHHCCCCEEEEEECCCEEEEEEEHHCCCCCCCCHHHHCCCEEEEEECCCCCCCCC
PYIRKTLMAGHCQRTHLPLVYLNQIGGQDELIFDGCSKVFDAAGNMTHRLTAFAEQVTLL
CHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHEEE
ELNELEVVPMTAPAAELPQLAQVYEALVLAVRDYVTKNGFKGAVLGLSGGIDSALTLAIA
EECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHH
VDALGKDKVQALMMPFRYTADISIADAKEEAEILGIEFDIVSIEPMFDAFMGQLTPMFAG
HHHCCHHHHHHHHHCCEEECCEEEECCCCCCEEEEEEEEEEEECHHHHHHHHHHHHHHCC
TERDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMAVGYATLYGDMAGGFDVLKDV
CCCCCCHHHHHHHHCEEEEEEECCCCCEEEEEECCCCCCEEEHHHHHHCCCCCHHHHHHH
PKTLVFKLSEYRNTVSYVIPQRVIDRPPSAELAPDQLDQDSLPPYDILDAILEGYVERDK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCH
SVADLVAEGFDEAIVRKVIRLVDINEYKRRQAAVGPRITARNFGKDRRYPITSGFGRKNW
HHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRSLSIALAQLNLLVGDIEGNTERMLQTVQEQQKAGADLVMFTELALSGYPPEDLLYRN
CCCHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCHHHHHHH
DFYQRCDAQLQRLQQASAEVAILVGHPWREGDKLYNALSLFSEGQLLARYFKQQLPNYGV
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
FDEKRYFQAGSETCVVELKGYQLGLLICEDLWFPGPVDAAKAAGAEMILSINASPYNREK
CCCHHHHHCCCCEEEEEECCCEEEEEEEHHCCCCCCCCHHHHCCCEEEEEECCCCCCCCC
PYIRKTLMAGHCQRTHLPLVYLNQIGGQDELIFDGCSKVFDAAGNMTHRLTAFAEQVTLL
CHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHEEE
ELNELEVVPMTAPAAELPQLAQVYEALVLAVRDYVTKNGFKGAVLGLSGGIDSALTLAIA
EECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHH
VDALGKDKVQALMMPFRYTADISIADAKEEAEILGIEFDIVSIEPMFDAFMGQLTPMFAG
HHHCCHHHHHHHHHCCEEECCEEEECCCCCCEEEEEEEEEEEECHHHHHHHHHHHHHHCC
TERDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMAVGYATLYGDMAGGFDVLKDV
CCCCCCHHHHHHHHCEEEEEEECCCCCEEEEEECCCCCCEEEHHHHHHCCCCCHHHHHHH
PKTLVFKLSEYRNTVSYVIPQRVIDRPPSAELAPDQLDQDSLPPYDILDAILEGYVERDK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCH
SVADLVAEGFDEAIVRKVIRLVDINEYKRRQAAVGPRITARNFGKDRRYPITSGFGRKNW
HHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]