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The map label for this gene is ppx [H]

Identifier: 157371777

GI number: 157371777

Start: 3912317

End: 3913867

Strand: Direct

Name: ppx [H]

Synonym: Spro_3542

Alternate gene names: 157371777

Gene position: 3912317-3913867 (Clockwise)

Preceding gene: 157371776

Following gene: 157371779

Centisome position: 71.8

GC content: 57.25

Gene sequence:

>1551_bases
ATGCCGCTAAAAAGCACTGCAACAAATAAACCGCAGGAAATTGCTGCCATCGACCTCGGGTCGAACAGCTTCCATATGGT
GATTGCCCGCGTCGTCAACGGCGCTTTACAGGTATTGGGCCGTTTAAAACAACGGGTCCACCTCGCCGATGGATTGGACA
GCAACAATGTGCTCAGTGAAGAGGCAATAGAACGTGGACTGGCCTGCCTGGCGCTGTTTGCCGAACGGCTGCAGGGCTTC
CCGGCGGATAACGTCACCATCGTCGGTACTCACACCCTGCGTCAGGCCGTTAACGCGGAAGTGTTTCTCAAACGTGCAGC
TAAAGTGATCCCCTACCCGATTGAAATCATCGCCGGACAGGAAGAAGCGCGTCTGATCTTTATGGGCGTGGAGCACACCC
AGCCGGAAAAAGGCCGCAAGCTGGTGATCGATATCGGCGGTGGTTCTACTGAGCTGGTGATCGGTGAAGACTTTGAGCCG
CTGCTGGCAGAAAGCCGCCGTATGGGCTGCGTCAGTTTCGCCCAGCAGTTTTTCCCAGGCGGTGAAATCAGTAAAAACAA
TTTCAAGCGTGCGCGCCTGGCAGCGGCACAGAAGCTGGAAACCCTGGCCTGGCAGTACCGTATTCAGGGTTGGCAGTATG
CTCTGGGCGCGTCCGGTACCATCAAGGCCGCACACGAAGTGCTGGTGGCGATGGGCGAAAAAGACGGCCTGATCACCCTG
GAACGGCTGGAGATGCTGACGGAGCAGGTGTTGCAGTTTAAAAGCTTCAGCTCCCTGAGCCTGCCGGGGCTGTCGGAAGA
CCGTCAGTCGGTGTTTGTACCGGGTCTGGCTATCCTGTGCGGCGTGTTCGACGCGTTGGCAATCCGCGACCTGCGTCTTT
CTGACGGCGCGCTGCGCGAAGGCGTGCTGTACGAAATGGAGGGCCGTTTCCGCCATCAGGATATCCGCAGCCGTACCGCC
AAAAGCCTGGCCGATCACTACAATATCGACCGTGAGCAGGCCAAGCGGGTGCTGGACACCACCGAACTGCTGTACGCCCA
ATGGATGGCGCAAAACACCAAGCTGGCAAATCCGCAGTTGGAAGCGCTGTTGAAATGGGCCGCCATGTTGCATGAGGTGG
GGCTGAGCATCAACCACAGCGGCATGCATCGCCACTCGGCCTATATTCTGCAAAACACCAACCTGCCCGGTTTTAATCAG
GAGCAGCAGTTGCTGCTGGCGGCGCTGGTGCGTTTCCACCGCAAGGGGATCAAGCTGGAAGAACTGCCGCGGCTGAACCT
GTTCAAGAAAAAGCATTACCTGCCGCTGATCCAACTGCTGCGTTTGAGCACCCTGCTCAACAACCAGCGTCAGTCGACCA
CCACACCCGAGACGCTGCGCCTGAGTACTGACGATAATCACTGGACGCTGCGTTTCCCGGCCGGTTATCTGGCGTTAAAC
AACCTGGTACAACTGGATCTTGAACGTGAGCAGGCCTACTGGAATGACGTCGTCGGCTGGAAGCTGATTTGCGAAGAAGA
AGAGACCCAAAACGAGCAACGTACTGCCTGA

Upstream 100 bases:

>100_bases
CAGTACGTACCGCGCGGCAACCGCCGTAAAGTCCGTTCTCAGGTTGCCATTTACGAGTATTTAAAAGCTCTGGAACAACC
AGGACAGTAGGCCAGCAACT

Downstream 100 bases:

>100_bases
GTCTTTCGCCCCCTGCCTGCCGGGGGGCAACACTCAACGCGGTGTCGGTAACAGCGCCAGCAAGGGTTGTGGCTCGCCAA
TGAAGTAGCCCTGCATATAG

Product: exopolyphosphatase

Products: NA

Alternate protein names: ExopolyPase; Metaphosphatase [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MPLKSTATNKPQEIAAIDLGSNSFHMVIARVVNGALQVLGRLKQRVHLADGLDSNNVLSEEAIERGLACLALFAERLQGF
PADNVTIVGTHTLRQAVNAEVFLKRAAKVIPYPIEIIAGQEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGEDFEP
LLAESRRMGCVSFAQQFFPGGEISKNNFKRARLAAAQKLETLAWQYRIQGWQYALGASGTIKAAHEVLVAMGEKDGLITL
ERLEMLTEQVLQFKSFSSLSLPGLSEDRQSVFVPGLAILCGVFDALAIRDLRLSDGALREGVLYEMEGRFRHQDIRSRTA
KSLADHYNIDREQAKRVLDTTELLYAQWMAQNTKLANPQLEALLKWAAMLHEVGLSINHSGMHRHSAYILQNTNLPGFNQ
EQQLLLAALVRFHRKGIKLEELPRLNLFKKKHYLPLIQLLRLSTLLNNQRQSTTTPETLRLSTDDNHWTLRFPAGYLALN
NLVQLDLEREQAYWNDVVGWKLICEEEETQNEQRTA

Sequences:

>Translated_516_residues
MPLKSTATNKPQEIAAIDLGSNSFHMVIARVVNGALQVLGRLKQRVHLADGLDSNNVLSEEAIERGLACLALFAERLQGF
PADNVTIVGTHTLRQAVNAEVFLKRAAKVIPYPIEIIAGQEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGEDFEP
LLAESRRMGCVSFAQQFFPGGEISKNNFKRARLAAAQKLETLAWQYRIQGWQYALGASGTIKAAHEVLVAMGEKDGLITL
ERLEMLTEQVLQFKSFSSLSLPGLSEDRQSVFVPGLAILCGVFDALAIRDLRLSDGALREGVLYEMEGRFRHQDIRSRTA
KSLADHYNIDREQAKRVLDTTELLYAQWMAQNTKLANPQLEALLKWAAMLHEVGLSINHSGMHRHSAYILQNTNLPGFNQ
EQQLLLAALVRFHRKGIKLEELPRLNLFKKKHYLPLIQLLRLSTLLNNQRQSTTTPETLRLSTDDNHWTLRFPAGYLALN
NLVQLDLEREQAYWNDVVGWKLICEEEETQNEQRTA
>Mature_515_residues
PLKSTATNKPQEIAAIDLGSNSFHMVIARVVNGALQVLGRLKQRVHLADGLDSNNVLSEEAIERGLACLALFAERLQGFP
ADNVTIVGTHTLRQAVNAEVFLKRAAKVIPYPIEIIAGQEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGEDFEPL
LAESRRMGCVSFAQQFFPGGEISKNNFKRARLAAAQKLETLAWQYRIQGWQYALGASGTIKAAHEVLVAMGEKDGLITLE
RLEMLTEQVLQFKSFSSLSLPGLSEDRQSVFVPGLAILCGVFDALAIRDLRLSDGALREGVLYEMEGRFRHQDIRSRTAK
SLADHYNIDREQAKRVLDTTELLYAQWMAQNTKLANPQLEALLKWAAMLHEVGLSINHSGMHRHSAYILQNTNLPGFNQE
QQLLLAALVRFHRKGIKLEELPRLNLFKKKHYLPLIQLLRLSTLLNNQRQSTTTPETLRLSTDDNHWTLRFPAGYLALNN
LVQLDLEREQAYWNDVVGWKLICEEEETQNEQRTA

Specific function: Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate [H]

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family [H]

Homologues:

Organism=Escherichia coli, GI1788848, Length=509, Percent_Identity=76.2278978388998, Blast_Score=795, Evalue=0.0,
Organism=Escherichia coli, GI48994966, Length=488, Percent_Identity=39.5491803278689, Blast_Score=333, Evalue=2e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022371
- InterPro:   IPR003695 [H]

Pfam domain/function: PF02541 Ppx-GppA [H]

EC number: =3.6.1.11 [H]

Molecular weight: Translated: 58014; Mature: 57883

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLKSTATNKPQEIAAIDLGSNSFHMVIARVVNGALQVLGRLKQRVHLADGLDSNNVLSE
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
EAIERGLACLALFAERLQGFPADNVTIVGTHTLRQAVNAEVFLKRAAKVIPYPIEIIAGQ
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
EEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGEDFEPLLAESRRMGCVSFAQQFFPG
CCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHCCHHHHHHHHHCCC
GEISKNNFKRARLAAAQKLETLAWQYRIQGWQYALGASGTIKAAHEVLVAMGEKDGLITL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEH
ERLEMLTEQVLQFKSFSSLSLPGLSEDRQSVFVPGLAILCGVFDALAIRDLRLSDGALRE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHCCCCCCCHHC
GVLYEMEGRFRHQDIRSRTAKSLADHYNIDREQAKRVLDTTELLYAQWMAQNTKLANPQL
CCEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
EALLKWAAMLHEVGLSINHSGMHRHSAYILQNTNLPGFNQEQQLLLAALVRFHRKGIKLE
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHH
ELPRLNLFKKKHYLPLIQLLRLSTLLNNQRQSTTTPETLRLSTDDNHWTLRFPAGYLALN
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEECCCHHHHHH
NLVQLDLEREQAYWNDVVGWKLICEEEETQNEQRTA
HHHEEECHHHHHHHHHCCCEEEEECCHHCCHHHCCC
>Mature Secondary Structure 
PLKSTATNKPQEIAAIDLGSNSFHMVIARVVNGALQVLGRLKQRVHLADGLDSNNVLSE
CCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
EAIERGLACLALFAERLQGFPADNVTIVGTHTLRQAVNAEVFLKRAAKVIPYPIEIIAGQ
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
EEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGEDFEPLLAESRRMGCVSFAQQFFPG
CCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHCCHHHHHHHHHCCC
GEISKNNFKRARLAAAQKLETLAWQYRIQGWQYALGASGTIKAAHEVLVAMGEKDGLITL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEH
ERLEMLTEQVLQFKSFSSLSLPGLSEDRQSVFVPGLAILCGVFDALAIRDLRLSDGALRE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHCCCCCCCHHC
GVLYEMEGRFRHQDIRSRTAKSLADHYNIDREQAKRVLDTTELLYAQWMAQNTKLANPQL
CCEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
EALLKWAAMLHEVGLSINHSGMHRHSAYILQNTNLPGFNQEQQLLLAALVRFHRKGIKLE
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHH
ELPRLNLFKKKHYLPLIQLLRLSTLLNNQRQSTTTPETLRLSTDDNHWTLRFPAGYLALN
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEECCCHHHHHH
NLVQLDLEREQAYWNDVVGWKLICEEEETQNEQRTA
HHHEEECHHHHHHHHHCCCEEEEECCHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]