Click here to switch to the map view.

The map label for this gene is acrD [H]

Identifier: 157371730

GI number: 157371730

Start: 3867599

End: 3868696

Strand: Direct

Name: acrD [H]

Synonym: Spro_3493

Alternate gene names: 157371730

Gene position: 3867599-3868696 (Clockwise)

Preceding gene: 157371729

Following gene: 157371732

Centisome position: 70.98

GC content: 59.65

Gene sequence:

>1098_bases
ATGGAACTCGAAGACCACGCCGGCCTGGGCCACGACAAACTGATGGCGGCACGCAATCAACTGCTGCAACTGGCCGCCGC
TAACCCGCTGCTGTCCCGCGTGCGCCACAATGGCCTGGATGACAGCCCGCAGCTGCAAATTGATATTGACCAACGCAAAG
CCCAGGCGCTGGGTGTTTCAATTGATGACATTAACAACACGCTGTCGACCGCCTGGGGCTCCACCTACGTTAACGACTTC
GTCGACCGCGGTCGGGTGAAAAAAGTCTATGTGCAGGCCGCCGCACCGTTCCGCATGCTGCCTGGCGACATCGATAAATG
GTTCGTGCGCAACAGCGCGGGTGGCATGGTGCCGTTCAGCGCCTTTGCCACCTCCCACTGGGAATATGGCTCCCCACGTC
TGGAACGCTATAACGGCAGCTCAGCGCTGGAAATTGTGGGCGAAGCGGCAGAGGGTGTCAGCAGTGGTACCGCAATGGCC
GAAATGGAAAAGATTGTCAGCCAGTTACCGACCGGCTTCGGTCTGGAATGGACCGCCATGTCCTACCAGGAACGCCTGTC
CGGCTCGCAGGCACCGGCGCTGTATGCCATTTCGCTGTTGGTGGTGTTCCTGTGTCTGGCGGCGCTGTATGAGAGCTGGT
CGATCCCGTTCTCGGTGATGCTGGTGGTGCCACTGGGCCTGATTGGTGCCGTTGCCGCTACCTGGCTGCGCGGACTGGAA
AATGACGTTTACTTCCAGGTCGGGATGCTGACCATCATCGGGCTGTCGGCGAAAAACGCCATTTTGATCGTCGAATTCGC
CAACGAGCTAAACCAGAAAGGTCAGGATTTGATGTCAGCCACATTGGAAGCCTCACGCATGCGCCTGCGGCCGATCCTGA
TGACCTCGCTGGCCTTTATCTTCGGCGTACTGCCGATGACCATCAGCAGTGGGGCCGGCTCAGGCAGCCAGCATGCGGTG
GGTACCGGGGTAATGGGCGGTATGATTTCCGCCACCCTGCTGGCAATTTACTTCGTACCGCTGTTCTTTGTGCTGGTACG
CCGCCGCTTCCCAGGCAAGCCGGCAGCCACCGCCAAAGCGCAAGACGTCGCGCAATAG

Upstream 100 bases:

>100_bases
TCGATCGCGCCACCAAGGAATTTAACCAAATTATCGAGGCGCGGGTGATTGCCAGCAGTCCACCGGCGATTACCGGTCTG
GCAATTCTTCCGGCTTCGAT

Downstream 100 bases:

>100_bases
CAGGCAAAAAAAAGCAGCGTCGGGAAACCGATGCTGCTTTATCTCGAACGAGTGTCTCGTTCCCAGGGTGCTTTAATCAC
GAACAAATAAACCAACCTTG

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Acriflavine resistance protein D [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MELEDHAGLGHDKLMAARNQLLQLAAANPLLSRVRHNGLDDSPQLQIDIDQRKAQALGVSIDDINNTLSTAWGSTYVNDF
VDRGRVKKVYVQAAAPFRMLPGDIDKWFVRNSAGGMVPFSAFATSHWEYGSPRLERYNGSSALEIVGEAAEGVSSGTAMA
EMEKIVSQLPTGFGLEWTAMSYQERLSGSQAPALYAISLLVVFLCLAALYESWSIPFSVMLVVPLGLIGAVAATWLRGLE
NDVYFQVGMLTIIGLSAKNAILIVEFANELNQKGQDLMSATLEASRMRLRPILMTSLAFIFGVLPMTISSGAGSGSQHAV
GTGVMGGMISATLLAIYFVPLFFVLVRRRFPGKPAATAKAQDVAQ

Sequences:

>Translated_365_residues
MELEDHAGLGHDKLMAARNQLLQLAAANPLLSRVRHNGLDDSPQLQIDIDQRKAQALGVSIDDINNTLSTAWGSTYVNDF
VDRGRVKKVYVQAAAPFRMLPGDIDKWFVRNSAGGMVPFSAFATSHWEYGSPRLERYNGSSALEIVGEAAEGVSSGTAMA
EMEKIVSQLPTGFGLEWTAMSYQERLSGSQAPALYAISLLVVFLCLAALYESWSIPFSVMLVVPLGLIGAVAATWLRGLE
NDVYFQVGMLTIIGLSAKNAILIVEFANELNQKGQDLMSATLEASRMRLRPILMTSLAFIFGVLPMTISSGAGSGSQHAV
GTGVMGGMISATLLAIYFVPLFFVLVRRRFPGKPAATAKAQDVAQ
>Mature_365_residues
MELEDHAGLGHDKLMAARNQLLQLAAANPLLSRVRHNGLDDSPQLQIDIDQRKAQALGVSIDDINNTLSTAWGSTYVNDF
VDRGRVKKVYVQAAAPFRMLPGDIDKWFVRNSAGGMVPFSAFATSHWEYGSPRLERYNGSSALEIVGEAAEGVSSGTAMA
EMEKIVSQLPTGFGLEWTAMSYQERLSGSQAPALYAISLLVVFLCLAALYESWSIPFSVMLVVPLGLIGAVAATWLRGLE
NDVYFQVGMLTIIGLSAKNAILIVEFANELNQKGQDLMSATLEASRMRLRPILMTSLAFIFGVLPMTISSGAGSGSQHAV
GTGVMGGMISATLLAIYFVPLFFVLVRRRFPGKPAATAKAQDVAQ

Specific function: Participates in the efflux of aminoglycosides. Confers resistance to a variety of these substances [H]

COG id: COG0841

COG function: function code V; Cation/multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AcrB/AcrD/AcrF (TC 2.A.6) family [H]

Homologues:

Organism=Escherichia coli, GI1788814, Length=354, Percent_Identity=85.3107344632768, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI1786667, Length=337, Percent_Identity=71.513353115727, Blast_Score=497, Evalue=1e-142,
Organism=Escherichia coli, GI1789666, Length=353, Percent_Identity=69.971671388102, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1789930, Length=339, Percent_Identity=66.3716814159292, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI1788391, Length=359, Percent_Identity=28.6908077994429, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788390, Length=337, Percent_Identity=27.0029673590504, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1786788, Length=346, Percent_Identity=20.2312138728324, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001036
- InterPro:   IPR004764 [H]

Pfam domain/function: PF00873 ACR_tran [H]

EC number: NA

Molecular weight: Translated: 39351; Mature: 39351

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELEDHAGLGHDKLMAARNQLLQLAAANPLLSRVRHNGLDDSPQLQIDIDQRKAQALGVS
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECHHHHHHHCCC
IDDINNTLSTAWGSTYVNDFVDRGRVKKVYVQAAAPFRMLPGDIDKWFVRNSAGGMVPFS
HHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCCCCCCHH
AFATSHWEYGSPRLERYNGSSALEIVGEAAEGVSSGTAMAEMEKIVSQLPTGFGLEWTAM
HHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEHHH
SYQERLSGSQAPALYAISLLVVFLCLAALYESWSIPFSVMLVVPLGLIGAVAATWLRGLE
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
NDVYFQVGMLTIIGLSAKNAILIVEFANELNQKGQDLMSATLEASRMRLRPILMTSLAFI
CCHHEEEHHHHHEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGVLPMTISSGAGSGSQHAVGTGVMGGMISATLLAIYFVPLFFVLVRRRFPGKPAATAKA
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
QDVAQ
HHCCC
>Mature Secondary Structure
MELEDHAGLGHDKLMAARNQLLQLAAANPLLSRVRHNGLDDSPQLQIDIDQRKAQALGVS
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECHHHHHHHCCC
IDDINNTLSTAWGSTYVNDFVDRGRVKKVYVQAAAPFRMLPGDIDKWFVRNSAGGMVPFS
HHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCCCCCCHH
AFATSHWEYGSPRLERYNGSSALEIVGEAAEGVSSGTAMAEMEKIVSQLPTGFGLEWTAM
HHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEHHH
SYQERLSGSQAPALYAISLLVVFLCLAALYESWSIPFSVMLVVPLGLIGAVAATWLRGLE
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
NDVYFQVGMLTIIGLSAKNAILIVEFANELNQKGQDLMSATLEASRMRLRPILMTSLAFI
CCHHEEEHHHHHEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGVLPMTISSGAGSGSQHAVGTGVMGGMISATLLAIYFVPLFFVLVRRRFPGKPAATAKA
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
QDVAQ
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 1644752; 10692383 [H]