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The map label for this gene is acrD [H]

Identifier: 157371729

GI number: 157371729

Start: 3865560

End: 3867641

Strand: Direct

Name: acrD [H]

Synonym: Spro_3492

Alternate gene names: 157371729

Gene position: 3865560-3867641 (Clockwise)

Preceding gene: 157371727

Following gene: 157371730

Centisome position: 70.94

GC content: 56.48

Gene sequence:

>2082_bases
ATGGCCAATTTTTTTATTGATCGCCCGATTTTCGCCTGGGTCCTGGCAATTATTCTTTGCCTGACCGGCGCTTTGGCGAT
TTTTTCCTTACCGGTGGAACAATACCCGAATCTGGCGCCGCCGAACGTTCGCATTAGCGCCACCTATCCAGGTGCTTCGG
CACAGACGCTGGAAAACACCGTCACCCAGATTATCGAACAGAACATGACGGGTCTGGACAACATGATGTATATGTCGTCG
CAGAGCACCAACACCGGGCGAGCCACGGTAACGCTGACCTTTGAAGCCGGTACCGACCCCAACGAGGCGATGCAGCAGGT
ACAAAACCAACTGCAGGCGGCGATTAAGCGACTGCCGCAGGCCGTGCAGACCCAGGGCGTAACGGTATCGAAATCCGGTG
ATACCACGCTGATGATGGTGGCCTTCGTTTCCACCGACGGCAGCATGGACAAGCAGGACATTGCCGATTACATCGTTTCC
AATCTGCAGGATCCGCTCAGCCGCATCAACGGCGTGGGCAGTATGGACGTTTACGGTTCGCAATACGCCATGCGCATCTG
GCTCGATCCCAACAAACTTAACAGTTACCAGCTCACCACCCAGGACGTCGTCAGCGCGATCCAATCGCAGAACGCCCAGG
TCGCCGTCGGCCAGCTCGGCGGTACACCCTCCGTCGATAAACAGGCGCTTAACGCCACCATCAATGCACAGTCTCAGTTG
CAAACGCCTGAACAATTCCAAAAAATTACCCTGCGAGTCAACCAGGACGGTTCGTTAGTCACGCTTGGCGATGTTGCCAC
CATCGAACTCGGTGGCGAAAACTATGACTATCTCAGCCGCTATAACGGCATGCAGGCCGCCGGCATGAACATCAAGCTGG
CCTCCGGTGCCAACGAGTTGCAAACCGATCAGTTGGTGAAGGATAAAATCGCCGAGCTGTCGCAGTATTTCCCGCATGGG
CTGGAGGCGAAGGTCGCCTATGAAACCACCCCGTTCGTTAAGGCCTCAATCAAAGACGTGGTAAAAACCCTGCTGGAAGC
CATTGTGCTGGTGTTCCTGGTGATGTACCTGTTCCTGCAGAACTTCCGCGCCACGCTGATCCCGACCATCGCCGTGCCGG
TAGTGTTGATGGGCACCTTTGCCATTCTTTCGGCCTGCGGATTCAGTATCAACACCCTGACCATGTTCGCCATGGTGCTG
GCCATCGGGCTATTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGCGTGATGAGTGAAGAAGGCCTGTCGCC
CAGGGATGCCACTCGCAAATCCATGGGGCAGATCCAGAGTGCGCTGGTCGGCATCGCCATGGTGTTGTCGGCGGTGTTTA
TCCCAATGGCGTTCTTCGGCGGTACCACCGGCGCCATTTACCGCCAGTTCTCGATCACCATTGTTTCTGCCATGGTACTG
TCGGTGCTGGTGGCGATGATCCTCACCCCTGCCCTGTGCGCCACCCTGCTCAAACCCATTGCCAAAGGCCACCATCACGG
CAAGCGCGGCTTCTTCGGCTGGTTCAACCGCATGTTCAACCGCAACGCCGACCGCTACGAGCGCGGCGTGGCGCGGGTGC
TGCACTCCAGTGTGCGTTATATAGTGATTTACCTGTTACTGCTCGGCGGCATGGCGTTACTGTTTATCAAGTTGCCGACC
TCCTTCCTGCCGCAGGAAGACCGTGGCGTATTCACCGTCCAGGTGCAATTGCCGCCGGGTTCAACCCTGCAGCAGACCAC
CAAAGTGGTGGAAAAAGTTGAACACTATTTCCTGACCAAAGAAAAACAGGACGTGCTGTCAGTGTTCTCCACCATTGGCG
CCGGCCCCGGCGGGAACGGGCAGAACGTGGCACGCATGTTTATTCGCTTGAAAGACTGGGATTTGCGTACCTCCGGTGCC
AACAGCTCATTCGATATCATCGATCGCGCCACCAAGGAATTTAACCAAATTATCGAGGCGCGGGTGATTGCCAGCAGTCC
ACCGGCGATTACCGGTCTGGCAATTCTTCCGGCTTCGATATGGAACTCGAAGACCACGCCGGCCTGGGCCACGACAAACT
GA

Upstream 100 bases:

>100_bases
GCGTAAAGTGCATAAGTGCCGGAATCCTTCCCAGCCACTGAGAAAACGCAATTCCGATGCGTCTTAAAGATAGAACAAGT
CATAACGAGGTTCGCGCTGC

Downstream 100 bases:

>100_bases
TGGCGGCACGCAATCAACTGCTGCAACTGGCCGCCGCTAACCCGCTGCTGTCCCGCGTGCGCCACAATGGCCTGGATGAC
AGCCCGCAGCTGCAAATTGA

Product: hydrophobe/amphiphile efflux-1 (HAE1) family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Acriflavine resistance protein D [H]

Number of amino acids: Translated: 693; Mature: 692

Protein sequence:

>693_residues
MANFFIDRPIFAWVLAIILCLTGALAIFSLPVEQYPNLAPPNVRISATYPGASAQTLENTVTQIIEQNMTGLDNMMYMSS
QSTNTGRATVTLTFEAGTDPNEAMQQVQNQLQAAIKRLPQAVQTQGVTVSKSGDTTLMMVAFVSTDGSMDKQDIADYIVS
NLQDPLSRINGVGSMDVYGSQYAMRIWLDPNKLNSYQLTTQDVVSAIQSQNAQVAVGQLGGTPSVDKQALNATINAQSQL
QTPEQFQKITLRVNQDGSLVTLGDVATIELGGENYDYLSRYNGMQAAGMNIKLASGANELQTDQLVKDKIAELSQYFPHG
LEAKVAYETTPFVKASIKDVVKTLLEAIVLVFLVMYLFLQNFRATLIPTIAVPVVLMGTFAILSACGFSINTLTMFAMVL
AIGLLVDDAIVVVENVERVMSEEGLSPRDATRKSMGQIQSALVGIAMVLSAVFIPMAFFGGTTGAIYRQFSITIVSAMVL
SVLVAMILTPALCATLLKPIAKGHHHGKRGFFGWFNRMFNRNADRYERGVARVLHSSVRYIVIYLLLLGGMALLFIKLPT
SFLPQEDRGVFTVQVQLPPGSTLQQTTKVVEKVEHYFLTKEKQDVLSVFSTIGAGPGGNGQNVARMFIRLKDWDLRTSGA
NSSFDIIDRATKEFNQIIEARVIASSPPAITGLAILPASIWNSKTTPAWATTN

Sequences:

>Translated_693_residues
MANFFIDRPIFAWVLAIILCLTGALAIFSLPVEQYPNLAPPNVRISATYPGASAQTLENTVTQIIEQNMTGLDNMMYMSS
QSTNTGRATVTLTFEAGTDPNEAMQQVQNQLQAAIKRLPQAVQTQGVTVSKSGDTTLMMVAFVSTDGSMDKQDIADYIVS
NLQDPLSRINGVGSMDVYGSQYAMRIWLDPNKLNSYQLTTQDVVSAIQSQNAQVAVGQLGGTPSVDKQALNATINAQSQL
QTPEQFQKITLRVNQDGSLVTLGDVATIELGGENYDYLSRYNGMQAAGMNIKLASGANELQTDQLVKDKIAELSQYFPHG
LEAKVAYETTPFVKASIKDVVKTLLEAIVLVFLVMYLFLQNFRATLIPTIAVPVVLMGTFAILSACGFSINTLTMFAMVL
AIGLLVDDAIVVVENVERVMSEEGLSPRDATRKSMGQIQSALVGIAMVLSAVFIPMAFFGGTTGAIYRQFSITIVSAMVL
SVLVAMILTPALCATLLKPIAKGHHHGKRGFFGWFNRMFNRNADRYERGVARVLHSSVRYIVIYLLLLGGMALLFIKLPT
SFLPQEDRGVFTVQVQLPPGSTLQQTTKVVEKVEHYFLTKEKQDVLSVFSTIGAGPGGNGQNVARMFIRLKDWDLRTSGA
NSSFDIIDRATKEFNQIIEARVIASSPPAITGLAILPASIWNSKTTPAWATTN
>Mature_692_residues
ANFFIDRPIFAWVLAIILCLTGALAIFSLPVEQYPNLAPPNVRISATYPGASAQTLENTVTQIIEQNMTGLDNMMYMSSQ
STNTGRATVTLTFEAGTDPNEAMQQVQNQLQAAIKRLPQAVQTQGVTVSKSGDTTLMMVAFVSTDGSMDKQDIADYIVSN
LQDPLSRINGVGSMDVYGSQYAMRIWLDPNKLNSYQLTTQDVVSAIQSQNAQVAVGQLGGTPSVDKQALNATINAQSQLQ
TPEQFQKITLRVNQDGSLVTLGDVATIELGGENYDYLSRYNGMQAAGMNIKLASGANELQTDQLVKDKIAELSQYFPHGL
EAKVAYETTPFVKASIKDVVKTLLEAIVLVFLVMYLFLQNFRATLIPTIAVPVVLMGTFAILSACGFSINTLTMFAMVLA
IGLLVDDAIVVVENVERVMSEEGLSPRDATRKSMGQIQSALVGIAMVLSAVFIPMAFFGGTTGAIYRQFSITIVSAMVLS
VLVAMILTPALCATLLKPIAKGHHHGKRGFFGWFNRMFNRNADRYERGVARVLHSSVRYIVIYLLLLGGMALLFIKLPTS
FLPQEDRGVFTVQVQLPPGSTLQQTTKVVEKVEHYFLTKEKQDVLSVFSTIGAGPGGNGQNVARMFIRLKDWDLRTSGAN
SSFDIIDRATKEFNQIIEARVIASSPPAITGLAILPASIWNSKTTPAWATTN

Specific function: Participates in the efflux of aminoglycosides. Confers resistance to a variety of these substances [H]

COG id: COG0841

COG function: function code V; Cation/multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AcrB/AcrD/AcrF (TC 2.A.6) family [H]

Homologues:

Organism=Escherichia coli, GI1788814, Length=674, Percent_Identity=79.3768545994065, Blast_Score=1137, Evalue=0.0,
Organism=Escherichia coli, GI1786667, Length=675, Percent_Identity=64, Blast_Score=884, Evalue=0.0,
Organism=Escherichia coli, GI1789666, Length=674, Percent_Identity=60.9792284866469, Blast_Score=844, Evalue=0.0,
Organism=Escherichia coli, GI1789930, Length=673, Percent_Identity=59.5839524517088, Blast_Score=800, Evalue=0.0,
Organism=Escherichia coli, GI1788390, Length=637, Percent_Identity=32.182103610675, Blast_Score=301, Evalue=1e-82,
Organism=Escherichia coli, GI1788391, Length=650, Percent_Identity=30.3076923076923, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1786788, Length=623, Percent_Identity=25.8426966292135, Blast_Score=178, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001036
- InterPro:   IPR004764 [H]

Pfam domain/function: PF00873 ACR_tran [H]

EC number: NA

Molecular weight: Translated: 75440; Mature: 75309

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANFFIDRPIFAWVLAIILCLTGALAIFSLPVEQYPNLAPPNVRISATYPGASAQTLENT
CCCCEECCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEEEECCCCCHHHHHHH
VTQIIEQNMTGLDNMMYMSSQSTNTGRATVTLTFEAGTDPNEAMQQVQNQLQAAIKRLPQ
HHHHHHHHCCCHHHHHEECCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
AVQTQGVTVSKSGDTTLMMVAFVSTDGSMDKQDIADYIVSNLQDPLSRINGVGSMDVYGS
HHHHCCEEEECCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
QYAMRIWLDPNKLNSYQLTTQDVVSAIQSQNAQVAVGQLGGTPSVDKQALNATINAQSQL
CEEEEEEECCCCCCCEECCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHC
QTPEQFQKITLRVNQDGSLVTLGDVATIELGGENYDYLSRYNGMQAAGMNIKLASGANEL
CCHHHHHEEEEEECCCCCEEEECCEEEEEECCCCCHHHHHCCCCEECCCEEEECCCCCHH
QTDQLVKDKIAELSQYFPHGLEAKVAYETTPFVKASIKDVVKTLLEAIVLVFLVMYLFLQ
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFRATLIPTIAVPVVLMGTFAILSACGFSINTLTMFAMVLAIGLLVDDAIVVVENVERVM
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEEGLSPRDATRKSMGQIQSALVGIAMVLSAVFIPMAFFGGTTGAIYRQFSITIVSAMVL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SVLVAMILTPALCATLLKPIAKGHHHGKRGFFGWFNRMFNRNADRYERGVARVLHSSVRY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IVIYLLLLGGMALLFIKLPTSFLPQEDRGVFTVQVQLPPGSTLQQTTKVVEKVEHYFLTK
HHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCC
EKQDVLSVFSTIGAGPGGNGQNVARMFIRLKDWDLRTSGANSSFDIIDRATKEFNQIIEA
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RVIASSPPAITGLAILPASIWNSKTTPAWATTN
HHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
ANFFIDRPIFAWVLAIILCLTGALAIFSLPVEQYPNLAPPNVRISATYPGASAQTLENT
CCCEECCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEEEECCCCCHHHHHHH
VTQIIEQNMTGLDNMMYMSSQSTNTGRATVTLTFEAGTDPNEAMQQVQNQLQAAIKRLPQ
HHHHHHHHCCCHHHHHEECCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
AVQTQGVTVSKSGDTTLMMVAFVSTDGSMDKQDIADYIVSNLQDPLSRINGVGSMDVYGS
HHHHCCEEEECCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
QYAMRIWLDPNKLNSYQLTTQDVVSAIQSQNAQVAVGQLGGTPSVDKQALNATINAQSQL
CEEEEEEECCCCCCCEECCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHC
QTPEQFQKITLRVNQDGSLVTLGDVATIELGGENYDYLSRYNGMQAAGMNIKLASGANEL
CCHHHHHEEEEEECCCCCEEEECCEEEEEECCCCCHHHHHCCCCEECCCEEEECCCCCHH
QTDQLVKDKIAELSQYFPHGLEAKVAYETTPFVKASIKDVVKTLLEAIVLVFLVMYLFLQ
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFRATLIPTIAVPVVLMGTFAILSACGFSINTLTMFAMVLAIGLLVDDAIVVVENVERVM
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEEGLSPRDATRKSMGQIQSALVGIAMVLSAVFIPMAFFGGTTGAIYRQFSITIVSAMVL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SVLVAMILTPALCATLLKPIAKGHHHGKRGFFGWFNRMFNRNADRYERGVARVLHSSVRY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IVIYLLLLGGMALLFIKLPTSFLPQEDRGVFTVQVQLPPGSTLQQTTKVVEKVEHYFLTK
HHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCC
EKQDVLSVFSTIGAGPGGNGQNVARMFIRLKDWDLRTSGANSSFDIIDRATKEFNQIIEA
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RVIASSPPAITGLAILPASIWNSKTTPAWATTN
HHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 1644752; 10692383 [H]