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The map label for this gene is narQ [H]

Identifier: 157371726

GI number: 157371726

Start: 3862495

End: 3864177

Strand: Direct

Name: narQ [H]

Synonym: Spro_3489

Alternate gene names: 157371726

Gene position: 3862495-3864177 (Clockwise)

Preceding gene: 157371719

Following gene: 157371727

Centisome position: 70.89

GC content: 57.87

Gene sequence:

>1683_bases
TTGCTTGTTAAACGTTCAGTGACCAGCAGCCTGGCCAAGGCGCTGTTTTGCATTGTGATTTTGTCCGTGCTCTCTACCGG
GCTGGCGCTAACCACCGTGGCTGACAGCCTGCGTGACGCCGAAGCGGTCAACATTGCCGGTTCGCTGCGCATGCAGAGCT
ACCGGCTGGCCTACGATCTCACTGGCCCGGCTCCGGAGCTGGAACAGCATCTATTACAATATCGGCAATCCCTGCAGGCC
CCGGCGTTGCAGAAGTTGGATCGTTTTTACGTGCCAGCGGAAGTGCGGGAAAAGTACCTGTCTTTGCAGCATGCCTGGCA
ACCCTTGGCGCAGCAGATCCAGGCCGGCCAGTCGGCCGACTATCAGGCCAATATCGCCGGCTACGTTAACCAGATTGACC
ATTTCGTGTTGGCGCTACAGCGTTATTCCGAGCTGAAACTGACTATTGTGGCGGCCATCAGCGTGCTGGGTTTTATCGCC
ATCATCGGCCTGGTGCTGTTTTGCATCCGTTTTATGCGCCGTCAGGTGGTGGCACCGCTGAAGCATCTGGTGGATGCCAG
CCAGCGCGTGCAACAGCGGGACTTTAACCACCCACCGCTGGATACCGCCCTGCCGAATGAGCTGGGCGTTTTGTCGCAGG
CCTTTACCGCCATGTCGGGCGACCTGGCCAAGTTGTATGAGTCGCTGGAGCAGAAAGTGCAGGAGAAAACCCAGCGTCTG
CAACAGGCGAACAAGACGCTGGAGGTGCTGTACAACTGCTCGCAGGCACTTAACGTGCGGCAGATAGACCAGCAGGCATT
TGAGAAGATCCTGCACATCGTGCGCCAAAGTGAAGATTTGCACTGCATCCGGCTAAACGTGGCGGACAGCCTCAGCGAAT
GGCAATTGCGCAGCGGCCGCCCTTCGCCGACGCTGGCCTGGCAATCGCTGACCATTATCCAGGGCGACAAACCGCTGGGT
GAACTGAGTTGGCAGCATAAGGAACAGCCACCACATCCGCACCTGATGCAGAGCGTGGCCAATATGCTGAGTCGTGGGGT
TTATTTTAACCGCGCGCAGAAACAGCATATGCAATTCTTGCTGATGGAAGAACGTGCCACCATCGCCCGCGAGCTGCACG
ATTCTCTGGCACAGGCGCTGTCGTTCTTGCGTATTCAGTTAACCCTGCTCAGGCGCACGCTGGCGGACAGCACCCCTGAA
GCCCAAGAGATTATTAGCGATTTTGACCAAGCGCTGGCCGACGCCTATCGCCAACTGCGCGAACTGCTGACCACCTTCCG
CCTGAACATTCAGGAGGCCGATTTGAACACCGCGCTGCACCAGTTGCTGGCGCCATTAAAGGTTCTTACTGACGCACGGA
TTCAGTTACACTGTCGGCTTTCTTCCCAGGCGCTCAATGCCCAGCAGCAGGTGCATGCGCTACAGATTGTGCGCGAAGCG
GTGCTTAACGCCATCAAACATGCCCAGGCACAGGAAATCGTCATTCGCTGCGAAATGACGGCCGAGGGTGATAACAGCAT
CAGCATCGCTGACGACGGTTGCGGCATCGCCAGCCTGAATGAACCTGAAGGTCATTATGGTCTGACCATCATGAGCGAGC
GCGCTGCGCGACTGGGAGGAACGCTGCGCATCCAGCGGGGTGAGCCGGACGGCACGGAAGTCCGCCTGACATTCCCGCCA
TAA

Upstream 100 bases:

>100_bases
ATTTCATGCAAAACGCTACGCATTTTAGTTGCAAAACTTATAATTTTGCTTTCGAATGAAACCCGGTGATGCTCATCGCC
CGCGAGAAAAGGATGCATGA

Downstream 100 bases:

>100_bases
AATCAGGACTGTTCACTCTTCGTTACGGTTTAGCTGAGTCTAACGGTCTATTTCAGGATGTTCGTGGCGCCAATCGCCAC
TATTTTATAAGAGTCTGTCC

Product: nitrate/nitrite sensor protein NarQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MLVKRSVTSSLAKALFCIVILSVLSTGLALTTVADSLRDAEAVNIAGSLRMQSYRLAYDLTGPAPELEQHLLQYRQSLQA
PALQKLDRFYVPAEVREKYLSLQHAWQPLAQQIQAGQSADYQANIAGYVNQIDHFVLALQRYSELKLTIVAAISVLGFIA
IIGLVLFCIRFMRRQVVAPLKHLVDASQRVQQRDFNHPPLDTALPNELGVLSQAFTAMSGDLAKLYESLEQKVQEKTQRL
QQANKTLEVLYNCSQALNVRQIDQQAFEKILHIVRQSEDLHCIRLNVADSLSEWQLRSGRPSPTLAWQSLTIIQGDKPLG
ELSWQHKEQPPHPHLMQSVANMLSRGVYFNRAQKQHMQFLLMEERATIARELHDSLAQALSFLRIQLTLLRRTLADSTPE
AQEIISDFDQALADAYRQLRELLTTFRLNIQEADLNTALHQLLAPLKVLTDARIQLHCRLSSQALNAQQQVHALQIVREA
VLNAIKHAQAQEIVIRCEMTAEGDNSISIADDGCGIASLNEPEGHYGLTIMSERAARLGGTLRIQRGEPDGTEVRLTFPP

Sequences:

>Translated_560_residues
MLVKRSVTSSLAKALFCIVILSVLSTGLALTTVADSLRDAEAVNIAGSLRMQSYRLAYDLTGPAPELEQHLLQYRQSLQA
PALQKLDRFYVPAEVREKYLSLQHAWQPLAQQIQAGQSADYQANIAGYVNQIDHFVLALQRYSELKLTIVAAISVLGFIA
IIGLVLFCIRFMRRQVVAPLKHLVDASQRVQQRDFNHPPLDTALPNELGVLSQAFTAMSGDLAKLYESLEQKVQEKTQRL
QQANKTLEVLYNCSQALNVRQIDQQAFEKILHIVRQSEDLHCIRLNVADSLSEWQLRSGRPSPTLAWQSLTIIQGDKPLG
ELSWQHKEQPPHPHLMQSVANMLSRGVYFNRAQKQHMQFLLMEERATIARELHDSLAQALSFLRIQLTLLRRTLADSTPE
AQEIISDFDQALADAYRQLRELLTTFRLNIQEADLNTALHQLLAPLKVLTDARIQLHCRLSSQALNAQQQVHALQIVREA
VLNAIKHAQAQEIVIRCEMTAEGDNSISIADDGCGIASLNEPEGHYGLTIMSERAARLGGTLRIQRGEPDGTEVRLTFPP
>Mature_560_residues
MLVKRSVTSSLAKALFCIVILSVLSTGLALTTVADSLRDAEAVNIAGSLRMQSYRLAYDLTGPAPELEQHLLQYRQSLQA
PALQKLDRFYVPAEVREKYLSLQHAWQPLAQQIQAGQSADYQANIAGYVNQIDHFVLALQRYSELKLTIVAAISVLGFIA
IIGLVLFCIRFMRRQVVAPLKHLVDASQRVQQRDFNHPPLDTALPNELGVLSQAFTAMSGDLAKLYESLEQKVQEKTQRL
QQANKTLEVLYNCSQALNVRQIDQQAFEKILHIVRQSEDLHCIRLNVADSLSEWQLRSGRPSPTLAWQSLTIIQGDKPLG
ELSWQHKEQPPHPHLMQSVANMLSRGVYFNRAQKQHMQFLLMEERATIARELHDSLAQALSFLRIQLTLLRRTLADSTPE
AQEIISDFDQALADAYRQLRELLTTFRLNIQEADLNTALHQLLAPLKVLTDARIQLHCRLSSQALNAQQQVHALQIVREA
VLNAIKHAQAQEIVIRCEMTAEGDNSISIADDGCGIASLNEPEGHYGLTIMSERAARLGGTLRIQRGEPDGTEVRLTFPP

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate/nitrite availability to the narL/narP proteins. NarQ probably activates narL and narP by phosphorylation. NarQ probably negatively regulates the narL protein by dephosphorylation [H]

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=558, Percent_Identity=54.4802867383513, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI1787474, Length=604, Percent_Identity=30.4635761589404, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87082311, Length=215, Percent_Identity=29.7674418604651, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 62835; Mature: 62835

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEECCCCCCCEEEEECCC
>Mature Secondary Structure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EEEEECCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1528845; 1508040; 9205837; 9278503 [H]