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The map label for this gene is rob [H]

Identifier: 157371719

GI number: 157371719

Start: 3856396

End: 3856818

Strand: Direct

Name: rob [H]

Synonym: Spro_3482

Alternate gene names: 157371719

Gene position: 3856396-3856818 (Clockwise)

Preceding gene: 157371718

Following gene: 157371726

Centisome position: 70.77

GC content: 43.74

Gene sequence:

>423_bases
ATGGCAATTAATATGAAGATTTTTATTAATGAGATGCTTAAATGGATAGAGGGCAATTTGGGAGCCGAGCTGAATATAAA
GACCATTGCAAAAAAATCCGGCTACAGCAGATTCCACATTCAACGGCTTTTCAAGTTGCATGCAGGGCTAACGCTTGCCC
AATATATCAGAATGCGGCGCGTCACTGAGGCGGCGATCGCACTGAGATTTACCGACAGAAAAATCATGGATATCGCACTG
GATTATGGCTTTGACTCTCAACAGGTTTTTACTCGGACATTCAAAAAACAGTTAAAAACCACGCCAGGCGAGCTGAGGAG
AAGCAAGCAATTAAACAATAACCGGCTCTGTTACTCATTGTACATGCCGACGATGGTGCAAAAACCCTCGACAATCCAGC
CTAACGCCCACAAACCTAACTAA

Upstream 100 bases:

>100_bases
CCCGTACACCGCCAGCATTCTTTTTATAAATCAGAGGTCATTCACCCAAACGACAGAACATGAACGCCCCCAAAAGTCTA
TGCGATAAAAGGAGTTTTCC

Downstream 100 bases:

>100_bases
CTCACGTTGGATTCATCTCGCCGTTCGCCGGCGAGATGAATAGTCTGTCTCAGTCCATAATGCGGTGAATTTGCAGGTAC
TGCAGCAGCATAATAGTCTT

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 140; Mature: 139

Protein sequence:

>140_residues
MAINMKIFINEMLKWIEGNLGAELNIKTIAKKSGYSRFHIQRLFKLHAGLTLAQYIRMRRVTEAAIALRFTDRKIMDIAL
DYGFDSQQVFTRTFKKQLKTTPGELRRSKQLNNNRLCYSLYMPTMVQKPSTIQPNAHKPN

Sequences:

>Translated_140_residues
MAINMKIFINEMLKWIEGNLGAELNIKTIAKKSGYSRFHIQRLFKLHAGLTLAQYIRMRRVTEAAIALRFTDRKIMDIAL
DYGFDSQQVFTRTFKKQLKTTPGELRRSKQLNNNRLCYSLYMPTMVQKPSTIQPNAHKPN
>Mature_139_residues
AINMKIFINEMLKWIEGNLGAELNIKTIAKKSGYSRFHIQRLFKLHAGLTLAQYIRMRRVTEAAIALRFTDRKIMDIALD
YGFDSQQVFTRTFKKQLKTTPGELRRSKQLNNNRLCYSLYMPTMVQKPSTIQPNAHKPN

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=104, Percent_Identity=50, Blast_Score=114, Evalue=3e-27,
Organism=Escherichia coli, GI1790497, Length=98, Percent_Identity=45.9183673469388, Blast_Score=101, Evalue=2e-23,
Organism=Escherichia coli, GI87081928, Length=114, Percent_Identity=40.3508771929825, Blast_Score=99, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 16338; Mature: 16206

Theoretical pI: Translated: 11.03; Mature: 11.03

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAINMKIFINEMLKWIEGNLGAELNIKTIAKKSGYSRFHIQRLFKLHAGLTLAQYIRMRR
CEEEHHHHHHHHHHHHCCCCCCEEEHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
VTEAAIALRFTDRKIMDIALDYGFDSQQVFTRTFKKQLKTTPGELRRSKQLNNNRLCYSL
HHHHHHHHHCCCCCEEEHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEE
YMPTMVQKPSTIQPNAHKPN
ECCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AINMKIFINEMLKWIEGNLGAELNIKTIAKKSGYSRFHIQRLFKLHAGLTLAQYIRMRR
EEEHHHHHHHHHHHHCCCCCCEEEHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
VTEAAIALRFTDRKIMDIALDYGFDSQQVFTRTFKKQLKTTPGELRRSKQLNNNRLCYSL
HHHHHHHHHCCCCCEEEHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEE
YMPTMVQKPSTIQPNAHKPN
ECCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]