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The map label for this gene is oppA [H]

Identifier: 157370942

GI number: 157370942

Start: 2975119

End: 2976756

Strand: Reverse

Name: oppA [H]

Synonym: Spro_2702

Alternate gene names: 157370942

Gene position: 2976756-2975119 (Counterclockwise)

Preceding gene: 157370943

Following gene: 157370941

Centisome position: 54.63

GC content: 54.09

Gene sequence:

>1638_bases
ATGCAGCAAACCCTAAAGCACACACCATTAGCTATGTTGATTGCCGGCCTGCTGAGTATCAGCGCCGTTTATTCTGCTCA
GGCGGCTGATGTTCCGGCCGGGGTGCAATTGGCCCAGCAGCAGAAGATCGTCATTAACAACGGCTCGGAAGTGGCTTCAT
TGGATCCGCACAAGGTTGAAGGCATACCGGAAAGCAATATCATTCTGAACCTGCTGGAAGGGCTGGTCAGCACCGACGCC
AATGGGCACGTGGTCGCGGCTGCGGCGACAAATTGGGAAAATCAGGATTTTAAGACCTGGACTTTCCACCTGCGTCCCGA
CGCAGTTTGGAGTGACGGCTCCCCGGTTACCGCGCAGGATTTTGTCTACAGTTGGCAACGCCTGGCCGACCCGAAAATTG
GCTCACCTTATGCCAGCTACCTGCAGTACGCCAAAGTTGAAAATATCGACGCCATTCTGACCGGCAAGCAAAGCCCGCAG
ACGCTCGGTATTAAAGCTATCGATGATAAAACTTTTCAGGTCACGCTTAGCGAGCCGGTGCCGTATTTTATCAGCATGCT
GAGCCACACCTCGCTGAAACCGGTAAAACGTTCGGTAGTTGAGAAGTTTGGCGACAAGTGGACGCTGCCGGCAAACTATG
TCGGTAATGGCGCGTATCGCTTGAAAGATTGGGTGGTGAATGAGCGCATTGTGCTTGAACGCAGCCCGAGCTACTGGAAC
AACAAGAAAACGGTTATCAGCCAGGCAACCTTCTTGCCGATATCTTCAGAGGTCAGTGACGTCAATCGCTTCCGCAGCGG
TGAAATCGATATCACCAACAGCGCCATCCCGCCGAACCTGTTCGCGAAAATGAAAAGCGAGATCCCTGAGCAATTGCACG
TTAACCCTTATCTGTGCACTTTTTATTACGAGATTAACAACCAGCGCGCGCCTTTTACCGATGCCCGGGTGCGAGCCGCA
GTGAAACTGACGTTGGATCGCGACATCATTGCCAACAAGATCATGGGCCAGGGGCAGATCCCGGCCTACAGCTTTACTCC
GAGCTTTACCGAGGGTGCCAAATTCACGGCGCCGGAATGGGCAGGGTGGAGCCAGGAGCAACGTAATGCCGAGGCGAAGA
AACTGTTGGCCGAGGCAGGCTTCAGCGCGACCAAACCGCTGAAATTCACATTGCTGTACAACACTTCAGATCAAAATAAG
CAGCAGGCGATCGCTGCCGCGTCAATGTGGAAGAAAAACCTCGGTGCGGATGTCCAGCTGCGCAATCAGGAGTGGAAAAC
CTCGCTGGAAAGCCGCCATCAGGGGCAGTACGACGTGGCACGGGCCACCTGGTGCGGAGACTATAACGAGCCTAGCGCTT
TCCTGAATCTGGTTGTCTCCAATAGCAGCATCAATACCATTTTTTACAAGAGCCCGGCGTTTGACGCGCTGATGGCCAGC
ACGCTAAAAGCGCCGGACGAAGCTGCACGGGCTGCTCTTTATCAGCAAGCGGAGGCTCAGTTGGACAAAGATTCGGCGTT
GATCCCGGTGTATTACCGCGTCAGTGCGCGCCTGATCAGACCGTCGGTGGGGGGCTTTACCGGTAAAGACCCGCTGGATT
ACACCGATGTGAAAAACCTGTACATCATCAAGCAGTAA

Upstream 100 bases:

>100_bases
AGTGGGAATGATTTTTGCTGGTGTCTGTTTATTTAGAATTCTTATAAATACTTCACGAATATCGATAAGCAATGCTTGTT
GGTGAAAAGGAGCCTCGACC

Downstream 100 bases:

>100_bases
CGAGGGGTATTACGCCACGCCAACAGGGGTGAGTAATACAAGGGAGTCCGCCTGCCGTATTCGTTCAACGGCCGTCGGCA
TAACAAGGTCCGCTCACGGG

Product: extracellular solute-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MQQTLKHTPLAMLIAGLLSISAVYSAQAADVPAGVQLAQQQKIVINNGSEVASLDPHKVEGIPESNIILNLLEGLVSTDA
NGHVVAAAATNWENQDFKTWTFHLRPDAVWSDGSPVTAQDFVYSWQRLADPKIGSPYASYLQYAKVENIDAILTGKQSPQ
TLGIKAIDDKTFQVTLSEPVPYFISMLSHTSLKPVKRSVVEKFGDKWTLPANYVGNGAYRLKDWVVNERIVLERSPSYWN
NKKTVISQATFLPISSEVSDVNRFRSGEIDITNSAIPPNLFAKMKSEIPEQLHVNPYLCTFYYEINNQRAPFTDARVRAA
VKLTLDRDIIANKIMGQGQIPAYSFTPSFTEGAKFTAPEWAGWSQEQRNAEAKKLLAEAGFSATKPLKFTLLYNTSDQNK
QQAIAAASMWKKNLGADVQLRNQEWKTSLESRHQGQYDVARATWCGDYNEPSAFLNLVVSNSSINTIFYKSPAFDALMAS
TLKAPDEAARAALYQQAEAQLDKDSALIPVYYRVSARLIRPSVGGFTGKDPLDYTDVKNLYIIKQ

Sequences:

>Translated_545_residues
MQQTLKHTPLAMLIAGLLSISAVYSAQAADVPAGVQLAQQQKIVINNGSEVASLDPHKVEGIPESNIILNLLEGLVSTDA
NGHVVAAAATNWENQDFKTWTFHLRPDAVWSDGSPVTAQDFVYSWQRLADPKIGSPYASYLQYAKVENIDAILTGKQSPQ
TLGIKAIDDKTFQVTLSEPVPYFISMLSHTSLKPVKRSVVEKFGDKWTLPANYVGNGAYRLKDWVVNERIVLERSPSYWN
NKKTVISQATFLPISSEVSDVNRFRSGEIDITNSAIPPNLFAKMKSEIPEQLHVNPYLCTFYYEINNQRAPFTDARVRAA
VKLTLDRDIIANKIMGQGQIPAYSFTPSFTEGAKFTAPEWAGWSQEQRNAEAKKLLAEAGFSATKPLKFTLLYNTSDQNK
QQAIAAASMWKKNLGADVQLRNQEWKTSLESRHQGQYDVARATWCGDYNEPSAFLNLVVSNSSINTIFYKSPAFDALMAS
TLKAPDEAARAALYQQAEAQLDKDSALIPVYYRVSARLIRPSVGGFTGKDPLDYTDVKNLYIIKQ
>Mature_545_residues
MQQTLKHTPLAMLIAGLLSISAVYSAQAADVPAGVQLAQQQKIVINNGSEVASLDPHKVEGIPESNIILNLLEGLVSTDA
NGHVVAAAATNWENQDFKTWTFHLRPDAVWSDGSPVTAQDFVYSWQRLADPKIGSPYASYLQYAKVENIDAILTGKQSPQ
TLGIKAIDDKTFQVTLSEPVPYFISMLSHTSLKPVKRSVVEKFGDKWTLPANYVGNGAYRLKDWVVNERIVLERSPSYWN
NKKTVISQATFLPISSEVSDVNRFRSGEIDITNSAIPPNLFAKMKSEIPEQLHVNPYLCTFYYEINNQRAPFTDARVRAA
VKLTLDRDIIANKIMGQGQIPAYSFTPSFTEGAKFTAPEWAGWSQEQRNAEAKKLLAEAGFSATKPLKFTLLYNTSDQNK
QQAIAAASMWKKNLGADVQLRNQEWKTSLESRHQGQYDVARATWCGDYNEPSAFLNLVVSNSSINTIFYKSPAFDALMAS
TLKAPDEAARAALYQQAEAQLDKDSALIPVYYRVSARLIRPSVGGFTGKDPLDYTDVKNLYIIKQ

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=539, Percent_Identity=64.9350649350649, Blast_Score=758, Evalue=0.0,
Organism=Escherichia coli, GI87081878, Length=545, Percent_Identity=44.5871559633028, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1789397, Length=532, Percent_Identity=42.6691729323308, Blast_Score=427, Evalue=1e-120,
Organism=Escherichia coli, GI1787762, Length=458, Percent_Identity=26.8558951965066, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1789887, Length=481, Percent_Identity=23.4927234927235, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789966, Length=489, Percent_Identity=23.1083844580777, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1787052, Length=502, Percent_Identity=22.1115537848606, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787551, Length=555, Percent_Identity=20.9009009009009, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60535; Mature: 60535

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQTLKHTPLAMLIAGLLSISAVYSAQAADVPAGVQLAQQQKIVINNGSEVASLDPHKVE
CCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEEEECCCCEECCCCHHCC
GIPESNIILNLLEGLVSTDANGHVVAAAATNWENQDFKTWTFHLRPDAVWSDGSPVTAQD
CCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEEECCCCEECCCCCCCHHH
FVYSWQRLADPKIGSPYASYLQYAKVENIDAILTGKQSPQTLGIKAIDDKTFQVTLSEPV
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCEECEEEECCCEEEEEECCCH
PYFISMLSHTSLKPVKRSVVEKFGDKWTLPANYVGNGAYRLKDWVVNERIVLERSPSYWN
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHEECCEEEEECCCCCCC
NKKTVISQATFLPISSEVSDVNRFRSGEIDITNSAIPPNLFAKMKSEIPEQLHVNPYLCT
CCEEEEEEEEEEECCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCHHHCCCEEEEE
FYYEINNQRAPFTDARVRAAVKLTLDRDIIANKIMGQGQIPAYSFTPSFTEGAKFTAPEW
EEEEECCCCCCCCHHHEEEEEEEEECHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCC
AGWSQEQRNAEAKKLLAEAGFSATKPLKFTLLYNTSDQNKQQAIAAASMWKKNLGADVQL
CCCCHHHHCHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEE
RNQEWKTSLESRHQGQYDVARATWCGDYNEPSAFLNLVVSNSSINTIFYKSPAFDALMAS
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCHHHHHHH
TLKAPDEAARAALYQQAEAQLDKDSALIPVYYRVSARLIRPSVGGFTGKDPLDYTDVKNL
HHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCE
YIIKQ
EEEEC
>Mature Secondary Structure
MQQTLKHTPLAMLIAGLLSISAVYSAQAADVPAGVQLAQQQKIVINNGSEVASLDPHKVE
CCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEEEECCCCEECCCCHHCC
GIPESNIILNLLEGLVSTDANGHVVAAAATNWENQDFKTWTFHLRPDAVWSDGSPVTAQD
CCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEEECCCCEECCCCCCCHHH
FVYSWQRLADPKIGSPYASYLQYAKVENIDAILTGKQSPQTLGIKAIDDKTFQVTLSEPV
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCEECEEEECCCEEEEEECCCH
PYFISMLSHTSLKPVKRSVVEKFGDKWTLPANYVGNGAYRLKDWVVNERIVLERSPSYWN
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHEECCEEEEECCCCCCC
NKKTVISQATFLPISSEVSDVNRFRSGEIDITNSAIPPNLFAKMKSEIPEQLHVNPYLCT
CCEEEEEEEEEEECCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCHHHCCCEEEEE
FYYEINNQRAPFTDARVRAAVKLTLDRDIIANKIMGQGQIPAYSFTPSFTEGAKFTAPEW
EEEEECCCCCCCCHHHEEEEEEEEECHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCC
AGWSQEQRNAEAKKLLAEAGFSATKPLKFTLLYNTSDQNKQQAIAAASMWKKNLGADVQL
CCCCHHHHCHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEE
RNQEWKTSLESRHQGQYDVARATWCGDYNEPSAFLNLVVSNSSINTIFYKSPAFDALMAS
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCHHHHHHH
TLKAPDEAARAALYQQAEAQLDKDSALIPVYYRVSARLIRPSVGGFTGKDPLDYTDVKNL
HHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCE
YIIKQ
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187863; 9097039; 9278503; 2015910; 9298646; 9600841 [H]