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The map label for this gene is oppA [H]

Identifier: 157370941

GI number: 157370941

Start: 2973329

End: 2974966

Strand: Reverse

Name: oppA [H]

Synonym: Spro_2701

Alternate gene names: 157370941

Gene position: 2974966-2973329 (Counterclockwise)

Preceding gene: 157370942

Following gene: 157370940

Centisome position: 54.6

GC content: 50.85

Gene sequence:

>1638_bases
ATGACCAACATCACGAAAAAAACCATCCTTGCGGCCGGCATCATTGCCGCGCTGGGTATCACAGCAACCGGAAGTGCTTT
TGCGGCCGTTGTGCCGGCGGGCGTACAACTGGCAGACAAGCAAGAGATAGTTAAAAACAACGGTTCGGAAGTACAGTCGC
TGGATCCGCACAAAATTGAAGGCGTACCTGAAAACAACGTTACGCGCGATCTGATGGAAGGCCTGGCCAACAACGGCCCG
GATGGTTCCATTGTTCCAGGCGTGGCCGAAAGCTGGGATAACAAAGATTTTAAAGTCTGGACCTTCCATCTGCGTAAAGA
CGCAAAGTGGTCGAATGGCAAGCCGGTAACGGCACAAGATTTCGTGTATAGCTGGCAGCGCGTGGTGGACCCTAAAACTG
CATCCCCATATGCCAGCTATCTGCAATATGCTCACGTAGAAAACGTTGATGACGTTATCGCCGGTAAAAAAGACAAGTCT
ACCCTGGGCGTAAAAGCCATCGACGATCACACCTTCCAGGTCACCCTGACCGAGCCGGTGCCTTACCTGGTTGAAATGAC
GCCGCACTATGCGATGAAGCCAGTATTTAAAGACGCGGTCGAGAAGTTTGGTGAGAAATGGACCCTGCCGGCGAATTACG
TCAGCAATGGTGCCTACAAGTTAAAAGACTGGGTCGTTAACGAACGTATCGTGCTGGAACGTAACCCGGAATATTGGGAT
AACGCGAAAACCATCATTAATAAAGTCACGTTCCTGCCAATTTCGTCGGAAGTGACCGACGTAAACCGCTACCGCACCGG
TGAAATCGACATGACCTATAACAACATGCCGATTGAACTGTTCCAAAAGTTGAAAAAAGAGATCCCGAAAGAAGTTCACG
TCGATCCATATCTGTGTACTTATTACTACGAGATCAACAACCAGAAAGCGCCGTTTACCGATCAGCGCGTACGTGAAGCG
CTGAAGCTGGGCCTGGATCGCGACATCATCACCAATAAAGTGAAAAACCAGGGTGATTTGCCGGCCTACAGCTTCACCCC
ACCTTATACCGATGGTGCCAAGTTGACGCCACCAGAGTGGTTCGGCTGGACGCAGGAAAAACGCAACGAAGTGGCGAAAA
AACTGCTGGCTGAAGCGGGTTACGGCCCGGGCAAACCACTGACTTTCTCACTGCTGTACAACACTTCTGATCTGCACAAA
AAACTGGCGATCGCCGCAGCCTCCATCTGGAAGAAAAATCTGGGCGTGGACGTGAAGCTGGTGAACCAGGAGTGGAAAAC
CTTCCTCGATACCCGTCATCAGGGTACCTATGACGTAGCGCGTGCCGGCTGGTGTGCCGACTACAACGAACCAAGCTCGT
TCCTGAACATGATGCTGTCCGACAGCAGCAGTAACACCCCGCACTATAAGAGCGCGGAGTTCGATAAGCTGATGGCGAAC
GTGCTGACGGCGAAAACCAAAGAAGAACGAGCAGATCTGTATCAGAAAGCCGAAGTGCAGCTGGATAAGGATTCGGCCAT
CGTTCCGGTTTATTACTATGTGAATGCCCGTCTGGTGAAACCTTATGTTGGCGGTTACACCGGTAAAGACCCACTTGATA
ACGTGTACGACAAAAATCTGTACATCATCAAGCATTGA

Upstream 100 bases:

>100_bases
TGCCGTATTCGTTCAACGGCCGTCGGCATAACAAGGTCCGCTCACGGGCAACGCAGGTTCCACGACGAGCCTGTGATAAT
AAAAACTGGAGTATACACAC

Downstream 100 bases:

>100_bases
TACGGTAAGTGCCGGCGGTGTAAACCGCCGGCCCATGTTGAGAATAATAAGCCGTTATCAGGCTGCAGCACAGGGTTAGG
GCAATGCTTAAATTTATATT

Product: extracellular solute-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MTNITKKTILAAGIIAALGITATGSAFAAVVPAGVQLADKQEIVKNNGSEVQSLDPHKIEGVPENNVTRDLMEGLANNGP
DGSIVPGVAESWDNKDFKVWTFHLRKDAKWSNGKPVTAQDFVYSWQRVVDPKTASPYASYLQYAHVENVDDVIAGKKDKS
TLGVKAIDDHTFQVTLTEPVPYLVEMTPHYAMKPVFKDAVEKFGEKWTLPANYVSNGAYKLKDWVVNERIVLERNPEYWD
NAKTIINKVTFLPISSEVTDVNRYRTGEIDMTYNNMPIELFQKLKKEIPKEVHVDPYLCTYYYEINNQKAPFTDQRVREA
LKLGLDRDIITNKVKNQGDLPAYSFTPPYTDGAKLTPPEWFGWTQEKRNEVAKKLLAEAGYGPGKPLTFSLLYNTSDLHK
KLAIAAASIWKKNLGVDVKLVNQEWKTFLDTRHQGTYDVARAGWCADYNEPSSFLNMMLSDSSSNTPHYKSAEFDKLMAN
VLTAKTKEERADLYQKAEVQLDKDSAIVPVYYYVNARLVKPYVGGYTGKDPLDNVYDKNLYIIKH

Sequences:

>Translated_545_residues
MTNITKKTILAAGIIAALGITATGSAFAAVVPAGVQLADKQEIVKNNGSEVQSLDPHKIEGVPENNVTRDLMEGLANNGP
DGSIVPGVAESWDNKDFKVWTFHLRKDAKWSNGKPVTAQDFVYSWQRVVDPKTASPYASYLQYAHVENVDDVIAGKKDKS
TLGVKAIDDHTFQVTLTEPVPYLVEMTPHYAMKPVFKDAVEKFGEKWTLPANYVSNGAYKLKDWVVNERIVLERNPEYWD
NAKTIINKVTFLPISSEVTDVNRYRTGEIDMTYNNMPIELFQKLKKEIPKEVHVDPYLCTYYYEINNQKAPFTDQRVREA
LKLGLDRDIITNKVKNQGDLPAYSFTPPYTDGAKLTPPEWFGWTQEKRNEVAKKLLAEAGYGPGKPLTFSLLYNTSDLHK
KLAIAAASIWKKNLGVDVKLVNQEWKTFLDTRHQGTYDVARAGWCADYNEPSSFLNMMLSDSSSNTPHYKSAEFDKLMAN
VLTAKTKEERADLYQKAEVQLDKDSAIVPVYYYVNARLVKPYVGGYTGKDPLDNVYDKNLYIIKH
>Mature_544_residues
TNITKKTILAAGIIAALGITATGSAFAAVVPAGVQLADKQEIVKNNGSEVQSLDPHKIEGVPENNVTRDLMEGLANNGPD
GSIVPGVAESWDNKDFKVWTFHLRKDAKWSNGKPVTAQDFVYSWQRVVDPKTASPYASYLQYAHVENVDDVIAGKKDKST
LGVKAIDDHTFQVTLTEPVPYLVEMTPHYAMKPVFKDAVEKFGEKWTLPANYVSNGAYKLKDWVVNERIVLERNPEYWDN
AKTIINKVTFLPISSEVTDVNRYRTGEIDMTYNNMPIELFQKLKKEIPKEVHVDPYLCTYYYEINNQKAPFTDQRVREAL
KLGLDRDIITNKVKNQGDLPAYSFTPPYTDGAKLTPPEWFGWTQEKRNEVAKKLLAEAGYGPGKPLTFSLLYNTSDLHKK
LAIAAASIWKKNLGVDVKLVNQEWKTFLDTRHQGTYDVARAGWCADYNEPSSFLNMMLSDSSSNTPHYKSAEFDKLMANV
LTAKTKEERADLYQKAEVQLDKDSAIVPVYYYVNARLVKPYVGGYTGKDPLDNVYDKNLYIIKH

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=545, Percent_Identity=75.5963302752294, Blast_Score=866, Evalue=0.0,
Organism=Escherichia coli, GI87081878, Length=531, Percent_Identity=49.1525423728814, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1789397, Length=533, Percent_Identity=42.2138836772983, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI1789966, Length=486, Percent_Identity=25.5144032921811, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1787762, Length=553, Percent_Identity=25.497287522604, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789887, Length=529, Percent_Identity=25.8979206049149, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787052, Length=534, Percent_Identity=22.6591760299625, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787551, Length=540, Percent_Identity=23.8888888888889, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61496; Mature: 61365

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNITKKTILAAGIIAALGITATGSAFAAVVPAGVQLADKQEIVKNNGSEVQSLDPHKIE
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEHHHHHHHHCCCCCCCCCCCCCCC
GVPENNVTRDLMEGLANNGPDGSIVPGVAESWDNKDFKVWTFHLRKDAKWSNGKPVTAQD
CCCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCEEEEEEEEECCCCCCCCCCCCHHH
FVYSWQRVVDPKTASPYASYLQYAHVENVDDVIAGKKDKSTLGVKAIDDHTFQVTLTEPV
HHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEECCCEEEEEECCCC
PYLVEMTPHYAMKPVFKDAVEKFGEKWTLPANYVSNGAYKLKDWVVNERIVLERNPEYWD
CEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEHHEECCEEEEECCCCHHH
NAKTIINKVTFLPISSEVTDVNRYRTGEIDMTYNNMPIELFQKLKKEIPKEVHVDPYLCT
HHHHHHHHEEEEECCCCHHHHHCEECCEEEEEECCCCHHHHHHHHHHCCHHCCCCCEEEE
YYYEINNQKAPFTDQRVREALKLGLDRDIITNKVKNQGDLPAYSFTPPYTDGAKLTPPEW
EEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHH
FGWTQEKRNEVAKKLLAEAGYGPGKPLTFSLLYNTSDLHKKLAIAAASIWKKNLGVDVKL
CCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEE
VNQEWKTFLDTRHQGTYDVARAGWCADYNEPSSFLNMMLSDSSSNTPHYKSAEFDKLMAN
ECHHHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
VLTAKTKEERADLYQKAEVQLDKDSAIVPVYYYVNARLVKPYVGGYTGKDPLDNVYDKNL
HHHHCCHHHHHHHHHHHHEEECCCCCEEEEEEEECCEEECHHHCCCCCCCCCHHHHCCCE
YIIKH
EEEEC
>Mature Secondary Structure 
TNITKKTILAAGIIAALGITATGSAFAAVVPAGVQLADKQEIVKNNGSEVQSLDPHKIE
CCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEHHHHHHHHCCCCCCCCCCCCCCC
GVPENNVTRDLMEGLANNGPDGSIVPGVAESWDNKDFKVWTFHLRKDAKWSNGKPVTAQD
CCCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCEEEEEEEEECCCCCCCCCCCCHHH
FVYSWQRVVDPKTASPYASYLQYAHVENVDDVIAGKKDKSTLGVKAIDDHTFQVTLTEPV
HHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEECCCEEEEEECCCC
PYLVEMTPHYAMKPVFKDAVEKFGEKWTLPANYVSNGAYKLKDWVVNERIVLERNPEYWD
CEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEHHEECCEEEEECCCCHHH
NAKTIINKVTFLPISSEVTDVNRYRTGEIDMTYNNMPIELFQKLKKEIPKEVHVDPYLCT
HHHHHHHHEEEEECCCCHHHHHCEECCEEEEEECCCCHHHHHHHHHHCCHHCCCCCEEEE
YYYEINNQKAPFTDQRVREALKLGLDRDIITNKVKNQGDLPAYSFTPPYTDGAKLTPPEW
EEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHH
FGWTQEKRNEVAKKLLAEAGYGPGKPLTFSLLYNTSDLHKKLAIAAASIWKKNLGVDVKL
CCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEE
VNQEWKTFLDTRHQGTYDVARAGWCADYNEPSSFLNMMLSDSSSNTPHYKSAEFDKLMAN
ECHHHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
VLTAKTKEERADLYQKAEVQLDKDSAIVPVYYYVNARLVKPYVGGYTGKDPLDNVYDKNL
HHHHCCHHHHHHHHHHHHEEECCCCCEEEEEEEECCEEECHHHCCCCCCCCCHHHHCCCE
YIIKH
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2821267; 3525163; 11677609; 8202710; 8747465; 10422831; 10438628 [H]